{"id":"https://openalex.org/W2951705053","doi":"https://doi.org/10.1002/btpr.2868","title":"Derivation and identification of a mechanistic model for a branched enzyme\u2010catalyzed carboligation","display_name":"Derivation and identification of a mechanistic model for a branched enzyme\u2010catalyzed carboligation","publication_year":2019,"publication_date":"2019-06-17","ids":{"openalex":"https://openalex.org/W2951705053","doi":"https://doi.org/10.1002/btpr.2868","mag":"2951705053","pmid":"https://pubmed.ncbi.nlm.nih.gov/31207120"},"language":"en","primary_location":{"id":"doi:10.1002/btpr.2868","doi":"https://doi.org/10.1002/btpr.2868","is_oa":false,"landing_page_url":"https://doi.org/10.1002/btpr.2868","pdf_url":null,"source":{"id":"https://openalex.org/S186451451","display_name":"Biotechnology Progress","issn_l":"1520-6033","issn":["1520-6033","8756-7938"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Progress","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5030722012","display_name":"R\u00fcdiger Ohs","orcid":"https://orcid.org/0000-0002-1805-4440","observed_orcids":["https://orcid.org/0000-0002-1805-4440"]},"institutions":[{"id":"https://openalex.org/I4210102767","display_name":"DWI \u2013 Leibniz Institute for Interactive Materials","ror":"https://ror.org/0186h8060","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210102767"]},{"id":"https://openalex.org/I887968799","display_name":"RWTH Aachen University","ror":"https://ror.org/04xfq0f34","country_code":"DE","type":"education","lineage":["https://openalex.org/I887968799"]},{"id":"https://openalex.org/I94509681","display_name":"Technische Universit\u00e4t Braunschweig","ror":"https://ror.org/010nsgg66","country_code":"DE","type":"education","lineage":["https://openalex.org/I94509681"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"R\u00fcdiger Ohs","raw_affiliation_strings":["Aachener Verfahrenstechnik\u2014Enzyme Process Technology RWTH Aachen University  Aachen Germany","DWI\u2014Leibniz Institute for Interactive Materials Research  Aachen Germany","Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig, Braunschweig, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Aachener Verfahrenstechnik\u2014Enzyme Process Technology RWTH Aachen University  Aachen Germany","institution_ids":["https://openalex.org/I887968799"]},{"raw_affiliation_string":"DWI\u2014Leibniz Institute for Interactive Materials Research  Aachen Germany","institution_ids":["https://openalex.org/I4210102767"]},{"raw_affiliation_string":"Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig, Braunschweig, Germany","institution_ids":["https://openalex.org/I94509681"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Konrad Fischer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I887968799","display_name":"RWTH Aachen University","ror":"https://ror.org/04xfq0f34","country_code":"DE","type":"education","lineage":["https://openalex.org/I887968799"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Konrad Fischer","raw_affiliation_strings":["Aachener Verfahrenstechnik\u2014Enzyme Process Technology RWTH Aachen University  Aachen Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Aachener Verfahrenstechnik\u2014Enzyme Process Technology RWTH Aachen University  Aachen Germany","institution_ids":["https://openalex.org/I887968799"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049659000","display_name":"Marie Sch\u00f6pping","orcid":"https://orcid.org/0000-0003-0929-7552","observed_orcids":["https://orcid.org/0000-0003-0929-7552"]},"institutions":[{"id":"https://openalex.org/I887968799","display_name":"RWTH Aachen University","ror":"https://ror.org/04xfq0f34","country_code":"DE","type":"education","lineage":["https://openalex.org/I887968799"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Marie Sch\u00f6pping","raw_affiliation_strings":["Aachener Verfahrenstechnik\u2014Enzyme Process Technology RWTH Aachen University  Aachen Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Aachener Verfahrenstechnik\u2014Enzyme Process Technology RWTH Aachen University  Aachen Germany","institution_ids":["https://openalex.org/I887968799"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062621205","display_name":"Antje C. Spie\u00df","orcid":"https://orcid.org/0000-0002-8049-8931","observed_orcids":["https://orcid.org/0000-0002-8049-8931"]},"institutions":[{"id":"https://openalex.org/I4210102767","display_name":"DWI \u2013 Leibniz Institute for Interactive Materials","ror":"https://ror.org/0186h8060","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210102767"]},{"id":"https://openalex.org/I887968799","display_name":"RWTH Aachen University","ror":"https://ror.org/04xfq0f34","country_code":"DE","type":"education","lineage":["https://openalex.org/I887968799"]},{"id":"https://openalex.org/I94509681","display_name":"Technische Universit\u00e4t Braunschweig","ror":"https://ror.org/010nsgg66","country_code":"DE","type":"education","lineage":["https://openalex.org/I94509681"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Antje C. Spiess","raw_affiliation_strings":["Aachener Verfahrenstechnik\u2014Enzyme Process Technology RWTH Aachen University  Aachen Germany","DWI\u2014Leibniz Institute for Interactive Materials Research  Aachen Germany","Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig, Braunschweig, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Aachener Verfahrenstechnik\u2014Enzyme Process Technology RWTH Aachen University  Aachen Germany","institution_ids":["https://openalex.org/I887968799"]},{"raw_affiliation_string":"DWI\u2014Leibniz Institute for Interactive Materials Research  Aachen Germany","institution_ids":["https://openalex.org/I4210102767"]},{"raw_affiliation_string":"Institute of Biochemical Engineering Technische Universit\u00e4t Braunschweig, Braunschweig, Germany","institution_ids":["https://openalex.org/I94509681"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5062621205"],"corresponding_institution_ids":["https://openalex.org/I4210102767","https://openalex.org/I887968799","https://openalex.org/I94509681"],"apc_list":{"value":3500,"currency":"USD","value_usd":3500},"apc_paid":null,"fwci":0.2183,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.42453276,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"35","issue":"6","first_page":"e2868","last_page":"e2868"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.8682000041007996,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.8682000041007996,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Physical Organic Chemistry and Reaction Kinetics","score":0.026799999177455902,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.018400000408291817,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5910000205039978},{"id":"https://openalex.org/keywords/mechanistic-kinetic-model","display_name":"mechanistic kinetic model","score":0.7260000109672546},{"id":"https://openalex.org/keywords/ping-pong-bi-bi-mechanism","display_name":"ping-pong bi-bi mechanism","score":0.9679999947547913},{"id":"https://openalex.org/keywords/benzaldehyde-lyase","display_name":"benzaldehyde lyase","score":0.7620000243186951},{"id":"https://openalex.org/keywords/thiamine-diphosphate-dependent-enzymes","display_name":"thiamine diphosphate-dependent enzymes","score":0.828000009059906},{"id":"https://openalex.org/keywords/pseudomonas-fluorescens","display_name":"Pseudomonas fluorescens","score":0.9160000085830688},{"id":"https://openalex.org/keywords/carboligation","display_name":"carboligation","score":0.8159999847412109},{"id":"https://openalex.org/keywords/benzaldehyde","display_name":"benzaldehyde","score":0.8259999752044678},{"id":"https://openalex.org/keywords/propanal","display_name":"propionaldehyde","score":0.9890000224113464},{"id":"https://openalex.org/keywords/enantioselective-biocatalysis","display_name":"enantioselective biocatalysis","score":0.8519999980926514}],"concepts":[{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.6373945474624634},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5603499412536621},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.5141053795814514},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4799647331237793},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3826597034931183},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.37659358978271484},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.32163017988204956},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.15453583002090454}],"mesh":[{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D002384","descriptor_name":"Catalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004798","descriptor_name":"Enzymes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011551","descriptor_name":"Pseudomonas fluorescens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011551","descriptor_name":"Pseudomonas fluorescens","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013237","descriptor_name":"Stereoisomerism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015061","descriptor_name":"1,4-alpha-Glucan Branching Enzyme","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1002/btpr.2868","doi":"https://doi.org/10.1002/btpr.2868","is_oa":false,"landing_page_url":"https://doi.org/10.1002/btpr.2868","pdf_url":null,"source":{"id":"https://openalex.org/S186451451","display_name":"Biotechnology Progress","issn_l":"1520-6033","issn":["1520-6033","8756-7938"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Progress","raw_type":"journal-article"},{"id":"pmid:31207120","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31207120","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology progress","raw_type":"Journal Article"},{"id":"pmh:oai:publications.rwth-aachen.de:787123","doi":null,"is_oa":false,"landing_page_url":"https://publications.rwth-aachen.de/record/787123","pdf_url":null,"source":{"id":"https://openalex.org/S4306401362","display_name":"RWTH Publications (RWTH Aachen)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I887968799","host_organization_name":"RWTH Aachen University","host_organization_lineage":["https://openalex.org/I887968799"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biotechnology progress 35(6), 2868 (2019). doi:10.1002/btpr.2868","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6654725357","display_name":null,"funder_award_id":"FOR 1296","funder_id":"https://openalex.org/F4320320879","funder_display_name":"Deutsche Forschungsgemeinschaft"}],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":52,"referenced_works":["https://openalex.org/W618616555","https://openalex.org/W1044562817","https://openalex.org/W1775008134","https://openalex.org/W1964202435","https://openalex.org/W1969952818","https://openalex.org/W1971502381","https://openalex.org/W1978349142","https://openalex.org/W1986457351","https://openalex.org/W1989527025","https://openalex.org/W1994024442","https://openalex.org/W1998382576","https://openalex.org/W1999832381","https://openalex.org/W2003195392","https://openalex.org/W2013097178","https://openalex.org/W2016179523","https://openalex.org/W2028635434","https://openalex.org/W2031434350","https://openalex.org/W2036407535","https://openalex.org/W2046371360","https://openalex.org/W2046797984","https://openalex.org/W2049020763","https://openalex.org/W2058899256","https://openalex.org/W2059246518","https://openalex.org/W2060056497","https://openalex.org/W2069097572","https://openalex.org/W2070600291","https://openalex.org/W2078155430","https://openalex.org/W2085157281","https://openalex.org/W2090688708","https://openalex.org/W2091007335","https://openalex.org/W2096964295","https://openalex.org/W2097877847","https://openalex.org/W2103239490","https://openalex.org/W2110424789","https://openalex.org/W2111652881","https://openalex.org/W2115725096","https://openalex.org/W2126381540","https://openalex.org/W2128635872","https://openalex.org/W2130131457","https://openalex.org/W2147038637","https://openalex.org/W2165491265","https://openalex.org/W2168648059","https://openalex.org/W2168910593","https://openalex.org/W2255730430","https://openalex.org/W2318277125","https://openalex.org/W2403087393","https://openalex.org/W2621153170","https://openalex.org/W2805130931","https://openalex.org/W2857337405","https://openalex.org/W2949345715","https://openalex.org/W4246090862","https://openalex.org/W4285272180"],"related_works":["https://openalex.org/W2147038637","https://openalex.org/W6963312545","https://openalex.org/W7020816763","https://openalex.org/W2805130931","https://openalex.org/W2028354486","https://openalex.org/W2000916659","https://openalex.org/W392324949","https://openalex.org/W1975665135","https://openalex.org/W2499873202","https://openalex.org/W2999177932"],"abstract_inverted_index":{"The":[0],"kinetic":[1,18,160],"description":[2],"of":[3,24,43,54,112,119],"enzyme-catalyzed":[4],"reactions":[5,34],"is":[6],"a":[7,48,95,100,137,149],"core":[8],"task":[9],"in":[10],"biotechnology":[11],"and":[12,79,121,156],"biochemical":[13],"engineering.":[14],"In":[15],"particular,":[16],"mechanistic":[17],"models":[19],"help":[20],"from":[21,47,131],"the":[22,25,41,70,82,104,108,113,117,125],"discovery":[23],"biocatalyst":[26],"throughout":[27],"its":[28],"application.":[29],"Chemo-":[30],"or":[31],"enantioselective":[32],"enzyme":[33,128],"often":[35],"undergo":[36],"two":[37,44,105],"alternative":[38],"pathways":[39,78],"for":[40,75,85,99],"release":[42],"different":[45],"products":[46,106],"central":[49,110],"intermediate.":[50],"For":[51],"these":[52],"types":[53],"reaction,":[55],"no":[56],"explicit":[57],"reaction":[58,77,102,114,150],"equations":[59],"have":[60],"been":[61],"derived":[62],"so":[63],"far.":[64],"To":[65],"this":[66],"end,":[67],"we":[68,140],"extend":[69],"commonly":[71],"used":[72],"Cleland's":[73],"notation":[74],"branched":[76],"explicitly":[80],"derive":[81],"rate":[83],"expressions":[84],"two-coupled":[86],"ordered":[87],"bi-uni":[88],"reactions.":[89],"This":[90],"mechanism":[91,98,151],"also":[92],"leads":[93,157],"to":[94,145,158],"ping-pong":[96],"bi-bi":[97],"transfer":[101],"between":[103],"via":[107],"same":[109],"intermediate":[111],"system.":[115],"Using":[116],"cross-ligation":[118],"benzaldehyde":[120,129],"propanal":[122],"catalyzed":[123],"by":[124],"thiamine":[126],"diphosphate-dependent":[127],"lyase":[130],"Pseudomonas":[132],"fluorescens":[133],"yielding":[134],"(R)-2-hydroxy-1-phenylbutan-1-one":[135],"as":[136],"case":[138],"study,":[139],"performed":[141],"model-based":[142],"experimental":[143],"analysis":[144],"show":[146],"that":[147],"such":[148],"can":[152],"be":[153],"modeled":[154],"mechanistically":[155],"reasonable":[159],"parameters.":[161]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2019-06-27T00:00:00"}