{"id":"https://openalex.org/W2995806068","doi":"https://doi.org/10.1038/s41598-019-55495-y","title":"Tuning the in vitro sensing and signaling properties of cyanobacterial PII protein by mutation of key residues","display_name":"Tuning the in vitro sensing and signaling properties of cyanobacterial PII protein by mutation of key residues","publication_year":2019,"publication_date":"2019-12-12","ids":{"openalex":"https://openalex.org/W2995806068","doi":"https://doi.org/10.1038/s41598-019-55495-y","mag":"2995806068","pmid":"https://pubmed.ncbi.nlm.nih.gov/31831819","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6908673"},"language":"en","primary_location":{"id":"doi:10.1038/s41598-019-55495-y","doi":"https://doi.org/10.1038/s41598-019-55495-y","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-019-55495-y","pdf_url":"https://www.nature.com/articles/s41598-019-55495-y.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.nature.com/articles/s41598-019-55495-y.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016948718","display_name":"Khaled A. Selim","orcid":"https://orcid.org/0000-0002-2974-9483","observed_orcids":["https://orcid.org/0000-0002-2974-9483"]},"institutions":[{"id":"https://openalex.org/I8087733","display_name":"University of T\u00fcbingen","ror":"https://ror.org/03a1kwz48","country_code":"DE","type":"education","lineage":["https://openalex.org/I8087733"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Khaled A. Selim","raw_affiliation_strings":["Interfaculty Institute of Microbiology and Infection Medicine, Department of Organismic Interactions, Eberhard Karls University of T\u00fcbingen, Auf der Morgenstelle 28, 72076, T\u00fcbingen, Germany. Khaled.selim@uni-tuebingen.de","Interfaculty Institute of Microbiology and Infection Medicine, Department of Organismic Interactions, Eberhard Karls University of T\u00fcbingen, Auf der Morgenstelle 28, 72076, T\u00fcbingen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interfaculty Institute of Microbiology and Infection Medicine, Department of Organismic Interactions, Eberhard Karls University of T\u00fcbingen, Auf der Morgenstelle 28, 72076, T\u00fcbingen, Germany. Khaled.selim@uni-tuebingen.de","institution_ids":["https://openalex.org/I8087733"]},{"raw_affiliation_string":"Interfaculty Institute of Microbiology and Infection Medicine, Department of Organismic Interactions, Eberhard Karls University of T\u00fcbingen, Auf der Morgenstelle 28, 72076, T\u00fcbingen, Germany","institution_ids":["https://openalex.org/I8087733"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059848277","display_name":"Michael C. Haffner","orcid":"https://orcid.org/0000-0001-9906-6832","observed_orcids":["https://orcid.org/0000-0001-9906-6832","https://orcid.org/0000-0003-0809-6425"]},"institutions":[{"id":"https://openalex.org/I8087733","display_name":"University of T\u00fcbingen","ror":"https://ror.org/03a1kwz48","country_code":"DE","type":"education","lineage":["https://openalex.org/I8087733"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Michael Haffner","raw_affiliation_strings":["Interfaculty Institute of Microbiology and Infection Medicine, Department of Organismic Interactions, Eberhard Karls University of T\u00fcbingen, Auf der Morgenstelle 28, 72076, T\u00fcbingen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interfaculty Institute of Microbiology and Infection Medicine, Department of Organismic Interactions, Eberhard Karls University of T\u00fcbingen, Auf der Morgenstelle 28, 72076, T\u00fcbingen, Germany","institution_ids":["https://openalex.org/I8087733"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084347363","display_name":"Bj\u00f6rn Watzer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I8087733","display_name":"University of T\u00fcbingen","ror":"https://ror.org/03a1kwz48","country_code":"DE","type":"education","lineage":["https://openalex.org/I8087733"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Bj\u00f6rn Watzer","raw_affiliation_strings":["Interfaculty Institute of Microbiology and Infection Medicine, Department of Organismic Interactions, Eberhard Karls University of T\u00fcbingen, Auf der Morgenstelle 28, 72076, T\u00fcbingen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interfaculty Institute of Microbiology and Infection Medicine, Department of Organismic Interactions, Eberhard Karls University of T\u00fcbingen, Auf der Morgenstelle 28, 72076, T\u00fcbingen, Germany","institution_ids":["https://openalex.org/I8087733"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5048175299","display_name":"Karl Forchhammer","orcid":"https://orcid.org/0000-0003-3199-8101","observed_orcids":["https://orcid.org/0000-0003-3199-8101"]},"institutions":[{"id":"https://openalex.org/I8087733","display_name":"University of T\u00fcbingen","ror":"https://ror.org/03a1kwz48","country_code":"DE","type":"education","lineage":["https://openalex.org/I8087733"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Karl Forchhammer","raw_affiliation_strings":["Interfaculty Institute of Microbiology and Infection Medicine, Department of Organismic Interactions, Eberhard Karls University of T\u00fcbingen, Auf der Morgenstelle 28, 72076, T\u00fcbingen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interfaculty Institute of Microbiology and Infection Medicine, Department of Organismic Interactions, Eberhard Karls University of T\u00fcbingen, Auf der Morgenstelle 28, 72076, T\u00fcbingen, Germany","institution_ids":["https://openalex.org/I8087733"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5016948718"],"corresponding_institution_ids":["https://openalex.org/I8087733"],"apc_list":{"value":1790,"currency":"USD","value_usd":1790},"apc_paid":{"value":1773,"currency":"EUR","value_usd":1912},"fwci":0.582,"has_fulltext":true,"cited_by_count":28,"citation_normalized_percentile":{"value":0.58738337,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"9","issue":"1","first_page":"18985","last_page":"18985"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.31839999556541443,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.31839999556541443,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.20499999821186066,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.1444000005722046,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pii-protein","display_name":"PII protein","score":0.8840000033378601},{"id":"https://openalex.org/keywords/cyanobacteria","display_name":"cyanobacteria","score":0.972000002861023},{"id":"https://openalex.org/keywords/synechococcus-elongatus-pcc-7942","display_name":"Synechococcus elongatus PCC 7942","score":0.9860000014305115},{"id":"https://openalex.org/keywords/pipx","display_name":"PipX","score":0.9449999928474426},{"id":"https://openalex.org/keywords/adp-binding","display_name":"ADP binding","score":0.8489999771118164},{"id":"https://openalex.org/keywords/atp-binding","display_name":"ATP binding","score":0.7360000014305115},{"id":"https://openalex.org/keywords/salt-bridges","display_name":"salt bridges","score":0.7369999885559082},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.7649999856948853},{"id":"https://openalex.org/keywords/nitrogen-metabolism","display_name":"nitrogen metabolism","score":0.5910000205039978}],"concepts":[{"id":"https://openalex.org/C26517878","wikidata":"https://www.wikidata.org/wiki/Q228039","display_name":"Key (lock)","level":2,"score":0.6860706210136414},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.6016259789466858},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.5890638828277588},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4941597282886505},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.43282178044319153},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.43070098757743835},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4008089303970337},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38135120272636414},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.36533021926879883},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.18353387713432312},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.17211124300956726}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015398","descriptor_name":"Signal Transduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019943","descriptor_name":"Amino Acid Substitution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020125","descriptor_name":"Mutation, Missense","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D046940","descriptor_name":"Synechococcus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D046940","descriptor_name":"Synechococcus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051922","descriptor_name":"PII Nitrogen Regulatory Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D051922","descriptor_name":"PII Nitrogen Regulatory Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1038/s41598-019-55495-y","doi":"https://doi.org/10.1038/s41598-019-55495-y","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-019-55495-y","pdf_url":"https://www.nature.com/articles/s41598-019-55495-y.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:5868306","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6908673","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:31831819","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31831819","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6908673","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC6908673/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sci Rep","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:c63f6dc3389c4ada822f68e47460f4ff","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/c63f6dc3389c4ada822f68e47460f4ff","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1038/s41598-019-55495-y","doi":"https://doi.org/10.1038/s41598-019-55495-y","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-019-55495-y","pdf_url":"https://www.nature.com/articles/s41598-019-55495-y.pdf","source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2334019669","display_name":null,"funder_award_id":"Fo195/9","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"},{"id":"https://openalex.org/G2397324133","display_name":null,"funder_award_id":"Fo195/9-2","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"}],"funders":[{"id":"https://openalex.org/F4320320875","display_name":"Deutscher Akademischer Austauschdienst","ror":"https://ror.org/039djdh30"},{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2995806068.pdf","grobid_xml":"https://content.openalex.org/works/W2995806068.grobid-xml"},"referenced_works_count":31,"referenced_works":["https://openalex.org/W1987172632","https://openalex.org/W2011527037","https://openalex.org/W2012484130","https://openalex.org/W2013939529","https://openalex.org/W2021952073","https://openalex.org/W2029836252","https://openalex.org/W2034274882","https://openalex.org/W2055540135","https://openalex.org/W2066109365","https://openalex.org/W2084099882","https://openalex.org/W2098576269","https://openalex.org/W2102036522","https://openalex.org/W2106041483","https://openalex.org/W2109610790","https://openalex.org/W2121950348","https://openalex.org/W2124220073","https://openalex.org/W2129558617","https://openalex.org/W2141327755","https://openalex.org/W2145404025","https://openalex.org/W2160538707","https://openalex.org/W2177154639","https://openalex.org/W2535992996","https://openalex.org/W2609327219","https://openalex.org/W2783135910","https://openalex.org/W2803852299","https://openalex.org/W2898411802","https://openalex.org/W2900940038","https://openalex.org/W2922092399","https://openalex.org/W2955662042","https://openalex.org/W2959733893","https://openalex.org/W2980534943"],"related_works":["https://openalex.org/W3094616298","https://openalex.org/W1987172632","https://openalex.org/W2158267014","https://openalex.org/W2012484130","https://openalex.org/W2900940038","https://openalex.org/W7099359350","https://openalex.org/W2113199654","https://openalex.org/W2133934199","https://openalex.org/W2595551057","https://openalex.org/W7039808418"],"abstract_inverted_index":{"PII":[0,19,45,60,89,135,143,195],"proteins":[1,20,46],"comprise":[2],"an":[3],"ancient":[4],"superfamily":[5],"of":[6,15,28,34,75,86,134,153,161,169,184,191],"signal":[7],"transduction":[8],"proteins,":[9],"widely":[10],"distributed":[11],"among":[12,62],"all":[13],"domains":[14],"life.":[16],"In":[17,39,58],"general,":[18],"measure":[21],"and":[22,37,55,72,164],"integrate":[23],"the":[24,29,64,73,76,81,87,104,151,167,170,176,182,189],"current":[25],"carbon/nitrogen/energy":[26],"status":[27],"cell":[30],"through":[31],"interdependent":[32],"binding":[33,155,160],"ATP,":[35],"ADP":[36],"2-oxogluterate.":[38],"response":[40],"to":[41,51,173,197],"effector":[42],"molecule":[43],"binding,":[44],"interact":[47],"with":[48,113,123],"various":[49],"PII-receptors":[50],"tune":[52],"central":[53],"carbon-":[54],"nitrogen":[56],"metabolism.":[57,199],"cyanobacteria,":[59],"regulates,":[61],"others,":[63],"key":[65,139],"enzyme":[66],"for":[67,141,181],"nitrogen-storage,":[68],"N-acetyl-glutamate":[69],"kinase":[70],"(NAGK),":[71],"co-activator":[74],"global":[77],"nitrogen-trascription":[78],"factor":[79],"NtcA,":[80],"PII-interacting":[82],"protein-X":[83],"(PipX).":[84],"One":[85],"remarkable":[88],"variants":[90,196],"from":[91],"Synechococcus":[92],"elongatus":[93],"PCC":[94],"7942":[95],"that":[96,132],"yielded":[97],"mechanistic":[98],"insights":[99],"in":[100,125,175],"PII-NAGK":[101],"interaction,":[102],"is":[103,118,137],"NAGK-superactivating":[105],"variant":[106,178],"I86N.":[107],"Here":[108],"we":[109,130],"studied":[110],"its":[111],"interaction":[112],"PipX.":[114,165],"Another":[115],"critical":[116],"residue":[117,140],"Lys58,":[119],"forming":[120],"a":[121,126,138],"salt-bridge":[122],"2-oxoglutarate":[124],"PII-ATP-2-oxoglutarate":[127],"complex.":[128],"Here,":[129],"show":[131],"Lys58":[133],"protein":[136],"mediating":[142],"interactions.":[144],"The":[145],"K58N":[146,177],"mutation":[147],"not":[148],"only":[149],"causes":[150],"loss":[152,183],"2-oxogluterate":[154],"but":[156],"also":[157],"strongly":[158],"impairs":[159],"ADP,":[162],"NAGK":[163],"Remarkably,":[166],"exchange":[168],"nearby":[171],"Leu56":[172],"Lys":[174],"partially":[179],"compensates":[180],"K58.":[185],"This":[186],"study":[187],"demonstrates":[188],"potential":[190],"creating":[192],"custom":[193],"tailored":[194],"modulate":[198]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2019-12-26T00:00:00"}