{"id":"https://openalex.org/W2980050164","doi":null,"title":"Combining tissue engineering and gene delivery to enhance peripheral nerve regeneration","display_name":"Combining tissue engineering and gene delivery to enhance peripheral nerve regeneration","publication_year":2019,"publication_date":"2019-07-28","ids":{"openalex":"https://openalex.org/W2980050164","mag":"2980050164"},"language":"en","primary_location":{"id":"pmh:oai:eprints.ucl.ac.uk.OAI2:10077365","doi":null,"is_oa":false,"landing_page_url":"http://discovery.ucl.ac.uk/10077365/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400024","display_name":"UCL Discovery (University College London)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I45129253","host_organization_name":"University College London","host_organization_lineage":["https://openalex.org/I45129253"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Doctoral thesis, UCL (University College London).","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014016111","display_name":"Francesca Busuttil","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Francesca Busuttil","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5014016111"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10483","display_name":"Nerve injury and regeneration","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10483","display_name":"Nerve injury and regeneration","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12264","display_name":"Tissue Engineering and Regenerative Medicine","score":0.006399999838322401,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11601","display_name":"Neuroscience and Neural Engineering","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/peripheral-nerve-regeneration","display_name":"peripheral nerve regeneration","score":0.996999979019165},{"id":"https://openalex.org/keywords/vegf165","display_name":"VEGF165","score":0.9409999847412109},{"id":"https://openalex.org/keywords/schwann-cells","display_name":"Schwann cells","score":0.8690000176429749},{"id":"https://openalex.org/keywords/lentiviral-gene-delivery","display_name":"lentiviral gene delivery","score":0.890999972820282},{"id":"https://openalex.org/keywords/bioluminescence-imaging","display_name":"bioluminescence imaging","score":0.9179999828338623},{"id":"https://openalex.org/keywords/angiogenesis","display_name":"angiogenesis","score":0.7609999775886536},{"id":"https://openalex.org/keywords/endothelial-tube-formation","display_name":"endothelial tube formation","score":0.7749999761581421},{"id":"https://openalex.org/keywords/neural-stem-cells","display_name":"neural stem cells","score":0.8109999895095825},{"id":"https://openalex.org/keywords/adipose-derived-stem-cells","display_name":"adipose-derived stem cells","score":0.9490000009536743},{"id":"https://openalex.org/keywords/sciatic-nerve-injury","display_name":"sciatic nerve injury","score":0.9929999709129333},{"id":"https://openalex.org/keywords/axon-regeneration","display_name":"axon regeneration","score":0.5889999866485596},{"id":"https://openalex.org/keywords/functional-recovery","display_name":"functional recovery","score":0.4320000112056732}],"concepts":[{"id":"https://openalex.org/C161997846","wikidata":"https://www.wikidata.org/wiki/Q4915066","display_name":"Bioluminescence imaging","level":5,"score":0.666972279548645},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6048958897590637},{"id":"https://openalex.org/C171056886","wikidata":"https://www.wikidata.org/wiki/Q193119","display_name":"Regeneration (biology)","level":2,"score":0.5601276755332947},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.5407782196998596},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.43539226055145264},{"id":"https://openalex.org/C49892992","wikidata":"https://www.wikidata.org/wiki/Q1540285","display_name":"Tissue engineering","level":2,"score":0.4205150008201599},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3993165194988251},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.35814982652664185},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3505173623561859},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3346346914768219},{"id":"https://openalex.org/C111335760","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferase","level":4,"score":0.3262968063354492},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.3203859329223633},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.29099759459495544},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.1737518608570099},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.17267438769340515},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09047207236289978},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.06312161684036255},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:eprints.ucl.ac.uk.OAI2:10077365","doi":null,"is_oa":false,"landing_page_url":"http://discovery.ucl.ac.uk/10077365/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400024","display_name":"UCL Discovery (University College London)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I45129253","host_organization_name":"University College London","host_organization_lineage":["https://openalex.org/I45129253"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Doctoral thesis, UCL (University College London).","raw_type":"Thesis"},{"id":"mag:2980050164","doi":null,"is_oa":false,"landing_page_url":"https://discovery.ucl.ac.uk/id/eprint/10077365/","pdf_url":null,"source":{"id":"https://openalex.org/S4306508231","display_name":"Doctoral thesis, UCL (University College London).","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Doctoral thesis, UCL (University College London).","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9789000153541565,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":224,"referenced_works":["https://openalex.org/W27407830","https://openalex.org/W113485285","https://openalex.org/W148516797","https://openalex.org/W247697670","https://openalex.org/W265142245","https://openalex.org/W285486479","https://openalex.org/W318637039","https://openalex.org/W1527641279","https://openalex.org/W1531080825","https://openalex.org/W1535813017","https://openalex.org/W1556826327","https://openalex.org/W1582366896","https://openalex.org/W1582840909","https://openalex.org/W1596941966","https://openalex.org/W1624346216","https://openalex.org/W1655540037","https://openalex.org/W1701172542","https://openalex.org/W1769569140","https://openalex.org/W1825323076","https://openalex.org/W1865134586","https://openalex.org/W1885786751","https://openalex.org/W1916225419","https://openalex.org/W1922410518","https://openalex.org/W1935201742","https://openalex.org/W1962154683","https://openalex.org/W1963128268","https://openalex.org/W1963634791","https://openalex.org/W1965571714","https://openalex.org/W1966763767","https://openalex.org/W1966983163","https://openalex.org/W1969271703","https://openalex.org/W1969552240","https://openalex.org/W1970819272","https://openalex.org/W1971749062","https://openalex.org/W1973009368","https://openalex.org/W1975566751","https://openalex.org/W1975641354","https://openalex.org/W1976117064","https://openalex.org/W1976714932","https://openalex.org/W1979584236","https://openalex.org/W1981222676","https://openalex.org/W1982542580","https://openalex.org/W1985525878","https://openalex.org/W1985712533","https://openalex.org/W1986062352","https://openalex.org/W1986970772","https://openalex.org/W1988911750","https://openalex.org/W1990237501","https://openalex.org/W1991131268","https://openalex.org/W1991362893","https://openalex.org/W1991502989","https://openalex.org/W1991574099","https://openalex.org/W1992137840","https://openalex.org/W1992416092","https://openalex.org/W1993965974","https://openalex.org/W1994476469","https://openalex.org/W1995191245","https://openalex.org/W1995554653","https://openalex.org/W1995945082","https://openalex.org/W1996747774","https://openalex.org/W1998855928","https://openalex.org/W2000292756","https://openalex.org/W2002350491","https://openalex.org/W2002397995","https://openalex.org/W2003168311","https://openalex.org/W2004020292","https://openalex.org/W2004491277","https://openalex.org/W2004602806","https://openalex.org/W2006519535","https://openalex.org/W2006923247","https://openalex.org/W2008341781","https://openalex.org/W2016604988","https://openalex.org/W2017478350","https://openalex.org/W2019602403","https://openalex.org/W2021889176","https://openalex.org/W2022169914","https://openalex.org/W2022308075","https://openalex.org/W2023215998","https://openalex.org/W2024341039","https://openalex.org/W2025774463","https://openalex.org/W2027069301","https://openalex.org/W2027296526","https://openalex.org/W2028201091","https://openalex.org/W2029212111","https://openalex.org/W2030441392","https://openalex.org/W2031595735","https://openalex.org/W2031868434","https://openalex.org/W2032231759","https://openalex.org/W2033552852","https://openalex.org/W2033641981","https://openalex.org/W2038236689","https://openalex.org/W2038683096","https://openalex.org/W2038997019","https://openalex.org/W2039122179","https://openalex.org/W2041889817","https://openalex.org/W2042769704","https://openalex.org/W2042928696","https://openalex.org/W2042993639","https://openalex.org/W2043011805","https://openalex.org/W2044017153","https://openalex.org/W2044385536","https://openalex.org/W2044959577","https://openalex.org/W2045314069","https://openalex.org/W2046654995","https://openalex.org/W2046770473","https://openalex.org/W2046866438","https://openalex.org/W2048670135","https://openalex.org/W2048890215","https://openalex.org/W2052424792","https://openalex.org/W2053576250","https://openalex.org/W2054271984","https://openalex.org/W2054774435","https://openalex.org/W2056146626","https://openalex.org/W2059005277","https://openalex.org/W2061028146","https://openalex.org/W2061940955","https://openalex.org/W2064217264","https://openalex.org/W2064317688","https://openalex.org/W2065123875","https://openalex.org/W2065762680","https://openalex.org/W2068783587","https://openalex.org/W2069131272","https://openalex.org/W2070881126","https://openalex.org/W2071404720","https://openalex.org/W2072588717","https://openalex.org/W2072760341","https://openalex.org/W2073039966","https://openalex.org/W2077386069","https://openalex.org/W2078014172","https://openalex.org/W2079273552","https://openalex.org/W2080468808","https://openalex.org/W2080586755","https://openalex.org/W2081144380","https://openalex.org/W2081373880","https://openalex.org/W2081492581","https://openalex.org/W2083178131","https://openalex.org/W2083737682","https://openalex.org/W2084029306","https://openalex.org/W2086724431","https://openalex.org/W2087508782","https://openalex.org/W2089319449","https://openalex.org/W2089579355","https://openalex.org/W2089720406","https://openalex.org/W2091562888","https://openalex.org/W2091767912","https://openalex.org/W2094307792","https://openalex.org/W2094988427","https://openalex.org/W2098532127","https://openalex.org/W2100938404","https://openalex.org/W2101099639","https://openalex.org/W2103286425","https://openalex.org/W2109378266","https://openalex.org/W2112516900","https://openalex.org/W2113051470","https://openalex.org/W2115590226","https://openalex.org/W2115893258","https://openalex.org/W2116082618","https://openalex.org/W2116087648","https://openalex.org/W2125540465","https://openalex.org/W2128094872","https://openalex.org/W2128377922","https://openalex.org/W2129431919","https://openalex.org/W2138224666","https://openalex.org/W2138784256","https://openalex.org/W2139028184","https://openalex.org/W2141040794","https://openalex.org/W2141570423","https://openalex.org/W2141861652","https://openalex.org/W2142478822","https://openalex.org/W2144480794","https://openalex.org/W2144966500","https://openalex.org/W2145769191","https://openalex.org/W2146276847","https://openalex.org/W2147599447","https://openalex.org/W2147813206","https://openalex.org/W2148897921","https://openalex.org/W2149158668","https://openalex.org/W2154461811","https://openalex.org/W2154853638","https://openalex.org/W2155103162","https://openalex.org/W2156032975","https://openalex.org/W2159530833","https://openalex.org/W2166920703","https://openalex.org/W2167523976","https://openalex.org/W2256588434","https://openalex.org/W2278275104","https://openalex.org/W2289757141","https://openalex.org/W2313862609","https://openalex.org/W2319562462","https://openalex.org/W2322025889","https://openalex.org/W2328140935","https://openalex.org/W2331628193","https://openalex.org/W2344661736","https://openalex.org/W2396814975","https://openalex.org/W2399789944","https://openalex.org/W2433239291","https://openalex.org/W2461654568","https://openalex.org/W2471856832","https://openalex.org/W2505538944","https://openalex.org/W2514630864","https://openalex.org/W2527204490","https://openalex.org/W2539312529","https://openalex.org/W2582407774","https://openalex.org/W2582965812","https://openalex.org/W2601251566","https://openalex.org/W2601685387","https://openalex.org/W2603555009","https://openalex.org/W2603637209","https://openalex.org/W2612208891","https://openalex.org/W2615853094","https://openalex.org/W2754843959","https://openalex.org/W2760846415","https://openalex.org/W2766800689","https://openalex.org/W2776985607","https://openalex.org/W2788051494","https://openalex.org/W2789736716","https://openalex.org/W2791408070","https://openalex.org/W2793837622","https://openalex.org/W2795177441","https://openalex.org/W2795363800","https://openalex.org/W2801522153","https://openalex.org/W2811154491","https://openalex.org/W2987151899","https://openalex.org/W3025501632"],"related_works":["https://openalex.org/W1985261667","https://openalex.org/W1993557866","https://openalex.org/W2288935836","https://openalex.org/W4393340578","https://openalex.org/W2110711342","https://openalex.org/W987594621","https://openalex.org/W2034700594","https://openalex.org/W2136807038","https://openalex.org/W2773481440","https://openalex.org/W2052028793"],"abstract_inverted_index":{"There":[0],"has":[1],"been":[2],"an":[3,122],"increased":[4,170,182,273],"interest":[5],"in":[6,42,86,99,112,127,135,178,203,232,268],"the":[7,87,93,96,100,110,113,155,166,224,231,295],"development":[8],"of":[9,22,95,109,124,251,299,301],"nerve":[10,16,29,253],"repair":[11,30],"devices":[12,31],"to":[13,26,49,56,72,82,131,148,194,280,291,294,303],"improve":[14],"peripheral":[15],"regeneration":[17],"following":[18],"injury.":[19],"The":[20,67,162,282],"aim":[21],"this":[23,264,285],"study":[24,45,261,286],"was":[25,46,80,90,158,236],"investigate":[27],"whether":[28],"containing":[32],"genetically":[33],"modified":[34],"cells":[35,69,111,145,169,189,212,218,243],"overexpressing":[36,190,244],"VEGF-A165":[37,156,163,191,245,302],"would":[38,103,255],"augment":[39],"regeneration.":[40,257],"An":[41],"vitro":[43,233],"proof-of-concept":[44],"carried":[47],"out":[48],"deliver":[50],"marker":[51],"genes":[52],"(luciferase":[53],"and":[54,77,106,137,160,175,186,200,213,275,297,308],"eGFP)":[55],"a":[57,64,151,248,259],"rat":[58,214,249],"Schwann":[59],"cell":[60,84,125,172,184,197],"line":[61],"(SCL4.1/F7)":[62],"using":[63],"lentiviral":[65,149,152,225],"vector.":[66],"transduced":[68,167,222],"were":[70,146,192,219],"used":[71,81],"produce":[73],"engineered":[74,114],"neural":[75,115,210],"tissue":[76],"bioluminescence":[78,119],"imaging":[79,108,120],"assess":[83],"viability":[85,126],"constructs.":[88],"It":[89,180],"initially":[91],"thought":[92],"presence":[94],"luciferase":[97],"gene":[98,157],"expression":[101,300],"cassette":[102],"allow":[104],"real-time":[105],"sustained":[107],"tissue.":[116],"However,":[117],"while":[118],"provided":[121],"indication":[123],"vitro,":[128],"it":[129,235],"proved":[130],"be":[132,292],"ineffective":[133],"for":[134],"vivo":[136,139],"ex":[138],"imaging.":[140],"Having":[141],"established":[142],"that":[143,238,263,288],"SCL4.1/F7":[144,168,183,188,242],"amenable":[147],"transduction,":[150],"vector":[153,226],"delivering":[154,227],"designed":[159],"produced.":[161],"produced":[164],"by":[165],"endothelial":[171,196],"viability,":[173],"migration":[174],"tube":[176],"formation":[177,199],"vitro.":[179],"also":[181,220],"proliferation":[185],"migration.":[187],"found":[193],"increase":[195],"network":[198],"neurite":[201],"length":[202],"3D":[204],"co-":[205],"culture":[206],"models.":[207],"Foetal":[208],"human":[209],"stem":[211,217],"adipose":[215],"derived":[216],"successfully":[221],"with":[223],"VEGF-A165.":[228],"Based":[229],"on":[230],"results,":[234],"postulated":[237],"EngNT":[239],"made":[240],"from":[241,284],"implanted":[246],"into":[247],"model":[250],"sciatic":[252],"injury":[254],"enhance":[256],"Unexpectedly,":[258],"pilot":[260],"revealed":[262],"did":[265],"not":[266],"result":[267],"improved":[269],"functional":[270],"recovery":[271],"or":[272],"axon":[274],"blood":[276],"vessel":[277],"counts":[278],"compared":[279],"controls.":[281],"results":[283],"highlight":[287],"attention":[289],"needs":[290],"paid":[293],"dose":[296],"duration":[298],"optimise":[304],"both":[305],"its":[306],"angiogenic":[307],"neurotrophic":[309],"effects.":[310]},"counts_by_year":[],"updated_date":"2025-10-10T17:16:08.811792","created_date":"2019-10-18T00:00:00"}