{"id":"https://openalex.org/W2989920175","doi":"https://doi.org/10.1021/acs.analchem.9b04118","title":"An Unbiased Immunoaffinity-Based Strategy for Profiling Covalent Drug Targets In Vivo","display_name":"An Unbiased Immunoaffinity-Based Strategy for Profiling Covalent Drug Targets In Vivo","publication_year":2019,"publication_date":"2019-11-19","ids":{"openalex":"https://openalex.org/W2989920175","doi":"https://doi.org/10.1021/acs.analchem.9b04118","mag":"2989920175","pmid":"https://pubmed.ncbi.nlm.nih.gov/31743002"},"language":"en","primary_location":{"id":"doi:10.1021/acs.analchem.9b04118","doi":"https://doi.org/10.1021/acs.analchem.9b04118","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.analchem.9b04118","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Xinyuan Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I2800091995","display_name":"West China Medical Center of Sichuan University","ror":"https://ror.org/040nggs60","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I24185976","https://openalex.org/I2800091995"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xinyuan Wang","raw_affiliation_strings":["Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I2800091995","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036658513","display_name":"Xiuxuan Wang","orcid":"https://orcid.org/0000-0002-4925-7048","observed_orcids":["https://orcid.org/0000-0002-4925-7048"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I2800091995","display_name":"West China Medical Center of Sichuan University","ror":"https://ror.org/040nggs60","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I24185976","https://openalex.org/I2800091995"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiuxuan Wang","raw_affiliation_strings":["Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I2800091995","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Xinghua Pu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I2800091995","display_name":"West China Medical Center of Sichuan University","ror":"https://ror.org/040nggs60","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I24185976","https://openalex.org/I2800091995"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xinghua Pu","raw_affiliation_strings":["Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I2800091995","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009878134","display_name":"Wenchen Pu","orcid":"https://orcid.org/0000-0003-0929-9566","observed_orcids":["https://orcid.org/0000-0003-0929-9566"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I2800091995","display_name":"West China Medical Center of Sichuan University","ror":"https://ror.org/040nggs60","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I24185976","https://openalex.org/I2800091995"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wenchen Pu","raw_affiliation_strings":["Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I2800091995","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101662568","display_name":"Yuqi Wang","orcid":"https://orcid.org/0000-0002-0845-6331","observed_orcids":["https://orcid.org/0000-0002-0845-6331"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I2800091995","display_name":"West China Medical Center of Sichuan University","ror":"https://ror.org/040nggs60","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I24185976","https://openalex.org/I2800091995"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yuqi Wang","raw_affiliation_strings":["Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I2800091995","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100345968","display_name":"Yu Liu","orcid":"https://orcid.org/0009-0002-2925-6563","observed_orcids":["https://orcid.org/0009-0002-2925-6563"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I2800091995","display_name":"West China Medical Center of Sichuan University","ror":"https://ror.org/040nggs60","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I24185976","https://openalex.org/I2800091995"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yu Liu","raw_affiliation_strings":["Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I2800091995","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024572713","display_name":"Yanqiu Gong","orcid":"https://orcid.org/0000-0001-7890-5158","observed_orcids":["https://orcid.org/0000-0001-7890-5158"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I2800091995","display_name":"West China Medical Center of Sichuan University","ror":"https://ror.org/040nggs60","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I24185976","https://openalex.org/I2800091995"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yanqiu Gong","raw_affiliation_strings":["Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I2800091995","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051905815","display_name":"Xiuxiu Jin","orcid":"https://orcid.org/0000-0003-2978-303X","observed_orcids":["https://orcid.org/0000-0003-2978-303X"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I2800091995","display_name":"West China Medical Center of Sichuan University","ror":"https://ror.org/040nggs60","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I24185976","https://openalex.org/I2800091995"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiuxiu Jin","raw_affiliation_strings":["Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I2800091995","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082863871","display_name":"Yong Peng","orcid":"https://orcid.org/0000-0002-1181-3467","observed_orcids":["https://orcid.org/0000-0002-1181-3467","https://orcid.org/0009-0005-1241-749X","https://orcid.org/0009-0009-9286-6306"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I2800091995","display_name":"West China Medical Center of Sichuan University","ror":"https://ror.org/040nggs60","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I24185976","https://openalex.org/I2800091995"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yong Peng","raw_affiliation_strings":["Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China"],"raw_orcid":"https://orcid.org/0000-0002-1181-3467","affiliations":[{"raw_affiliation_string":"Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I2800091995","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5080847034","display_name":"Lunzhi Dai","orcid":"https://orcid.org/0000-0002-3003-8910","observed_orcids":["https://orcid.org/0000-0002-3003-8910"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I2800091995","display_name":"West China Medical Center of Sichuan University","ror":"https://ror.org/040nggs60","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I24185976","https://openalex.org/I2800091995"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Lunzhi Dai","raw_affiliation_strings":["Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China"],"raw_orcid":"https://orcid.org/0000-0002-3003-8910","affiliations":[{"raw_affiliation_string":"Department of General Practice and National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I2800091995","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5080847034"],"corresponding_institution_ids":["https://openalex.org/I24185976","https://openalex.org/I2800091995","https://openalex.org/I4210089761","https://openalex.org/I4391768003"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":0.196,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.38024623,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":96},"biblio":{"volume":"91","issue":"24","first_page":"15818","last_page":"15825"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.5810999870300293,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.5810999870300293,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.3303000032901764,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12534","display_name":"Protein Degradation and Inhibitors","score":0.012799999676644802,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/drug-target-identification","display_name":"drug target identification","score":0.44699999690055847},{"id":"https://openalex.org/keywords/ibrutinib","display_name":"ibrutinib","score":0.8960000276565552},{"id":"https://openalex.org/keywords/bruton-tyrosine-kinase","display_name":"Bruton's tyrosine kinase","score":0.9259999990463257},{"id":"https://openalex.org/keywords/ckap4","display_name":"CKAP4","score":0.921999990940094},{"id":"https://openalex.org/keywords/tap1","display_name":"TAP1","score":0.9950000047683716},{"id":"https://openalex.org/keywords/mechanism-of-action","display_name":"mechanism of action","score":0.593999981880188}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8693385124206543},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.7765668630599976},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.6090644598007202},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5919551849365234},{"id":"https://openalex.org/C2780035454","wikidata":"https://www.wikidata.org/wiki/Q8386","display_name":"Drug","level":2,"score":0.5590669512748718},{"id":"https://openalex.org/C74187038","wikidata":"https://www.wikidata.org/wiki/Q1418791","display_name":"Drug discovery","level":2,"score":0.5498367547988892},{"id":"https://openalex.org/C171852809","wikidata":"https://www.wikidata.org/wiki/Q731154","display_name":"Chemical biology","level":2,"score":0.5326442718505859},{"id":"https://openalex.org/C2777864359","wikidata":"https://www.wikidata.org/wiki/Q5090466","display_name":"Chemical genetics","level":3,"score":0.4866219460964203},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.45548030734062195},{"id":"https://openalex.org/C90059517","wikidata":"https://www.wikidata.org/wiki/Q423572","display_name":"Bruton's tyrosine kinase","level":4,"score":0.44546568393707275},{"id":"https://openalex.org/C2779878957","wikidata":"https://www.wikidata.org/wiki/Q5984881","display_name":"Ibrutinib","level":4,"score":0.4417515695095062},{"id":"https://openalex.org/C161624437","wikidata":"https://www.wikidata.org/wiki/Q1988322","display_name":"Small molecule","level":2,"score":0.35234740376472473},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.34863537549972534},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.3289349675178528},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.17347615957260132},{"id":"https://openalex.org/C42362537","wikidata":"https://www.wikidata.org/wiki/Q87099740","display_name":"Tyrosine kinase","level":3,"score":0.0},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0},{"id":"https://openalex.org/C2777938653","wikidata":"https://www.wikidata.org/wiki/Q1088156","display_name":"Chronic lymphocytic leukemia","level":3,"score":0.0},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.0},{"id":"https://openalex.org/C2778461978","wikidata":"https://www.wikidata.org/wiki/Q29496","display_name":"Leukemia","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000071181","descriptor_name":"ATP Binding Cassette Transporter, Subfamily B, Member 2","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000071181","descriptor_name":"ATP Binding Cassette Transporter, Subfamily B, Member 2","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D000077329","descriptor_name":"Agammaglobulinaemia Tyrosine Kinase","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D000077329","descriptor_name":"Agammaglobulinaemia Tyrosine Kinase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000225","descriptor_name":"Adenine","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000906","descriptor_name":"Antibodies","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D008810","descriptor_name":"Mice, Inbred C57BL","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D010880","descriptor_name":"Piperidines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011720","descriptor_name":"Pyrazoles","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D011720","descriptor_name":"Pyrazoles","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011720","descriptor_name":"Pyrazoles","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011743","descriptor_name":"Pyrimidines","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D011743","descriptor_name":"Pyrimidines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011743","descriptor_name":"Pyrimidines","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D013154","descriptor_name":"Spleen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013154","descriptor_name":"Spleen","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D013154","descriptor_name":"Spleen","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1021/acs.analchem.9b04118","doi":"https://doi.org/10.1021/acs.analchem.9b04118","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.analchem.9b04118","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:31743002","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31743002","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1101615596","display_name":null,"funder_award_id":"2018YFC200004","funder_id":"https://openalex.org/F4320321540","funder_display_name":"Ministry of Science and Technology of the People's Republic of China"},{"id":"https://openalex.org/G2356424354","display_name":null,"funder_award_id":"31870826","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G5516573790","display_name":"\u86cb\u767d\u8d28\u65b0\u4fee\u9970\u8d56\u6c28\u9178-2-\u7f9f\u57fa\u5f02\u4e01\u9170\u5316\u53ca\u5176\u8c03\u63a7\u9176\u5728\u80ba\u7ea4\u7ef4\u5316\u8fc7\u7a0b\u4e2d\u7684\u4f5c\u7528\u4e0e\u673a\u5236","funder_award_id":"81570060","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G6774520716","display_name":null,"funder_award_id":"2018YFC1312301","funder_id":"https://openalex.org/F4320321540","funder_display_name":"Ministry of Science and Technology of the People's Republic of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320321540","display_name":"Ministry of Science and Technology of the People's Republic of China","ror":"https://ror.org/027s68j25"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W1526377515","https://openalex.org/W1830209188","https://openalex.org/W1975013268","https://openalex.org/W2010993054","https://openalex.org/W2021790986","https://openalex.org/W2025703773","https://openalex.org/W2038252445","https://openalex.org/W2070719504","https://openalex.org/W2077605453","https://openalex.org/W2093859044","https://openalex.org/W2114163388","https://openalex.org/W2134358746","https://openalex.org/W2294450123","https://openalex.org/W2441236057","https://openalex.org/W2517550655","https://openalex.org/W2705421977","https://openalex.org/W2752520345","https://openalex.org/W2757007104","https://openalex.org/W2765241646","https://openalex.org/W2902716259","https://openalex.org/W2933867250","https://openalex.org/W2972163730"],"related_works":["https://openalex.org/W4200556719","https://openalex.org/W2211426991","https://openalex.org/W3212077312","https://openalex.org/W2751438857","https://openalex.org/W2335094803","https://openalex.org/W2793119780","https://openalex.org/W4400258795","https://openalex.org/W2992345293","https://openalex.org/W2019563714","https://openalex.org/W4307092421"],"abstract_inverted_index":{"Activity-based":[0],"chemical":[1,24,121,130],"proteomics":[2],"approaches":[3],"used":[4],"for":[5,53],"identifying":[6,54],"cellular":[7],"targets":[8,57],"of":[9,17,40,123,129,140,142],"drugs":[10,31,41],"are":[11,26,108],"mainly":[12],"dependent":[13],"on":[14],"the":[15,30,37,62,69,120,126,137],"availability":[16],"probes":[18,25],"derived":[19],"from":[20,29],"drugs.":[21,144],"However,":[22],"all":[23],"structurally":[27],"different":[28],"themselves":[32],"and":[33,49,90,132],"cannot":[34],"fully":[35],"mimic":[36],"real":[38],"actions":[39],"in":[42,58,86,92,111,115],"cells.":[43],"Here":[44],"we":[45,65],"present":[46],"a":[47],"concise":[48],"unbiased":[50],"immunoaffinity-based":[51],"strategy":[52],"covalent":[55,143],"drug":[56,106],"vivo.":[59],"By":[60],"using":[61],"specific":[63],"antibody,":[64],"not":[66,114],"only":[67],"confirm":[68],"well-known":[70],"ibrutinib-binding":[71],"target":[72,97],"BTK,":[73],"but":[74,113],"also":[75],"identify":[76],"some":[77],"previously":[78],"undescribed":[79],"strongly":[80],"binding":[81],"proteins,":[82],"such":[83],"as":[84],"CKAP4":[85],"human":[87],"cell":[88],"lines":[89],"TAP1":[91],"mouse":[93],"organs.":[94],"The":[95],"observed":[96],"profiles":[98],"between":[99],"species":[100],"may":[101],"partially":[102],"explain":[103],"why":[104],"certain":[105],"candidates":[107],"very":[109],"effective":[110],"mice":[112],"humans.":[116],"This":[117],"approach":[118],"avoids":[119],"modification":[122],"drugs,":[124],"eliminates":[125],"nonspecific":[127],"bindings":[128],"probes,":[131],"allows":[133],"to":[134],"unbiasedly":[135],"decode":[136],"underlying":[138],"mechanisms":[139],"action":[141]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2022,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2019-12-05T00:00:00"}