{"id":"https://openalex.org/W2995401601","doi":null,"title":"Evaluating barramundi (Lates calcarifer) sperm quality using high throughput advanced reproductive tools","display_name":"Evaluating barramundi (Lates calcarifer) sperm quality using high throughput advanced reproductive tools","publication_year":2019,"publication_date":"2019-01-01","ids":{"openalex":"https://openalex.org/W2995401601","mag":"2995401601"},"language":"en","primary_location":{"id":"pmh:oai:researchonline.jcu.edu.au:59825","doi":null,"is_oa":false,"landing_page_url":"https://researchonline.jcu.edu.au/59825/1/Conference_Abstract_AM%20%28accepted%29.pdf","pdf_url":"https://researchonline.jcu.edu.au/59825/1/Conference_Abstract_AM%20%28accepted%29.pdf","source":{"id":"https://openalex.org/S4306400519","display_name":"ResearchOnline at James Cook University (James Cook University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86467917","host_organization_name":"James Cook University","host_organization_lineage":["https://openalex.org/I86467917"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Conference Item"},"volume":null,"type":"conference-paper","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5067961386","display_name":"Adrien F. Marc","orcid":"https://orcid.org/0000-0002-9364-6438","observed_orcids":["https://orcid.org/0000-0002-9364-6438"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Adrien F. Marc","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-9364-6438","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039274530","display_name":"Jarrod L. Guppy","orcid":"https://orcid.org/0000-0003-1864-9644","observed_orcids":["https://orcid.org/0000-0003-1864-9644"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jarrod L. Guppy","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-1864-9644","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011305538","display_name":"Dean Robert Jerry","orcid":"https://orcid.org/0000-0003-3735-1798","observed_orcids":["https://orcid.org/0000-0003-3735-1798"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dean R. Jerry","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-3735-1798","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112492318","display_name":"Peter Mulvey","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Peter Mulvey","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026106607","display_name":"Paige Bauer","orcid":"https://orcid.org/0000-0001-9781-1480","observed_orcids":["https://orcid.org/0000-0001-9781-1480"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Paige Bauer","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-9781-1480","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5071029130","display_name":"Damien B.B.P. Paris","orcid":"https://orcid.org/0000-0001-7329-8985","observed_orcids":["https://orcid.org/0000-0001-7329-8985"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Damien B.B.P. Paris","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-7329-8985","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.9829000234603882,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11505","display_name":"Reproductive biology and impacts on aquatic species","score":0.9829000234603882,"subfield":{"id":"https://openalex.org/subfields/1314","display_name":"Physiology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.011599999852478504,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10450","display_name":"Aquaculture Nutrition and Growth","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1104","display_name":"Aquatic Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lates-calcarifer","display_name":"Lates calcarifer","score":0.9959999918937683},{"id":"https://openalex.org/keywords/sperm-quality","display_name":"semen quality","score":0.9580000042915344},{"id":"https://openalex.org/keywords/male-fertility","display_name":"male fertility","score":0.843999981880188},{"id":"https://openalex.org/keywords/sperm-morphology","display_name":"sperm morphology","score":0.6930000185966492},{"id":"https://openalex.org/keywords/sperm-motility","display_name":"sperm motility","score":0.8399999737739563},{"id":"https://openalex.org/keywords/sperm-viability","display_name":"sperm viability","score":0.75},{"id":"https://openalex.org/keywords/sperm-dna-fragmentation","display_name":"sperm DNA fragmentation","score":0.8230000138282776},{"id":"https://openalex.org/keywords/sperm-cryopreservation","display_name":"sperm cryopreservation","score":0.47200000286102295},{"id":"https://openalex.org/keywords/computer-assisted-sperm-analysis","display_name":"computer-assisted sperm analysis","score":0.7059999704360962},{"id":"https://openalex.org/keywords/paternity-skew","display_name":"paternity skew","score":0.6510000228881836},{"id":"https://openalex.org/keywords/broodstock","display_name":"broodstock","score":0.5609999895095825},{"id":"https://openalex.org/keywords/fish-reproduction","display_name":"fish reproduction","score":0.36899998784065247}],"concepts":[{"id":"https://openalex.org/C2780805331","wikidata":"https://www.wikidata.org/wiki/Q74411","display_name":"Barramundi","level":4,"score":0.9604274034500122},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.7277092933654785},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7029352188110352},{"id":"https://openalex.org/C2778937986","wikidata":"https://www.wikidata.org/wiki/Q787185","display_name":"Lates","level":3,"score":0.6951701045036316},{"id":"https://openalex.org/C110175623","wikidata":"https://www.wikidata.org/wiki/Q4284904","display_name":"Broodstock","level":4,"score":0.5927172303199768},{"id":"https://openalex.org/C505870484","wikidata":"https://www.wikidata.org/wiki/Q180538","display_name":"Fishery","level":1,"score":0.5204039812088013},{"id":"https://openalex.org/C86909935","wikidata":"https://www.wikidata.org/wiki/Q188989","display_name":"Aquaculture","level":3,"score":0.44548124074935913},{"id":"https://openalex.org/C90856448","wikidata":"https://www.wikidata.org/wiki/Q431","display_name":"Zoology","level":1,"score":0.43403464555740356},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.33614081144332886},{"id":"https://openalex.org/C2909208804","wikidata":"https://www.wikidata.org/wiki/Q127282","display_name":"Fish <Actinopterygii>","level":2,"score":0.09875321388244629},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:researchonline.jcu.edu.au:59825","doi":null,"is_oa":false,"landing_page_url":"https://researchonline.jcu.edu.au/59825/1/Conference_Abstract_AM%20%28accepted%29.pdf","pdf_url":"https://researchonline.jcu.edu.au/59825/1/Conference_Abstract_AM%20%28accepted%29.pdf","source":{"id":"https://openalex.org/S4306400519","display_name":"ResearchOnline at James Cook University (James Cook University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86467917","host_organization_name":"James Cook University","host_organization_lineage":["https://openalex.org/I86467917"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Conference Item"},{"id":"mag:2995401601","doi":null,"is_oa":false,"landing_page_url":"https://researchonline.jcu.edu.au/59825/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/14","score":1.0,"display_name":"Life below water"},{"id":"https://metadata.un.org/sdg/2","score":0.5666999816894531,"display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3048052127","https://openalex.org/W2046911221","https://openalex.org/W4392128314","https://openalex.org/W6889659973","https://openalex.org/W83930161","https://openalex.org/W629554038","https://openalex.org/W3093959201","https://openalex.org/W4387358846","https://openalex.org/W4210433090","https://openalex.org/W2767194011"],"abstract_inverted_index":{"Introduction:":[0],"Barramundi,":[1],"or":[2,467,475,512,739],"Asian":[3],"sea":[4],"bass":[5],"(Lates":[6],"calcarifer),":[7],"is":[8,108,714],"an":[9,356],"emerging":[10],"species":[11,107,703],"for":[12,20,105,644,757],"aquaculture":[13],"worldwide":[14],"(ABARES,":[15],"2013).":[16,77],"The":[17],"growing":[18],"demand":[19],"barramundi":[21,26,31,124,200,344,403,589,655,679],"fillets":[22],"necessitates":[23],"intensification":[24],"of":[25,42,66,88,113,141,199,247,252,285,355,406,471,479,489,524,554,566,595,636,654,666,678,692,698,701,720,743],"production.":[27],"Breeding":[28],"practices":[29],"in":[30,63,94,127,161,167,188,687,747,763],"farming":[32],"have":[33,473,632],"not":[34,497],"evolved":[35],"since":[36,502],"the":[37,40,111,244,250,336,397,407,415,441,550,581,634,647,718,741,754],"1980s":[38],"with":[39,171,414,546],"introduction":[41],"hormonal":[43],"therapy":[44],"to":[45,55,90,97,101,117,122,129,193,196,221,256,262,341,401,449,485,668,696,716,723],"trigger":[46],"group-spawning":[47],"events.":[48],"This":[49],"group":[50],"mating":[51],"strategy":[52],"often":[53,61],"leads":[54],"highly":[56,412,507,544,758],"skewed":[57,759],"paternity":[58,760],"among":[59],"offspring,":[60],"resulting":[62],"a":[64,86,177,280,306,351,374,384,391,661,675],"loss":[65],"valuable":[67],"genetic":[68],"diversity":[69],"(Frost":[70],"et":[71,75,705,709],"al.,":[72,76,706,710],"2006;":[73],"Loughnan":[74],"With":[78],"few":[79],"studies":[80],"available":[81],"on":[82,133,152,176,205,213,388,396,580],"male":[83,142,158,588],"fertility":[84],"and":[85,145,216,223,228,249,279,290,373,438,584,607,617,625,641,651,663],"lack":[87],"technology":[89],"quantify":[91],"sperm":[92,125,135,226,233,258,325,345,409,445,462,500,504,537,593,671,683,731],"quality":[93,126,594,684,745],"barramundi,":[95],"efforts":[96],"develop":[98,118],"reproductive":[99],"tools":[100],"obtain":[102],"such":[103,239,721],"knowledge":[104],"this":[106,114,217,688],"warranted.":[109],"Thus,":[110],"aim":[112],"study":[115,689],"was":[116,209,219,272,321,394,411,496,506,543,727,740],"high":[119],"throughput":[120],"techniques":[121],"evaluate":[123],"order":[128],"provide":[130],"preliminary":[131],"information":[132],"basic":[134],"biology":[136],"that":[137,697,750],"allows":[138],"future":[139],"investigation":[140],"fertility.":[143],"Materials":[144],"methods:":[146],"Spermatozoa":[147],"were":[148,186,254,339,527,569],"collected":[149],"by":[150,211,231,294,328,383,499,533,540,576,730],"catheterization":[151],"multiple":[153],"occasions":[154],"from":[155,433,765],"n=15":[156],"mature":[157],"broodstock":[159,590],"maintained":[160],"breeding":[162],"condition":[163],"at":[164,173,427],"30":[165,174],"\u00b0C":[166,276],"28,000":[168],"l":[169],"tanks,":[170],"salinity":[172],"ppt":[175],"16":[178],"h":[179,182],"light:":[180],"8":[181],"dark":[183],"cycle.":[184],"Samples":[185],"diluted":[187],"marine":[189],"Ringer\u2019s":[190],"solution,":[191],"adjusted":[192],"400":[194],"mOsm":[195],"mimic":[197],"osmolality":[198],"seminal":[201],"plasma,":[202],"then":[203],"held":[204],"ice.":[206],"Sperm":[207],"morphology":[208],"measured":[210],"ImageJ":[212],"SpermBlue-stained":[214],"smears,":[215],"data":[218,685],"used":[220,340],"calibrate":[222],"validate":[224],"automated":[225,408,444,503],"counting":[227,417],"motility":[229,253,501],"assessment":[230,665,677],"computer-assisted":[232],"analysis":[234],"(CASA;":[235],"Androvision,":[236],"Minitube).":[237],"Factors":[238],"as":[240,529,571],"optimal":[241],"sample":[242],"dilution,":[243],"minimum":[245],"number":[246],"fields,":[248],"effect":[251],"examined":[255],"determine":[257,724],"detection":[259,316,392,494],"accuracy":[260,495],"compared":[261,448,484],"manual":[263,416,450,486],"haemocytometer":[264,451,487],"methods.":[265],"A":[266],"Hoechst/propidium":[267],"iodide":[268],"(PI)":[269],"viability":[270,538,548,650],"assay":[271,311,542],"validated":[273,322,582,633],"using":[274,323,465,510,549],"70":[275],"heat-treated":[277,525],"controls":[278,326],"5-point":[281,551],"intact:damaged":[282,555],"dilution":[283,426,552],"curve":[284,553],"0:100,":[286],"25:75,":[287],"50:50,":[288],"75:25":[289],"100:0%":[291],"spermatozoa":[292,526,556,568,700,746],"assessed":[293,327],"flow":[295,329,534,577,642],"cytometry":[296,330,643],"(\u2265100":[297,331],"000":[298,332,670],"cells;":[299],"FACS":[300],"Canto":[301],"II,":[302],"BD":[303],"Biosciences).":[304],"Lastly,":[305,562],"FITC/PI":[307],"TUNEL":[308,640],"DNA":[309,572,621,652],"fragmentation":[310],"(In":[312],"situ":[313],"cell":[314],"death":[315],"kit":[317],"-":[318],"fluorescein,":[319],"Roche)":[320],"DNase-treated":[324,567],"cells).":[333],"Once":[334],"validated,":[335],"optimised":[337],"assays":[338,583,659],"characterise":[342],"baseline":[343,592],"quality.":[346],"Results:":[347],"Barramundi":[348],"spermatozoon":[349],"has":[350],"primitive":[352],"structure,":[353],"consisting":[354],"ovoid":[357],"head":[358],"(length":[359,377],"2.44":[360],"\u00b1":[361,366,371,379,522,564,597,604,609,614,619],"0.03":[362,367],"\u00b5m;":[363,368],"width":[364],"2.23":[365],"ratio":[369],"1.11":[370],"0.01),":[372],"single":[375],"flagellum":[376],"30.99":[378],"0.49":[380],"\u00b5m)":[381],"connected":[382],"short":[385],"midpiece.":[386],"Based":[387,579],"these":[389,658],"measurements,":[390],"profile":[393],"created":[395],"Androvision":[398],"CASA":[399,469],"system":[400],"identify":[402,717],"spermatozoa.":[404,623,656],"Accuracy":[405],"count":[410],"correlated":[413,508,545],"method":[418],"(r":[419,452],"=":[420,453,456,459,491],"0.99,":[421,516],"P":[422,455,517,559],"<":[423,518,560],"0.001).":[424,519,561],"Sample":[425],"1:1000":[428],"(across":[429],"5":[430],"dilutions":[431],"ranging":[432],"1:250,":[434],"1:500;":[435],"1:1000,":[436],"1:2500":[437],"1:5000)":[439],"gave":[440],"most":[442],"accurate":[443,664],"concentration":[446,463,505],"when":[447,483,509],"0.87,":[454],"0.001,":[457],"ICC":[458],"0.99).":[460],"Automated":[461],"determined":[464],"three":[466],"more":[468],"fields":[470],"view":[472],"similar":[474,695],"improved":[476],"precision":[477],"(coefficient":[478,488],"variation":[480,490],"\u2264":[481],"8.7%)":[482],"8.0%).":[492],"Moreover,":[493,536],"affected":[498],"motile":[511],"immotile":[513],"cells":[514,532,575],"(r=":[515,557],"Approximately":[520],"99.9":[521],"0.07%":[523],"detected":[528,539,570],"dead":[530],"Hoechst+/PI+":[531],"cytometry.":[535,578],"Hoechst/PI":[541,638],"predicted":[547],"0.98,":[558],"71.9":[563],"4.4%":[565],"damaged":[573,622],"FITC+/PI+":[574],"optimized":[585],"conditions":[586],"above,":[587],"exhibited":[591,690],"15.1":[596],"3.6":[598],"x":[599],"109":[600],"sperm/ml":[601],"concentration,":[602,648],"52.8":[603],"9.6%":[605],"total":[606],"13.1":[608],"4.2%":[610],"progressive":[611],"motility,":[612,649],"64.2":[613],"3.5%":[615],"live,":[616],"43.5":[618],"6.0%":[620],"Discussion":[624],"conclusion:":[626],"Through":[627],"several":[628],"technical":[629],"trials,":[630],"we":[631],"use":[635],"CASA,":[637],"staining,":[639],"reliably":[645],"assessing":[646],"integrity":[653],"Importantly,":[657],"permitted":[660],"rapid":[662],"up":[667],"100":[669],"per":[672],"fish;":[673],"providing":[674],"comprehensive":[676],"semen":[680],"characteristics.":[681],"Preliminary":[682],"obtained":[686],"levels":[691],"cellular":[693],"damage":[694,722],"frozen-thawed":[699],"other":[702],"(Zilli":[704],"2003;":[707],"P\u00e9rez-Cerezales":[708],"2010).":[711],"Further":[712],"research":[713],"necessary":[715],"cause":[719],"whether":[725],"it":[726],"artificially":[728],"induced":[729],"handling":[732],"procedures":[733],"(e.g.":[734],"sub-optimal":[735],"extender,":[736],"prolonged":[737],"storage),":[738],"result":[742],"poor":[744],"some":[748],"individuals":[749],"could":[751],"potentially":[752],"explain":[753],"underlying":[755],"mechanism":[756],"previously":[761],"observed":[762],"offspring":[764],"captive":[766],"bred":[767],"barramundi.":[768]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2019-12-26T00:00:00"}
