{"id":"https://openalex.org/W2989809047","doi":"https://doi.org/10.1021/jacs.9b07111","title":"Nonribosomal Peptide Extension by a Peptide Amino-Acyl tRNA Ligase","display_name":"Nonribosomal Peptide Extension by a Peptide Amino-Acyl tRNA Ligase","publication_year":2019,"publication_date":"2019-11-21","ids":{"openalex":"https://openalex.org/W2989809047","doi":"https://doi.org/10.1021/jacs.9b07111","mag":"2989809047","pmid":"https://pubmed.ncbi.nlm.nih.gov/31751505","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6927032"},"language":"en","primary_location":{"id":"doi:10.1021/jacs.9b07111","doi":"https://doi.org/10.1021/jacs.9b07111","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jacs.9b07111","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6927032","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5034359034","display_name":"Zhengan Zhang","orcid":"https://orcid.org/0000-0003-3993-1163","observed_orcids":["https://orcid.org/0000-0003-3993-1163"]},"institutions":[{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zhengan Zhang","raw_affiliation_strings":["Department of Chemistry, University of Illinois at Urbana\u2212Champaign, Urbana, Illinois 61801, United States"],"raw_orcid":"https://orcid.org/0000-0003-3993-1163","affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Illinois at Urbana\u2212Champaign, Urbana, Illinois 61801, United States","institution_ids":["https://openalex.org/I157725225"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067523147","display_name":"Wilfred A. van der Donk","orcid":"https://orcid.org/0000-0002-5467-7071","observed_orcids":["https://orcid.org/0000-0002-5467-7071"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I157725225","display_name":"University of Illinois Urbana-Champaign","ror":"https://ror.org/047426m28","country_code":"US","type":"education","lineage":["https://openalex.org/I157725225"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Wilfred A. van der Donk","raw_affiliation_strings":["Department of Chemistry, University of Illinois at Urbana\u2212Champaign, Urbana, Illinois 61801, United States","Howard Hughes Medical Institute, University of Illinois at Urbana\u2212Champaign, Urbana, Illinois 61801, United States"],"raw_orcid":"https://orcid.org/0000-0002-5467-7071","affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Illinois at Urbana\u2212Champaign, Urbana, Illinois 61801, United States","institution_ids":["https://openalex.org/I157725225"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, University of Illinois at Urbana\u2212Champaign, Urbana, Illinois 61801, United States","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I157725225"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5067523147"],"corresponding_institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I157725225"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":1.9681,"has_fulltext":false,"cited_by_count":23,"citation_normalized_percentile":{"value":0.84358323,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"141","issue":"50","first_page":"19625","last_page":"19633"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.7621999979019165,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.7621999979019165,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.21529999375343323,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.005400000140070915,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pearl","display_name":"pearls","score":0.9330000281333923},{"id":"https://openalex.org/keywords/nonribosomal-peptide-biosynthesis","display_name":"nonribosomal peptide biosynthesis","score":0.8429999947547913},{"id":"https://openalex.org/keywords/pseudomonas-syringae","display_name":"Pseudomonas syringae","score":0.9729999899864197},{"id":"https://openalex.org/keywords/atp-dependent-phosphorylation","display_name":"ATP-dependent phosphorylation","score":0.5580000281333923},{"id":"https://openalex.org/keywords/ping-pong-mechanism","display_name":"ping-pong mechanism","score":0.8420000076293945},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.7210000157356262},{"id":"https://openalex.org/keywords/isotopic-labeling","display_name":"isotopic labeling","score":0.781000018119812}],"concepts":[{"id":"https://openalex.org/C2777379556","wikidata":"https://www.wikidata.org/wiki/Q1443801","display_name":"Nonribosomal peptide","level":4,"score":0.7946140170097351},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7497748732566833},{"id":"https://openalex.org/C175114707","wikidata":"https://www.wikidata.org/wiki/Q408483","display_name":"DNA ligase","level":3,"score":0.7075002193450928},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.6575624942779541},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.6407377123832703},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5799819827079773},{"id":"https://openalex.org/C162776295","wikidata":"https://www.wikidata.org/wiki/Q901154","display_name":"Semisynthesis","level":2,"score":0.550459623336792},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.46175312995910645},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.43328022956848145},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.368577241897583},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3612109422683716},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010455","descriptor_name":"Peptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011130","descriptor_name":"RNA Ligase (ATP)","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011130","descriptor_name":"RNA Ligase (ATP)","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012346","descriptor_name":"RNA, Transfer, Amino Acyl","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1021/jacs.9b07111","doi":"https://doi.org/10.1021/jacs.9b07111","is_oa":false,"landing_page_url":"https://doi.org/10.1021/jacs.9b07111","pdf_url":null,"source":{"id":"https://openalex.org/S111155417","display_name":"Journal of the American Chemical Society","issn_l":"0002-7863","issn":["0002-7863","1520-5126","1943-2984"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"journal-article"},{"id":"pmid:31751505","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31751505","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Chemical Society","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6927032","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6927032","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Am Chem Soc","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:6927032","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6927032","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Am Chem Soc","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5667087312","display_name":null,"funder_award_id":"GM R01 058822","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G6968381938","display_name":"Posttranslational Modifications in Natural Product Biosynthesis","funder_award_id":"R01GM058822","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G9000889708","display_name":"Posttranslational Modifications during Natural Product Biosynthesis","funder_award_id":"R37GM058822","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W1541441427","https://openalex.org/W1587997563","https://openalex.org/W1697971154","https://openalex.org/W1803102843","https://openalex.org/W1968917608","https://openalex.org/W1972011132","https://openalex.org/W1975920595","https://openalex.org/W2017980138","https://openalex.org/W2019729932","https://openalex.org/W2021522936","https://openalex.org/W2023397345","https://openalex.org/W2028820703","https://openalex.org/W2029264006","https://openalex.org/W2040096973","https://openalex.org/W2062932139","https://openalex.org/W2063869948","https://openalex.org/W2064080420","https://openalex.org/W2066519322","https://openalex.org/W2070424221","https://openalex.org/W2078216263","https://openalex.org/W2080471041","https://openalex.org/W2115457325","https://openalex.org/W2162854463","https://openalex.org/W2186689554","https://openalex.org/W2231071237","https://openalex.org/W2252545027","https://openalex.org/W2280897757","https://openalex.org/W2514135216","https://openalex.org/W2569920290","https://openalex.org/W2960430351","https://openalex.org/W2967524995"],"related_works":["https://openalex.org/W4414990409","https://openalex.org/W4408611837","https://openalex.org/W4283830725","https://openalex.org/W3210971486","https://openalex.org/W3083526414","https://openalex.org/W4224001680","https://openalex.org/W4225399837","https://openalex.org/W7220756719","https://openalex.org/W2805164408","https://openalex.org/W99885311"],"abstract_inverted_index":{"The":[0,49,129],"catalytic":[1],"use":[2],"of":[3,11,38,51,70,79,84,91,109,125,135,144,157,174,203,222],"a":[4,17,26,39,64,73,209],"small":[5,40],"peptide":[6,41,86],"scaffold":[7,42],"for":[8,166,212],"the":[9,36,77,82,85,89,94,107,121,132,150,172,182,200,206,213],"biosynthesis":[10,90],"amino":[12,33,123,183,214],"acid-derived":[13],"natural":[14],"products":[15],"is":[16,55,116,195],"recently":[18],"discovered":[19],"new":[20],"biosynthetic":[21],"strategy.":[22],"During":[23],"this":[24,52,60,220],"process,":[25],"peptide-amino":[27],"acyl":[28],"tRNA":[29,136,146],"ligase":[30],"(PEARL)":[31],"adds":[32],"acids":[34],"to":[35,81,106,111,120,180,197],"C-terminus":[37,83,108],"in":[43,88,93,160],"an":[44],"ATP-":[45],"and":[46,67,115,140,168],"tRNA-dependent":[47],"process.":[48],"mechanism":[50,211],"unusual":[53],"transformation":[54],"currently":[56],"not":[57,170],"known.":[58],"In":[59],"study,":[61],"we":[62,191],"present":[63],"detailed":[65],"biochemical":[66],"mechanistic":[68],"study":[69],"TglB":[71,100,161,175],"(UniProtKB-F3HQJ1),":[72],"PEARL":[74],"that":[75,148,193],"catalyzes":[76],"addition":[78,185,216],"Cys":[80,147],"TglA":[87,110],"3-thiaglutamate":[92],"plant":[95],"pathogen":[96],"Pseudomonas":[97],"syringae":[98],".":[99],"recognizes":[101,131],"several":[102,163],"important":[103],"residues":[104,159,165],"close":[105],"perform":[112],"its":[113,126],"activity":[114],"tolerant":[117],"with":[118],"respect":[119],"last":[122],"acid":[124,184,215],"substrate":[127],"peptide.":[128],"enzyme":[130],"acceptor":[133,151],"stem":[134],"Cys,":[137],"as":[138],"micro-":[139],"minihelices,":[141],"truncated":[142],"versions":[143],"full-length":[145],"contain":[149],"stem,":[152],"were":[153],"also":[154],"accepted.":[155],"Mutagenesis":[156],"conserved":[158],"identified":[162],"key":[164],"catalysis":[167],"did":[169],"support":[171,208],"possibility":[173],"adopting":[176],"various":[177],"ping-pong":[178],"mechanisms":[179],"catalyze":[181],"reaction.":[186],"Using":[187],"isotopic":[188],"labeling":[189],"studies,":[190],"demonstrate":[192],"ATP":[194],"used":[196],"directly":[198],"phosphorylate":[199],"C-terminal":[201],"carboxylate":[202],"TglA.":[204],"Collectively,":[205],"data":[207],"general":[210],"reaction":[217],"catalyzed":[218],"by":[219],"class":[221],"enzyme.":[223]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":6}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2019-12-05T00:00:00"}