{"id":"https://openalex.org/W2966565804","doi":"https://doi.org/10.1021/acs.analchem.9b01665","title":"Online Comprehensive High pH Reversed Phase \u00d7 Low pH Reversed Phase Approach for Two-Dimensional Separations of Intact Proteins in Top-Down Proteomics","display_name":"Online Comprehensive High pH Reversed Phase \u00d7 Low pH Reversed Phase Approach for Two-Dimensional Separations of Intact Proteins in Top-Down Proteomics","publication_year":2019,"publication_date":"2019-08-01","ids":{"openalex":"https://openalex.org/W2966565804","doi":"https://doi.org/10.1021/acs.analchem.9b01665","mag":"2966565804","pmid":"https://pubmed.ncbi.nlm.nih.gov/31366196"},"language":"en","primary_location":{"id":"doi:10.1021/acs.analchem.9b01665","doi":"https://doi.org/10.1021/acs.analchem.9b01665","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.analchem.9b01665","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060855729","display_name":"Yehia Z. Baghdady","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I189196454","display_name":"The University of Texas at Arlington","ror":"https://ror.org/019kgqr73","country_code":"US","type":"education","lineage":["https://openalex.org/I189196454"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yehia Z. Baghdady","raw_affiliation_strings":["Department of Chemistry & Biochemistry, The University of Texas Arlington, Arlington, Texas 76019-0065, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry & Biochemistry, The University of Texas Arlington, Arlington, Texas 76019-0065, United States","institution_ids":["https://openalex.org/I189196454"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009046602","display_name":"Kevin A. Schug","orcid":"https://orcid.org/0000-0003-4817-9508","observed_orcids":["https://orcid.org/0000-0003-4817-9508"]},"institutions":[{"id":"https://openalex.org/I189196454","display_name":"The University of Texas at Arlington","ror":"https://ror.org/019kgqr73","country_code":"US","type":"education","lineage":["https://openalex.org/I189196454"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Kevin A. Schug","raw_affiliation_strings":["Department of Chemistry & Biochemistry, The University of Texas Arlington, Arlington, Texas 76019-0065, United States"],"raw_orcid":"https://orcid.org/0000-0003-4817-9508","affiliations":[{"raw_affiliation_string":"Department of Chemistry & Biochemistry, The University of Texas Arlington, Arlington, Texas 76019-0065, United States","institution_ids":["https://openalex.org/I189196454"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5009046602"],"corresponding_institution_ids":["https://openalex.org/I189196454"],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":1.7215,"has_fulltext":false,"cited_by_count":36,"citation_normalized_percentile":{"value":0.81615966,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"91","issue":"17","first_page":"11085","last_page":"11091"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9380000233650208,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9380000233650208,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.03750000149011612,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.015699999406933784,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/top-down-proteomics","display_name":"top-down proteomics","score":0.9330000281333923},{"id":"https://openalex.org/keywords/intact-protein-separation","display_name":"intact protein separation","score":0.800000011920929},{"id":"https://openalex.org/keywords/two-dimensional-liquid-chromatography","display_name":"two-dimensional liquid chromatography","score":0.8769999742507935},{"id":"https://openalex.org/keywords/mobile-phase-ph","display_name":"mobile phase pH","score":0.6959999799728394},{"id":"https://openalex.org/keywords/isoelectric-point","display_name":"isoelectric point","score":0.656000018119812},{"id":"https://openalex.org/keywords/escherichia-coli-proteins","display_name":"Escherichia coli proteins","score":0.7870000004768372}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.9540131092071533},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.791064977645874},{"id":"https://openalex.org/C96294017","wikidata":"https://www.wikidata.org/wiki/Q308708","display_name":"Isoelectric point","level":3,"score":0.6539733409881592},{"id":"https://openalex.org/C73826308","wikidata":"https://www.wikidata.org/wiki/Q684907","display_name":"Elution","level":2,"score":0.5989136695861816},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.5862998962402344},{"id":"https://openalex.org/C154337534","wikidata":"https://www.wikidata.org/wiki/Q6005260","display_name":"Immobilized pH gradient","level":4,"score":0.5213443040847778},{"id":"https://openalex.org/C44280652","wikidata":"https://www.wikidata.org/wiki/Q104837","display_name":"Phase (matter)","level":2,"score":0.49399787187576294},{"id":"https://openalex.org/C50476208","wikidata":"https://www.wikidata.org/wiki/Q898861","display_name":"Isoelectric focusing","level":3,"score":0.48118096590042114},{"id":"https://openalex.org/C45188465","wikidata":"https://www.wikidata.org/wiki/Q645497","display_name":"Reversed-phase chromatography","level":3,"score":0.451961874961853},{"id":"https://openalex.org/C116817701","wikidata":"https://www.wikidata.org/wiki/Q5955221","display_name":"Hydrophilic interaction chromatography","level":3,"score":0.413216769695282},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.386135071516037},{"id":"https://openalex.org/C113196181","wikidata":"https://www.wikidata.org/wiki/Q485223","display_name":"Analytical Chemistry (journal)","level":2,"score":0.33986249566078186},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14267703890800476},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000075082","descriptor_name":"Proof of Concept Study","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002256","descriptor_name":"Carbonic Anhydrases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D002364","descriptor_name":"Caseins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006736","descriptor_name":"Horses","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007526","descriptor_name":"Isoelectric Point","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007768","descriptor_name":"Lactalbumin","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D007782","descriptor_name":"Lactoglobulins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D009211","descriptor_name":"Myoglobin","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D045304","descriptor_name":"Cytochromes c","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D045424","descriptor_name":"Complex Mixtures","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D056148","descriptor_name":"Chromatography, Reverse-Phase","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/acs.analchem.9b01665","doi":"https://doi.org/10.1021/acs.analchem.9b01665","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.analchem.9b01665","pdf_url":null,"source":{"id":"https://openalex.org/S191963794","display_name":"Analytical Chemistry","issn_l":"0003-2700","issn":["0003-2700","1520-6882"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical Chemistry","raw_type":"journal-article"},{"id":"pmid:31366196","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31366196","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Analytical chemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":46,"referenced_works":["https://openalex.org/W1138591158","https://openalex.org/W1965332595","https://openalex.org/W1973606653","https://openalex.org/W1981925074","https://openalex.org/W1986242002","https://openalex.org/W1988098839","https://openalex.org/W1992392890","https://openalex.org/W1995382473","https://openalex.org/W1995797614","https://openalex.org/W2003363664","https://openalex.org/W2004901341","https://openalex.org/W2016022626","https://openalex.org/W2016589213","https://openalex.org/W2034356373","https://openalex.org/W2035758756","https://openalex.org/W2043050470","https://openalex.org/W2048011760","https://openalex.org/W2052920249","https://openalex.org/W2055412026","https://openalex.org/W2056936283","https://openalex.org/W2061603007","https://openalex.org/W2064520816","https://openalex.org/W2072313644","https://openalex.org/W2090966508","https://openalex.org/W2093830786","https://openalex.org/W2095448912","https://openalex.org/W2097548092","https://openalex.org/W2138932566","https://openalex.org/W2153922223","https://openalex.org/W2229695971","https://openalex.org/W2267573512","https://openalex.org/W2295727906","https://openalex.org/W2429591244","https://openalex.org/W2462991942","https://openalex.org/W2497735451","https://openalex.org/W2515334461","https://openalex.org/W2582012543","https://openalex.org/W2618282332","https://openalex.org/W2750749432","https://openalex.org/W2761384401","https://openalex.org/W2769423498","https://openalex.org/W2777564097","https://openalex.org/W2791861326","https://openalex.org/W2796089964","https://openalex.org/W2915978142","https://openalex.org/W2933401489"],"related_works":["https://openalex.org/W2750749432","https://openalex.org/W2933401489","https://openalex.org/W2031633859","https://openalex.org/W2063859419","https://openalex.org/W2965564457","https://openalex.org/W2171874319","https://openalex.org/W3082830688","https://openalex.org/W2055412026","https://openalex.org/W4285209215","https://openalex.org/W2052365638"],"abstract_inverted_index":{"A":[0],"proof-of-concept":[1],"study":[2],"is":[3],"presented":[4],"on":[5],"the":[6,19,44,56,72,80,85,100,131,147,188,194,209],"use":[7,86],"of":[8,21,47,83,87,128,150,185,208,216],"comprehensive":[9],"two-dimensional":[10],"liquid":[11,52,68],"chromatography":[12,53,69],"mass":[13,37],"spectrometry":[14,38],"(LC":[15],"\u00d7":[16],"LC-MS)":[17],"for":[18,43,104],"separation":[20,127],"intact":[22],"protein":[23,107,217,223],"mixtures":[24],"using":[25],"a":[26,88,214],"different":[27,89],"mobile":[28,90],"phase":[29,51,67,91],"pH":[30,41,49,65,92,171,200],"in":[31,55,71,130,193,198],"each":[32],"dimension.":[33],"This":[34],"system":[35,210],"utilizes":[36],"(MS)":[39],"friendly":[40],"modifiers":[42],"online":[45],"coupling":[46],"high":[48,142,176],"reversed":[50,66],"(HPH-RPLC)":[54],"first":[57],"dimension":[58,74,133],"(":[59,75],"1":[60],"D)":[61],"followed":[62],"by":[63,120],"low":[64,170],"(LPH-RPLC)":[70],"second":[73,132],"2":[76],"D).":[77],"Owing":[78],"to":[79,95,136,165],"ionic":[81],"nature":[82],"proteins,":[84],"was":[93,134,211],"successful":[94,135],"provide":[96],"altered":[97],"selectivity":[98],"between":[99],"two":[101],"dimensions,":[102],"even":[103],"closely":[105],"related":[106],"variants,":[108],"such":[109],"as":[110],"bovine":[111],"cytochrome":[112,116],"c":[113],"and":[114,187,201,219],"equine":[115],"c,":[117],"which":[118],"differ":[119],"only":[121],"three":[122],"amino":[123],"acids.":[124],"Subminute":[125],"gradient":[126],"proteins":[129,152,161,174,186],"minimize":[137],"analysis":[138],"time,":[139],"while":[140],"maintaining":[141],"peak":[143],"capacity.":[144],"Unlike":[145],"peptides,":[146],"elution":[148,180],"order":[149],"studied":[151],"did":[153],"not":[154],"follow":[155],"their":[156],"isoelectric":[157],"points,":[158],"where":[159],"acidic":[160],"would":[162],"be":[163,166],"expected":[164],"more":[167],"retained":[168],"at":[169,175],"(and":[172],"basic":[173],"pH).":[177],"The":[178,206],"steep":[179,190],"isotherms":[181],"(on\u2013off":[182],"retention":[183],"mechanism)":[184],"very":[189],"gradients":[191],"utilized":[192],"second-dimension":[195],"column":[196],"succeeded":[197],"overcoming":[199],"organic":[202],"solvent":[203],"content":[204],"mismatch.":[205],"utility":[207],"demonstrated":[212],"with":[213],"mixture":[215],"standards":[218],"an":[220],"Escherichia":[221],"coli":[222],"mixture.":[224]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":8},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2019-08-13T00:00:00"}