{"id":"https://openalex.org/W2991677557","doi":null,"title":"[Antigen retrieval by microwave oven with buffer of citric acid].","display_name":"[Antigen retrieval by microwave oven with buffer of citric acid].","publication_year":1994,"publication_date":"1994-01-01","ids":{"openalex":"https://openalex.org/W2991677557","mag":"2991677557","pmid":"https://pubmed.ncbi.nlm.nih.gov/7997128"},"language":"es","primary_location":{"id":"pmid:7997128","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7997128","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Medicina","raw_type":"English Abstract"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"E M Pellicer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210103449","display_name":"Hospital Privado de Comunidad","ror":"https://ror.org/01jxef645","country_code":"AR","type":"healthcare","lineage":["https://openalex.org/I4210103449"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"E M Pellicer","raw_affiliation_strings":["Hospital Privado de Comunidad, Mar del Plata, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hospital Privado de Comunidad, Mar del Plata, Argentina","institution_ids":["https://openalex.org/I4210103449"]}]},{"author_position":"last","author":{"id":null,"display_name":"A Sundblad","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A Sundblad","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1999,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.61418517,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"54","issue":"2","first_page":"129","last_page":"32"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12379","display_name":"Cancer Diagnosis and Treatment","score":0.22779999673366547,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12379","display_name":"Cancer Diagnosis and Treatment","score":0.22779999673366547,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12270","display_name":"Microwave-Assisted Synthesis and Applications","score":0.14980000257492065,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.052799999713897705,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/antigen-retrieval","display_name":"antigen retrieval","score":0.875},{"id":"https://openalex.org/keywords/microwave-oven","display_name":"microwave oven","score":0.5789999961853027},{"id":"https://openalex.org/keywords/citric-acid-buffer","display_name":"citric acid buffer","score":0.9990000128746033},{"id":"https://openalex.org/keywords/tissue-fixation","display_name":"tissue fixation","score":0.8009999990463257},{"id":"https://openalex.org/keywords/immunohistochemistry","display_name":"immunohistochemistry","score":0.734000027179718},{"id":"https://openalex.org/keywords/tumor-antigens","display_name":"tumor antigens","score":0.8539999723434448},{"id":"https://openalex.org/keywords/breast-cancer","display_name":"breast cancer","score":0.8399999737739563},{"id":"https://openalex.org/keywords/prostate-cancer","display_name":"prostate cancer","score":0.9990000128746033},{"id":"https://openalex.org/keywords/melanoma","display_name":"melanoma","score":0.734000027179718},{"id":"https://openalex.org/keywords/lymphoma","display_name":"lymphoma","score":0.9520000219345093},{"id":"https://openalex.org/keywords/estrogen-receptor","display_name":"estrogen receptor","score":0.671999990940094},{"id":"https://openalex.org/keywords/progesterone-receptor","display_name":"progesterone receptor","score":0.9559999704360962},{"id":"https://openalex.org/keywords/p53","display_name":"p53","score":0.7670000195503235},{"id":"https://openalex.org/keywords/ki-67","display_name":"Ki-67","score":0.9819999933242798},{"id":"https://openalex.org/keywords/prostate-specific-antigen","display_name":"prostate-specific antigen","score":0.6899999976158142},{"id":"https://openalex.org/keywords/hmb-45","display_name":"HMB-45","score":0.6669999957084656},{"id":"https://openalex.org/keywords/vimentin","display_name":"vimentin","score":0.8270000219345093},{"id":"https://openalex.org/keywords/desmin","display_name":"desmin","score":0.8169999718666077}],"concepts":[{"id":"https://openalex.org/C2779484032","wikidata":"https://www.wikidata.org/wiki/Q25098668","display_name":"Antigen retrieval","level":3,"score":0.7778929471969604},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.7749942541122437},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6160023212432861},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.5932250022888184},{"id":"https://openalex.org/C77231918","wikidata":"https://www.wikidata.org/wiki/Q3698322","display_name":"Chromogranin A","level":3,"score":0.590103030204773},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5485308170318604},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.5110629796981812},{"id":"https://openalex.org/C2779072444","wikidata":"https://www.wikidata.org/wiki/Q425146","display_name":"Desmin","level":4,"score":0.4139217436313629},{"id":"https://openalex.org/C147447768","wikidata":"https://www.wikidata.org/wiki/Q411624","display_name":"Vimentin","level":3,"score":0.3919324278831482},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34154409170150757},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.18833580613136292}],"mesh":[{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000951","descriptor_name":"Antigens, Neoplasm","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D002021","descriptor_name":"Buffers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002951","descriptor_name":"Citrates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D008872","descriptor_name":"Microwaves","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016707","descriptor_name":"Tissue Fixation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:7997128","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7997128","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Medicina","raw_type":"English Abstract"},{"id":"mag:2991677557","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7997128","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9391000270843506,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2412080551","https://openalex.org/W2322218861","https://openalex.org/W1246919061","https://openalex.org/W147691357","https://openalex.org/W2041386436","https://openalex.org/W2100627424","https://openalex.org/W2058191082","https://openalex.org/W1981539285","https://openalex.org/W2008819273","https://openalex.org/W2305547849"],"abstract_inverted_index":{"Microwave":[0],"oven":[1],"(mwo)":[2],"is":[3],"used":[4],"to":[5,10,187],"stimulate":[6],"tissue":[7],"fixation":[8],"and":[9,21,39,72,77,106,139,146,166,170],"retrieve":[11],"antigens":[12,66,111],"damaged":[13],"by":[14],"fixation.":[15],"Heavy":[16],"metal":[17],"salt":[18],"solutions,":[19],"water,":[20],"citric":[22],"acid":[23],"buffer":[24],"(cab)":[25],"have":[26],"been":[27],"suggested":[28],"for":[29,59],"this":[30],"purpose.":[31],"A":[32],"serie":[33],"of":[34,67,87,181],"tumors":[35],"treated":[36,53],"with":[37,43,48,120,149,158,164,176],"cab":[38,83,150,177],"phosphate-buffered":[40],"saline":[41],"(pbs)":[42],"mwo":[44],"were":[45,52,63,168],"studied":[46],"immunohistochemically":[47],"24":[49],"antibodies.":[50],"Controls":[51],"in":[54,190],"the":[55,68,85,88,121,179,182],"same":[56],"way,":[57],"except":[58],"microwaving.":[60],"The":[61,79,110,153,162],"antibodies":[62,183],"directed":[64],"against":[65],"following":[69,89],"tumors:":[70],"breast":[71],"prostate":[73],"carcinoma,":[74],"carcinoid,":[75],"lymphoma":[76],"melanoma.":[78],"results":[80,163],"showed":[81],"that":[82,112,156],"enhanced":[84],"immunoreactivity":[86,180],"antigens:":[90],"estrogen":[91],"receptors":[92,95],"(AMAC),":[93],"progesterone":[94],"(Novocastra),":[96],"HMB45,":[97],"vimentin,":[98],"leukocyte":[99],"common":[100],"antigen,":[101],"PCNA,":[102],"p53,":[103],"MIB-1":[104],"(Ki-67)":[105],"prostatic":[107],"specific":[108],"antigen.":[109],"did":[113],"not":[114,171],"improve":[115],"their":[116],"immunoreactivity,":[117],"when":[118],"compared":[119],"control":[122],"series":[123],"were:":[124],"factor":[125],"VIII,":[126],"keratin,":[127],"Leu":[128],"22,":[129],"L26,":[130],"neuron-specific":[131],"enolase,":[132],"CEA,":[133],"chromogranin,":[134],"HBME-1,":[135],"smooth":[136],"muscle":[137],"actin":[138],"EMA.":[140],"Microwaving":[141],"equally":[142],"improved":[143,157],"protein":[144],"S100":[145],"desmin":[147],"either":[148],"or":[151],"pbs.":[152],"only":[154],"antigen":[155],"pbs":[159],"was":[160],"actin.":[161],"B72.3":[165],"NKI/C3":[167],"poor":[169],"reliable.":[172],"In":[173],"conclusion":[174],"microwaving":[175],"enhances":[178],"mentioned":[184],"above":[185],"leading":[186],"an":[188],"increase":[189],"sensibility":[191],"without":[192],"loosing":[193],"specificity.":[194]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2019,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2019-12-13T00:00:00"}