{"id":"https://openalex.org/W2995261047","doi":"https://doi.org/10.3389/fmicb.2019.02917","title":"Development of a Gold Nanoparticle-Based Lateral-Flow Immunoassay for Pneumocystis Pneumonia Serological Diagnosis at Point-of-Care","display_name":"Development of a Gold Nanoparticle-Based Lateral-Flow Immunoassay for Pneumocystis Pneumonia Serological Diagnosis at Point-of-Care","publication_year":2019,"publication_date":"2019-12-19","ids":{"openalex":"https://openalex.org/W2995261047","doi":"https://doi.org/10.3389/fmicb.2019.02917","mag":"2995261047","pmid":"https://pubmed.ncbi.nlm.nih.gov/31921081","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6931265"},"language":"en","primary_location":{"id":"doi:10.3389/fmicb.2019.02917","doi":"https://doi.org/10.3389/fmicb.2019.02917","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2019.02917","pdf_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2019.02917/pdf","source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2019.02917/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017749354","display_name":"Ana Lu\u00edsa Tom\u00e1s","orcid":"https://orcid.org/0000-0003-2254-6154","observed_orcids":["https://orcid.org/0000-0003-2254-6154"]},"institutions":[{"id":"https://openalex.org/I83558840","display_name":"Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89","country_code":"PT","type":"education","lineage":["https://openalex.org/I83558840"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Ana Lu\u00edsa Tom\u00e1s","raw_affiliation_strings":["Medical Parasitology Unit, Group of Opportunistic Protozoa/HIV and Other Protozoa, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa, Lisbon, Portugal","UCIBIO, REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Parasitology Unit, Group of Opportunistic Protozoa/HIV and Other Protozoa, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa, Lisbon, Portugal","institution_ids":["https://openalex.org/I83558840"]},{"raw_affiliation_string":"UCIBIO, REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal","institution_ids":["https://openalex.org/I83558840"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020327427","display_name":"Miguel Peixoto de Almeida","orcid":"https://orcid.org/0000-0001-8621-4701","observed_orcids":["https://orcid.org/0000-0001-8621-4701"]},"institutions":[{"id":"https://openalex.org/I182534213","display_name":"Universidade do Porto","ror":"https://ror.org/043pwc612","country_code":"PT","type":"education","lineage":["https://openalex.org/I182534213"]},{"id":"https://openalex.org/I4210138594","display_name":"Rede de Qu\u00edmica e Tecnologia","ror":"https://ror.org/04j14t006","country_code":"PT","type":"nonprofit","lineage":["https://openalex.org/I4210138594"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Miguel P. de Almeida","raw_affiliation_strings":["REQUIMTE/LAQV, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade do Porto, Porto, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"REQUIMTE/LAQV, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade do Porto, Porto, Portugal","institution_ids":["https://openalex.org/I182534213","https://openalex.org/I4210138594"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080812471","display_name":"Fernando Manuel Henriques Cardoso","orcid":"https://orcid.org/0000-0003-1501-9479","observed_orcids":["https://orcid.org/0000-0003-1501-9479"]},"institutions":[{"id":"https://openalex.org/I83558840","display_name":"Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89","country_code":"PT","type":"education","lineage":["https://openalex.org/I83558840"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Fernando Cardoso","raw_affiliation_strings":["Medical Parasitology Unit, Group of Opportunistic Protozoa/HIV and Other Protozoa, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa, Lisbon, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Parasitology Unit, Group of Opportunistic Protozoa/HIV and Other Protozoa, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa, Lisbon, Portugal","institution_ids":["https://openalex.org/I83558840"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066460531","display_name":"Mafalda Pinto","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I83558840","display_name":"Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89","country_code":"PT","type":"education","lineage":["https://openalex.org/I83558840"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Mafalda Pinto","raw_affiliation_strings":["UCIBIO, REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UCIBIO, REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal","institution_ids":["https://openalex.org/I83558840"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051833329","display_name":"Eul\u00e1lia Pereira","orcid":"https://orcid.org/0000-0003-2086-5696","observed_orcids":["https://orcid.org/0000-0003-2086-5696"]},"institutions":[{"id":"https://openalex.org/I182534213","display_name":"Universidade do Porto","ror":"https://ror.org/043pwc612","country_code":"PT","type":"education","lineage":["https://openalex.org/I182534213"]},{"id":"https://openalex.org/I4210138594","display_name":"Rede de Qu\u00edmica e Tecnologia","ror":"https://ror.org/04j14t006","country_code":"PT","type":"nonprofit","lineage":["https://openalex.org/I4210138594"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Eul\u00e1lia Pereira","raw_affiliation_strings":["REQUIMTE/LAQV, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade do Porto, Porto, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"REQUIMTE/LAQV, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade do Porto, Porto, Portugal","institution_ids":["https://openalex.org/I182534213","https://openalex.org/I4210138594"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051551010","display_name":"Ricardo Franco","orcid":"https://orcid.org/0000-0002-5139-2871","observed_orcids":["https://orcid.org/0000-0002-5139-2871"]},"institutions":[{"id":"https://openalex.org/I83558840","display_name":"Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89","country_code":"PT","type":"education","lineage":["https://openalex.org/I83558840"]}],"countries":["PT"],"is_corresponding":true,"raw_author_name":"Ricardo Franco","raw_affiliation_strings":["UCIBIO, REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UCIBIO, REQUIMTE, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal","institution_ids":["https://openalex.org/I83558840"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5079448321","display_name":"Olga Matos","orcid":"https://orcid.org/0000-0001-5793-7716","observed_orcids":["https://orcid.org/0000-0001-5793-7716"]},"institutions":[{"id":"https://openalex.org/I83558840","display_name":"Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89","country_code":"PT","type":"education","lineage":["https://openalex.org/I83558840"]}],"countries":["PT"],"is_corresponding":true,"raw_author_name":"Olga Matos","raw_affiliation_strings":["Medical Parasitology Unit, Group of Opportunistic Protozoa/HIV and Other Protozoa, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa, Lisbon, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Parasitology Unit, Group of Opportunistic Protozoa/HIV and Other Protozoa, Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa, Lisbon, Portugal","institution_ids":["https://openalex.org/I83558840"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5051551010","https://openalex.org/A5079448321"],"corresponding_institution_ids":["https://openalex.org/I83558840"],"apc_list":{"value":2950,"currency":"USD","value_usd":2950},"apc_paid":{"value":2950,"currency":"USD","value_usd":2950},"fwci":2.3461,"has_fulltext":true,"cited_by_count":53,"citation_normalized_percentile":{"value":0.88829415,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"10","issue":null,"first_page":"2917","last_page":"2917"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.8734999895095825,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.8734999895095825,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12369","display_name":"Pneumocystis jirovecii pneumonia detection and treatment","score":0.11969999969005585,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11754","display_name":"SARS-CoV-2 detection and testing","score":0.002300000051036477,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pneumocystis-jirovecii-pneumonia","display_name":"Pneumocystis carinii pneumonia","score":0.9819999933242798},{"id":"https://openalex.org/keywords/lateral-flow-immunoassay","display_name":"lateral flow immunoassay","score":0.8709999918937683},{"id":"https://openalex.org/keywords/gold-nanoparticles","display_name":"gold nanoparticles","score":0.9229999780654907},{"id":"https://openalex.org/keywords/point-of-care-diagnosis","display_name":"point-of-care diagnosis","score":0.8169999718666077},{"id":"https://openalex.org/keywords/serological-diagnosis","display_name":"serological diagnosis","score":0.9369999766349792},{"id":"https://openalex.org/keywords/major-surface-glycoprotein","display_name":"major surface glycoprotein","score":0.9279999732971191},{"id":"https://openalex.org/keywords/igm","display_name":"IgM","score":0.8999999761581421},{"id":"https://openalex.org/keywords/non-invasive-diagnosis","display_name":"non-invasive diagnosis","score":0.6819999814033508},{"id":"https://openalex.org/keywords/hiv-associated-opportunistic-infections","display_name":"HIV-associated opportunistic infections","score":0.6679999828338623},{"id":"https://openalex.org/keywords/low-cost-diagnostics","display_name":"low-cost diagnostics","score":0.42500001192092896}],"concepts":[{"id":"https://openalex.org/C45189115","wikidata":"https://www.wikidata.org/wiki/Q502159","display_name":"Serology","level":3,"score":0.8263115286827087},{"id":"https://openalex.org/C2780420688","wikidata":"https://www.wikidata.org/wiki/Q759183","display_name":"Immunoassay","level":3,"score":0.6306076049804688},{"id":"https://openalex.org/C2777160144","wikidata":"https://www.wikidata.org/wiki/Q15401586","display_name":"Pneumocystis pneumonia","level":4,"score":0.5706909894943237},{"id":"https://openalex.org/C2777914695","wikidata":"https://www.wikidata.org/wiki/Q12192","display_name":"Pneumonia","level":2,"score":0.5706537961959839},{"id":"https://openalex.org/C40993552","wikidata":"https://www.wikidata.org/wiki/Q514654","display_name":"Gold standard (test)","level":2,"score":0.5338296294212341},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.508752167224884},{"id":"https://openalex.org/C51915194","wikidata":"https://www.wikidata.org/wiki/Q17075487","display_name":"Point of care","level":2,"score":0.5015802383422852},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.4978494644165039},{"id":"https://openalex.org/C64841741","wikidata":"https://www.wikidata.org/wiki/Q491983","display_name":"Point-of-care testing","level":2,"score":0.4205617308616638},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3668621778488159},{"id":"https://openalex.org/C177713679","wikidata":"https://www.wikidata.org/wiki/Q679690","display_name":"Intensive care medicine","level":1,"score":0.3251933753490448},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.3112463355064392},{"id":"https://openalex.org/C3013748606","wikidata":"https://www.wikidata.org/wiki/Q15787","display_name":"Human immunodeficiency virus (HIV)","level":2,"score":0.29460209608078003},{"id":"https://openalex.org/C2777559004","wikidata":"https://www.wikidata.org/wiki/Q134478","display_name":"Pneumocystis jirovecii","level":3,"score":0.26611340045928955},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.19948720932006836},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.16947755217552185}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.3389/fmicb.2019.02917","doi":"https://doi.org/10.3389/fmicb.2019.02917","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2019.02917","pdf_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2019.02917/pdf","source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},{"id":"pmid:31921081","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31921081","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6931265","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC6931265/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Microbiol","raw_type":"Text"},{"id":"pmh:oai:run.unl.pt:10362/100877","doi":null,"is_oa":true,"landing_page_url":"https://www.frontiersin.org/article/10.3389/fmicb.2019.02917/full","pdf_url":"http://hdl.handle.net/10362/100877","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"journal article"},{"id":"pmh:oai:doaj.org/article:1780d1087eec4ceba73b90971de2cfbc","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/1780d1087eec4ceba73b90971de2cfbc","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Microbiology, Vol 10 (2019)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fmicb.2019.02917","doi":"https://doi.org/10.3389/fmicb.2019.02917","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fmicb.2019.02917","pdf_url":"https://www.frontiersin.org/articles/10.3389/fmicb.2019.02917/pdf","source":{"id":"https://openalex.org/S2484704948","display_name":"Frontiers in Microbiology","issn_l":"1664-302X","issn":["1664-302X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.98580002784729,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1121218623","display_name":null,"funder_award_id":"007728","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G1480306555","display_name":"UID/BIA/04004 .: Centro de Ecologia Funcional","funder_award_id":"POCI-01-0145-FEDER-007265","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G1516346500","display_name":null,"funder_award_id":"01-0145","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G1630133707","display_name":"Development of an innovative approach for immunonanodiagnosis of Pneumocystis pneumonia at the point-of-care.","funder_award_id":"SFRH/BD/108433/2015","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G1763632427","display_name":null,"funder_award_id":"POCI-01-0145","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G1767590278","display_name":null,"funder_award_id":"POCI/01/0145/FEDER/007265","funder_id":"https://openalex.org/F4320323642","funder_display_name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior"},{"id":"https://openalex.org/G274581676","display_name":null,"funder_award_id":"POCI-01","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G2860021946","display_name":null,"funder_award_id":"UID/Multi/04378/2019","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G299573334","display_name":null,"funder_award_id":"POCI/01/0145/FEDER/007265","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G3249272234","display_name":null,"funder_award_id":"01-0145-FEDER-007265","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G3574862991","display_name":null,"funder_award_id":"PGG/001/2014","funder_id":"https://openalex.org/F4320310815","funder_display_name":"Gilead Foundation"},{"id":"https://openalex.org/G3697640993","display_name":null,"funder_award_id":"UID/QUI/50006/2019","funder_id":"https://openalex.org/F4320330089","funder_display_name":"Rede de Qu\u00edmica e Tecnologia"},{"id":"https://openalex.org/G3840324753","display_name":null,"funder_award_id":"POCI-01-0145-FEDER-","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G384318868","display_name":null,"funder_award_id":"POCI/01/0145/FEDER/007728","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G3902363903","display_name":null,"funder_award_id":"POCI/01/0145/FEDER/007728","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G3993711494","display_name":null,"funder_award_id":"UID/QUI/50006/2019","funder_id":"https://openalex.org/F4320330090","funder_display_name":"Laborat\u00f3rio Associado para a Qu\u00edmica Verde"},{"id":"https://openalex.org/G4067848518","display_name":null,"funder_award_id":"UID/QUI/50006/2019","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G4135528483","display_name":"Sa\u00fade Global e Medicina Tropical","funder_award_id":"UID/Multi/04413/2013","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G4393367909","display_name":null,"funder_award_id":"SFRH/BD/95983/2013","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G4506485401","display_name":"\"Bionanoconjugados de nanopart\u00edculas de ouro e de prata para biossensores.\"","funder_award_id":"SFRH/BD/95983/2013","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G4724859719","display_name":null,"funder_award_id":"01-0145","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G5016002722","display_name":null,"funder_award_id":"UID/ QUI/ 50006/ 2019","funder_id":"https://openalex.org/F4320323642","funder_display_name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior"},{"id":"https://openalex.org/G5224927334","display_name":null,"funder_award_id":"POCI-01-","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G5261853715","display_name":"UID/EMS/00151 .: Centro da Ci\u00eancias e Tecnologias Mec\u00e2nicas e Aeroespaciais","funder_award_id":"POCI-01-0145-FEDER-007728","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G5281204143","display_name":null,"funder_award_id":"UID/MULTI/04378/2019","funder_id":"https://openalex.org/F4320329036","funder_display_name":"Applied Molecular Biosciences Unit"},{"id":"https://openalex.org/G5491772409","display_name":null,"funder_award_id":"UID/QUI/50006/2019","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G5517610464","display_name":null,"funder_award_id":"UID/Multi/04378/2019","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G5677667711","display_name":null,"funder_award_id":"POCI-01-0145","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G6193589897","display_name":null,"funder_award_id":"POCI-01-0145","funder_id":"https://openalex.org/F4320323642","funder_display_name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior"},{"id":"https://openalex.org/G6703874277","display_name":null,"funder_award_id":"UID/Multi /04378/2019","funder_id":"https://openalex.org/F4320323642","funder_display_name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior"},{"id":"https://openalex.org/G6837649865","display_name":null,"funder_award_id":"POCI/01/0145/FEDER/007728","funder_id":"https://openalex.org/F4320329036","funder_display_name":"Applied Molecular Biosciences Unit"},{"id":"https://openalex.org/G6967460350","display_name":"Laborat\u00f3rio Associado para a Qu\u00edmica Verde - Tecnologias e Processos Limpos","funder_award_id":"UID/QUI/50006/2019","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G716833851","display_name":null,"funder_award_id":"UID/QUI/50006","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G7965899483","display_name":null,"funder_award_id":"POCI-01-0145- FEDER","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"},{"id":"https://openalex.org/G7972280800","display_name":null,"funder_award_id":"UID/QUI/50006/2019","funder_id":"https://openalex.org/F4320335059","funder_display_name":"Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa"},{"id":"https://openalex.org/G804512570","display_name":null,"funder_award_id":"POCI-01-0145-FEDER-007265","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G8075074916","display_name":"Unidade de Ci\u00eancias Biomoleculares Aplicadas","funder_award_id":"UID/Multi/04378/2019","funder_id":"https://openalex.org/F4320334779","funder_display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia"},{"id":"https://openalex.org/G8938862326","display_name":null,"funder_award_id":"POCI-01-0145-FEDER-007728","funder_id":"https://openalex.org/F4320323642","funder_display_name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior"},{"id":"https://openalex.org/G977537520","display_name":null,"funder_award_id":"007265","funder_id":"https://openalex.org/F4320335322","funder_display_name":"European Regional Development Fund"}],"funders":[{"id":"https://openalex.org/F4320308573","display_name":"Gilead Sciences","ror":"https://ror.org/056546b03"},{"id":"https://openalex.org/F4320310815","display_name":"Gilead Foundation","ror":"https://ror.org/056546b03"},{"id":"https://openalex.org/F4320320300","display_name":"European Commission","ror":"https://ror.org/00k4n6c32"},{"id":"https://openalex.org/F4320323459","display_name":"Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89"},{"id":"https://openalex.org/F4320323642","display_name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Ensino Superior","ror":"https://ror.org/045b9pr88"},{"id":"https://openalex.org/F4320329036","display_name":"Applied Molecular Biosciences Unit","ror":"https://ror.org/04c3k8v21"},{"id":"https://openalex.org/F4320330089","display_name":"Rede de Qu\u00edmica e Tecnologia","ror":"https://ror.org/04j14t006"},{"id":"https://openalex.org/F4320330090","display_name":"Laborat\u00f3rio Associado para a Qu\u00edmica Verde","ror":"https://ror.org/047td8p12"},{"id":"https://openalex.org/F4320331406","display_name":"NOVA Laboratory for Computer Science and Informatics","ror":null},{"id":"https://openalex.org/F4320334779","display_name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","ror":"https://ror.org/00snfqn58"},{"id":"https://openalex.org/F4320335059","display_name":"Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa","ror":"https://ror.org/02xankh89"},{"id":"https://openalex.org/F4320335322","display_name":"European Regional Development Fund","ror":"https://ror.org/00k4n6c32"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2995261047.pdf","grobid_xml":"https://content.openalex.org/works/W2995261047.grobid-xml"},"referenced_works_count":57,"referenced_works":["https://openalex.org/W85863287","https://openalex.org/W128205058","https://openalex.org/W200739673","https://openalex.org/W311876177","https://openalex.org/W1720039819","https://openalex.org/W1828922723","https://openalex.org/W1968696159","https://openalex.org/W1969260462","https://openalex.org/W1976491190","https://openalex.org/W1977559519","https://openalex.org/W1984567143","https://openalex.org/W1989211576","https://openalex.org/W1997161044","https://openalex.org/W2004059993","https://openalex.org/W2010582892","https://openalex.org/W2014468482","https://openalex.org/W2014769662","https://openalex.org/W2021194998","https://openalex.org/W2021942763","https://openalex.org/W2025645042","https://openalex.org/W2027573369","https://openalex.org/W2029856902","https://openalex.org/W2036199624","https://openalex.org/W2044440155","https://openalex.org/W2072328720","https://openalex.org/W2076348126","https://openalex.org/W2086687107","https://openalex.org/W2103135951","https://openalex.org/W2106512916","https://openalex.org/W2116374839","https://openalex.org/W2119615572","https://openalex.org/W2127094219","https://openalex.org/W2134369375","https://openalex.org/W2134515864","https://openalex.org/W2138471314","https://openalex.org/W2146011528","https://openalex.org/W2146702713","https://openalex.org/W2149473208","https://openalex.org/W2151217507","https://openalex.org/W2155954788","https://openalex.org/W2156308226","https://openalex.org/W2161964466","https://openalex.org/W2164071029","https://openalex.org/W2174347897","https://openalex.org/W2256765887","https://openalex.org/W2384346244","https://openalex.org/W2528567646","https://openalex.org/W2535710483","https://openalex.org/W2553589539","https://openalex.org/W2556061900","https://openalex.org/W2571210767","https://openalex.org/W2765328149","https://openalex.org/W2784930601","https://openalex.org/W2806349086","https://openalex.org/W2900752871","https://openalex.org/W2913959393","https://openalex.org/W4244469832"],"related_works":["https://openalex.org/W2947553618","https://openalex.org/W3094017917","https://openalex.org/W2553589539","https://openalex.org/W7112125857","https://openalex.org/W4392892898","https://openalex.org/W2010582892","https://openalex.org/W2307721566","https://openalex.org/W2900752871","https://openalex.org/W3024628859","https://openalex.org/W2565809807"],"abstract_inverted_index":{"Pneumocystis":[0],"jirovecii":[1,104,126,140,161,228],"pneumonia":[2],"(PcP)":[3],"is":[4],"a":[5,51,63,262,275],"major":[6,76],"human":[7,164],"immunodeficiency":[8],"virus":[9],"(HIV)-related":[10],"illness,":[11],"rising":[12],"among":[13],"immunocompromised":[14],"non-HIV":[15],"patients":[16,40,112,132,137,243],"and":[17,30,55,80,87,107,116,195,215,236,247,264,273,300,316,324],"in":[18,38,43,96,109,131,163,191,322],"developing":[19],"countries.":[20],"Presently,":[21],"the":[22,48,155,170,198,255,259,270,281,290,310,330,334],"diagnosis":[23,315],"requires":[24],"respiratory":[25],"specimens":[26],"obtained":[27],"through":[28],"invasive":[29],"costly":[31],"techniques":[32],"that":[33,122],"are":[34],"difficult":[35],"to":[36,58,217,221,308,328],"perform":[37],"all":[39,44,332],"or":[41,202],"implement":[42],"economic":[45],"settings.":[46],"Therefore,":[47],"development":[49],"of":[50,73,101,111,157,173,240,261,292,302,313,319],"faster,":[52],"cost-effective,":[53],"non-invasive":[54],"field-friendly":[56],"test":[57,263],"diagnose":[59],"PcP":[60,134,293,320],"would":[61],"be":[62],"significant":[64],"advance.":[65],"In":[66],"this":[67,303],"study,":[68],"recombinant":[69],"synthetic":[70],"antigens":[71],"(RSA)":[72],"P.":[74,139,174],"jirovecii\u2019s":[75,175],"surface":[77],"glycoprotein":[78],"(Msg)":[79],"kexin-like":[81],"serine":[82],"protease":[83],"(Kex1)":[84],"were":[85,91,128,167,193,208,234],"produced":[86],"purified.":[88],"These":[89,205,284],"RSA":[90,176,200],"applied":[92],"as":[93],"antigenic":[94],"tools":[95],"immunoenzymatic":[97],"assays":[98],"for":[99],"detection":[100,156],"specific":[102,158],"anti-P.":[103,125,160,227],"antibodies":[105,162],"(IgG":[106],"IgM)":[108],"sera":[110,165,241],"with":[113,133,136,143,177,187,197,224,238,244,269,280],"(n":[114],"=48)":[115],"without":[117,138,248],"(n=28)":[118],"PcP.":[119,251],"Results":[120],"showed":[121],"only":[123,274],"IgM":[124,159,226],"levels":[127],"significantly":[129],"increased":[130],"compared":[135],"infection":[141],"(p\u22640.001":[142],"both":[144,321],"RSA).":[145],"Thus,":[146],"two":[147,199,231],"strip":[148],"lateral":[149],"flow":[150],"immunoassays":[151],"(LFIA),":[152],"based":[153],"on":[154],"samples,":[166],"developed":[168,235],"using":[169],"innovative":[171],"association":[172],"spherical":[178,185],"gold":[179],"nanoparticles":[180],"(AuNPs).":[181],"For":[182],"that,":[183],"alkanethiol-functionalized":[184],"AuNPs":[186],"ca.":[188],"\u224840":[189],"nm":[190],"diameter":[192],"synthetized":[194],"conjugated":[196],"(Msg":[201],"Kex1)":[203],"produced.":[204],"AuNP-RSA":[206],"conjugates":[207],"characterized":[209],"by":[210],"agarose":[211],"gel":[212],"electrophoresis":[213],"(AGE)":[214],"optimized":[216],"improve":[218],"their":[219],"ability":[220],"interact":[222],"specifically":[223],"serum":[225],"antibodies.":[229],"Finally,":[230],"LFIA":[232,253],"prototypes":[233],"tested":[237],"pools":[239],"from":[242],"(positive":[245],"sample)":[246,250],"(negative":[249],"Both":[252],"had":[254],"expected":[256],"performance,":[257],"namely,":[258],"presence":[260],"control":[265,276],"red":[266,277],"colored":[267,278],"lines":[268],"positive":[271],"sample,":[272],"line":[279],"negative":[282],"sample.":[283],"results":[285],"provide":[286],"valuable":[287],"insights":[288],"into":[289],"possibility":[291],"serodiagnosis":[294],"at":[295],"point-of-care.":[296],"The":[297],"optimization,":[298],"validation":[299],"implementation":[301],"strip-based":[304],"approach":[305],"may":[306],"help":[307],"reduce":[309],"high":[311],"cost":[312],"medical":[314],"subsequent":[317],"treatment":[318],"industrialized":[323],"low-income":[325],"regions,":[326],"helping":[327],"manage":[329],"disease":[331],"around":[333],"world.":[335]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":8},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":11},{"year":2021,"cited_by_count":12},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":1}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2019-12-26T00:00:00"}