{"id":"https://openalex.org/W2995463523","doi":"https://doi.org/10.21769/bioprotoc.3459","title":"Propagation and Purification of Chlamydia trachomatis Serovar L2 Transformants and Mutants","display_name":"Propagation and Purification of Chlamydia trachomatis Serovar L2 Transformants and Mutants","publication_year":2019,"publication_date":"2019-01-01","ids":{"openalex":"https://openalex.org/W2995463523","doi":"https://doi.org/10.21769/bioprotoc.3459","mag":"2995463523","pmid":"https://pubmed.ncbi.nlm.nih.gov/33654954"},"language":"en","primary_location":{"id":"doi:10.21769/bioprotoc.3459","doi":"https://doi.org/10.21769/bioprotoc.3459","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.3459","pdf_url":"https://bio-protocol.org/pdf/Bio-protocol3459.pdf","source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://bio-protocol.org/pdf/Bio-protocol3459.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057439083","display_name":"Robert Faris","orcid":"https://orcid.org/0000-0003-0855-7041","observed_orcids":["https://orcid.org/0000-0003-0855-7041"]},"institutions":[{"id":"https://openalex.org/I126307644","display_name":"University of Iowa","ror":"https://ror.org/036jqmy94","country_code":"US","type":"education","lineage":["https://openalex.org/I126307644"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert Faris","raw_affiliation_strings":["Microbiology and Immunology, University of Iowa, Carver College of Medicine, Iowa City, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microbiology and Immunology, University of Iowa, Carver College of Medicine, Iowa City, U.S.A","institution_ids":["https://openalex.org/I126307644"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5082978300","display_name":"Mary M. Weber","orcid":"https://orcid.org/0000-0001-5126-2164","observed_orcids":["https://orcid.org/0000-0001-5126-2164"]},"institutions":[{"id":"https://openalex.org/I126307644","display_name":"University of Iowa","ror":"https://ror.org/036jqmy94","country_code":"US","type":"education","lineage":["https://openalex.org/I126307644"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Mary Weber","raw_affiliation_strings":["Microbiology and Immunology, University of Iowa, Carver College of Medicine, Iowa City, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microbiology and Immunology, University of Iowa, Carver College of Medicine, Iowa City, U.S.A","institution_ids":["https://openalex.org/I126307644"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5082978300"],"corresponding_institution_ids":["https://openalex.org/I126307644"],"apc_list":null,"apc_paid":null,"fwci":0.4866,"has_fulltext":true,"cited_by_count":17,"citation_normalized_percentile":{"value":0.65811441,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"9","issue":"24","first_page":"e3459","last_page":"e3459"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10428","display_name":"Reproductive tract infections research","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10428","display_name":"Reproductive tract infections research","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.004999999888241291,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.0034000000450760126,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lymphogranuloma-venereum","display_name":"lymphogranuloma venereum","score":0.8939999938011169},{"id":"https://openalex.org/keywords/gastrografin","display_name":"Gastrografin","score":0.7559999823570251},{"id":"https://openalex.org/keywords/diatrizoate-meglumine","display_name":"meglumine diatrizoate","score":0.9710000157356262},{"id":"https://openalex.org/keywords/allelic-exchange","display_name":"allelic exchange","score":0.9869999885559082},{"id":"https://openalex.org/keywords/host-cell-infection","display_name":"host cell infection","score":0.5400000214576721}],"concepts":[{"id":"https://openalex.org/C2777391075","wikidata":"https://www.wikidata.org/wiki/Q131065","display_name":"Chlamydia trachomatis","level":2,"score":0.9254130125045776},{"id":"https://openalex.org/C2776334974","wikidata":"https://www.wikidata.org/wiki/Q694552","display_name":"Lymphogranuloma venereum","level":3,"score":0.7755676507949829},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7012587189674377},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6243537068367004},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5594797134399414},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.512815535068512},{"id":"https://openalex.org/C2780122209","wikidata":"https://www.wikidata.org/wiki/Q153356","display_name":"Chlamydia","level":2,"score":0.5029508471488953},{"id":"https://openalex.org/C24990904","wikidata":"https://www.wikidata.org/wiki/Q7075144","display_name":"Obligate","level":2,"score":0.5023255348205566},{"id":"https://openalex.org/C2777038808","wikidata":"https://www.wikidata.org/wiki/Q13474598","display_name":"Chlamydiaceae","level":3,"score":0.4983992576599121},{"id":"https://openalex.org/C10389963","wikidata":"https://www.wikidata.org/wiki/Q848328","display_name":"Serotype","level":2,"score":0.49651890993118286},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.47542527318000793},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.4615177810192108},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.22923988103866577},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.21540167927742004},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.18962085247039795},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.21769/bioprotoc.3459","doi":"https://doi.org/10.21769/bioprotoc.3459","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.3459","pdf_url":"https://bio-protocol.org/pdf/Bio-protocol3459.pdf","source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},{"id":"pmid:33654954","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33654954","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bio-protocol","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7854018","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7854018","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Bio Protoc","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:0d3ccdce2b864c2dbb52e9e88b783974","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/0d3ccdce2b864c2dbb52e9e88b783974","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Bio-Protocol, Vol 9, Iss 24 (2019)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.21769/bioprotoc.3459","doi":"https://doi.org/10.21769/bioprotoc.3459","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.3459","pdf_url":"https://bio-protocol.org/pdf/Bio-protocol3459.pdf","source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.46810001134872437,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[{"score":0.4399999976158142,"display_name":"Decent work and economic growth","id":"https://metadata.un.org/sdg/8"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2995463523.pdf","grobid_xml":"https://content.openalex.org/works/W2995463523.grobid-xml"},"referenced_works_count":7,"referenced_works":["https://openalex.org/W1839636646","https://openalex.org/W1956578576","https://openalex.org/W2079805712","https://openalex.org/W2160019429","https://openalex.org/W2168591865","https://openalex.org/W2275021215","https://openalex.org/W2341248966"],"related_works":["https://openalex.org/W2143582175","https://openalex.org/W2312501654","https://openalex.org/W2056790637","https://openalex.org/W4223571743","https://openalex.org/W2340725185","https://openalex.org/W2993981509","https://openalex.org/W2074571538","https://openalex.org/W2147203143","https://openalex.org/W2288646582","https://openalex.org/W2165566963"],"abstract_inverted_index":{"Chlamydia":[0,173],"trachomatis":[1,174],"(C.t.)":[2],"is":[3,48,62,108,126,138],"an":[4],"obligate":[5],"intracellular":[6],"pathogen":[7],"that":[8,28],"cannot":[9],"be":[10,15],"cultured":[11],"axenically":[12],"and":[13,39,59,61,85,103,112,141,170],"must":[14],"propagated":[16],"within":[17],"eukaryotic":[18],"host":[19],"cells.There":[20],"are":[21],"at":[22],"least":[23],"15":[24],"distinct":[25],"chlamydial":[26,158],"serovariants":[27],"belong":[29],"to":[30,36,72,80,182],"2":[31],"major":[32],"biovars":[33],"commonly":[34],"referred":[35],"as":[37,88,90,143,145],"trachoma":[38],"lymphogranuloma":[40],"venereum":[41],"(LGV).The":[42],"invasive":[43],"chlamydia":[44],"LGV":[45],"serovar":[46,175],"L2":[47,76,176],"the":[49,63,98,115,122],"most":[50,116],"widely":[51],"used":[52,120],"experimental":[53],"model":[54],"for":[55,157,166],"studying":[56],"C.t.":[57,75,107],"biology":[58],"infection":[60],"only":[64],"strain":[65],"with":[66,177],"reliable":[67],"genetic":[68],"tools":[69],"available.New":[70],"techniques":[71],"genetically":[73],"manipulate":[74],"have":[77,147],"provided":[78],"opportunities":[79],"make":[81],"mutants":[82,183],"using":[83,151],"TargeTron":[84],"allelic":[86],"exchange":[87],"well":[89,144],"strains":[91],"overexpressing":[92],"epitope-tagged":[93],"proteins,":[94],"in":[95,121,153],"turn":[96],"necessitating":[97],"regular":[99],"purification":[100,123],"of":[101,106,114,133,155,172],"transformant":[102],"mutant":[104],"clones.Purification":[105],"a":[109,162],"laborintensive":[110],"exercise":[111],"one":[113],"common":[117],"reagents":[118],"classically":[119],"process,":[124],"Renografin,":[125],"no":[127],"longer":[128],"commercially":[129],"available.A":[130],"similar":[131],"formulation":[132],"diatrizoate":[134],"meglumine":[135],"called":[136],"Gastrografin":[137],"readily":[139],"available":[140],"we":[142,160],"others":[146],"had":[148],"great":[149],"success":[150],"this":[152],"place":[154],"Renografin":[156],"purifications.Here,":[159],"provide":[161],"detailed":[163],"general":[164],"protocol":[165],"infection,":[167],"propagation,":[168],"purification,":[169],"titering":[171],"additional":[178],"notes":[179],"specifically":[180],"pertaining":[181],"or":[184],"recombinant":[185],"DNA":[186],"carrying":[187],"clones.":[188]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1}],"updated_date":"2026-10-08T09:06:37.294493","created_date":"2025-10-10T00:00:00"}