{"id":"https://openalex.org/W2965516537","doi":"https://doi.org/10.17912/w2kt0n","title":"SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette","display_name":"SapTrap assembly of repair templates for Cas9-triggered homologous recombination with a self-excising cassette","publication_year":2018,"publication_date":"2018-01-01","ids":{"openalex":"https://openalex.org/W2965516537","doi":"https://doi.org/10.17912/w2kt0n","mag":"2965516537","pmid":"https://pubmed.ncbi.nlm.nih.gov/32550377","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7255845"},"language":"en","primary_location":{"id":"pmh:oai:pubmedcentral.nih.gov:7255845","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7255845","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"MicroPubl Biol","raw_type":"Text"},"volume":null,"type":"article","indexed_in":["datacite","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7255845","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009042739","display_name":"Daniel J. Dickinson","orcid":"https://orcid.org/0000-0003-2651-2584","observed_orcids":["https://orcid.org/0000-0003-2651-2584"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dickinson, Daniel J.","raw_affiliation_strings":["Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002555115","display_name":"Mark M. Slabodnick","orcid":"https://orcid.org/0000-0003-0324-1662","observed_orcids":["https://orcid.org/0000-0003-0324-1662"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Slabodnick, Mark M.","raw_affiliation_strings":["Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083232658","display_name":"Alicia H. Chen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chen, Alicia H.","raw_affiliation_strings":["Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5090874399","display_name":"Bob Goldstein","orcid":"https://orcid.org/0000-0001-6961-675X","observed_orcids":["https://orcid.org/0000-0001-6961-675X"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I4390039329","display_name":"UNC Lineberger Comprehensive Cancer Center","ror":"https://ror.org/043ehm030","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Goldstein, Bob","raw_affiliation_strings":["Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology and Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA","institution_ids":["https://openalex.org/I114027177","https://openalex.org/I4390039329"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.3341,"has_fulltext":false,"cited_by_count":31,"citation_normalized_percentile":{"value":0.8071899,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"2018","issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.8629999756813049},{"id":"https://openalex.org/keywords/homologous-recombination","display_name":"homologous recombination","score":0.8339999914169312},{"id":"https://openalex.org/keywords/caenorhabditis-elegans","display_name":"Caenorhabditis elegans","score":0.8870000243186951},{"id":"https://openalex.org/keywords/drug-selection","display_name":"drug selection","score":0.6769999861717224},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.6600000262260437}],"concepts":[{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.6591821908950806},{"id":"https://openalex.org/C82714645","wikidata":"https://www.wikidata.org/wiki/Q438331","display_name":"Template","level":2,"score":0.6426326632499695},{"id":"https://openalex.org/C64894306","wikidata":"https://www.wikidata.org/wiki/Q849622","display_name":"Homologous chromosome","level":3,"score":0.5415281653404236},{"id":"https://openalex.org/C156695909","wikidata":"https://www.wikidata.org/wiki/Q3373825","display_name":"Recombination","level":3,"score":0.43355119228363037},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.36876702308654785},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3141091465950012},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2965488135814667},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.1482553780078888},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.06949526071548462},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.060123831033706665}],"mesh":[],"locations_count":4,"locations":[{"id":"pmh:oai:pubmedcentral.nih.gov:7255845","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7255845","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"MicroPubl Biol","raw_type":"Text"},{"id":"pmid:32550377","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32550377","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"microPublication biology","raw_type":"Journal Article"},{"id":"doi:10.17912/w2kt0n","doi":"https://doi.org/10.17912/w2kt0n","is_oa":true,"landing_page_url":"https://doi.org/10.17912/w2kt0n","pdf_url":null,"source":{"id":"https://openalex.org/S7407050849","display_name":"microPublication","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I122411786","host_organization_name":"California Institute of Technology","host_organization_lineage":["https://openalex.org/I122411786"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"DataPaper"},{"id":"mag:2965516537","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32550377","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:7255845","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7255845","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"MicroPubl Biol","raw_type":"Text"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1211544634","display_name":"C. elegans Gastrulation: a Model for Understanding Apical Constriction Mechanisms","funder_award_id":"R01GM083071","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G5680636581","display_name":"Biochemical Basis of Cell Polarization by the PAR Protein System","funder_award_id":"K99GM115964","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":5,"referenced_works":["https://openalex.org/W2110819817","https://openalex.org/W2132654115","https://openalex.org/W2287904753","https://openalex.org/W2296811223","https://openalex.org/W2471526055"],"related_works":["https://openalex.org/W2287904753","https://openalex.org/W3190808470","https://openalex.org/W2963399338","https://openalex.org/W7097765545","https://openalex.org/W2060297895","https://openalex.org/W2236005851","https://openalex.org/W2132654115","https://openalex.org/W2976082486","https://openalex.org/W3200140367","https://openalex.org/W7097481094"],"abstract_inverted_index":{"The":[0,70,109],"advent":[1],"of":[2,13,27,80,102,169,172,192],"CRISPR/Cas9":[3],"technology":[4],"in":[5,23,36,74,143],"C.":[6],"elegans":[7],"has":[8],"enabled":[9],"an":[10,149],"unprecedented":[11],"level":[12],"control":[14],"over":[15,175],"this":[16,37,75],"organism\u2019s":[17],"genome,":[18],"which":[19],"is":[20,77,93,137],"facilitating":[21],"research":[22],"a":[24,48,52,81,94,123,144,190],"wide":[25],"variety":[26],"fields.":[28],"Several":[29],"different":[30],"experimental":[31],"approaches":[32],"exist":[33],"for":[34,85,105,127],"CRISPR":[35],"organism":[38],"(Dickinson":[39,66,164],"and":[40,90,117,147],"Goldstein":[41],"2016).":[42],"One":[43],"particularly":[44],"powerful":[45],"approach":[46],"uses":[47],"drug":[49],"selection":[50,125,136,159,174],"with":[51],"Self-Excising":[53],"Cassette":[54],"(SEC)":[55],"to":[56,132,156,179],"isolate":[57],"the":[58,78,158,181,184],"desired":[59],"genetically-modified":[60],"animals":[61],"following":[62],"Cas9-triggered":[63],"homologous":[64,86,107],"recombination":[65],"et":[67,165],"al.":[68,166],"2015).":[69,167],"most":[71],"labor-intensive":[72],"step":[73,152],"procedure":[76,97],"construction":[79],"plasmid-based":[82],"repair":[83,103,198],"template":[84],"recombination.":[87,108],"SapTrap":[88,114,185,201],"(Schwartz":[89,116],"Jorgensen":[91,118],"2016)":[92,119],"high-throughput":[95],"cloning":[96,115],"that":[98,112,194],"allows":[99],"modular":[100],"assembly":[101],"templates":[104,199],"CRISPR/Cas9-triggered":[106],"original":[110],"publication":[111],"described":[113],"used":[120,141],"unc-119(+)":[121],"as":[122],"positive":[124],"marker":[126],"isolating":[128],"recombinant":[129],"strains.":[130],"Compared":[131],"unc-119":[133],"selection,":[134],"SEC":[135,173,182],"faster;":[138],"can":[139],"be":[140],"directly":[142],"wild-type":[145],"background;":[146],"eliminates":[148],"extra":[150],"injection":[151],"followed":[153],"by":[154],"outcrossing":[155],"remove":[157],"markers":[160],"after":[161],"strain":[162],"isolation":[163],"Because":[168],"these":[170],"advantages":[171],"unc-119,":[176],"we":[177,188],"wished":[178],"incorporate":[180],"into":[183],"workflow.":[186],"Here,":[187],"describe":[189],"toolkit":[191],"plasmids":[193],"allow":[195],"building":[196],"SEC-containing":[197],"via":[200],"cloning.":[202]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":1}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2019-08-13T00:00:00"}
