{"id":"https://openalex.org/W2995146393","doi":"https://doi.org/10.3390/ani10010020","title":"Isolation of Mycobacterium avium Subsp. Paratuberculosis in the Feces and Tissue of Small Ruminants Using a Non-Automated Liquid Culture Method","display_name":"Isolation of Mycobacterium avium Subsp. Paratuberculosis in the Feces and Tissue of Small Ruminants Using a Non-Automated Liquid Culture Method","publication_year":2019,"publication_date":"2019-12-20","ids":{"openalex":"https://openalex.org/W2995146393","doi":"https://doi.org/10.3390/ani10010020","mag":"2995146393","pmid":"https://pubmed.ncbi.nlm.nih.gov/31861845","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7023119"},"language":"en","primary_location":{"id":"doi:10.3390/ani10010020","doi":"https://doi.org/10.3390/ani10010020","is_oa":true,"landing_page_url":"http://dx.doi.org/10.3390/ani10010020","pdf_url":"https://www.mdpi.com/2076-2615/10/1/20/pdf?version=1576836311","source":{"id":"https://openalex.org/S4210168508","display_name":"Animals","issn_l":"2076-2615","issn":["2076-2615"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Animals","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/2076-2615/10/1/20/pdf?version=1576836311","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111407309","display_name":"Luigi De Grossi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210150891","display_name":"Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana","ror":"https://ror.org/05pfcz666","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210150891"]}],"countries":["IT"],"is_corresponding":true,"raw_author_name":"Luigi De Grossi","raw_affiliation_strings":["Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Roma, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Roma, Italy","institution_ids":["https://openalex.org/I4210150891"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088645906","display_name":"Davide Santori","orcid":"https://orcid.org/0009-0005-3112-5951","observed_orcids":["https://orcid.org/0009-0005-3112-5951"]},"institutions":[{"id":"https://openalex.org/I4210150891","display_name":"Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana","ror":"https://ror.org/05pfcz666","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210150891"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Davide Santori","raw_affiliation_strings":["Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Roma, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Roma, Italy","institution_ids":["https://openalex.org/I4210150891"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110415187","display_name":"Antonino Barone","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210150891","display_name":"Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana","ror":"https://ror.org/05pfcz666","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210150891"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Antonino Barone","raw_affiliation_strings":["Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Roma, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Roma, Italy","institution_ids":["https://openalex.org/I4210150891"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065647992","display_name":"Silvia Abbruzzese","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210150891","display_name":"Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana","ror":"https://ror.org/05pfcz666","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210150891"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Silvia Abbruzzese","raw_affiliation_strings":["Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Roma, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Roma, Italy","institution_ids":["https://openalex.org/I4210150891"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026160772","display_name":"Matteo Ricchi","orcid":"https://orcid.org/0000-0002-4923-0216","observed_orcids":["https://orcid.org/0000-0002-4923-0216"]},"institutions":[{"id":"https://openalex.org/I4210116218","display_name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \"Bruno Ubertini\"","ror":"https://ror.org/02qcq7v36","country_code":"IT","type":"other","lineage":["https://openalex.org/I4210116218"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Matteo Ricchi","raw_affiliation_strings":["Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna B. Ubertini, 25124 Brescia, Italy"],"raw_orcid":"https://orcid.org/0000-0002-4923-0216","affiliations":[{"raw_affiliation_string":"Istituto Zooprofilattico Sperimentale della Lombardia e dell\u2019Emilia Romagna B. Ubertini, 25124 Brescia, Italy","institution_ids":["https://openalex.org/I4210116218"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039571799","display_name":"Gaetana Anita Marcario","orcid":"https://orcid.org/0009-0000-4602-9317","observed_orcids":["https://orcid.org/0009-0000-4602-9317"]},"institutions":[{"id":"https://openalex.org/I4210150891","display_name":"Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana","ror":"https://ror.org/05pfcz666","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210150891"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Gaetana Anita Marcario","raw_affiliation_strings":["Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Roma, Italy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, 00178 Roma, Italy","institution_ids":["https://openalex.org/I4210150891"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5111407309"],"corresponding_institution_ids":["https://openalex.org/I4210150891"],"apc_list":{"value":1200,"currency":"CHF","value_usd":1216},"apc_paid":{"value":1200,"currency":"CHF","value_usd":1216},"fwci":0.758,"has_fulltext":true,"cited_by_count":8,"citation_normalized_percentile":{"value":0.68660714,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"10","issue":"1","first_page":"20","last_page":"20"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11316","display_name":"Mycobacterium research and diagnosis","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11316","display_name":"Mycobacterium research and diagnosis","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10038","display_name":"Tuberculosis Research and Epidemiology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/paratuberculosis","display_name":"paratuberculosis","score":0.9789999723434448},{"id":"https://openalex.org/keywords/mycobacterium-avium-subsp-paratuberculosis","display_name":"Mycobacterium avium subsp. paratuberculosis","score":0.9890000224113464},{"id":"https://openalex.org/keywords/small-ruminants","display_name":"small ruminants","score":0.9120000004768372},{"id":"https://openalex.org/keywords/liquid-culture","display_name":"liquid culture","score":0.6759999990463257},{"id":"https://openalex.org/keywords/fecal-shedding","display_name":"fecal shedding","score":0.6200000047683716},{"id":"https://openalex.org/keywords/serological-screening","display_name":"serological screening","score":0.5680000185966492},{"id":"https://openalex.org/keywords/bacterial-isolation","display_name":"bacterial isolation","score":0.5440000295639038}],"concepts":[{"id":"https://openalex.org/C2777849792","wikidata":"https://www.wikidata.org/wiki/Q707388","display_name":"Paratuberculosis","level":4,"score":0.9443783760070801},{"id":"https://openalex.org/C61716771","wikidata":"https://www.wikidata.org/wiki/Q496","display_name":"Feces","level":2,"score":0.7966492772102356},{"id":"https://openalex.org/C2775941552","wikidata":"https://www.wikidata.org/wiki/Q25212305","display_name":"Isolation (microbiology)","level":2,"score":0.7383683323860168},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.7240438461303711},{"id":"https://openalex.org/C2909019056","wikidata":"https://www.wikidata.org/wiki/Q6946990","display_name":"Mycobacterium avium subsp. paratuberculosis","level":5,"score":0.6874783635139465},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6588501930236816},{"id":"https://openalex.org/C134215735","wikidata":"https://www.wikidata.org/wiki/Q120997","display_name":"Flock","level":2,"score":0.5558256506919861},{"id":"https://openalex.org/C2781209916","wikidata":"https://www.wikidata.org/wiki/Q3215756","display_name":"Typing","level":2,"score":0.5536299347877502},{"id":"https://openalex.org/C2780374374","wikidata":"https://www.wikidata.org/wiki/Q194309","display_name":"Mycobacterium","level":3,"score":0.5282078385353088},{"id":"https://openalex.org/C180032290","wikidata":"https://www.wikidata.org/wiki/Q1664296","display_name":"Inoculation","level":2,"score":0.46390458941459656},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3922654390335083},{"id":"https://openalex.org/C42972112","wikidata":"https://www.wikidata.org/wiki/Q170201","display_name":"Veterinary medicine","level":1,"score":0.32870280742645264},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.3034670948982239},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1130247414112091},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10933715105056763},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3390/ani10010020","doi":"https://doi.org/10.3390/ani10010020","is_oa":true,"landing_page_url":"http://dx.doi.org/10.3390/ani10010020","pdf_url":"https://www.mdpi.com/2076-2615/10/1/20/pdf?version=1576836311","source":{"id":"https://openalex.org/S4210168508","display_name":"Animals","issn_l":"2076-2615","issn":["2076-2615"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Animals","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:6009168","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7023119","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:31861845","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31861845","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Animals : an open access journal from MDPI","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7023119","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC7023119/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Animals (Basel)","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.3390/ani10010020","doi":"https://doi.org/10.3390/ani10010020","is_oa":true,"landing_page_url":"http://dx.doi.org/10.3390/ani10010020","pdf_url":"https://www.mdpi.com/2076-2615/10/1/20/pdf?version=1576836311","source":{"id":"https://openalex.org/S4210168508","display_name":"Animals","issn_l":"2076-2615","issn":["2076-2615"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Animals","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"},{"score":0.5666999816894531,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2995146393.pdf","grobid_xml":"https://content.openalex.org/works/W2995146393.grobid-xml"},"referenced_works_count":45,"referenced_works":["https://openalex.org/W29392634","https://openalex.org/W171158026","https://openalex.org/W225253954","https://openalex.org/W1486306476","https://openalex.org/W1502698740","https://openalex.org/W1694868649","https://openalex.org/W1979434663","https://openalex.org/W1988286531","https://openalex.org/W1988590384","https://openalex.org/W1990074879","https://openalex.org/W1991454289","https://openalex.org/W1995309889","https://openalex.org/W2000175523","https://openalex.org/W2000457359","https://openalex.org/W2007732778","https://openalex.org/W2009265889","https://openalex.org/W2015449898","https://openalex.org/W2019742321","https://openalex.org/W2023510719","https://openalex.org/W2025724169","https://openalex.org/W2046497873","https://openalex.org/W2053156420","https://openalex.org/W2057861161","https://openalex.org/W2062093964","https://openalex.org/W2066954163","https://openalex.org/W2079354359","https://openalex.org/W2088185802","https://openalex.org/W2095081202","https://openalex.org/W2115068691","https://openalex.org/W2116190810","https://openalex.org/W2119075534","https://openalex.org/W2131691226","https://openalex.org/W2137265604","https://openalex.org/W2139373916","https://openalex.org/W2142897686","https://openalex.org/W2144173763","https://openalex.org/W2144241830","https://openalex.org/W2156091261","https://openalex.org/W2158680478","https://openalex.org/W2158716987","https://openalex.org/W2162273223","https://openalex.org/W2164616601","https://openalex.org/W2280170386","https://openalex.org/W2331940910","https://openalex.org/W2801054967"],"related_works":["https://openalex.org/W4392342609","https://openalex.org/W2291766734","https://openalex.org/W2763921523","https://openalex.org/W1965174100","https://openalex.org/W4390882149","https://openalex.org/W2599570137","https://openalex.org/W2613050994","https://openalex.org/W3011854713","https://openalex.org/W4225313289","https://openalex.org/W7216213560"],"abstract_inverted_index":{"Paratuberculosis":[0,12],"is":[1,21],"a":[2,63],"chronic":[3],"disease":[4],"of":[5,16,41,99,142,160,169,191],"ruminants":[6],"caused":[7],"by":[8,32,109,115,135,154],"Mycobacterium":[9],"avium":[10],"subsp.":[11],"(MAP).":[13],"Since":[14],"isolation":[15,141],"MAP":[17,42,100,123,146,170],"type":[18,40,144,168,192],"I":[19,145,193],"(S)":[20],"rarely":[22],"reported":[23],"in":[24,72,85,102,118,139,174,183,196],"Italy,":[25,175],"our":[26],"research":[27],"was":[28,92],"aimed":[29],"at":[30],"isolating,":[31],"an":[33,48],"inexpensive":[34],"liquid":[35,88,103,162],"culture":[36,89,136],"manual":[37],"method,":[38],"this":[39,161,167],"isolates.":[43,148],"At":[44],"first,":[45],"we":[46,61],"used":[47,62,94],"ELISA":[49,69],"to":[50,53,74,95,113,120,185],"point":[51],"out":[52,182],"serologically":[54],"positive":[55,70,81],"samples":[56,82,126],"from":[57,79],"five":[58],"flocks.":[59],"Secondly,":[60],"fecal":[64,128],"direct":[65],"IS900-qPCR":[66,80,91],"on":[67],"the":[68,97,122,140,158,187],"samples,":[71],"order":[73,119,184],"detect":[75],"shedder":[76],"animals.":[77],"Feces":[78],"were":[83,106,133,152],"inoculated":[84],"solid":[86,155],"and":[87,111,129,176,189],"media.":[90],"further":[93,107,177],"test":[96],"growth":[98],"isolates":[101,151],"medium,":[104],"which":[105],"confirmed":[108],"f57-qPCR":[110],"submitted":[112],"typing":[114],"specific":[116],"PCR":[117],"identify":[121],"type.":[124],"Twenty-eight":[125],"(24":[127],"four":[130],"tissutal":[131],"samples)":[132],"processed":[134],"methods,":[137],"resulting":[138],"six":[143],"field":[147],"Notably,":[149],"no":[150],"recovered":[153],"media,":[156],"underlining":[157],"utility":[159],"method.":[163],"Few":[164],"data":[165],"about":[166],"are":[171],"currently":[172],"available":[173],"analyses":[178],"should":[179],"be":[180],"carried":[181],"study":[186],"origin":[188],"epidemiology":[190],"strains":[194],"circulating":[195],"Italy.":[197]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2019-12-26T00:00:00"}