{"id":"https://openalex.org/W2990161599","doi":null,"title":"Analysis of xer site-specific recombination in vivo","display_name":"Analysis of xer site-specific recombination in vivo","publication_year":1992,"publication_date":"1992-01-01","ids":{"openalex":"https://openalex.org/W2990161599","mag":"2990161599"},"language":"en","primary_location":{"id":"pmh:oai:theses.gla.ac.uk:75014","doi":null,"is_oa":false,"landing_page_url":"http://theses.gla.ac.uk/75014/1/11007935.pdf","pdf_url":"http://theses.gla.ac.uk/75014/1/11007935.pdf","source":{"id":"https://openalex.org/S4306401724","display_name":"Enlighten: Theses (The University of Glasgow)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I7882870","host_organization_name":"University of Glasgow","host_organization_lineage":["https://openalex.org/I7882870"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5043965341","display_name":"Richard McCulloch","orcid":"https://orcid.org/0000-0001-5739-976X","observed_orcids":["https://orcid.org/0000-0001-5739-976X"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Richard McCulloch","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5043965341"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9926999807357788,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.004600000102072954,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/xerc","display_name":"XerC","score":0.5550000071525574},{"id":"https://openalex.org/keywords/chromosome-segregation","display_name":"chromosome segregation","score":0.7860000133514404},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.7170000076293945},{"id":"https://openalex.org/keywords/aminopeptidase-a","display_name":"aminopeptidase A","score":0.8389999866485596},{"id":"https://openalex.org/keywords/arginine-repressor","display_name":"ArgR","score":0.8140000104904175},{"id":"https://openalex.org/keywords/accessory-factors","display_name":"accessory factors","score":0.531000018119812},{"id":"https://openalex.org/keywords/homologous-recombination","display_name":"homologous recombination","score":0.7760000228881836},{"id":"https://openalex.org/keywords/plasmid-stability","display_name":"plasmid stability","score":0.4449999928474426}],"concepts":[{"id":"https://openalex.org/C156695909","wikidata":"https://www.wikidata.org/wiki/Q3373825","display_name":"Recombination","level":3,"score":0.8531906008720398},{"id":"https://openalex.org/C119898374","wikidata":"https://www.wikidata.org/wiki/Q7531737","display_name":"Site-specific recombination","level":5,"score":0.7974995374679565},{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.7871009111404419},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.6563748121261597},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.6350131034851074},{"id":"https://openalex.org/C102414288","wikidata":"https://www.wikidata.org/wiki/Q1172461","display_name":"Recombinase","level":4,"score":0.6331619620323181},{"id":"https://openalex.org/C130965112","wikidata":"https://www.wikidata.org/wiki/Q1624837","display_name":"Holliday junction","level":4,"score":0.6075078845024109},{"id":"https://openalex.org/C135300049","wikidata":"https://www.wikidata.org/wiki/Q5426559","display_name":"FLP-FRT recombination","level":5,"score":0.5788369178771973},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.483360230922699},{"id":"https://openalex.org/C101434241","wikidata":"https://www.wikidata.org/wiki/Q211675","display_name":"Genetic recombination","level":4,"score":0.4716491103172302},{"id":"https://openalex.org/C38027674","wikidata":"https://www.wikidata.org/wiki/Q4994856","display_name":"Cre-Lox recombination","level":5,"score":0.4416534900665283},{"id":"https://openalex.org/C82040048","wikidata":"https://www.wikidata.org/wiki/Q7049125","display_name":"Non-allelic homologous recombination","level":5,"score":0.4392295777797699},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.423044353723526},{"id":"https://openalex.org/C50919411","wikidata":"https://www.wikidata.org/wiki/Q5334294","display_name":"Ectopic recombination","level":5,"score":0.4162306785583496},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3737912178039551},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.0},{"id":"https://openalex.org/C141035611","wikidata":"https://www.wikidata.org/wiki/Q5532934","display_name":"Genetically modified mouse","level":4,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:theses.gla.ac.uk:75014","doi":null,"is_oa":false,"landing_page_url":"http://theses.gla.ac.uk/75014/1/11007935.pdf","pdf_url":"http://theses.gla.ac.uk/75014/1/11007935.pdf","source":{"id":"https://openalex.org/S4306401724","display_name":"Enlighten: Theses (The University of Glasgow)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I7882870","host_organization_name":"University of Glasgow","host_organization_lineage":["https://openalex.org/I7882870"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2990161599","doi":null,"is_oa":false,"landing_page_url":"https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.788495","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3010604266","https://openalex.org/W1987456338","https://openalex.org/W53165740","https://openalex.org/W2088759721","https://openalex.org/W1538904365","https://openalex.org/W2000077977","https://openalex.org/W2053286804","https://openalex.org/W141407422","https://openalex.org/W3010146489","https://openalex.org/W1997703764"],"abstract_inverted_index":{"The":[0,12,84,176,238,403,421,444,561,583],"xer":[1,46,89,117,132,164,181,304,415,439,590],"site-specific":[2,119,182,260,416,529],"recombination":[3,49,52,120,133,165,183,229,246,279,324,350,417,428,440,481,498,530,591,599],"system":[4,90,429,445],"is":[5,17,91,113,130,135,222,312,317,341,368,396,411,419,442,460],"employed":[6,412],"in":[7,18,28,60,72,92,103,180,248,287,347,390,393,426,433,454,543,566,579,600],"two":[8],"related":[9,581],"biological":[10],"processes.":[11],"first":[13],"of":[14,21,87,107,141,193,259,338,358,363,405,423,437,449,482,488,508,512,518,535,548,575,586],"these":[15,149],"roles":[16],"resolving":[19],"multimers":[20,36],"high":[22],"copy":[23],"number,":[24],"randomly":[25],"partitioned":[26,153],"plasmids":[27,75,484],"order":[29],"to":[30,43,203,244,254,283,333,399,408,502,572,588],"ensure":[31],"their":[32],"heritable":[33],"stability.":[34],"Plasmid":[35],"arise":[37],"through":[38],"homologous":[39,147],"recombination.":[40,214,402],"Their":[41],"resolution":[42],"monomers":[44,171],"by":[45,146,186,241,380,413,462,505,595,603],"site-":[47],"specific":[48,510],"requires":[50],"a":[51,114,302,447,509],"locus":[53,97],"named":[54,101,452],"cer":[55,208,226,278,321,349,401,516,528,597,606],"that":[56,112,178,314,326,373,527,539],"was":[57,299,306,352,593],"originally":[58],"described":[59,542],"the":[61,88,104,108,158,167,187,191,255,266,288,315,334,348,356,364,374,381,414,435,438,456,463,476,486,494,506,515,519,532,549,573,589,605],"naturally":[62],"occurring":[63],"plasmid":[64],"ColEl,":[65],"although":[66],"similar":[67],"sites":[68,247,517],"have":[69,503],"been":[70,99,201,217,541],"isolated":[71,565],"many":[73],"other":[74,546],"(Summers":[76],"and":[77,162,172,210,219,221,227,250,263,269,280,294,322,325,370,392,430,466,555,598,602,609],"Sherratt,":[78],"1984;":[79],"Summers":[80],"et":[81,122,126,231,235,291,558],"al,":[82,123,127,232,236,292,559],"1985).":[83],"second":[85],"role":[86,346],"bacterial":[93],"chromosome":[94,111],"partition.":[95],"A":[96,271],"has":[98,216,251,540],"described,":[100,369],"dif,":[102],"terminus":[105],"region":[106],"Escherichia":[109],"coli":[110],"substrate":[115,520,607],"for":[116,207,212,224,277,319,545],"catalysed":[118],"(Blakely":[121],"1991;":[124],"Kuempel":[125],"1991).":[128,237],"It":[129],"believed":[131,282],"activity":[134,388],"required":[136,206,223],"at":[137,355],"dif":[138,213,228,323],"because":[139],"dimers":[140,150,169],"replicating":[142],"chromosomes":[143],"are":[144,184,275,281],"produced":[145,492],"recombination;":[148],"cannot":[151],"be":[152,204,284],"into":[154,170],"daughter":[155],"cells":[156],"as":[157],"host":[159],"cell":[160,174],"divides,":[161],"hence":[163],"converts":[166],"chromosomal":[168],"allows":[173],"division.":[175],"proteins":[177,267],"act":[179],"encoded":[185,240,379],"E.coli":[188,450],"chromosome.":[189],"At":[190],"start":[192,357],"this":[194,297,359,406,587],"work":[195,298],"three":[196],"genes":[197],"{xer":[198],"genes)":[199],"had":[200],"shown":[202,501],"absolutely":[205],"recombination,":[209],"one":[211],"xerC":[215,242,459],"cloned":[218],"sequenced":[220],"both":[225,245,274,320,389],"(Colloms":[230],"1990;":[233],"Blakely":[234],"protein":[239],"binds":[243],"vitro":[249,391,544],"sequence":[252,331],"homologies":[253,332],"X":[256,335,550],"integrase":[257,336,551],"family":[258,337,552],"recombinases.":[261,339],"xerA":[262],"xerB":[264],"encode":[265],"ArgR":[268],"aminopeptidase":[270,387],"(PepA)":[272],"respectively;":[273],"essential":[276,318],"\"accessory":[285],"factors\"":[286],"reaction":[289,351,418,441,577,610],"(Stirling":[290],"1988":[293],"1989).":[295],"While":[296],"being":[300],"performed":[301],"fourth":[303],"gene":[305,365,384],"identified,":[307],"called":[308],"xprB.":[309],"Genetic":[310],"evidence":[311,371],"presented":[313,372],"XprB":[316],"it":[327],"shows":[328],"amino":[329],"acid":[330],"PepA":[340,367,410],"an":[342,424],"amino-terminal":[343],"exopeptidase":[344],"whose":[345],"not":[353],"understood":[354],"work.":[360],"Site-directed":[361],"mutagenesis":[362],"encoding":[366],"mutant":[375],"enzyme":[376],"(named":[377],"E354A)":[378],"altered":[382],"pepA":[383],"lacks":[385],"any":[386],"vivo":[394,427],"but":[395],"still":[397],"able":[398],"support":[400],"relevance":[404,585],"observation":[407],"how":[409],"discussed.":[420],"construction":[422],"controllable":[425],"its":[431,471],"use":[432],"analysing":[434],"mechanism":[436,538,592],"described.":[443],"comprises":[446],"derivative":[448],"K12,":[451],"RM40,":[453,601],"which":[455],"expression":[457],"oi":[458],"controlled":[461],"lac":[464],"promoter":[465,473],"operator":[467],"sequences":[468],"rather":[469],"than":[470],"natural":[472],"sequences.":[474],"Analysing":[475],"products":[477],"derived":[478],"from":[479],"cer-mediated":[480],"reporter":[483],"demonstrated":[485],"existence":[487],"Holliday":[489,562],"junction":[490],"structures":[491],"during":[493],"reaction.":[495],"These":[496,524],"putative":[497],"intermediates":[499,578],"were":[500,564],"arisen":[504],"exchange":[507,537],"pair":[511],"strands":[513],"within":[514],"DNA":[521],"molecule":[522],"used.":[523],"results":[525],"suggest":[526],"involves":[531],"same":[533],"form":[534],"strand":[536],"members":[547],"(Int,":[553],"FLP":[554],"Cre;":[556],"Stark":[557],"1992).":[560],"junctions":[563],"anomalously":[567],"large":[568],"quantities":[569],"when":[570],"compared":[571],"isolation":[574],"such":[576],"other,":[580],"systems.":[582],"possible":[584],"analysed":[594],"comparing":[596],"altering":[604],"molecules":[608],"conditions":[611],"employed.":[612]},"counts_by_year":[],"updated_date":"2026-10-02T10:15:12.560889","created_date":"2019-12-05T00:00:00"}