{"id":"https://openalex.org/W2979609592","doi":null,"title":"Effects of Temperature on the Proliferation of Human and Fish Lens Epithelial Cells","display_name":"Effects of Temperature on the Proliferation of Human and Fish Lens Epithelial Cells","publication_year":2019,"publication_date":"2019-08-29","ids":{"openalex":"https://openalex.org/W2979609592","mag":"2979609592"},"language":"en","primary_location":{"id":"pmh:oai:uwspace.uwaterloo.ca:10012/14997","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10012/14997","pdf_url":"http://hdl.handle.net/10012/14997","source":{"id":"https://openalex.org/S4306401661","display_name":"UWSpace (University of Waterloo)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I151746483","host_organization_name":"University of Waterloo","host_organization_lineage":["https://openalex.org/I151746483"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Master Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10012/14997","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101596438","display_name":"Ziqing Li","orcid":"https://orcid.org/0000-0003-3596-3520","observed_orcids":["https://orcid.org/0000-0003-3596-3520"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ziqing Li","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-3596-3520","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5101596438"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.3073999881744385,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10934","display_name":"Heat shock proteins research","score":0.3073999881744385,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11480","display_name":"Connexins and lens biology","score":0.18369999527931213,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11056","display_name":"Physiological and biochemical adaptations","score":0.15389999747276306,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lens-epithelial-cells","display_name":"lens epithelial cells","score":0.9769999980926514},{"id":"https://openalex.org/keywords/temperature-effects","display_name":"temperature effects","score":0.8500000238418579},{"id":"https://openalex.org/keywords/cell-proliferation","display_name":"cell proliferation","score":0.9580000042915344},{"id":"https://openalex.org/keywords/crystallins","display_name":"crystallins","score":0.6169999837875366},{"id":"https://openalex.org/keywords/alpha-crystallin","display_name":"alpha-crystallin","score":0.9350000023841858},{"id":"https://openalex.org/keywords/gamma-crystallin","display_name":"gamma-crystallin","score":0.9860000014305115},{"id":"https://openalex.org/keywords/heat-shock-proteins","display_name":"heat shock proteins","score":0.7990000247955322},{"id":"https://openalex.org/keywords/cryoproteins","display_name":"cryoproteins","score":0.871999979019165},{"id":"https://openalex.org/keywords/rainbow-trout","display_name":"rainbow trout","score":0.8009999990463257},{"id":"https://openalex.org/keywords/human-lens","display_name":"human lens","score":0.8230000138282776},{"id":"https://openalex.org/keywords/cell-clumping","display_name":"cell clumping","score":0.671999990940094},{"id":"https://openalex.org/keywords/cellular-distribution","display_name":"cell distribution","score":0.6499999761581421},{"id":"https://openalex.org/keywords/cell-size","display_name":"cell size","score":0.40299999713897705},{"id":"https://openalex.org/keywords/mitotic-rate","display_name":"mitotic activity","score":0.3720000088214874}],"concepts":[{"id":"https://openalex.org/C2909208804","wikidata":"https://www.wikidata.org/wiki/Q127282","display_name":"Fish <Actinopterygii>","level":2,"score":0.6395357847213745},{"id":"https://openalex.org/C15336307","wikidata":"https://www.wikidata.org/wiki/Q1766051","display_name":"Lens (geology)","level":2,"score":0.6079875826835632},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3928011953830719},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3661051392555237},{"id":"https://openalex.org/C505870484","wikidata":"https://www.wikidata.org/wiki/Q180538","display_name":"Fishery","level":1,"score":0.31033197045326233},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:uwspace.uwaterloo.ca:10012/14997","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10012/14997","pdf_url":"http://hdl.handle.net/10012/14997","source":{"id":"https://openalex.org/S4306401661","display_name":"UWSpace (University of Waterloo)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I151746483","host_organization_name":"University of Waterloo","host_organization_lineage":["https://openalex.org/I151746483"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Master Thesis"},{"id":"mag:2979609592","doi":null,"is_oa":false,"landing_page_url":"https://uwspace.uwaterloo.ca/handle/10012/14997","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:uwspace.uwaterloo.ca:10012/14997","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10012/14997","pdf_url":"http://hdl.handle.net/10012/14997","source":{"id":"https://openalex.org/S4306401661","display_name":"UWSpace (University of Waterloo)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I151746483","host_organization_name":"University of Waterloo","host_organization_lineage":["https://openalex.org/I151746483"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Master Thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2979609592.pdf","grobid_xml":"https://content.openalex.org/works/W2979609592.grobid-xml"},"referenced_works_count":69,"referenced_works":["https://openalex.org/W29616887","https://openalex.org/W157534170","https://openalex.org/W374075938","https://openalex.org/W857701621","https://openalex.org/W1168761028","https://openalex.org/W1483513243","https://openalex.org/W1485026392","https://openalex.org/W1496792644","https://openalex.org/W1577503218","https://openalex.org/W1594197304","https://openalex.org/W1734427958","https://openalex.org/W1869667145","https://openalex.org/W1964398779","https://openalex.org/W1964959216","https://openalex.org/W1971903586","https://openalex.org/W1975931443","https://openalex.org/W1976845238","https://openalex.org/W1985139403","https://openalex.org/W1986422592","https://openalex.org/W1989052059","https://openalex.org/W1991198899","https://openalex.org/W1996485878","https://openalex.org/W2004307230","https://openalex.org/W2009147159","https://openalex.org/W2016230118","https://openalex.org/W2016492849","https://openalex.org/W2022873399","https://openalex.org/W2027101632","https://openalex.org/W2031307848","https://openalex.org/W2031377249","https://openalex.org/W2038218463","https://openalex.org/W2042013946","https://openalex.org/W2045222844","https://openalex.org/W2045252383","https://openalex.org/W2047651912","https://openalex.org/W2049689878","https://openalex.org/W2051424632","https://openalex.org/W2057252841","https://openalex.org/W2057592454","https://openalex.org/W2059866896","https://openalex.org/W2060174041","https://openalex.org/W2068186398","https://openalex.org/W2071421762","https://openalex.org/W2075218366","https://openalex.org/W2086434199","https://openalex.org/W2087959155","https://openalex.org/W2095365065","https://openalex.org/W2099510413","https://openalex.org/W2111420633","https://openalex.org/W2122211002","https://openalex.org/W2124664315","https://openalex.org/W2129785995","https://openalex.org/W2139298045","https://openalex.org/W2139647802","https://openalex.org/W2148837554","https://openalex.org/W2152641758","https://openalex.org/W2165958384","https://openalex.org/W2244608638","https://openalex.org/W2588902758","https://openalex.org/W2792074162","https://openalex.org/W2792120874","https://openalex.org/W2796279370","https://openalex.org/W2944506567","https://openalex.org/W3013967509","https://openalex.org/W3023394642","https://openalex.org/W4206326271","https://openalex.org/W4210370525","https://openalex.org/W4249029242","https://openalex.org/W4251099864"],"related_works":["https://openalex.org/W2007892210","https://openalex.org/W2531554793","https://openalex.org/W3112877328","https://openalex.org/W2154160468","https://openalex.org/W2054338188","https://openalex.org/W2963235687","https://openalex.org/W858067751","https://openalex.org/W2007295851","https://openalex.org/W2013410907","https://openalex.org/W2059866896"],"abstract_inverted_index":{"Crystallins":[0,24],"are":[1,43],"proteins":[2],"that":[3,74],"confer":[4],"refractive":[5],"properties":[6],"to":[7,45,173,218,242,258],"the":[8,67,98,136,174,200,225,250,263],"crystalline":[9],"lens.":[10],"All":[11],"vertebrate":[12],"lenses":[13,37,49,61,76,86],"contain":[14],"\u03b1-":[15],"and":[16,18,41,84,108,113,124,132,139,146,149,155,163,183],"\u03b2-crystallins,":[17],"often":[19],"a":[20,239,243,256,259],"third":[21],"major":[22],"crystallin.":[23],"can":[25],"have":[26],"additional":[27],"non-refractive":[28],"functions;":[29],"\u03b1-crystallins":[30,56],"act":[31],"as":[32],"heat":[33],"shock":[34],"proteins,":[35],"protecting":[36,48],"from":[38,50,106,238,255],"heat-induced":[39],"denaturation,":[40],"\u03b3-crystallins":[42],"thought":[44],"be":[46,78],"cryoproteins,":[47],"extreme":[51],"cold.":[52],"The":[53,197],"concentration":[54],"of":[55,100,199,267],"is":[57,69],"higher":[58,142],"in":[59,63,81,89,190],"mammalian":[60,75],"than":[62,143,151,177],"teleost":[64,85],"lenses,":[65],"while":[66],"opposite":[68,251],"true":[70],"for":[71,126,180],"\u03b3-crystallins,":[72],"suggesting":[73],"would":[77],"better":[79,87],"protected":[80,88],"warmer":[82],"conditions":[83],"colder":[90],"temperatures.":[91,196],"This":[92],"study":[93],"determined":[94],"whether":[95],"temperature":[96,207,216,227,265],"affects":[97],"growth":[99],"lens":[101],"epithelial":[102],"cells":[103,187,201,213],"(LECs)":[104],"derived":[105],"human":[107,112,164,182,212],"fish":[109,116,162,184,191,268],"lenses.":[110],"Both":[111],"rainbow":[114],"trout":[115],"LECs":[117,165,192,223,233,253,269],"were":[118,170,188],"cultured":[119],"(n":[120],"=":[121],"4":[122],"each)":[123],"grown":[125,193],"1,":[127],"2,":[128],"4,":[129],"6,":[130],"8":[131],"12":[133],"days":[134],"at":[135,194,214,224,234],"optimal":[137,144,152,159,226,235],"(37\u00b0C":[138],"18\u00b0C,":[140],"respectively),":[141],"(42\u00b0C":[145],"25\u00b0C,":[147],"respectively)":[148],"lower":[150,178,247],"temperatures":[153,169,179,236,248],"(32\u00b0C":[154],"10\u00b0C,":[156],"respectively).":[157],"At":[158],"temperatures,":[160],"both":[161,181],"grew":[166],"optimally.":[167],"Higher":[168],"more":[171],"deleterious":[172],"proliferation":[175],"index":[176],"LECs.":[185],"Mitotic":[186],"non-existent":[189],"high":[195,264],"sizes":[198],"did":[202],"not":[203],"greatly":[204],"change":[205],"with":[206,208],"either":[209],"species,":[210],"but":[211,246],"non-optimal":[215],"tended":[217],"clump":[219],"over":[220],"time.":[221],"Human":[222],"maintained":[228],"their":[229,271],"random":[230,240,260,272],"distribution.":[231,261],"Fish":[232],"moved":[237,254],"distribution":[241],"clumped":[244,257],"distribution,":[245],"had":[249],"effect;":[252],"Only":[262],"group":[266],"maintain":[270],"organisation.":[273]},"counts_by_year":[{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2019-10-18T00:00:00"}