{"id":"https://openalex.org/W2980692987","doi":"https://doi.org/10.3791/60310","title":"Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants","display_name":"Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants","publication_year":2019,"publication_date":"2019-10-15","ids":{"openalex":"https://openalex.org/W2980692987","doi":"https://doi.org/10.3791/60310","mag":"2980692987","pmid":"https://pubmed.ncbi.nlm.nih.gov/31680669","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7577426"},"language":"en","primary_location":{"id":"doi:10.3791/60310","doi":"https://doi.org/10.3791/60310","is_oa":false,"landing_page_url":"https://doi.org/10.3791/60310","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7577426","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060983869","display_name":"Megan E. Vaughan","orcid":"https://orcid.org/0000-0002-8289-0638","observed_orcids":["https://orcid.org/0000-0002-8289-0638"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Megan Vaughan","raw_affiliation_strings":["Scripps Research, Department of Molecular Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Scripps Research, Department of Molecular Medicine","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073949817","display_name":"Katja A Lamia","orcid":"https://orcid.org/0000-0001-9533-0499","observed_orcids":["https://orcid.org/0000-0001-9533-0499"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Katja A. Lamia","raw_affiliation_strings":["Scripps Research, Department of Molecular Medicine; klamia@scripps.edu","Scripps Research, Department of Molecular Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Scripps Research, Department of Molecular Medicine; klamia@scripps.edu","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"Scripps Research, Department of Molecular Medicine","institution_ids":["https://openalex.org/I123431417"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I123431417"],"apc_list":null,"apc_paid":null,"fwci":0.2459,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.48168906,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":null,"issue":"152","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11457","display_name":"Adipose Tissue and Metabolism","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10333","display_name":"Meat and Animal Product Quality","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/primary-myoblasts","display_name":"primary myoblasts","score":0.9789999723434448},{"id":"https://openalex.org/keywords/myogenesis","display_name":"myogenesis","score":0.9350000023841858},{"id":"https://openalex.org/keywords/muscle-precursors","display_name":"muscle precursors","score":0.6570000052452087},{"id":"https://openalex.org/keywords/mouse-skeletal-muscle","display_name":"mouse skeletal muscle","score":0.6650000214576721}],"concepts":[{"id":"https://openalex.org/C207200792","wikidata":"https://www.wikidata.org/wiki/Q428914","display_name":"Myocyte","level":2,"score":0.79269939661026},{"id":"https://openalex.org/C2779959927","wikidata":"https://www.wikidata.org/wiki/Q1048687","display_name":"Skeletal muscle","level":2,"score":0.7864034175872803},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6637455224990845},{"id":"https://openalex.org/C60278653","wikidata":"https://www.wikidata.org/wiki/Q5008075","display_name":"C2C12","level":4,"score":0.6379297971725464},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6180685758590698},{"id":"https://openalex.org/C148738053","wikidata":"https://www.wikidata.org/wiki/Q210861","display_name":"Cellular differentiation","level":3,"score":0.5077375173568726},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.4726383090019226},{"id":"https://openalex.org/C3763915","wikidata":"https://www.wikidata.org/wiki/Q3331437","display_name":"Myogenesis","level":3,"score":0.45499783754348755},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4504096806049347},{"id":"https://openalex.org/C157890192","wikidata":"https://www.wikidata.org/wiki/Q2561978","display_name":"Explant culture","level":3,"score":0.44082844257354736},{"id":"https://openalex.org/C26291073","wikidata":"https://www.wikidata.org/wiki/Q310484","display_name":"Ex vivo","level":3,"score":0.4329046607017517},{"id":"https://openalex.org/C147905033","wikidata":"https://www.wikidata.org/wiki/Q1378887","display_name":"Primary cell","level":3,"score":0.4201132655143738},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.41148656606674194},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.2705604135990143},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.16145989298820496},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14397162199020386},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09743142127990723},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.08764871954917908},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.05308115482330322},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009924","descriptor_name":"Organ Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018482","descriptor_name":"Muscle, Skeletal","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D018482","descriptor_name":"Muscle, Skeletal","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D024510","descriptor_name":"Muscle Development","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D032446","descriptor_name":"Myoblasts","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.3791/60310","doi":"https://doi.org/10.3791/60310","is_oa":false,"landing_page_url":"https://doi.org/10.3791/60310","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"doi:10.3791/60310-v","doi":"https://doi.org/10.3791/60310-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/60310-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmid:31680669","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31680669","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7577426","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7577426","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Vis Exp","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:7577426","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7577426","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Vis Exp","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.4043000042438507}],"study_designs":[],"awards":[{"id":"https://openalex.org/G184299350","display_name":"Circadian molecular regulation of the xenobiotic response","funder_award_id":"R01DK097164","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G2932620295","display_name":"Regulation of exercise physiology by mammalian cryptochromes","funder_award_id":"R01DK112927","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W8245821","https://openalex.org/W1639947976","https://openalex.org/W1797874859","https://openalex.org/W1969747015","https://openalex.org/W1976972286","https://openalex.org/W2020575559","https://openalex.org/W2028026223","https://openalex.org/W2056972142","https://openalex.org/W2062867597","https://openalex.org/W2083405476","https://openalex.org/W2115357041","https://openalex.org/W2116924365","https://openalex.org/W2144170321","https://openalex.org/W2590973500","https://openalex.org/W2596171931","https://openalex.org/W2610198299","https://openalex.org/W2741361096","https://openalex.org/W2797215615"],"related_works":["https://openalex.org/W2613605621","https://openalex.org/W3042872538","https://openalex.org/W4408315205","https://openalex.org/W2803741393","https://openalex.org/W7131211788","https://openalex.org/W4414751315","https://openalex.org/W2283717565","https://openalex.org/W2409005788","https://openalex.org/W2348835256","https://openalex.org/W2039321761"],"abstract_inverted_index":{"Primary":[0],"myoblasts":[1],"are":[2,58],"undifferentiated":[3],"proliferating":[4],"precursors":[5,17],"of":[6,25,41,46,63,67,70,95,100,107,111,128,157],"skeletal":[7,53,68],"muscle.":[8],"They":[9],"can":[10,149],"be":[11,150],"cultured":[12],"and":[13,39,97,109,152],"studied":[14,153],"as":[15,74,76,82],"muscle":[16,26,54,69,130],"or":[18,87],"induced":[19],"to":[20,115],"differentiate":[21],"into":[22],"later":[23],"stages":[24],"development.":[27],"The":[28,93],"protocol":[29],"provided":[30],"here":[31,143],"describes":[32],"a":[33,42,122,145],"robust":[34,123],"method":[35],"for":[36,60,125],"the":[37,61,64,105,112,126,140],"isolation":[38],"culture":[40],"highly":[43],"proliferative":[44],"population":[45,147],"myoblast":[47,117],"cells":[48,57,102],"from":[49],"young":[50],"adult":[51],"mouse":[52,72,129],"explants.":[55],"These":[56,119],"useful":[59],"study":[62,127],"metabolic":[65,98],"properties":[66],"different":[71],"models,":[73,139],"well":[75],"in":[77,137],"other":[78],"downstream":[79],"applications":[80],"such":[81],"transfection":[83],"with":[84,89,154],"exogenous":[85],"DNA":[86],"transduction":[88],"viral":[90],"expression":[91],"vectors.":[92],"level":[94],"differentiation":[96],"profile":[99],"these":[101],"depends":[103],"on":[104],"length":[106],"exposure,":[108],"composition":[110],"media":[113],"used":[114],"induce":[116],"differentiation.":[118],"methods":[120,141],"provide":[121,144],"system":[124],"cell":[131,146],"metabolism":[132],"ex":[133],"vivo.":[134],"Importantly,":[135],"unlike":[136],"vivo":[138],"described":[142],"that":[148],"expanded":[151],"high":[155],"levels":[156],"reproducibility.":[158]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":2},{"year":2020,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2019-10-25T00:00:00"}
