{"id":"https://openalex.org/W2980076216","doi":"https://doi.org/10.1093/protein/gzz032","title":"Toward the design of efficient transglycosidases: the case of the GH1 of Thermus thermophilus","display_name":"Toward the design of efficient transglycosidases: the case of the GH1 of Thermus thermophilus","publication_year":2019,"publication_date":"2019-07-24","ids":{"openalex":"https://openalex.org/W2980076216","doi":"https://doi.org/10.1093/protein/gzz032","mag":"2980076216","pmid":"https://pubmed.ncbi.nlm.nih.gov/31603224"},"language":"en","primary_location":{"id":"doi:10.1093/protein/gzz032","doi":"https://doi.org/10.1093/protein/gzz032","is_oa":false,"landing_page_url":"https://doi.org/10.1093/protein/gzz032","pdf_url":null,"source":{"id":"https://openalex.org/S44492648","display_name":"Protein Engineering Design and Selection","issn_l":"0269-2139","issn":["0269-2139","1460-213X","1741-0126","1741-0134"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Engineering, Design and Selection","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040957504","display_name":"Beno\u00eet David","orcid":"https://orcid.org/0000-0003-1622-9312","observed_orcids":["https://orcid.org/0000-0003-1622-9312"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4387154840","display_name":"Unit\u00e9 en Sciences Biologiques et Biotechnologies de Nantes","ror":"https://ror.org/05ef4v550","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4387154840","https://openalex.org/I97188460"]},{"id":"https://openalex.org/I44260953","display_name":"Heinrich Heine University D\u00fcsseldorf","ror":"https://ror.org/024z2rq82","country_code":"DE","type":"education","lineage":["https://openalex.org/I44260953"]},{"id":"https://openalex.org/I97188460","display_name":"Nantes Universit\u00e9","ror":"https://ror.org/03gnr7b55","country_code":"FR","type":"education","lineage":["https://openalex.org/I97188460"]}],"countries":["DE","FR"],"is_corresponding":false,"raw_author_name":"Benoit David","raw_affiliation_strings":["CPC, Heinrich-Heine-Universit\u00e4t, 40225 D\u00fcsseldorf, Germany","UFIP, UMR 6286 of CNRS, University of Nantes, 44035 Nantes, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CPC, Heinrich-Heine-Universit\u00e4t, 40225 D\u00fcsseldorf, Germany","institution_ids":["https://openalex.org/I44260953"]},{"raw_affiliation_string":"UFIP, UMR 6286 of CNRS, University of Nantes, 44035 Nantes, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4387154840","https://openalex.org/I97188460"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Philippe Arnaud","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4387154840","display_name":"Unit\u00e9 en Sciences Biologiques et Biotechnologies de Nantes","ror":"https://ror.org/05ef4v550","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4387154840","https://openalex.org/I97188460"]},{"id":"https://openalex.org/I97188460","display_name":"Nantes Universit\u00e9","ror":"https://ror.org/03gnr7b55","country_code":"FR","type":"education","lineage":["https://openalex.org/I97188460"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Philippe Arnaud","raw_affiliation_strings":["UFIP, UMR 6286 of CNRS, University of Nantes, 44035 Nantes, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UFIP, UMR 6286 of CNRS, University of Nantes, 44035 Nantes, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4387154840","https://openalex.org/I97188460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103248784","display_name":"Charles Tellier","orcid":"https://orcid.org/0000-0002-5788-2847","observed_orcids":["https://orcid.org/0000-0002-5788-2847"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4387154840","display_name":"Unit\u00e9 en Sciences Biologiques et Biotechnologies de Nantes","ror":"https://ror.org/05ef4v550","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4387154840","https://openalex.org/I97188460"]},{"id":"https://openalex.org/I97188460","display_name":"Nantes Universit\u00e9","ror":"https://ror.org/03gnr7b55","country_code":"FR","type":"education","lineage":["https://openalex.org/I97188460"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Charles Tellier","raw_affiliation_strings":["UFIP, UMR 6286 of CNRS, University of Nantes, 44035 Nantes, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"UFIP, UMR 6286 of CNRS, University of Nantes, 44035 Nantes, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4387154840","https://openalex.org/I97188460"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5060452581","display_name":"Yves\u2010Henri Sanejouand","orcid":"https://orcid.org/0000-0001-8589-2666","observed_orcids":["https://orcid.org/0000-0001-8589-2666"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4387154840","display_name":"Unit\u00e9 en Sciences Biologiques et Biotechnologies de Nantes","ror":"https://ror.org/05ef4v550","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I4387154840","https://openalex.org/I97188460"]},{"id":"https://openalex.org/I97188460","display_name":"Nantes Universit\u00e9","ror":"https://ror.org/03gnr7b55","country_code":"FR","type":"education","lineage":["https://openalex.org/I97188460"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Yves-Henri Sanejouand","raw_affiliation_strings":["UFIP, UMR 6286 of CNRS, University of Nantes, 44035 Nantes, France"],"raw_orcid":"https://orcid.org/0000-0001-8589-2666","affiliations":[{"raw_affiliation_string":"UFIP, UMR 6286 of CNRS, University of Nantes, 44035 Nantes, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4387154840","https://openalex.org/I97188460"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5060452581"],"corresponding_institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4387154840","https://openalex.org/I97188460"],"apc_list":{"value":4656,"currency":"USD","value_usd":4656},"apc_paid":null,"fwci":0.43,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.56792469,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"32","issue":"7","first_page":"309","last_page":"316"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.760699987411499,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.760699987411499,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.15690000355243683,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.05040000006556511,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glycoside-hydrolase-family-1","display_name":"glycoside hydrolase family 1","score":0.9509999752044678},{"id":"https://openalex.org/keywords/thermus-thermophilus","display_name":"Thermus thermophilus","score":0.9919999837875366},{"id":"https://openalex.org/keywords/enzyme-engineering","display_name":"enzyme engineering","score":0.7179999947547913},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.8690000176429749},{"id":"https://openalex.org/keywords/catalytic-efficiency","display_name":"catalytic efficiency","score":0.8659999966621399},{"id":"https://openalex.org/keywords/molecular-dynamics-simulation","display_name":"molecular dynamics simulation","score":0.546999990940094}],"concepts":[{"id":"https://openalex.org/C2780883830","wikidata":"https://www.wikidata.org/wiki/Q139470","display_name":"Thermus thermophilus","level":4,"score":0.9949410557746887},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6812425851821899},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.6545038223266602},{"id":"https://openalex.org/C196032511","wikidata":"https://www.wikidata.org/wiki/Q375795","display_name":"Glycoside hydrolase","level":3,"score":0.6487741470336914},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5662157535552979},{"id":"https://openalex.org/C2780598303","wikidata":"https://www.wikidata.org/wiki/Q65921492","display_name":"Flexibility (engineering)","level":2,"score":0.48062270879745483},{"id":"https://openalex.org/C2780120296","wikidata":"https://www.wikidata.org/wiki/Q96286","display_name":"Hydrolase","level":3,"score":0.46621567010879517},{"id":"https://openalex.org/C207583985","wikidata":"https://www.wikidata.org/wiki/Q128723","display_name":"Wild type","level":4,"score":0.45870694518089294},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3953571319580078},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3700384199619293},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.25267714262008667},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.1378045678138733},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.09438428282737732},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.09364920854568481},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C105795698","wikidata":"https://www.wikidata.org/wiki/Q12483","display_name":"Statistics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006031","descriptor_name":"Glycosylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016963","descriptor_name":"Thermus thermophilus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D016963","descriptor_name":"Thermus thermophilus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D016963","descriptor_name":"Thermus thermophilus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056004","descriptor_name":"Molecular Dynamics Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056004","descriptor_name":"Molecular Dynamics Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056004","descriptor_name":"Molecular Dynamics Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1093/protein/gzz032","doi":"https://doi.org/10.1093/protein/gzz032","is_oa":false,"landing_page_url":"https://doi.org/10.1093/protein/gzz032","pdf_url":null,"source":{"id":"https://openalex.org/S44492648","display_name":"Protein Engineering Design and Selection","issn_l":"0269-2139","issn":["0269-2139","1460-213X","1741-0126","1741-0134"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Engineering, Design and Selection","raw_type":"journal-article"},{"id":"pmid:31603224","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31603224","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein engineering, design & selection : PEDS","raw_type":"Journal Article"},{"id":"pmh:oai:HAL:hal-02322263v1","doi":null,"is_oa":false,"landing_page_url":"https://hal.science/hal-02322263","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL (Le Centre pour la Communication Scientifique Directe)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1294671590","host_organization_name":"Centre National de la Recherche Scientifique","host_organization_lineage":["https://openalex.org/I1294671590"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Protein Engineering, Design and Selection, 2019, 32 (7), pp.309-316. ⟨10.1093/protein/gzz032⟩","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Clean water and sanitation","score":0.46000000834465027,"id":"https://metadata.un.org/sdg/6"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320326256","display_name":"Grand \u00c9quipement National De Calcul Intensif","ror":"https://ror.org/0010d1q40"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":43,"referenced_works":["https://openalex.org/W225107651","https://openalex.org/W1580799101","https://openalex.org/W1967680131","https://openalex.org/W1976499671","https://openalex.org/W1976825191","https://openalex.org/W1987250042","https://openalex.org/W1987281470","https://openalex.org/W1989202961","https://openalex.org/W1995878037","https://openalex.org/W1999233126","https://openalex.org/W2001296212","https://openalex.org/W2002105808","https://openalex.org/W2014035737","https://openalex.org/W2035266068","https://openalex.org/W2054964992","https://openalex.org/W2067174909","https://openalex.org/W2076301285","https://openalex.org/W2081693079","https://openalex.org/W2098943363","https://openalex.org/W2102537419","https://openalex.org/W2107029002","https://openalex.org/W2128406883","https://openalex.org/W2128572087","https://openalex.org/W2132262459","https://openalex.org/W2132629607","https://openalex.org/W2138619548","https://openalex.org/W2156125289","https://openalex.org/W2161605421","https://openalex.org/W2165185956","https://openalex.org/W2171268876","https://openalex.org/W2187348079","https://openalex.org/W2322040949","https://openalex.org/W2323199602","https://openalex.org/W2323927982","https://openalex.org/W2376089928","https://openalex.org/W2411846742","https://openalex.org/W2417466171","https://openalex.org/W2605067699","https://openalex.org/W2729699231","https://openalex.org/W2737905053","https://openalex.org/W2751544302","https://openalex.org/W2911390449","https://openalex.org/W4234253662"],"related_works":["https://openalex.org/W4214901071","https://openalex.org/W1963721914","https://openalex.org/W2977815930","https://openalex.org/W2047846812","https://openalex.org/W4283118979","https://openalex.org/W1995805256","https://openalex.org/W2744475623","https://openalex.org/W1977450486","https://openalex.org/W2318644970","https://openalex.org/W2091034002"],"abstract_inverted_index":{"Using":[0],"the":[1,5,17,21,26,51,72,78,81,84,108,111,122,125],"information":[2],"available":[3],"in":[4,11,16,77,107],"sequences":[6],"of":[7,20,28,57,83,94,110,124,127],"well-characterized":[8],"transglycosidases":[9],"found":[10,88],"plants,":[12],"mutations":[13],"were":[14],"introduced":[15],"glycoside":[18,130],"hydrolase":[19,131],"bacterium":[22],"Thermus":[23],"thermophilus,":[24],"with":[25],"aim":[27],"turning":[29],"it":[30,139],"into":[31,140],"an":[32,141],"efficient":[33,49,70,142],"transglycosidase.":[34,143],"All":[35],"mutants":[36],"happen":[37],"to":[38,90,137],"have":[39],"fair":[40],"catalytic":[41],"efficiencies,":[42],"being":[43],"at":[44],"worst":[45],"25":[46],"times":[47,68],"less":[48,69],"than":[50,71],"wild":[52,73,79],"type.":[53,74],"Noteworthy,":[54],"W120F,":[55],"one":[56],"our":[58],"high":[59,112],"transglycosylation":[60,113],"yield":[61,114],"(\u2248":[62],"50%)":[63],"mutants,":[64],"is":[65,86,104],"only":[66,87],"two":[67],"Interestingly,":[75],"while":[76],"type":[80],"sidechain":[82],"acid-base":[85,126],"able":[89],"sample":[91],"a":[92,128,134],"pair":[93],"equivalent":[95],"conformations":[96],"during":[97],"0.5-\u03bcs-long":[98],"molecular":[99],"dynamics":[100],"simulations,":[101],"its":[102],"flexibility":[103,123],"much":[105],"higher":[106],"case":[109],"mutants.":[115],"Our":[116],"results":[117],"thus":[118],"suggest":[119],"that":[120],"engineering":[121],"retaining":[129],"could":[132],"be":[133],"general":[135],"way":[136],"turn":[138]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":2}],"updated_date":"2026-10-08T09:06:37.294493","created_date":"2025-10-10T00:00:00"}