{"id":"https://openalex.org/W2954718153","doi":"https://doi.org/10.2144/btn-2019-0044","title":"High-Throughput Quantitative Detection of Basal Autophagy and Autophagic Flux Using Image Cytometry","display_name":"High-Throughput Quantitative Detection of Basal Autophagy and Autophagic Flux Using Image Cytometry","publication_year":2019,"publication_date":"2019-06-26","ids":{"openalex":"https://openalex.org/W2954718153","doi":"https://doi.org/10.2144/btn-2019-0044","mag":"2954718153","pmid":"https://pubmed.ncbi.nlm.nih.gov/31238709","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7141596"},"language":"en","primary_location":{"id":"doi:10.2144/btn-2019-0044","doi":"https://doi.org/10.2144/btn-2019-0044","is_oa":true,"landing_page_url":"https://doi.org/10.2144/btn-2019-0044","pdf_url":"https://www.future-science.com/doi/pdf/10.2144/btn-2019-0044","source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.future-science.com/doi/pdf/10.2144/btn-2019-0044","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028980866","display_name":"Gopika SenthilKumar","orcid":"https://orcid.org/0000-0001-5781-1056","observed_orcids":["https://orcid.org/0000-0001-5781-1056"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]},{"id":"https://openalex.org/I4210103341","display_name":"University of Wisconsin Carbone Cancer Center","ror":"https://ror.org/01e4byj08","country_code":"US","type":"funder","lineage":["https://openalex.org/I135310074","https://openalex.org/I2799905417","https://openalex.org/I4210103341","https://openalex.org/I4210132596"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gopika SenthilKumar","raw_affiliation_strings":["Department of Human Oncology and UW Carbone Cancer Center \u2013 University of Wisconsin School of Medicine and Public Health","Department of Human Oncology & UW Carbone Cancer Center, University of Wisconsin School of Medicine & Public Health, 750 Highland Ave, Madison, WI53726, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Human Oncology and UW Carbone Cancer Center \u2013 University of Wisconsin School of Medicine and Public Health","institution_ids":["https://openalex.org/I135310074","https://openalex.org/I4210103341"]},{"raw_affiliation_string":"Department of Human Oncology & UW Carbone Cancer Center, University of Wisconsin School of Medicine & Public Health, 750 Highland Ave, Madison, WI53726, USA","institution_ids":["https://openalex.org/I135310074","https://openalex.org/I4210103341"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052995393","display_name":"Justin H. Skiba","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]},{"id":"https://openalex.org/I4210103341","display_name":"University of Wisconsin Carbone Cancer Center","ror":"https://ror.org/01e4byj08","country_code":"US","type":"funder","lineage":["https://openalex.org/I135310074","https://openalex.org/I2799905417","https://openalex.org/I4210103341","https://openalex.org/I4210132596"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Justin H. Skiba","raw_affiliation_strings":["Department of Human Oncology and UW Carbone Cancer Center \u2013 University of Wisconsin School of Medicine and Public Health","Department of Human Oncology & UW Carbone Cancer Center, University of Wisconsin School of Medicine & Public Health, 750 Highland Ave, Madison, WI53726, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Human Oncology and UW Carbone Cancer Center \u2013 University of Wisconsin School of Medicine and Public Health","institution_ids":["https://openalex.org/I135310074","https://openalex.org/I4210103341"]},{"raw_affiliation_string":"Department of Human Oncology & UW Carbone Cancer Center, University of Wisconsin School of Medicine & Public Health, 750 Highland Ave, Madison, WI53726, USA","institution_ids":["https://openalex.org/I135310074","https://openalex.org/I4210103341"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073175528","display_name":"Randall J. Kimple","orcid":"https://orcid.org/0000-0001-8336-8000","observed_orcids":["https://orcid.org/0000-0001-8336-8000"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]},{"id":"https://openalex.org/I4210103341","display_name":"University of Wisconsin Carbone Cancer Center","ror":"https://ror.org/01e4byj08","country_code":"US","type":"funder","lineage":["https://openalex.org/I135310074","https://openalex.org/I2799905417","https://openalex.org/I4210103341","https://openalex.org/I4210132596"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Randall J. Kimple","raw_affiliation_strings":["Department of Human Oncology and UW Carbone Cancer Center \u2013 University of Wisconsin School of Medicine and Public Health","Department of Human Oncology & UW Carbone Cancer Center, University of Wisconsin School of Medicine & Public Health, 750 Highland Ave, Madison, WI 53726, USA ;","Department of Human Oncology & UW Carbone Cancer Center, University of Wisconsin School of Medicine & Public Health, 750 Highland Ave, Madison, WI53726, USA"],"raw_orcid":"https://orcid.org/0000-0001-8336-8000","affiliations":[{"raw_affiliation_string":"Department of Human Oncology and UW Carbone Cancer Center \u2013 University of Wisconsin School of Medicine and Public Health","institution_ids":["https://openalex.org/I135310074","https://openalex.org/I4210103341"]},{"raw_affiliation_string":"Department of Human Oncology & UW Carbone Cancer Center, University of Wisconsin School of Medicine & Public Health, 750 Highland Ave, Madison, WI 53726, USA ;","institution_ids":["https://openalex.org/I135310074","https://openalex.org/I4210103341"]},{"raw_affiliation_string":"Department of Human Oncology & UW Carbone Cancer Center, University of Wisconsin School of Medicine & Public Health, 750 Highland Ave, Madison, WI53726, USA","institution_ids":["https://openalex.org/I135310074","https://openalex.org/I4210103341"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5073175528"],"corresponding_institution_ids":["https://openalex.org/I135310074","https://openalex.org/I4210103341"],"apc_list":{"value":1415,"currency":"USD","value_usd":1415},"apc_paid":{"value":1415,"currency":"USD","value_usd":1415},"fwci":0.7404,"has_fulltext":true,"cited_by_count":18,"citation_normalized_percentile":{"value":0.61200476,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"67","issue":"2","first_page":"70","last_page":"73"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/basal-autophagy","display_name":"basal autophagy","score":0.8700000047683716},{"id":"https://openalex.org/keywords/autophagic-flux","display_name":"autophagic flux","score":0.9980000257492065},{"id":"https://openalex.org/keywords/image-cytometry","display_name":"image cytometry","score":0.8980000019073486},{"id":"https://openalex.org/keywords/acridine-orange-staining","display_name":"acridine orange staining","score":0.9929999709129333},{"id":"https://openalex.org/keywords/high-throughput-quantification","display_name":"high-throughput quantification","score":0.8700000047683716}],"concepts":[{"id":"https://openalex.org/C2779102032","wikidata":"https://www.wikidata.org/wiki/Q342717","display_name":"Acridine orange","level":3,"score":0.8202364444732666},{"id":"https://openalex.org/C203522944","wikidata":"https://www.wikidata.org/wiki/Q288322","display_name":"Autophagy","level":3,"score":0.7547270059585571},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.7016701102256775},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5479301810264587},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4665873646736145},{"id":"https://openalex.org/C2780339063","wikidata":"https://www.wikidata.org/wiki/Q5201367","display_name":"Cytometry","level":3,"score":0.4537442624568939},{"id":"https://openalex.org/C2778236600","wikidata":"https://www.wikidata.org/wiki/Q592324","display_name":"Immunofluorescence","level":3,"score":0.4483003318309784},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.409172385931015},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.25903263688087463},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.14696946740150452},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13149234652519226},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.12699934840202332},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.09548068046569824},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07053226232528687}],"mesh":[{"descriptor_ui":"D000165","descriptor_name":"Acridine Orange","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D001343","descriptor_name":"Autophagy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019044","descriptor_name":"Image Cytometry","qualifier_ui":"Q000191","qualifier_name":"economics","is_major_topic":false},{"descriptor_ui":"D019044","descriptor_name":"Image Cytometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.2144/btn-2019-0044","doi":"https://doi.org/10.2144/btn-2019-0044","is_oa":true,"landing_page_url":"https://doi.org/10.2144/btn-2019-0044","pdf_url":"https://www.future-science.com/doi/pdf/10.2144/btn-2019-0044","source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},{"id":"doi:10.1101/590513","doi":"https://doi.org/10.1101/590513","is_oa":true,"landing_page_url":"https://doi.org/10.1101/590513","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2019/06/01/590513.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:31238709","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31238709","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7141596","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7141596","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Biotechniques","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:1481e496358b482a86a0d92e0b3d27e7","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/1481e496358b482a86a0d92e0b3d27e7","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BioTechniques, Vol 67, Iss 2, Pp 70-73 (2019)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.2144/btn-2019-0044","doi":"https://doi.org/10.2144/btn-2019-0044","is_oa":true,"landing_page_url":"https://doi.org/10.2144/btn-2019-0044","pdf_url":"https://www.future-science.com/doi/pdf/10.2144/btn-2019-0044","source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1712026730","display_name":null,"funder_award_id":"CA014520","funder_id":"https://openalex.org/F4320310176","funder_display_name":"University of Wisconsin Carbone Cancer Center"},{"id":"https://openalex.org/G3398397080","display_name":null,"funder_award_id":"P50 DE026787","funder_id":"https://openalex.org/F4320310176","funder_display_name":"University of Wisconsin Carbone Cancer Center"},{"id":"https://openalex.org/G4004214011","display_name":null,"funder_award_id":"P30 CA 014520","funder_id":"https://openalex.org/F4320310176","funder_display_name":"University of Wisconsin Carbone Cancer Center"},{"id":"https://openalex.org/G4409443574","display_name":"Head and Neck Cancer SPORE at the University of Wisconsin","funder_award_id":"P50DE026787","funder_id":"https://openalex.org/F4320337365","funder_display_name":"National Institute of Dental and Craniofacial Research"},{"id":"https://openalex.org/G6230785764","display_name":null,"funder_award_id":"P30 CA014520","funder_id":"https://openalex.org/F4320306095","funder_display_name":"American Cancer Society"},{"id":"https://openalex.org/G6781161026","display_name":null,"funder_award_id":"NIH P50","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000870050","display_name":"UW Comprehensive Cancer Center Support","funder_award_id":"P30CA014520","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320306095","display_name":"American Cancer Society","ror":"https://ror.org/02e463172"},{"id":"https://openalex.org/F4320310176","display_name":"University of Wisconsin Carbone Cancer Center","ror":"https://ror.org/02mqqhj42"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337365","display_name":"National Institute of Dental and Craniofacial Research","ror":"https://ror.org/004a2wv92"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2954718153.pdf","grobid_xml":"https://content.openalex.org/works/W2954718153.grobid-xml"},"referenced_works_count":13,"referenced_works":["https://openalex.org/W200408074","https://openalex.org/W1972517547","https://openalex.org/W1990138203","https://openalex.org/W1998319919","https://openalex.org/W2004325124","https://openalex.org/W2025665129","https://openalex.org/W2034117525","https://openalex.org/W2044621729","https://openalex.org/W2079408380","https://openalex.org/W2167279371","https://openalex.org/W2551865970","https://openalex.org/W2566116490","https://openalex.org/W2748137738"],"related_works":["https://openalex.org/W4313379122","https://openalex.org/W1904548502","https://openalex.org/W2483753916","https://openalex.org/W2062028090","https://openalex.org/W2416527506","https://openalex.org/W1488407312","https://openalex.org/W2318906529","https://openalex.org/W4379405294","https://openalex.org/W1549104816","https://openalex.org/W2737322508"],"abstract_inverted_index":{"Abstract":[0],"Quantitative":[1],"assessment":[2],"of":[3,13,16,127],"changes":[4,41,91],"in":[5,19,42,92],"macro-autophagy":[6],"is":[7,25,36,119],"often":[8],"performed":[9],"through":[10],"manual":[11],"quantification":[12],"the":[14],"number":[15],"LC3B":[17],"foci":[18],"immunofluorescence":[20],"microscopy":[21,54],"images.":[22],"This":[23,121],"method":[24,84,110],"highly":[26,56],"laborious,":[27],"subject":[28],"to":[29,67,133],"image-field":[30],"selection":[31],"and":[32,35,51,60,70,82,89,98,108,116,130],"foci-counting":[33],"bias,":[34],"not":[37],"sensitive":[38],"for":[39,85,111],"analyzing":[40],"basal":[43,87,114],"autophagy.":[44,135],"Alternative":[45],"methods":[46],"such":[47],"as":[48],"flow":[49],"cytometry":[50,97,132],"transmission":[52],"electron":[53],"require":[55],"specialized,":[57],"expensive":[58],"instruments":[59],"time-consuming":[61],"sample":[62],"preparation.":[63],"Immunoblots":[64],"are":[65],"prone":[66],"exposure-related":[68],"variations":[69],"noise":[71],"that":[72],"prevent":[73],"accurate":[74],"quantification.":[75],"We":[76],"report":[77],"a":[78],"high-throughput,":[79,105],"inexpensive,":[80,106],"reliable,":[81,107],"objective":[83,109],"studying":[86,112],"level":[88],"flux":[90,118],"late-stage":[93,128],"autophagy":[94,115,129],"using":[95],"image":[96,131],"acridine":[99,124],"orange":[100,125],"staining.":[101],"Methods":[102],"summary":[103],"A":[104],"both":[113],"autophagic":[117],"reported.":[120],"approach":[122],"uses":[123],"staining":[126],"quantify":[134]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2019-07-12T00:00:00"}