{"id":"https://openalex.org/W2952298077","doi":"https://doi.org/10.1186/s12864-019-5480-0","title":"Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding","display_name":"Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding","publication_year":2019,"publication_date":"2019-02-06","ids":{"openalex":"https://openalex.org/W2952298077","doi":"https://doi.org/10.1186/s12864-019-5480-0","mag":"2952298077","pmid":"https://pubmed.ncbi.nlm.nih.gov/30727954","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6364396"},"language":"en","primary_location":{"id":"doi:10.1186/s12864-019-5480-0","doi":"https://doi.org/10.1186/s12864-019-5480-0","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12864-019-5480-0","pdf_url":"https://bmcgenomics.biomedcentral.com/counter/pdf/10.1186/s12864-019-5480-0","source":{"id":"https://openalex.org/S28614077","display_name":"BMC Genomics","issn_l":"1471-2164","issn":["1471-2164"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Genomics","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://bmcgenomics.biomedcentral.com/counter/pdf/10.1186/s12864-019-5480-0","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5029765088","display_name":"Michael Boettcher","orcid":"https://orcid.org/0000-0003-0986-4465","observed_orcids":["https://orcid.org/0000-0003-0986-4465"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael Boettcher","raw_affiliation_strings":["Department of Microbiology and Immunology, UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074605802","display_name":"Sergio Covarrubias","orcid":"https://orcid.org/0000-0002-7197-9952","observed_orcids":["https://orcid.org/0000-0002-7197-9952"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sergio Covarrubias","raw_affiliation_strings":["Department of Microbiology and Immunology, UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053239891","display_name":"Anne Biton","orcid":"https://orcid.org/0000-0002-3814-4195","observed_orcids":["https://orcid.org/0000-0002-3814-4195"]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I157536573","display_name":"Institut Pasteur","ror":"https://ror.org/0495fxg12","country_code":"FR","type":"nonprofit","lineage":["https://openalex.org/I157536573"]},{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["FR","US"],"is_corresponding":false,"raw_author_name":"Anne Biton","raw_affiliation_strings":["Centre de Bioinformatique, Biostatistique et Biologie Int\u00e9grative (C3BI, USR 3756 Institut Pasteur et CNRS), Paris, France","Department of Medicine, Lung Biology Center, University of California, San Francisco, San Francisco, California, USA","Department of Medicine, Lung Biology Center, University of California, San Francisco, San Francisco, 94143, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Centre de Bioinformatique, Biostatistique et Biologie Int\u00e9grative (C3BI, USR 3756 Institut Pasteur et CNRS), Paris, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I157536573"]},{"raw_affiliation_string":"Department of Medicine, Lung Biology Center, University of California, San Francisco, San Francisco, California, USA","institution_ids":["https://openalex.org/I180670191"]},{"raw_affiliation_string":"Department of Medicine, Lung Biology Center, University of California, San Francisco, San Francisco, 94143, CA, USA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037025502","display_name":"James A Blau","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James A Blau","raw_affiliation_strings":["Department of Microbiology and Immunology, UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100731112","display_name":"Haopeng Wang","orcid":"https://orcid.org/0000-0002-4995-0838","observed_orcids":["https://orcid.org/0000-0002-4995-0838"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]},{"id":"https://openalex.org/I4405273141","display_name":"Russell/Engleman Rheumatology Research Center","ror":"https://ror.org/05bf1vc84","country_code":"US","type":"facility","lineage":["https://openalex.org/I4405273141"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Haopeng Wang","raw_affiliation_strings":["Departments of Medicine and of Microbiology & Immunology, the Rosalind Russell-Ephraim P. Engleman Medical Research Center for Arthritis, and the Howard Hughes Medical Institute, University of California, San Francisco, CA 94143","Departments of Medicine and of Microbiology & Immunology, the Rosalind Russell-Ephraim P. Engleman Medical Research Center for Arthritis, and the Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, 94143, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Medicine and of Microbiology & Immunology, the Rosalind Russell-Ephraim P. Engleman Medical Research Center for Arthritis, and the Howard Hughes Medical Institute, University of California, San Francisco, CA 94143","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I180670191","https://openalex.org/I4405273141"]},{"raw_affiliation_string":"Departments of Medicine and of Microbiology & Immunology, the Rosalind Russell-Ephraim P. Engleman Medical Research Center for Arthritis, and the Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, 94143, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I180670191","https://openalex.org/I4405273141"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058484067","display_name":"Noah Zaitlen","orcid":"https://orcid.org/0000-0002-3553-3670","observed_orcids":["https://orcid.org/0000-0002-3553-3670"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Noah Zaitlen","raw_affiliation_strings":["Department of Medicine, Lung Biology Center, University of California, San Francisco, San Francisco, California, USA","Department of Medicine, Lung Biology Center, University of California, San Francisco, San Francisco, 94143, CA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Lung Biology Center, University of California, San Francisco, San Francisco, California, USA","institution_ids":["https://openalex.org/I180670191"]},{"raw_affiliation_string":"Department of Medicine, Lung Biology Center, University of California, San Francisco, San Francisco, 94143, CA, USA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039780162","display_name":"Michael T. McManus","orcid":"https://orcid.org/0000-0003-3013-6569","observed_orcids":["https://orcid.org/0000-0003-3013-6569"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael T McManus","raw_affiliation_strings":["Department of Microbiology and Immunology, UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology and Immunology, UCSF Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA","institution_ids":["https://openalex.org/I180670191"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":2145,"currency":"USD","value_usd":2145},"apc_paid":{"value":2145,"currency":"USD","value_usd":2145},"fwci":0.7377,"has_fulltext":true,"cited_by_count":17,"citation_normalized_percentile":{"value":0.6596634,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":98},"biblio":{"volume":"20","issue":"1","first_page":"107","last_page":"107"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12820","display_name":"Chromatin Remodeling and Cancer","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cell-heterogeneity","display_name":"cellular heterogeneity","score":0.9559999704360962},{"id":"https://openalex.org/keywords/crispr-screening","display_name":"CRISPR screen","score":0.7960000038146973},{"id":"https://openalex.org/keywords/sgrna-library","display_name":"sgRNA library","score":0.8140000104904175},{"id":"https://openalex.org/keywords/clonal-cell-lines","display_name":"clonal cell lines","score":0.9750000238418579},{"id":"https://openalex.org/keywords/growth-kinetics","display_name":"growth kinetics","score":0.8320000171661377},{"id":"https://openalex.org/keywords/treatment-response","display_name":"treatment response","score":0.9350000023841858}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.844784677028656},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.6771909594535828},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5402706265449524},{"id":"https://openalex.org/C64618202","wikidata":"https://www.wikidata.org/wiki/Q3733697","display_name":"Genetic heterogeneity","level":4,"score":0.5381188988685608},{"id":"https://openalex.org/C138673069","wikidata":"https://www.wikidata.org/wiki/Q322229","display_name":"Tracing","level":2,"score":0.46785953640937805},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.4497123956680298},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4217369556427002},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3621237576007843},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.17830970883369446},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.06305226683616638},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.0},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005820","descriptor_name":"Genetic Testing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019169","descriptor_name":"Jurkat Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D058889","descriptor_name":"Molecular Typing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":6,"locations":[{"id":"doi:10.1186/s12864-019-5480-0","doi":"https://doi.org/10.1186/s12864-019-5480-0","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12864-019-5480-0","pdf_url":"https://bmcgenomics.biomedcentral.com/counter/pdf/10.1186/s12864-019-5480-0","source":{"id":"https://openalex.org/S28614077","display_name":"BMC Genomics","issn_l":"1471-2164","issn":["1471-2164"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Genomics","raw_type":"journal-article"},{"id":"doi:10.1101/224345","doi":"https://doi.org/10.1101/224345","is_oa":true,"landing_page_url":"https://doi.org/10.1101/224345","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2017/11/26/224345.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:europepmc.org:5362688","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6364396","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:6364396","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6364396","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Genomics","raw_type":"Text"},{"id":"pmid:30727954","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30727954","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC genomics","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:36504d473df9495196bf6eb1d48e243e","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/36504d473df9495196bf6eb1d48e243e","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Genomics, Vol 20, Iss 1, Pp 1-9 (2019)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1186/s12864-019-5480-0","doi":"https://doi.org/10.1186/s12864-019-5480-0","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12864-019-5480-0","pdf_url":"https://bmcgenomics.biomedcentral.com/counter/pdf/10.1186/s12864-019-5480-0","source":{"id":"https://openalex.org/S28614077","display_name":"BMC Genomics","issn_l":"1471-2164","issn":["1471-2164"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Genomics","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1894139842","display_name":"Richer Models of Asthma Risk:  Bridging the Environment-Genetics Divide","funder_award_id":"K25HL121295","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"},{"id":"https://openalex.org/G3491141739","display_name":"Unlocking the Hidden Kinome","funder_award_id":"U01MH105028","funder_id":"https://openalex.org/F4320337346","funder_display_name":"National Institute of Mental Health"},{"id":"https://openalex.org/G7465630067","display_name":"\u57fa\u4e8e\u78f7\u9178\u5316\u86cb\u767d\u8d28\u7ec4\u5b66\u53caCRISPR\u9057\u4f20\u7b5b\u9009\u6280\u672f\u5bf9CD28\u5171\u523a\u6fc0\u5206\u5b50\u673a\u5236\u7684\u7814\u7a76","funder_award_id":"31670919","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G8376593020","display_name":"Bay Area Cancer Target Discovery and Development Network","funder_award_id":"U01CA168370","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320308528","display_name":"American Foundation for Pharmaceutical Education","ror":"https://ror.org/006dn3r34"},{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"},{"id":"https://openalex.org/F4320337346","display_name":"National Institute of Mental Health","ror":"https://ror.org/04xeg9z08"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2952298077.pdf","grobid_xml":"https://content.openalex.org/works/W2952298077.grobid-xml"},"referenced_works_count":17,"referenced_works":["https://openalex.org/W1486227004","https://openalex.org/W1594078235","https://openalex.org/W1974934611","https://openalex.org/W1982873976","https://openalex.org/W2026592619","https://openalex.org/W2062496875","https://openalex.org/W2100122648","https://openalex.org/W2131395804","https://openalex.org/W2152505144","https://openalex.org/W2292399321","https://openalex.org/W2342536142","https://openalex.org/W2765734704","https://openalex.org/W2772524563","https://openalex.org/W2783339841","https://openalex.org/W2891107038","https://openalex.org/W2949972068","https://openalex.org/W2953135007"],"related_works":["https://openalex.org/W6977306342","https://openalex.org/W6977135195","https://openalex.org/W6977699482","https://openalex.org/W6976725653","https://openalex.org/W4405419739","https://openalex.org/W2765734704","https://openalex.org/W4385718412","https://openalex.org/W4212850078","https://openalex.org/W4390244727","https://openalex.org/W3083204068"],"abstract_inverted_index":{"BACKGROUND:":[0],"While":[1],"pooled":[2,135],"loss-":[3],"and":[4,84,102,124],"gain-of-function":[5],"CRISPR":[6],"screening":[7],"approaches":[8],"have":[9,24],"become":[10],"increasingly":[11],"popular":[12],"to":[13,63,71],"systematically":[14],"investigate":[15],"mammalian":[16],"gene":[17],"function,":[18],"the":[19,29,44,72,96,110,132],"large":[20],"majority":[21],"of":[22,31,46,53,78,87,104,107,127,134],"them":[23],"thus":[25],"far":[26],"not":[27],"investigated":[28],"influence":[30],"cellular":[32],"heterogeneity":[33,120],"on":[34],"screen":[35],"results.":[36],"Instead":[37],"most":[38],"screens":[39],"are":[40],"analyzed":[41],"by":[42],"averaging":[43],"abundance":[45],"perturbed":[47],"cells":[48,108],"from":[49],"a":[50],"bulk":[51],"population":[52],"cells.":[54],"RESULTS:":[55],"Here":[56],"we":[57,117],"developed":[58],"multi-level":[59],"barcoded":[60],"sgRNA":[61],"libraries":[62],"trace":[64],"multiple":[65],"clonal":[66,89,128],"Cas9":[67],"cell":[68,90,129],"lines":[69,91,130],"exposed":[70],"same":[73,111],"environment.":[74],"The":[75],"first":[76],"level":[77,98],"barcoding":[79],"allows":[80],"monitoring":[81],"growth":[82,122],"kinetics":[83,123],"treatment":[85,125],"responses":[86],"multiplexed":[88],"under":[92],"identical":[93],"conditions":[94],"while":[95],"second":[97],"enables":[99],"in-sample":[100],"replication":[101],"tracing":[103],"sub-clonal":[105],"lineages":[106],"expressing":[109],"sgRNA.":[112],"CONCLUSION:":[113],"Using":[114],"our":[115],"approach,":[116],"illustrate":[118],"how":[119],"in":[121],"response":[126],"impairs":[131],"results":[133],"genetic":[136],"screens.":[137]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2019-06-27T00:00:00"}