{"id":"https://openalex.org/W2978289313","doi":"https://doi.org/10.21161/mjm.190444","title":"Cloning and expression of hyaluronan synthase (hasA) in recombinant Escherichia coli BL21 and its hyaluronic acid production in shake flask culture","display_name":"Cloning and expression of hyaluronan synthase (hasA) in recombinant Escherichia coli BL21 and its hyaluronic acid production in shake flask culture","publication_year":2019,"publication_date":"2019-10-03","ids":{"openalex":"https://openalex.org/W2978289313","doi":"https://doi.org/10.21161/mjm.190444","mag":"2978289313"},"language":"en","primary_location":{"id":"doi:10.21161/mjm.190444","doi":"https://doi.org/10.21161/mjm.190444","is_oa":true,"landing_page_url":"https://doi.org/10.21161/mjm.190444","pdf_url":"http://mjm.usm.my/uploads/issues/1563/MJM-19-0444_COLOUR.pdf","source":{"id":"https://openalex.org/S2738051874","display_name":"Malaysian Journal of Microbiology","issn_l":"2231-7538","issn":["2231-7538","1823-8262"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","poland-20","russia-white-list-4"],"host_organization":"https://openalex.org/P4310320911","host_organization_name":"Malaysian Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320911"],"host_organization_lineage_names":["Malaysian Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Malaysian Journal of Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"http://mjm.usm.my/uploads/issues/1563/MJM-19-0444_COLOUR.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014670272","display_name":"Zee Wei Lai","orcid":"https://orcid.org/0000-0002-8521-4686","observed_orcids":["https://orcid.org/0000-0002-8521-4686"]},"institutions":[{"id":"https://openalex.org/I4210143550","display_name":"Taylor's University","ror":"https://ror.org/0498pcx51","country_code":"MY","type":"education","lineage":["https://openalex.org/I4210143550"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Lai, Z. W.","raw_affiliation_strings":["School of Biosciences , Faculty of Health and Medical Sciences , Taylor's University Lakeside Campus , No. 1 , Jalan Taylors , 47500 Subang Jaya , Selangor Darul Ehsan , Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biosciences , Faculty of Health and Medical Sciences , Taylor's University Lakeside Campus , No. 1 , Jalan Taylors , 47500 Subang Jaya , Selangor Darul Ehsan , Malaysia","institution_ids":["https://openalex.org/I4210143550"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055247947","display_name":"Chee How Teo","orcid":"https://orcid.org/0000-0002-8118-2592","observed_orcids":["https://orcid.org/0000-0002-8118-2592"]},"institutions":[{"id":"https://openalex.org/I33849332","display_name":"University of Malaya","ror":"https://ror.org/00rzspn62","country_code":"MY","type":"education","lineage":["https://openalex.org/I33849332"]}],"countries":["MY"],"is_corresponding":false,"raw_author_name":"Teo, C. H.","raw_affiliation_strings":["Center for Research in Biotechnology for Agriculture , Level 3 , Research Management and Innovation Complex , University of Malaya , Lembah Pantai , 50603 Kuala Lumpur , Malaysia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Research in Biotechnology for Agriculture , Level 3 , Research Management and Innovation Complex , University of Malaya , Lembah Pantai , 50603 Kuala Lumpur , Malaysia","institution_ids":["https://openalex.org/I33849332"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":110,"currency":"USD","value_usd":110},"apc_paid":{"value":110,"currency":"USD","value_usd":110},"fwci":0.1805,"has_fulltext":true,"cited_by_count":10,"citation_normalized_percentile":{"value":0.39656696,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.8485999703407288,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":0.8485999703407288,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12192","display_name":"Polysaccharides Composition and Applications","score":0.10080000013113022,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.013000000268220901,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hyaluronic-acid","display_name":"hyaluronic acid","score":0.8650000095367432},{"id":"https://openalex.org/keywords/hyaluronan-synthase","display_name":"hyaluronan synthase","score":0.9710000157356262},{"id":"https://openalex.org/keywords/streptococcus-zooepidemicus","display_name":"Streptococcus zooepidemicus","score":0.9549999833106995},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.8629999756813049},{"id":"https://openalex.org/keywords/iptg-induction","display_name":"IPTG induction","score":0.8529999852180481},{"id":"https://openalex.org/keywords/glucose-levels","display_name":"glucose concentration","score":0.9480000138282776},{"id":"https://openalex.org/keywords/fermentation-medium-optimization","display_name":"fermentation medium optimization","score":0.675000011920929},{"id":"https://openalex.org/keywords/low-molecular-weight-hyaluronic-acid","display_name":"low molecular weight hyaluronic acid","score":0.7879999876022339},{"id":"https://openalex.org/keywords/antioxidant-activity","display_name":"antioxidant activity","score":0.5509999990463257}],"concepts":[{"id":"https://openalex.org/C2777529286","wikidata":"https://www.wikidata.org/wiki/Q337231","display_name":"Hyaluronic acid","level":2,"score":0.8319175243377686},{"id":"https://openalex.org/C2781236742","wikidata":"https://www.wikidata.org/wiki/Q5952725","display_name":"Hyaluronan synthase","level":3,"score":0.810245156288147},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.7663087844848633},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.7500431537628174},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6958361864089966},{"id":"https://openalex.org/C2779053110","wikidata":"https://www.wikidata.org/wiki/Q7462601","display_name":"Shake","level":2,"score":0.565117359161377},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4976063072681427},{"id":"https://openalex.org/C112243037","wikidata":"https://www.wikidata.org/wiki/Q22327117","display_name":"ATP synthase","level":3,"score":0.44658005237579346},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.42528074979782104},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3743554949760437},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3278103470802307},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.30579957365989685},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.21387749910354614},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.15761488676071167},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11768454313278198},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C1276947","wikidata":"https://www.wikidata.org/wiki/Q333","display_name":"Astronomy","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21161/mjm.190444","doi":"https://doi.org/10.21161/mjm.190444","is_oa":true,"landing_page_url":"https://doi.org/10.21161/mjm.190444","pdf_url":"http://mjm.usm.my/uploads/issues/1563/MJM-19-0444_COLOUR.pdf","source":{"id":"https://openalex.org/S2738051874","display_name":"Malaysian Journal of Microbiology","issn_l":"2231-7538","issn":["2231-7538","1823-8262"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","poland-20","russia-white-list-4"],"host_organization":"https://openalex.org/P4310320911","host_organization_name":"Malaysian Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320911"],"host_organization_lineage_names":["Malaysian Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Malaysian Journal of Microbiology","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.21161/mjm.190444","doi":"https://doi.org/10.21161/mjm.190444","is_oa":true,"landing_page_url":"https://doi.org/10.21161/mjm.190444","pdf_url":"http://mjm.usm.my/uploads/issues/1563/MJM-19-0444_COLOUR.pdf","source":{"id":"https://openalex.org/S2738051874","display_name":"Malaysian Journal of Microbiology","issn_l":"2231-7538","issn":["2231-7538","1823-8262"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","poland-20","russia-white-list-4"],"host_organization":"https://openalex.org/P4310320911","host_organization_name":"Malaysian Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320911"],"host_organization_lineage_names":["Malaysian Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Malaysian Journal of Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4043000042438507,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2978289313.pdf","grobid_xml":"https://content.openalex.org/works/W2978289313.grobid-xml"},"referenced_works_count":16,"referenced_works":["https://openalex.org/W1986723193","https://openalex.org/W1999652145","https://openalex.org/W2013941038","https://openalex.org/W2015349683","https://openalex.org/W2031763175","https://openalex.org/W2034008313","https://openalex.org/W2036938464","https://openalex.org/W2049399739","https://openalex.org/W2062608769","https://openalex.org/W2070528883","https://openalex.org/W2070774028","https://openalex.org/W2076018628","https://openalex.org/W2085742315","https://openalex.org/W2102267945","https://openalex.org/W2154979806","https://openalex.org/W2561658694"],"related_works":["https://openalex.org/W2612644771","https://openalex.org/W2051721236","https://openalex.org/W2594133945","https://openalex.org/W4310205498","https://openalex.org/W7127171270","https://openalex.org/W4285088963","https://openalex.org/W4408449947","https://openalex.org/W2119044039","https://openalex.org/W2371354668","https://openalex.org/W2034008313"],"abstract_inverted_index":{"Aims:":[0],"Hyaluronic":[1],"acid":[2],"(HA)":[3],"is":[4,31,207,241],"a":[5,11,200],"high":[6],"molecular":[7,238],"weight":[8,239],"polymer":[9],"and":[10,19,36,48,71,80,120,151,157,216,228,247,257],"major":[12],"component":[13],"of":[14,23,40,65,73,84,88,141,193,218],"mucoid":[15],"capsule":[16],"in":[17,34,68,160,231,245],"bacteria":[18],"extracellular":[20],"matrix":[21],"(ECM)":[22],"vertebrate":[24],"tissue.Due":[25],"to":[26,182,212,251],"its":[27],"unique":[28],"characteristics,":[29],"HA":[30,51,78,142,186,197,240],"used":[32,137],"extensively":[33],"medical":[35,246],"cosmetic":[37,248],"field.However,":[38],"because":[39],"the":[41,74,93,115,123,139,184],"exotoxins":[42],"production":[43,52,140,198],"from":[44,98,225],"animal":[45],"tissues":[46],"extraction":[47],"Streptococcus":[49],"zooepidemicus,":[50],"by":[53,105,143],"recombinant":[54,232],"microorganisms":[55],"has":[56],"gained":[57],"interest.The":[58],"present":[59],"study":[60],"was":[61,103,112,136,180,223],"aimed":[62],"at":[63],"cloning":[64],"hasA":[66,110,221],"gene":[67,97,111,222],"Escherichia":[69,126],"coli":[70,127,133,234],"optimization":[72],"medium":[75],"components":[76],"for":[77,138],"production.Methodology":[79],"results:":[81],"A":[82],"fragment":[83],"an":[85],"approximate":[86],"size":[87],"1.5":[89],"kb":[90],"that":[91,169],"encodes":[92],"hyaluronan":[94],"synthase":[95],"(hasA)":[96],"S.":[99,226],"zooepidemicus":[100,227],"ATCC":[101],"39920":[102],"amplified":[104],"PCR":[106],"using":[107,145],"hasA-specific":[108],"primers.The":[109],"ligated":[113],"into":[114,122],"bacterial":[116],"expression":[117,124],"vector":[118],"pLbADH":[119],"transformed":[121],"host,":[125],"BL21":[128,135,235],"strain.Then,":[129],"genetically":[130],"engineered":[131],"E.":[132,233],"strain":[134],"fermentation":[144],"different":[146,152],"glucose":[147,165,172],"concentration":[148,154,187],"(10-50":[149],"g/L)":[150],"IPTG":[153],"(0.1,":[155],"0.5":[156],"1.0":[158,194],"mM)":[159],"shake":[161],"flask":[162],"culture.Amongst":[163],"varying":[164],"concentrations,":[166],"results":[167],"showed":[168],"50":[170],"g/L":[171,205],"with":[173],"nutrient":[174],"rich":[175],"media":[176],"containing":[177],"nitrogen":[178],"source":[179],"able":[181],"produce":[183],"highest":[185],"(0.115":[188],"\u00b1":[189,203],"0.002":[190],"g/L).With":[191],"addition":[192],"mM":[195],"IPTG,":[196],"reached":[199],"peak":[201],"0.532":[202],"0.026":[204],"which":[206],"around":[208],"fivefold":[209],"higher":[210],"compared":[211],"without":[213],"IPTG.Conclusion,":[214],"significance":[215],"impact":[217],"study:":[219],"The":[220],"cloned":[224],"successfully":[229],"expressed":[230],"cells.This":[236],"low":[237],"gaining":[242],"more":[243],"importance":[244],"application":[249],"due":[250],"possess":[252],"pronounced":[253],"free":[254],"radical":[255],"scavenging":[256],"antioxidant":[258],"activities.":[259]},"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2019-10-10T00:00:00"}
