{"id":"https://openalex.org/W296957822","doi":null,"title":"Detection of amplified oncogenes by differential polymerase chain reaction.","display_name":"Detection of amplified oncogenes by differential polymerase chain reaction.","publication_year":1989,"publication_date":"1989-09-01","ids":{"openalex":"https://openalex.org/W296957822","mag":"296957822","pmid":"https://pubmed.ncbi.nlm.nih.gov/2780051"},"language":"en","primary_location":{"id":"pmid:2780051","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2780051","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Oncogene","raw_type":"Journal Article"},"parent_works":[],"lineage":["https://openalex.org/W296957822"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111737916","display_name":"Roy A. Frye","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R A Frye","raw_affiliation_strings":["Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27599"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lineberger Cancer Research Center, University of North Carolina, Chapel Hill 27599","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112325261","display_name":"C C Benz","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C C Benz","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5022544851","display_name":"E Liu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"E Liu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":4.9586,"has_fulltext":false,"cited_by_count":209,"citation_normalized_percentile":{"value":0.96732293,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":93},"biblio":{"volume":"4","issue":"9","first_page":"1153","last_page":"7"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9071999788284302,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9071999788284302,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10755","display_name":"HER2/EGFR in Cancer Research","score":0.0632999986410141,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11287","display_name":"Cancer Genomics and Diagnostics","score":0.01590000092983246,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/oncogene-amplification","display_name":"oncogene amplification","score":0.9850000143051147},{"id":"https://openalex.org/keywords/differential-pcr","display_name":"differential PCR","score":0.9190000295639038},{"id":"https://openalex.org/keywords/gene-copy-number","display_name":"gene copy number","score":0.8600000143051147},{"id":"https://openalex.org/keywords/epidermal-growth-factor-receptor","display_name":"epidermal growth factor receptor","score":0.9649999737739563},{"id":"https://openalex.org/keywords/breast-cancer","display_name":"breast cancer","score":0.9810000061988831},{"id":"https://openalex.org/keywords/formalin-fixed-paraffin-embedded-tissue","display_name":"formalin-fixed paraffin-embedded tissue","score":0.906000018119812},{"id":"https://openalex.org/keywords/non-radioactive-detection","display_name":"non-radioactive detection","score":0.7680000066757202}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8865053653717041},{"id":"https://openalex.org/C7602840","wikidata":"https://www.wikidata.org/wiki/Q746284","display_name":"Gene duplication","level":3,"score":0.8319873809814453},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.8182659149169922},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6498386263847351},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5656957030296326},{"id":"https://openalex.org/C2781018059","wikidata":"https://www.wikidata.org/wiki/Q207609","display_name":"Oncogene","level":4,"score":0.5377118587493896},{"id":"https://openalex.org/C28134486","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Applications of PCR","level":5,"score":0.479912132024765},{"id":"https://openalex.org/C2776657240","wikidata":"https://www.wikidata.org/wiki/Q5275341","display_name":"Differential display","level":4,"score":0.4541979134082794},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.4258206784725189},{"id":"https://openalex.org/C112456955","wikidata":"https://www.wikidata.org/wiki/Q4118048","display_name":"Hot start PCR","level":5,"score":0.42071208357810974},{"id":"https://openalex.org/C48126324","wikidata":"https://www.wikidata.org/wiki/Q5276136","display_name":"Digital polymerase chain reaction","level":4,"score":0.39973223209381104},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.35511505603790283},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.34142830967903137},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2987424433231354},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.1797732710838318},{"id":"https://openalex.org/C29537977","wikidata":"https://www.wikidata.org/wiki/Q188941","display_name":"Cell cycle","level":3,"score":0.09863901138305664}],"mesh":[{"descriptor_ui":"D001943","descriptor_name":"Breast Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D005784","descriptor_name":"Gene Amplification","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009857","descriptor_name":"Oncogenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066246","descriptor_name":"ErbB Receptors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:2780051","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2780051","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Oncogene","raw_type":"Journal Article"},{"id":"mag:296957822","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2780051","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8371999859809875,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2419761792","https://openalex.org/W2019822740","https://openalex.org/W2068828511","https://openalex.org/W2415964343","https://openalex.org/W2047764588","https://openalex.org/W2056324634","https://openalex.org/W1191570179","https://openalex.org/W2160507269","https://openalex.org/W1975990663"],"abstract_inverted_index":{"Oncogene":[0],"amplification":[1,23,79,102,108],"has":[2],"been":[3],"found":[4],"in":[5,40,69,82,121,130],"a":[6,31,57,61],"variety":[7],"of":[8,21,76,103,109,133],"human":[9],"cancers":[10],"and":[11,34,60,111],"may":[12],"have":[13,25,29],"prognostic":[14],"importance.":[15],"Therefore,":[16],"techniques":[17],"which":[18],"facilitate":[19],"detection":[20],"gene":[22,41,59,64,78,117],"could":[24,118],"wide":[26],"applicability.":[27],"We":[28,91],"devised":[30],"sensitive,":[32],"rapid,":[33],"non-radioactive":[35],"procedure":[36],"for":[37],"detecting":[38],"alterations":[39],"copy":[42],"number":[43],"based":[44],"on":[45],"the":[46,70,83,86,112],"polymerase":[47],"chain":[48],"reaction":[49,72],"(PCR).":[50],"In":[51],"this":[52,94],"technique,":[53],"called":[54],"differential":[55],"PCR,":[56],"target":[58,77,105],"single-copy":[62],"reference":[63],"are":[65],"co-amplified":[66],"by":[67],"PCR":[68],"same":[71],"vessel.":[73],"The":[74],"level":[75],"is":[80],"reflected":[81],"ratio":[84],"between":[85],"two":[87],"resulting":[88],"PCR-product":[89],"bands.":[90],"show":[92],"that":[93],"method":[95],"can":[96],"detect":[97],"as":[98,100,122,124],"low":[99],"two-fold":[101],"specific":[104],"genes.":[106],"Furthermore,":[107],"neu":[110],"epidermal":[113],"growth":[114],"factor":[115],"receptor":[116],"be":[119],"detected":[120],"few":[123],"100":[125],"breast":[126],"carcinoma":[127],"cells":[128],"or":[129],"single":[131],"sections":[132],"formalin-fixed,":[134],"embedded":[135],"material.":[136]},"counts_by_year":[{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}