{"id":"https://openalex.org/W2995725100","doi":null,"title":"Development of a HEK293 Cell Line to Show Inhibition of Tau Aggregation","display_name":"Development of a HEK293 Cell Line to Show Inhibition of Tau Aggregation","publication_year":2019,"publication_date":"2019-01-01","ids":{"openalex":"https://openalex.org/W2995725100","mag":"2995725100"},"language":"en","primary_location":{"id":"pmh:oai:digitalcommons.du.edu:etd-2617","doi":null,"is_oa":true,"landing_page_url":"https://digitalcommons.du.edu/etd/1617","pdf_url":"https://digitalcommons.du.edu/etd/1617","source":{"id":"https://openalex.org/S4377196520","display_name":"Digital Commons - DU (University of Denver)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I131651094","host_organization_name":"University of Denver","host_organization_lineage":["https://openalex.org/I131651094"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Theses and Dissertations","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://digitalcommons.du.edu/etd/1617","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001079937","display_name":"Justin Ray Shady","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Justin Ray Shady","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5001079937"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10086","display_name":"Alzheimer's disease research and treatments","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10009","display_name":"Dementia and Cognitive Impairment Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2738","display_name":"Psychiatry and Mental health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tau-aggregation","display_name":"tau aggregation","score":0.9520000219345093},{"id":"https://openalex.org/keywords/tauopathy","display_name":"tauopathy","score":0.75},{"id":"https://openalex.org/keywords/alzheimer-s-disease","display_name":"Alzheimer's disease","score":0.9919999837875366},{"id":"https://openalex.org/keywords/map2","display_name":"MAP2","score":0.7699999809265137},{"id":"https://openalex.org/keywords/map2c","display_name":"MAP2c","score":0.8240000009536743},{"id":"https://openalex.org/keywords/templated-growth","display_name":"template-assisted growth","score":0.8349999785423279},{"id":"https://openalex.org/keywords/tau-isoforms","display_name":"tau isoforms","score":0.7459999918937683},{"id":"https://openalex.org/keywords/tau-hyperphosphorylation","display_name":"tau hyperphosphorylation","score":0.6790000200271606},{"id":"https://openalex.org/keywords/hek293-cells","display_name":"HEK293 cells","score":0.859000027179718},{"id":"https://openalex.org/keywords/tau-aggregation-inhibitors","display_name":"tau aggregation inhibitors","score":0.34599998593330383},{"id":"https://openalex.org/keywords/microtubule-binding-repeats","display_name":"microtubule-binding repeats","score":0.7749999761581421}],"concepts":[{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.6283158659934998},{"id":"https://openalex.org/C198352243","wikidata":"https://www.wikidata.org/wiki/Q37105","display_name":"Line (geometry)","level":2,"score":0.46136680245399475},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3971453905105591},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.29990774393081665},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2601001262664795},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2195034623146057},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.15454897284507751},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:digitalcommons.du.edu:etd-2617","doi":null,"is_oa":true,"landing_page_url":"https://digitalcommons.du.edu/etd/1617","pdf_url":"https://digitalcommons.du.edu/etd/1617","source":{"id":"https://openalex.org/S4377196520","display_name":"Digital Commons - DU (University of Denver)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I131651094","host_organization_name":"University of Denver","host_organization_lineage":["https://openalex.org/I131651094"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Theses and Dissertations","raw_type":"text"},{"id":"mag:2995725100","doi":null,"is_oa":false,"landing_page_url":"https://digitalcommons.du.edu/cgi/viewcontent.cgi?article=2617&context=etd","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:digitalcommons.du.edu:etd-2617","doi":null,"is_oa":true,"landing_page_url":"https://digitalcommons.du.edu/etd/1617","pdf_url":"https://digitalcommons.du.edu/etd/1617","source":{"id":"https://openalex.org/S4377196520","display_name":"Digital Commons - DU (University of Denver)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I131651094","host_organization_name":"University of Denver","host_organization_lineage":["https://openalex.org/I131651094"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Theses and Dissertations","raw_type":"text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.766700029373169,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2995725100.pdf","grobid_xml":"https://content.openalex.org/works/W2995725100.grobid-xml"},"referenced_works_count":53,"referenced_works":["https://openalex.org/W70729731","https://openalex.org/W1484326305","https://openalex.org/W1509571186","https://openalex.org/W1600934558","https://openalex.org/W1968232509","https://openalex.org/W1973855353","https://openalex.org/W1989208474","https://openalex.org/W1989390298","https://openalex.org/W1995737263","https://openalex.org/W1997942761","https://openalex.org/W2001991040","https://openalex.org/W2005755021","https://openalex.org/W2007428318","https://openalex.org/W2015115860","https://openalex.org/W2036008741","https://openalex.org/W2036294565","https://openalex.org/W2040351396","https://openalex.org/W2041655987","https://openalex.org/W2059809766","https://openalex.org/W2060904588","https://openalex.org/W2066052286","https://openalex.org/W2066847680","https://openalex.org/W2068009750","https://openalex.org/W2071397750","https://openalex.org/W2075813074","https://openalex.org/W2081966615","https://openalex.org/W2091441518","https://openalex.org/W2099540110","https://openalex.org/W2104034347","https://openalex.org/W2118608964","https://openalex.org/W2118929543","https://openalex.org/W2119310960","https://openalex.org/W2125012012","https://openalex.org/W2125022522","https://openalex.org/W2126424761","https://openalex.org/W2137586320","https://openalex.org/W2143306707","https://openalex.org/W2158118162","https://openalex.org/W2158314129","https://openalex.org/W2159333258","https://openalex.org/W2159377066","https://openalex.org/W2170888377","https://openalex.org/W2340580074","https://openalex.org/W2545421204","https://openalex.org/W2595372664","https://openalex.org/W2617946428","https://openalex.org/W2769171126","https://openalex.org/W2777639897","https://openalex.org/W2781579290","https://openalex.org/W2802245552","https://openalex.org/W2872898993","https://openalex.org/W2891635110","https://openalex.org/W2892916393"],"related_works":["https://openalex.org/W7027486002","https://openalex.org/W4379468411","https://openalex.org/W2535321677","https://openalex.org/W7207584333","https://openalex.org/W2800294938","https://openalex.org/W6983495089","https://openalex.org/W1984770038","https://openalex.org/W1593693427","https://openalex.org/W4230584396","https://openalex.org/W2075547607"],"abstract_inverted_index":{"Intracellular":[0],"deposition":[1],"of":[2,8,15,41,89,98,101,142,152,166,190,251,261,282,289,301,325],"aggregated":[3],"tau":[4,56,153,169,184,191,217,224,238,327],"is":[5,21,33,148,156],"the":[6,12,39,85,94,99,132,140,188,203,299,317],"hallmark":[7],"several":[9,52],"different":[10],"tauopathies,":[11],"most":[13],"widespread":[14],"these":[16,208],"being":[17],"Alzheimer's":[18,130,145],"disease.":[19,146],"Tau":[20,78],"a":[22,72,149,222,236,310],"highly":[23],"soluble,":[24],"intrinsically":[25],"disordered,":[26],"microtubule":[27,36,107,136,162,172],"associated":[28],"protein.":[29],"Tau's":[30],"native":[31],"function":[32],"to":[34,57,177,187,201,258,277,297],"stabilize":[35],"formation":[37,284],"in":[38,71,161,185,279,331,334],"axons":[40],"neurons.":[42],"Post":[43],"translational":[44],"modification":[45],"such":[46],"as":[47,49,51,75,116,126],"hyperphosphorylation":[48],"well":[50],"familial":[53],"mutations":[54],"allow":[55],"nucleate":[58],"and":[59,93,119,134,154,159,168,193,234,255,292,307,321,333],"form":[60,139],"fibrils.":[61],"These":[62],"fibrils":[63,143,192],"can":[64],"recruit":[65],"healthy":[66],"monomers":[67],"onto":[68],"their":[69,171],"ends":[70,189],"fashion":[73],"described":[74,115,125],"template-assisted":[76],"growth.":[77],"has":[79],"6":[80],"isoforms":[81,118],"that":[82,319],"vary":[83],"by":[84,285,305],"inclusion":[86,95],"or":[87,96],"exclusion":[88,97],"two":[90,210,216,286],"N-terminal":[91],"repeats":[92,104,113,123,138,174],"second":[100],"four":[102,122],"semiconserved":[103],"within":[105,144,170],"its":[106],"binding":[108,137,173,186],"region.":[109],"Isoforms":[110],"containing":[111,121,303],"three":[112],"are":[114,124,323],"3R":[117,150],"those":[120],"4R":[127,157],"isoforms.":[128],"In":[129],"disease":[131],"3rd":[133],"4th":[135],"core":[141],"MAP2C":[147],"homologue":[151,158],"MAP2D":[155],"assists":[160],"stabilization.":[163],"The":[164],"homology":[165],"MAP2":[167,180],"gave":[175],"motivation":[176],"investigate":[178],"whether":[179],"could":[181],"compete":[182],"with":[183,228,242,267],"inhibit":[194],"elongation.":[195],"Preliminary":[196],"in-vitro":[197],"data":[198,314],"suggest":[199],"this":[200],"be":[202,259],"case.":[204],"To":[205],"further":[206,315],"strengthen":[207],"findings,":[209],"HEK293":[211],"cell":[212,249],"lines":[213,250],"stably":[214],"expressing":[215],"constructs":[218],"were":[219,253,256,274],"established;":[220],"hT40P301S-EYFP,":[221],"full-length":[223],"construct":[225,239],"C-terminally":[226,240],"tagged":[227,241],"an":[229,243],"Enhanced":[230,244],"Yellow":[231,245],"Fluorescence":[232,246],"Protein,":[233],"K18P301S-EYFP,":[235],"truncated":[237],"Protein.":[247],"Monoclonal":[248,270],"hT40P301S-EYFP":[252],"selected":[254],"shown":[257],"capable":[260,324],"forming":[262],"intracellular":[263],"puncta":[264,304],"when":[265],"transfected":[266,272],"K18wt":[268],"seeds.":[269],"hT40P301S-YFP":[271],"cells":[273,302],"then":[275],"used":[276],"show":[278],"culture":[280],"inhibition":[281],"fibril":[283,328],"truncations":[287],"(tr)":[288],"MAP2,":[290],"MAP2Ctr":[291,294,320],"MAP2Dtr.":[293],"was":[295],"able":[296],"decrease":[298],"number":[300],"33%":[306],"MAP2Dtr":[308,322],"showed":[309],"54%":[311],"decrease.":[312],"This":[313],"supports":[316],"findings":[318],"inhibiting":[326],"elongation":[329],"both":[330],"vitro":[332],"culture.":[335]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2019-12-26T00:00:00"}
