{"id":"https://openalex.org/W2992392691","doi":"https://doi.org/10.1073/pnas.1913616116","title":"Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system","display_name":"Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system","publication_year":2019,"publication_date":"2019-12-02","ids":{"openalex":"https://openalex.org/W2992392691","doi":"https://doi.org/10.1073/pnas.1913616116","mag":"2992392691","pmid":"https://pubmed.ncbi.nlm.nih.gov/31792180","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6936394"},"language":"en","primary_location":{"id":"doi:10.1073/pnas.1913616116","doi":"https://doi.org/10.1073/pnas.1913616116","is_oa":true,"landing_page_url":"https://doi.org/10.1073/pnas.1913616116","pdf_url":"https://www.pnas.org/content/pnas/116/52/26491.full.pdf","source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://www.pnas.org/content/pnas/116/52/26491.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5064811632","display_name":"Carola Gregor","orcid":"https://orcid.org/0000-0002-1083-9892","observed_orcids":["https://orcid.org/0000-0002-1083-9892"]},"institutions":[{"id":"https://openalex.org/I4210131661","display_name":"Max Planck Institute for Biophysical Chemistry","ror":"https://ror.org/03e76ya46","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210131661","https://openalex.org/I4387154714"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Carola Gregor","raw_affiliation_strings":["Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;"],"raw_orcid":"https://orcid.org/0000-0002-1083-9892","affiliations":[{"raw_affiliation_string":"Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;","institution_ids":["https://openalex.org/I4210131661"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069726102","display_name":"Jasmin K. Pape","orcid":"https://orcid.org/0000-0003-4403-4520","observed_orcids":["https://orcid.org/0000-0003-4403-4520"]},"institutions":[{"id":"https://openalex.org/I4210131661","display_name":"Max Planck Institute for Biophysical Chemistry","ror":"https://ror.org/03e76ya46","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210131661","https://openalex.org/I4387154714"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Jasmin K. Pape","raw_affiliation_strings":["Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;"],"raw_orcid":"https://orcid.org/0000-0003-4403-4520","affiliations":[{"raw_affiliation_string":"Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;","institution_ids":["https://openalex.org/I4210131661"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051765013","display_name":"Klaus C. Gwosch","orcid":"https://orcid.org/0000-0002-8897-2335","observed_orcids":["https://orcid.org/0000-0002-8897-2335"]},"institutions":[{"id":"https://openalex.org/I4210131661","display_name":"Max Planck Institute for Biophysical Chemistry","ror":"https://ror.org/03e76ya46","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210131661","https://openalex.org/I4387154714"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Klaus C. Gwosch","raw_affiliation_strings":["Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;"],"raw_orcid":"https://orcid.org/0000-0002-8897-2335","affiliations":[{"raw_affiliation_string":"Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;","institution_ids":["https://openalex.org/I4210131661"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079066625","display_name":"Tanja Gilat","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210131661","display_name":"Max Planck Institute for Biophysical Chemistry","ror":"https://ror.org/03e76ya46","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210131661","https://openalex.org/I4387154714"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Tanja Gilat","raw_affiliation_strings":["Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;","institution_ids":["https://openalex.org/I4210131661"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066941336","display_name":"Steffen J. Sahl","orcid":"https://orcid.org/0000-0003-2917-0778","observed_orcids":["https://orcid.org/0000-0003-2917-0778"]},"institutions":[{"id":"https://openalex.org/I4210131661","display_name":"Max Planck Institute for Biophysical Chemistry","ror":"https://ror.org/03e76ya46","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210131661","https://openalex.org/I4387154714"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Steffen J. Sahl","raw_affiliation_strings":["Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;"],"raw_orcid":"https://orcid.org/0000-0003-2917-0778","affiliations":[{"raw_affiliation_string":"Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;","institution_ids":["https://openalex.org/I4210131661"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088219208","display_name":"Stefan W. Hell","orcid":"https://orcid.org/0000-0002-9638-5077","observed_orcids":["https://orcid.org/0000-0002-9638-5077"]},"institutions":[{"id":"https://openalex.org/I4210087042","display_name":"Max Planck Institute for Medical Research","ror":"https://ror.org/000bxzc63","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210087042"]},{"id":"https://openalex.org/I4210131661","display_name":"Max Planck Institute for Biophysical Chemistry","ror":"https://ror.org/03e76ya46","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210131661","https://openalex.org/I4387154714"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Stefan W. Hell","raw_affiliation_strings":["Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;","Department of Optical Nanoscopy, Max Planck Institute for Medical Research, 69120 Heidelberg, Germany"],"raw_orcid":"https://orcid.org/0000-0002-9638-5077","affiliations":[{"raw_affiliation_string":"Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, 37077 G\u00f6ttingen, Germany;","institution_ids":["https://openalex.org/I4210131661"]},{"raw_affiliation_string":"Department of Optical Nanoscopy, Max Planck Institute for Medical Research, 69120 Heidelberg, Germany","institution_ids":["https://openalex.org/I4210087042"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5064811632","https://openalex.org/A5088219208"],"corresponding_institution_ids":["https://openalex.org/I4210087042","https://openalex.org/I4210131661"],"apc_list":null,"apc_paid":null,"fwci":2.8567,"has_fulltext":true,"cited_by_count":73,"citation_normalized_percentile":{"value":0.92657416,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":95,"max":100},"biblio":{"volume":"116","issue":"52","first_page":"26491","last_page":"26496"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10097","display_name":"Luminescence Properties of Advanced Materials","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacterial-bioluminescence","display_name":"bacterial bioluminescence","score":0.8600000143051147},{"id":"https://openalex.org/keywords/single-cell-imaging","display_name":"single-cell imaging","score":0.8650000095367432},{"id":"https://openalex.org/keywords/codon-optimization","display_name":"codon optimization","score":0.7400000095367432}],"concepts":[{"id":"https://openalex.org/C5176359","wikidata":"https://www.wikidata.org/wiki/Q179924","display_name":"Bioluminescence","level":2,"score":0.9855068922042847},{"id":"https://openalex.org/C111335760","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferase","level":4,"score":0.8571851253509521},{"id":"https://openalex.org/C2781309008","wikidata":"https://www.wikidata.org/wiki/Q369745","display_name":"Luciferin","level":5,"score":0.7882554531097412},{"id":"https://openalex.org/C161997846","wikidata":"https://www.wikidata.org/wiki/Q4915066","display_name":"Bioluminescence imaging","level":5,"score":0.7649567723274231},{"id":"https://openalex.org/C173764188","wikidata":"https://www.wikidata.org/wiki/Q4915238","display_name":"Bioreporter","level":5,"score":0.7629527449607849},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5746691226959229},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4857966899871826},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.44156602025032043},{"id":"https://openalex.org/C107477482","wikidata":"https://www.wikidata.org/wiki/Q22570039","display_name":"Firefly protocol","level":2,"score":0.42736661434173584},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.24693253636360168},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.2326636016368866},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.1678902506828308},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.14422142505645752},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.08195233345031738},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.06525066494941711}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.1073/pnas.1913616116","doi":"https://doi.org/10.1073/pnas.1913616116","is_oa":true,"landing_page_url":"https://doi.org/10.1073/pnas.1913616116","pdf_url":"https://www.pnas.org/content/pnas/116/52/26491.full.pdf","source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},{"id":"doi:10.1101/798108","doi":"https://doi.org/10.1101/798108","is_oa":true,"landing_page_url":"https://doi.org/10.1101/798108","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2019/10/08/798108.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:31792180","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31792180","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6936394","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC6936394/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proc Natl Acad Sci U S A","raw_type":"Text"},{"id":"pmh:oai:pure.mpg.de:item_3180532","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/21.11116/0000-0005-4F0F-4","pdf_url":null,"source":{"id":"https://openalex.org/S4306400654","display_name":"MPG.PuRe (Max Planck Society)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I149899117","host_organization_name":"Max Planck Society","host_organization_lineage":["https://openalex.org/I149899117"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Proceedings of the National Academy of Sciences of the United States of America","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1073/pnas.1913616116","doi":"https://doi.org/10.1073/pnas.1913616116","is_oa":true,"landing_page_url":"https://doi.org/10.1073/pnas.1913616116","pdf_url":"https://www.pnas.org/content/pnas/116/52/26491.full.pdf","source":{"id":"https://openalex.org/S125754415","display_name":"Proceedings of the National Academy of Sciences","issn_l":"0027-8424","issn":["0027-8424","1091-6490"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","erih-plus","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320052","host_organization_name":"National Academy of Sciences","host_organization_lineage":["https://openalex.org/P4310320052"],"host_organization_lineage_names":["National Academy of Sciences"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proceedings of the National Academy of Sciences","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321657","display_name":"East China University of Science and Technology","ror":"https://ror.org/01vyrm377"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2992392691.pdf","grobid_xml":"https://content.openalex.org/works/W2992392691.grobid-xml"},"referenced_works_count":32,"referenced_works":["https://openalex.org/W64193448","https://openalex.org/W168342135","https://openalex.org/W1509799647","https://openalex.org/W1542321578","https://openalex.org/W2013815211","https://openalex.org/W2018151200","https://openalex.org/W2031785533","https://openalex.org/W2037150792","https://openalex.org/W2056817309","https://openalex.org/W2064005757","https://openalex.org/W2068408712","https://openalex.org/W2092164018","https://openalex.org/W2102120160","https://openalex.org/W2114214386","https://openalex.org/W2118857917","https://openalex.org/W2126864626","https://openalex.org/W2141554815","https://openalex.org/W2155578830","https://openalex.org/W2157753854","https://openalex.org/W2159532936","https://openalex.org/W2163294864","https://openalex.org/W2167441795","https://openalex.org/W2168036974","https://openalex.org/W2283014055","https://openalex.org/W2568259763","https://openalex.org/W2623012019","https://openalex.org/W2783286406","https://openalex.org/W2790410224","https://openalex.org/W2901596277","https://openalex.org/W2902393547","https://openalex.org/W2909474655","https://openalex.org/W4249406446"],"related_works":["https://openalex.org/W4285251588","https://openalex.org/W4412124393","https://openalex.org/W4403809496","https://openalex.org/W2155578830","https://openalex.org/W2008373600","https://openalex.org/W4415320929","https://openalex.org/W2996489228","https://openalex.org/W2900714201","https://openalex.org/W4403052299","https://openalex.org/W3004937516"],"abstract_inverted_index":{"Significance":[0],"Bioluminescence":[1],"is":[2,46],"generated":[3],"by":[4,99],"luciferases":[5],"that":[6,50],"oxidize":[7],"a":[8],"specific":[9],"luciferin.":[10],"The":[11],"enzymes":[12,107],"involved":[13],"in":[14,62,78],"the":[15,18,42,54,67,74,102,105,109],"synthesis":[16],"of":[17,35,53,95,101],"luciferin":[19],"from":[20,108],"widespread":[21],"cellular":[22],"metabolites":[23],"have":[24],"so":[25,81],"far":[26,82],"been":[27],"identified":[28],"for":[29,86],"only":[30],"2":[31],"bioluminescence":[32,61,93,111],"systems,":[33],"those":[34],"bacteria":[36],"and":[37],"fungi.":[38],"In":[39],"these":[40],"cases,":[41],"complete":[43],"reaction":[44],"cascade":[45],"genetically":[47],"encodable,":[48],"meaning":[49],"heterologous":[51],"expression":[52],"corresponding":[55],"genes":[56,103],"can":[57],"potentially":[58],"produce":[59],"autonomous":[60,92],"cell":[63],"types":[64],"other":[65],"than":[66],"bacterial":[68,110],"or":[69],"fungal":[70],"host":[71],"cells.":[72],"However,":[73],"light":[75],"levels":[76],"achieved":[77],"mammalian":[79,97],"cells":[80,98],"are":[83],"not":[84],"sufficient":[85],"single-cell":[87],"applications.":[88],"Here":[89],"we":[90],"present":[91],"images":[94],"single":[96],"coexpression":[100],"encoding":[104],"6":[106],"system.":[112]},"counts_by_year":[{"year":2026,"cited_by_count":9},{"year":2025,"cited_by_count":8},{"year":2024,"cited_by_count":8},{"year":2023,"cited_by_count":9},{"year":2022,"cited_by_count":8},{"year":2021,"cited_by_count":9},{"year":2020,"cited_by_count":19},{"year":2019,"cited_by_count":3}],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2019-12-13T00:00:00"}