{"id":"https://openalex.org/W2963370933","doi":"https://doi.org/10.3389/fcimb.2019.00287","title":"Visualization of Spirochetes by Labeling Membrane Proteins With Fluorescent Biarsenical Dyes","display_name":"Visualization of Spirochetes by Labeling Membrane Proteins With Fluorescent Biarsenical Dyes","publication_year":2019,"publication_date":"2019-08-20","ids":{"openalex":"https://openalex.org/W2963370933","doi":"https://doi.org/10.3389/fcimb.2019.00287","mag":"2963370933","pmid":"https://pubmed.ncbi.nlm.nih.gov/31482073","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6710359"},"language":"en","primary_location":{"id":"doi:10.3389/fcimb.2019.00287","doi":"https://doi.org/10.3389/fcimb.2019.00287","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fcimb.2019.00287","pdf_url":"https://www.frontiersin.org/articles/10.3389/fcimb.2019.00287/pdf","source":{"id":"https://openalex.org/S2506667490","display_name":"Frontiers in Cellular and Infection Microbiology","issn_l":"2235-2988","issn":["2235-2988"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Cellular and Infection Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/articles/10.3389/fcimb.2019.00287/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5041256425","display_name":"Chadwick Hillman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"id":"https://openalex.org/I4405287673","display_name":"Rocky Mountain Laboratories","ror":"https://ror.org/05v7g7w67","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Chadwick Hillman","raw_affiliation_strings":["Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065461587","display_name":"Philip E. Stewart","orcid":"https://orcid.org/0000-0002-7217-0303","observed_orcids":["https://orcid.org/0000-0002-7217-0303"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"id":"https://openalex.org/I4405287673","display_name":"Rocky Mountain Laboratories","ror":"https://ror.org/05v7g7w67","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Philip E. Stewart","raw_affiliation_strings":["Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022726916","display_name":"Martin Strnad","orcid":"https://orcid.org/0000-0002-1671-6252","observed_orcids":["https://orcid.org/0000-0002-1671-6252","https://orcid.org/0009-0000-1896-9889"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I3124161356","display_name":"University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice","ror":"https://ror.org/033n3pw66","country_code":"CZ","type":"education","lineage":["https://openalex.org/I3124161356"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"id":"https://openalex.org/I4210161358","display_name":"Institute of Parasitology","ror":"https://ror.org/05rhyza23","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I202391551","https://openalex.org/I4210161358","https://openalex.org/I4210164740"]},{"id":"https://openalex.org/I4210164740","display_name":"Czech Academy of Sciences, Biology Centre","ror":"https://ror.org/05pq4yn02","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I202391551","https://openalex.org/I4210164740"]},{"id":"https://openalex.org/I4405287673","display_name":"Rocky Mountain Laboratories","ror":"https://ror.org/05v7g7w67","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}],"countries":["CZ","US"],"is_corresponding":false,"raw_author_name":"Martin Strnad","raw_affiliation_strings":["Faculty of Science, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, \u010cesk\u00e9 Bud\u011bjovice, Czechia","Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, \u010cesk\u00e9 Bud\u011bjovice, Czechia","Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Science, University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice, \u010cesk\u00e9 Bud\u011bjovice, Czechia","institution_ids":["https://openalex.org/I3124161356"]},{"raw_affiliation_string":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, \u010cesk\u00e9 Bud\u011bjovice, Czechia","institution_ids":["https://openalex.org/I4210161358","https://openalex.org/I4210164740"]},{"raw_affiliation_string":"Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075606855","display_name":"Hunter H. Stone","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"id":"https://openalex.org/I4405287673","display_name":"Rocky Mountain Laboratories","ror":"https://ror.org/05v7g7w67","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hunter Stone","raw_affiliation_strings":["Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076459412","display_name":"Tregei Starr","orcid":"https://orcid.org/0000-0003-1561-8834","observed_orcids":["https://orcid.org/0000-0003-1561-8834"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"id":"https://openalex.org/I4405287673","display_name":"Rocky Mountain Laboratories","ror":"https://ror.org/05v7g7w67","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tregei Starr","raw_affiliation_strings":["Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079337385","display_name":"Aaron Carmody","orcid":"https://orcid.org/0000-0003-4772-763X","observed_orcids":["https://orcid.org/0000-0003-4772-763X"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"id":"https://openalex.org/I4405287673","display_name":"Rocky Mountain Laboratories","ror":"https://ror.org/05v7g7w67","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aaron Carmody","raw_affiliation_strings":["Research Technologies Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Technologies Section, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112456691","display_name":"Tyler J. Evans","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"id":"https://openalex.org/I4405287673","display_name":"Rocky Mountain Laboratories","ror":"https://ror.org/05v7g7w67","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tyler J. Evans","raw_affiliation_strings":["Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044484247","display_name":"Valentina Carracoi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"id":"https://openalex.org/I4405287673","display_name":"Rocky Mountain Laboratories","ror":"https://ror.org/05v7g7w67","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Valentina Carracoi","raw_affiliation_strings":["Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003920067","display_name":"Jenny Wachter","orcid":"https://orcid.org/0000-0003-2175-5595","observed_orcids":["https://orcid.org/0000-0003-2175-5595"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"id":"https://openalex.org/I4405287673","display_name":"Rocky Mountain Laboratories","ror":"https://ror.org/05v7g7w67","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jenny Wachter","raw_affiliation_strings":["Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073105910","display_name":"Patricia A. Rosa","orcid":"https://orcid.org/0000-0002-9489-3406","observed_orcids":["https://orcid.org/0000-0002-9489-3406"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210134534","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534"]},{"id":"https://openalex.org/I4405287673","display_name":"Rocky Mountain Laboratories","ror":"https://ror.org/05v7g7w67","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Patricia A. Rosa","raw_affiliation_strings":["Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5073105910"],"corresponding_institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210134534","https://openalex.org/I4405287673"],"apc_list":{"value":2950,"currency":"USD","value_usd":2950},"apc_paid":{"value":2950,"currency":"USD","value_usd":2950},"fwci":0.7971,"has_fulltext":true,"cited_by_count":12,"citation_normalized_percentile":{"value":0.66672714,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"9","issue":null,"first_page":"287","last_page":"287"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.5698000192642212,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.5698000192642212,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.17350000143051147,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.05429999902844429,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/spirochetes","display_name":"spirochetes","score":0.9409999847412109},{"id":"https://openalex.org/keywords/flash","display_name":"Adobe Flash","score":0.8939999938011169},{"id":"https://openalex.org/keywords/tetracysteine-motif","display_name":"tetracysteine motif","score":0.9879999756813049},{"id":"https://openalex.org/keywords/membrane-protein-labeling","display_name":"membrane protein labeling","score":0.8100000023841858},{"id":"https://openalex.org/keywords/live-cell-imaging","display_name":"live-cell imaging","score":0.902999997138977},{"id":"https://openalex.org/keywords/leptospira","display_name":"Leptospira","score":0.9610000252723694},{"id":"https://openalex.org/keywords/borrelia-burgdorferi","display_name":"Borrelia burgdorferi","score":0.9950000047683716},{"id":"https://openalex.org/keywords/outer-membrane-proteins","display_name":"outer membrane proteins","score":0.7099999785423279},{"id":"https://openalex.org/keywords/spirochete-motility","display_name":"spirochete motility","score":0.8500000238418579},{"id":"https://openalex.org/keywords/spirochete-morphology","display_name":"spirochete morphology","score":0.9610000252723694}],"concepts":[{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.663038969039917},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5858160853385925},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.5504610538482666},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5284250378608704},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4972279369831085},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.48350098729133606},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.46710124611854553},{"id":"https://openalex.org/C2779178360","wikidata":"https://www.wikidata.org/wiki/Q902045","display_name":"Confocal microscopy","level":2,"score":0.43597492575645447},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4272412657737732},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.38228175044059753},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.35736316442489624},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.339248925447464},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09203529357910156},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013144","descriptor_name":"Spirochaetales","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D013144","descriptor_name":"Spirochaetales","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D013144","descriptor_name":"Spirochaetales","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013145","descriptor_name":"Spirochaetales Infections","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D013194","descriptor_name":"Staining and Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.3389/fcimb.2019.00287","doi":"https://doi.org/10.3389/fcimb.2019.00287","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fcimb.2019.00287","pdf_url":"https://www.frontiersin.org/articles/10.3389/fcimb.2019.00287/pdf","source":{"id":"https://openalex.org/S2506667490","display_name":"Frontiers in Cellular and Infection Microbiology","issn_l":"2235-2988","issn":["2235-2988"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Cellular and Infection Microbiology","raw_type":"journal-article"},{"id":"pmid:31482073","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31482073","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in cellular and infection microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6710359","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC6710359/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Front Cell Infect Microbiol","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:fed724da265449c4bdce84b0dcad084d","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/fed724da265449c4bdce84b0dcad084d","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Cellular and Infection Microbiology, Vol 9 (2019)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3389/fcimb.2019.00287","doi":"https://doi.org/10.3389/fcimb.2019.00287","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fcimb.2019.00287","pdf_url":"https://www.frontiersin.org/articles/10.3389/fcimb.2019.00287/pdf","source":{"id":"https://openalex.org/S2506667490","display_name":"Frontiers in Cellular and Infection Microbiology","issn_l":"2235-2988","issn":["2235-2988"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Cellular and Infection Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G307179124","display_name":"Electron microscopy","funder_award_id":"TE01020118","funder_id":"https://openalex.org/F4320315323","funder_display_name":"Technology Agency of the Czech Republic"}],"funders":[{"id":"https://openalex.org/F4320309123","display_name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"},{"id":"https://openalex.org/F4320315323","display_name":"Technology Agency of the Czech Republic","ror":null},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2963370933.pdf","grobid_xml":"https://content.openalex.org/works/W2963370933.grobid-xml"},"referenced_works_count":51,"referenced_works":["https://openalex.org/W1589018765","https://openalex.org/W1836845300","https://openalex.org/W1860685051","https://openalex.org/W1879772459","https://openalex.org/W1963585853","https://openalex.org/W1968709514","https://openalex.org/W1973562367","https://openalex.org/W1984445119","https://openalex.org/W1990064965","https://openalex.org/W1991262857","https://openalex.org/W2005866277","https://openalex.org/W2009333227","https://openalex.org/W2010712022","https://openalex.org/W2014268903","https://openalex.org/W2015096711","https://openalex.org/W2022368410","https://openalex.org/W2022719897","https://openalex.org/W2035841833","https://openalex.org/W2036154785","https://openalex.org/W2040595568","https://openalex.org/W2063367477","https://openalex.org/W2075867327","https://openalex.org/W2083750561","https://openalex.org/W2104354772","https://openalex.org/W2109039744","https://openalex.org/W2110367525","https://openalex.org/W2111485154","https://openalex.org/W2111720407","https://openalex.org/W2118583609","https://openalex.org/W2131492700","https://openalex.org/W2139312520","https://openalex.org/W2142551946","https://openalex.org/W2149695505","https://openalex.org/W2150491501","https://openalex.org/W2163822517","https://openalex.org/W2164512091","https://openalex.org/W2169525254","https://openalex.org/W2169775775","https://openalex.org/W2169993663","https://openalex.org/W2170069130","https://openalex.org/W2220103277","https://openalex.org/W2271708860","https://openalex.org/W2290524349","https://openalex.org/W2434038410","https://openalex.org/W2483584999","https://openalex.org/W2563065268","https://openalex.org/W2598743245","https://openalex.org/W2747435985","https://openalex.org/W2801096251","https://openalex.org/W2901723220","https://openalex.org/W2902519001"],"related_works":["https://openalex.org/W2069695895","https://openalex.org/W7099203369","https://openalex.org/W2076353694","https://openalex.org/W2035460066","https://openalex.org/W2032832029","https://openalex.org/W1984941327","https://openalex.org/W1973562367","https://openalex.org/W119785679","https://openalex.org/W3195631839","https://openalex.org/W2146199522"],"abstract_inverted_index":{"Numerous":[0],"methods":[1],"exist":[2],"for":[3,93,207],"fluorescently":[4],"labeling":[5,55,105],"proteins":[6,11,26,41,56,110,199],"either":[7],"as":[8],"direct":[9],"fusion":[10],"(GFP,":[12],"RFP,":[13],"YFP,":[14],"etc.":[15],"-":[16],"attached":[17],"to":[18,27],"the":[19,33,75,87,144,155,161,169,183,189,193,205],"protein":[20,48,180],"of":[21,81,90,96,99,116,143,154,192,198,216],"interest)":[22],"or":[23,45,52,107,209],"utilizing":[24],"accessory":[25],"produce":[28],"fluorescence":[29,122],"(SNAP-tag,":[30],"CLIP-tag),":[31],"but":[32],"significant":[34],"increase":[35,129],"in":[36,130,159,200],"size":[37],"that":[38,73,160],"these":[39],"accompanying":[40],"add":[42],"may":[43],"hinder":[44],"impede":[46],"proper":[47],"folding,":[49],"cellular":[50,202],"localization,":[51],"oligomerization.":[53],"Fluorescently":[54],"with":[57,112,179,225],"biarsenical":[58,156],"dyes,":[59],"like":[60],"FlAsH,":[61],"circumvents":[62],"this":[63,212],"issue":[64],"by":[65,104,121,139],"using":[66],"a":[67],"short":[68],"6-amino":[69,145],"acid":[70,146],"tetracysteine":[71,113,170],"motif":[72,171],"binds":[74],"membrane-permeable":[76,190],"dye":[77,92,157],"and":[78,102,124,175,188,221],"allows":[79],"visualization":[80],"living":[82],"cells.":[83],"Here,":[84],"we":[85,149],"report":[86],"successful":[88],"adaptation":[89],"FlAsH":[91],"live-cell":[94],"imaging":[95],"two":[97,141],"genera":[98],"spirochetes,":[100],"Leptospira":[101],"Borrelia,":[103],"inner":[106],"outer":[108],"membrane":[109],"tagged":[111],"motifs.":[114],"Visualization":[115],"labeled":[117],"spirochetes":[118],"was":[119,137],"possible":[120],"microscopy":[123],"flow":[125],"cytometry.":[126],"A":[127],"subsequent":[128],"fluorescent":[131,196,227],"signal":[132],"intensity,":[133],"including":[134],"prolonged":[135],"detection,":[136],"achieved":[138],"concatenating":[140],"copies":[142],"motif.":[147],"Overall,":[148],"demonstrate":[150],"several":[151],"positive":[152],"attributes":[153],"system":[158],"technique":[162],"is":[163,172],"broadly":[164],"applicable":[165],"across":[166],"spirochete":[167,219],"genera,":[168],"stably":[173],"retained":[174],"does":[176],"not":[177],"interfere":[178],"function":[181],"throughout":[182],"B.":[184],"burgdorferi":[185],"infectious":[186],"cycle,":[187],"nature":[191],"dyes":[194],"permits":[195],"detection":[197],"different":[201],"locations":[203],"without":[204],"need":[206],"fixation":[208],"permeabilization.":[210],"Using":[211],"method,":[213],"new":[214],"avenues":[215],"investigation":[217],"into":[218],"morphology":[220],"motility,":[222],"previously":[223],"inaccessible":[224],"large":[226],"proteins,":[228],"can":[229],"now":[230],"be":[231],"explored.":[232]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2020,"cited_by_count":5}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2019-07-30T00:00:00"}