{"id":"https://openalex.org/W2970972134","doi":null,"title":"Selenium vs. Sulfur: Investigating the Substrate Specificity of a Selenocysteine Lyase","display_name":"Selenium vs. Sulfur: Investigating the Substrate Specificity of a Selenocysteine Lyase","publication_year":2019,"publication_date":"2019-01-01","ids":{"openalex":"https://openalex.org/W2970972134","mag":"2970972134"},"language":"en","primary_location":{"id":"pmh:oai:stars.library.ucf.edu:etd-7511","doi":null,"is_oa":true,"landing_page_url":"https://stars.library.ucf.edu/etd/6511","pdf_url":"https://stars.library.ucf.edu/etd/6511","source":{"id":"https://openalex.org/S4210172555","display_name":"Journal of International Crisis and Risk Communication Research","issn_l":"2576-0017","issn":["2576-0017","2576-0025"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","vabb-shw"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Theses and Dissertations","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://stars.library.ucf.edu/etd/6511","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Michael Johnstone","orcid":"https://orcid.org/0000-0002-8986-7086","observed_orcids":["https://orcid.org/0000-0002-8986-7086"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Michael Johnstone","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-8986-7086","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.13165925,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11107","display_name":"Selenium in Biological Systems","score":0.8428000211715698,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11107","display_name":"Selenium in Biological Systems","score":0.8428000211715698,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11931","display_name":"Organoselenium and organotellurium chemistry","score":0.15440000593662262,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/selenocysteine-lyase","display_name":"selenocysteine lyase","score":0.9919999837875366},{"id":"https://openalex.org/keywords/selenocysteine","display_name":"selenocysteine","score":0.8299999833106995},{"id":"https://openalex.org/keywords/cysteine","display_name":"cysteine","score":0.8579999804496765},{"id":"https://openalex.org/keywords/enterococcus-faecalis","display_name":"Enterococcus faecalis","score":0.8700000047683716},{"id":"https://openalex.org/keywords/cysteine-desulfurase","display_name":"cysteine desulfurase","score":0.8019999861717224},{"id":"https://openalex.org/keywords/competitive-inhibition","display_name":"competitive inhibition","score":0.5950000286102295},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.8529999852180481},{"id":"https://openalex.org/keywords/selenium-metabolism","display_name":"selenium metabolism","score":0.5540000200271606},{"id":"https://openalex.org/keywords/selenium-toxicity","display_name":"selenium toxicity","score":0.6539999842643738},{"id":"https://openalex.org/keywords/selenoproteins","display_name":"selenoproteins","score":0.6669999957084656},{"id":"https://openalex.org/keywords/nosocomial-pathogens","display_name":"nosocomial pathogens","score":0.6620000004768372}],"concepts":[{"id":"https://openalex.org/C2776087148","wikidata":"https://www.wikidata.org/wiki/Q408663","display_name":"Selenocysteine","level":4,"score":0.9634032845497131},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.730811357498169},{"id":"https://openalex.org/C553756173","wikidata":"https://www.wikidata.org/wiki/Q876","display_name":"Selenium","level":2,"score":0.7009939551353455},{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.6494038105010986},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5204691886901855},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5049079060554504},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.47682660818099976},{"id":"https://openalex.org/C2781139505","wikidata":"https://www.wikidata.org/wiki/Q5201195","display_name":"Cysteine metabolism","level":4,"score":0.43718913197517395},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4336720407009125},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.4291256368160248},{"id":"https://openalex.org/C2776841403","wikidata":"https://www.wikidata.org/wiki/Q2745644","display_name":"Selenoprotein","level":5,"score":0.4129818379878998},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.27920106053352356},{"id":"https://openalex.org/C538909803","wikidata":"https://www.wikidata.org/wiki/Q116907","display_name":"Glutathione","level":3,"score":0.14671853184700012},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C2778760513","wikidata":"https://www.wikidata.org/wiki/Q24773045","display_name":"Glutathione peroxidase","level":4,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:stars.library.ucf.edu:etd-7511","doi":null,"is_oa":true,"landing_page_url":"https://stars.library.ucf.edu/etd/6511","pdf_url":"https://stars.library.ucf.edu/etd/6511","source":{"id":"https://openalex.org/S4210172555","display_name":"Journal of International Crisis and Risk Communication Research","issn_l":"2576-0017","issn":["2576-0017","2576-0025"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","vabb-shw"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Theses and Dissertations","raw_type":"text"},{"id":"mag:2970972134","doi":null,"is_oa":false,"landing_page_url":"https://stars.library.ucf.edu/cgi/viewcontent.cgi?article=7511&context=etd","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:stars.library.ucf.edu:etd-7511","doi":null,"is_oa":true,"landing_page_url":"https://stars.library.ucf.edu/etd/6511","pdf_url":"https://stars.library.ucf.edu/etd/6511","source":{"id":"https://openalex.org/S4210172555","display_name":"Journal of International Crisis and Risk Communication Research","issn_l":"2576-0017","issn":["2576-0017","2576-0025"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","vabb-shw"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Electronic Theses and Dissertations","raw_type":"text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2970972134.pdf","grobid_xml":"https://content.openalex.org/works/W2970972134.grobid-xml"},"referenced_works_count":72,"referenced_works":["https://openalex.org/W39152875","https://openalex.org/W255996521","https://openalex.org/W1481775159","https://openalex.org/W1486135496","https://openalex.org/W1513577066","https://openalex.org/W1521628010","https://openalex.org/W1532421291","https://openalex.org/W1536886974","https://openalex.org/W1550873995","https://openalex.org/W1573792528","https://openalex.org/W1647577587","https://openalex.org/W1733622094","https://openalex.org/W1943327251","https://openalex.org/W1966386635","https://openalex.org/W1967483524","https://openalex.org/W1969391229","https://openalex.org/W1973334423","https://openalex.org/W1975920595","https://openalex.org/W1976619439","https://openalex.org/W1977268489","https://openalex.org/W1979918742","https://openalex.org/W1981139948","https://openalex.org/W1984610576","https://openalex.org/W1985844569","https://openalex.org/W2000682855","https://openalex.org/W2002207630","https://openalex.org/W2002209113","https://openalex.org/W2005305016","https://openalex.org/W2008991087","https://openalex.org/W2010018808","https://openalex.org/W2013202022","https://openalex.org/W2013206300","https://openalex.org/W2024152854","https://openalex.org/W2025354840","https://openalex.org/W2025919734","https://openalex.org/W2032929186","https://openalex.org/W2033955364","https://openalex.org/W2034599800","https://openalex.org/W2034672527","https://openalex.org/W2050599642","https://openalex.org/W2052616068","https://openalex.org/W2053280217","https://openalex.org/W2057168974","https://openalex.org/W2060121377","https://openalex.org/W2062424661","https://openalex.org/W2064373917","https://openalex.org/W2066198366","https://openalex.org/W2067781507","https://openalex.org/W2077770481","https://openalex.org/W2078419725","https://openalex.org/W2079712983","https://openalex.org/W2081201214","https://openalex.org/W2081682798","https://openalex.org/W2084004698","https://openalex.org/W2085765714","https://openalex.org/W2086805714","https://openalex.org/W2087921651","https://openalex.org/W2095534022","https://openalex.org/W2100837269","https://openalex.org/W2111436789","https://openalex.org/W2127322768","https://openalex.org/W2128360087","https://openalex.org/W2128635872","https://openalex.org/W2133705298","https://openalex.org/W2134503580","https://openalex.org/W2137557143","https://openalex.org/W2147068533","https://openalex.org/W2158264534","https://openalex.org/W2168912171","https://openalex.org/W2339804106","https://openalex.org/W2471674245","https://openalex.org/W2804822363"],"related_works":["https://openalex.org/W3118870734","https://openalex.org/W2025354840","https://openalex.org/W2033955364","https://openalex.org/W7095320255","https://openalex.org/W2093954839","https://openalex.org/W7096819539","https://openalex.org/W2255882457","https://openalex.org/W2612881252","https://openalex.org/W2339804106","https://openalex.org/W1969116802"],"abstract_inverted_index":{"Selenium":[0,28],"is":[1,29,78],"a":[2,24,48,73,115,139,144,201,210],"vital":[3],"micronutrient":[4],"in":[5,54,60,94,118,147,169,239,262],"many":[6],"organisms.":[7],"While":[8],"traces":[9],"are":[10,16,57],"required":[11],"for":[12,47,91,131,186,204],"survival,":[13],"excess":[14],"amounts":[15],"toxic;":[17],"thus,":[18],"selenium":[19,64,103],"can":[20],"be":[21],"regarded":[22],"as":[23,209],"biological":[25],"\"double-edged":[26],"sword\".":[27],"chemically":[30],"similar":[31],"to":[32,88],"the":[33,42,45,89,99,106,119,247,257],"essential":[34],"element":[35],"sulfur,":[36],"but":[37],"curiously,":[38],"evolution":[39],"has":[40],"selected":[41],"former":[43],"over":[44,133],"latter":[46],"subset":[49],"of":[50,62,96,102,108,195,259],"oxidoreductases.":[51],"Enzymes":[52],"involved":[53],"sulfur":[55],"metabolism":[56],"less":[58],"discriminate":[59,75],"terms":[61],"preventing":[63],"incorporation;":[65],"however,":[66],"its":[67,109,126,149],"specific":[68],"incorporation":[69],"into":[70,246],"selenoproteins":[71],"reveals":[72],"highly":[74],"process":[76],"that":[77,225],"not":[79],"completely":[80],"understood.":[81],"In":[82],"this":[83,155],"work,":[84],"we":[85,164],"add":[86],"knowledge":[87],"mechanism":[90],"selenium-over-sulfur":[92,251],"specificity":[93,130,252],"hopes":[95],"further":[97,255],"understanding":[98],"controlled":[100],"regulation":[101],"trafficking":[104],"and":[105,124,129,172,184,253],"prevention":[107],"toxicity.":[110],"We":[111],"have":[112],"identified":[113,165],"SclA,":[114,170],"selenocysteine":[116,136,260],"lyase":[117,137],"nosocomial":[120],"pathogen,":[121],"Enterococcus":[122],"faecalis,":[123],"characterized":[125,185],"enzymatic":[127],"activity":[128,196,232],"L-selenocysteine":[132],"L-cysteine.":[134],"Human":[135],"contains":[138],"residue,":[140],"D146,":[141],"which":[142],"plays":[143],"significant":[145],"role":[146,203,258],"determining":[148],"specificity.":[150],"A":[151],"D146K":[152],"mutation":[153,235],"eliminated":[154],"trait,":[156],"allowing":[157],"non-specific":[158],"L-cysteine":[159,207],"degradation.":[160],"Using":[161],"computational":[162],"biology,":[163],"an":[166],"orthologous":[167],"residue":[168],"H100,":[171],"generated":[173],"mutant":[174],"enzymes":[175,215],"with":[176,216,233],"site-directed":[177],"mutagenesis.":[178],"The":[179],"proteins":[180],"were":[181],"overexpressed,":[182],"purified,":[183],"their":[187],"biochemical":[188],"properties.":[189],"All":[190],"mutants":[191],"exhibited":[192],"varying":[193],"levels":[194],"towards":[197,213],"L-selenocysteine,":[198],"hinting":[199],"at":[200],"catalytic":[202],"H100.":[205],"Additionally,":[206],"acted":[208],"competitive":[211],"inhibitor":[212],"all":[214],"higher":[217],"affinity":[218],"than":[219],"L-selenocysteine.":[220],"Finally,":[221],"our":[222],"experiments":[223],"revealed":[224],"SclA":[226],"possessed":[227],"extremely":[228],"poor":[229],"cysteine":[230],"desulfurase":[231],"each":[234],"exhibiting":[236],"subtle":[237],"changes":[238],"turnover.":[240],"Our":[241],"findings":[242],"offer":[243],"key":[244],"insight":[245],"molecular":[248],"mechanisms":[249],"behind":[250],"may":[254],"elucidate":[256],"lyases":[261],"vivo.":[263]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2019-09-05T00:00:00"}