{"id":"https://openalex.org/W2950945753","doi":"https://doi.org/10.1128/aem.00990-19","title":"A New Suite of Allelic-Exchange Vectors for the Scarless Modification of Proteobacterial Genomes","display_name":"A New Suite of Allelic-Exchange Vectors for the Scarless Modification of Proteobacterial Genomes","publication_year":2019,"publication_date":"2019-06-12","ids":{"openalex":"https://openalex.org/W2950945753","doi":"https://doi.org/10.1128/aem.00990-19","mag":"2950945753","pmid":"https://pubmed.ncbi.nlm.nih.gov/31201277","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6677854"},"language":"en","primary_location":{"id":"doi:10.1128/aem.00990-19","doi":"https://doi.org/10.1128/aem.00990-19","is_oa":false,"landing_page_url":"https://doi.org/10.1128/aem.00990-19","pdf_url":null,"source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2019/05/01/624551.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060871951","display_name":"Jacob E. Lazarus","orcid":"https://orcid.org/0000-0001-9286-3548","observed_orcids":["https://orcid.org/0000-0001-9286-3548"]},"institutions":[{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210087915","display_name":"Massachusetts General Hospital","ror":"https://ror.org/002pd6e78","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210087915","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jacob E. Lazarus","raw_affiliation_strings":["Department of Microbiology, Harvard Medical School, Boston, MA, USA","Division of Infectious Digmseases, Massachusetts General Hospital, Boston, MA, USA","Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, MA, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Digmseases, Massachusetts General Hospital, Boston, MA, USA","institution_ids":["https://openalex.org/I4210087915"]},{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I4210087915"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073133731","display_name":"Alyson R. Warr","orcid":"https://orcid.org/0000-0002-1151-0108","observed_orcids":["https://orcid.org/0000-0002-1151-0108"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alyson R. Warr","raw_affiliation_strings":["Department of Microbiology, Harvard Medical School, Boston, MA, USA","Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Boston, MA, USA","Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, MA, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Boston, MA, USA","institution_ids":["https://openalex.org/I1283280774"]},{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I1283280774"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069707934","display_name":"Carole J. Kuehl","orcid":"https://orcid.org/0000-0002-9608-5533","observed_orcids":["https://orcid.org/0000-0002-9608-5533"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Carole J. Kuehl","raw_affiliation_strings":["Department of Microbiology, Harvard Medical School, Boston, MA, USA","Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Boston, MA, USA","Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, MA, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Boston, MA, USA","institution_ids":["https://openalex.org/I1283280774"]},{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I1283280774"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090978116","display_name":"Rachel T. Giorgio","orcid":"https://orcid.org/0000-0003-2790-0300","observed_orcids":["https://orcid.org/0000-0003-2790-0300"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rachel T. Giorgio","raw_affiliation_strings":["Department of Microbiology, Harvard Medical School, Boston, MA, USA","Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Boston, MA, USA","Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, MA, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Boston, MA, USA","institution_ids":["https://openalex.org/I1283280774"]},{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I1283280774"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017683749","display_name":"Brigid M. Davis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brigid M. Davis","raw_affiliation_strings":["Department of Microbiology, Harvard Medical School, Boston, MA, USA","Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Boston, MA, USA","Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, MA, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Boston, MA, USA","institution_ids":["https://openalex.org/I1283280774"]},{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I1283280774"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067108842","display_name":"Matthew K. Waldor","orcid":"https://orcid.org/0000-0003-1843-7000","observed_orcids":["https://orcid.org/0000-0003-1843-7000"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Matthew K. Waldor","raw_affiliation_strings":["Department of Microbiology, Harvard Medical School, Boston, MA, USA","Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Boston, MA, USA","Howard Hughes Medical Institute, Boston, MA, USA","Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA","Howard Hughes Medical Institute, Boston, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, MA, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Diseases, Brigham and Women\u2019s Hospital, Boston, MA, USA","institution_ids":["https://openalex.org/I1283280774"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, Boston, MA, USA","institution_ids":["https://openalex.org/I1344073410"]},{"raw_affiliation_string":"Department of Microbiology, Harvard Medical School, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Division of Infectious Diseases, Brigham and Women's Hospital, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I1283280774"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, Boston, Massachusetts, USA","institution_ids":["https://openalex.org/I1344073410"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5067108842"],"corresponding_institution_ids":["https://openalex.org/I1283280774","https://openalex.org/I1344073410","https://openalex.org/I136199984"],"apc_list":null,"apc_paid":null,"fwci":1.6551,"has_fulltext":false,"cited_by_count":53,"citation_normalized_percentile":{"value":0.82434104,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":100},"biblio":{"volume":"85","issue":"16","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.86080002784729,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.86080002784729,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.11490000039339066,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.01720000058412552,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/serratia-marcescens","display_name":"Serratia marcescens","score":0.8709999918937683},{"id":"https://openalex.org/keywords/escherichia-coli-o157-h7","display_name":"Escherichia coli O157:H7","score":0.9860000014305115},{"id":"https://openalex.org/keywords/enterobacter-cloacae","display_name":"Enterobacter cloacae","score":0.9990000128746033},{"id":"https://openalex.org/keywords/shigella-flexneri","display_name":"Shigella flexneri","score":1.0},{"id":"https://openalex.org/keywords/ampc-beta-lactamase","display_name":"AmpC beta-lactamase","score":0.9649999737739563},{"id":"https://openalex.org/keywords/beta-lactam-resistance","display_name":"beta-lactam resistance","score":0.9739999771118164}],"concepts":[{"id":"https://openalex.org/C102744134","wikidata":"https://www.wikidata.org/wiki/Q22280388","display_name":"Homologous recombination","level":3,"score":0.7487624883651733},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.6605599522590637},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6130614876747131},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5325300693511963},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4817883372306824},{"id":"https://openalex.org/C156695909","wikidata":"https://www.wikidata.org/wiki/Q3373825","display_name":"Recombination","level":3,"score":0.4784167408943176},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.20371976494789124}],"mesh":[{"descriptor_ui":"D000483","descriptor_name":"Alleles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008826","descriptor_name":"Microbial Sensitivity Tests","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016680","descriptor_name":"Genome, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020560","descriptor_name":"Proteobacteria","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D020560","descriptor_name":"Proteobacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D020560","descriptor_name":"Proteobacteria","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D047090","descriptor_name":"beta-Lactams","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1128/aem.00990-19","doi":"https://doi.org/10.1128/aem.00990-19","is_oa":false,"landing_page_url":"https://doi.org/10.1128/aem.00990-19","pdf_url":null,"source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},{"id":"doi:10.1101/624551","doi":"https://doi.org/10.1101/624551","is_oa":true,"landing_page_url":"https://doi.org/10.1101/624551","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2019/05/01/624551.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:31201277","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31201277","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and environmental microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6677854","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC6677854/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1101/624551","doi":"https://doi.org/10.1101/624551","is_oa":true,"landing_page_url":"https://doi.org/10.1101/624551","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2019/05/01/624551.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.428600013256073,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G5952465375","display_name":"Bacteriology PhD Training Program","funder_award_id":"T32AI132120","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G9000893749","display_name":"Host-pathogen interactions in experimental cholera","funder_award_id":"R01AI042347","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G9000907884","display_name":"Infectious Disease and Basic Microbiological Mechanisms","funder_award_id":"T32AI007061","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320306082","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90"},{"id":"https://openalex.org/F4320309673","display_name":"Harvard Catalyst","ror":null},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2950945753.pdf","grobid_xml":"https://content.openalex.org/works/W2950945753.grobid-xml"},"referenced_works_count":47,"referenced_works":["https://openalex.org/W1606347898","https://openalex.org/W1787690277","https://openalex.org/W1870558638","https://openalex.org/W1894520368","https://openalex.org/W1908889304","https://openalex.org/W1967624732","https://openalex.org/W1968139814","https://openalex.org/W1979973356","https://openalex.org/W1985378043","https://openalex.org/W1991295288","https://openalex.org/W1996929743","https://openalex.org/W2000046950","https://openalex.org/W2003213412","https://openalex.org/W2004850153","https://openalex.org/W2018645639","https://openalex.org/W2033090854","https://openalex.org/W2041215841","https://openalex.org/W2080233018","https://openalex.org/W2088651920","https://openalex.org/W2099303284","https://openalex.org/W2107194730","https://openalex.org/W2116443594","https://openalex.org/W2123467717","https://openalex.org/W2123712967","https://openalex.org/W2125957234","https://openalex.org/W2159487602","https://openalex.org/W2169639178","https://openalex.org/W2171376468","https://openalex.org/W2181192066","https://openalex.org/W2260281189","https://openalex.org/W2292458301","https://openalex.org/W2343292289","https://openalex.org/W2416967930","https://openalex.org/W2461296320","https://openalex.org/W2512657363","https://openalex.org/W2594224298","https://openalex.org/W2761401193","https://openalex.org/W2793434096","https://openalex.org/W2799524357","https://openalex.org/W2802577608","https://openalex.org/W2806551567","https://openalex.org/W2895807786","https://openalex.org/W2902073703","https://openalex.org/W2921330592","https://openalex.org/W2969197547","https://openalex.org/W2989466399","https://openalex.org/W3172911918"],"related_works":["https://openalex.org/W3123502856","https://openalex.org/W7000578456","https://openalex.org/W2132451297","https://openalex.org/W2109989100","https://openalex.org/W7098803093","https://openalex.org/W2181192066","https://openalex.org/W2095519853","https://openalex.org/W1999046178","https://openalex.org/W2585461254","https://openalex.org/W4388901758"],"abstract_inverted_index":{"Targeted":[0],"modification":[1],"of":[2,10,46,68,81,88,98,100],"bacterial":[3],"genomes":[4],"is":[5,14],"critical":[6],"for":[7,38,77],"genetic":[8],"analysis":[9],"microorganisms.":[11],"Allelic":[12],"exchange":[13],"a":[15,44],"technique":[16],"that":[17,64],"relies":[18],"on":[19,105],"homologous":[20,40,82],"recombination":[21],"to":[22,94],"replace":[23],"native":[24],"loci":[25],"with":[26],"engineered":[27],"sequences.":[28],"However,":[29],"current":[30],"allelic-exchange":[31,48],"vectors":[32,72,90],"often":[33],"enable":[34,65],"only":[35],"weak":[36],"selection":[37,76],"successful":[39],"recombination.":[41,83],"We":[42,84],"developed":[43],"suite":[45],"new":[47],"vectors,":[49],"pTOX,":[50],"which":[51],"were":[52],"validated":[53],"in":[54],"several":[55],"medically":[56],"important":[57],"proteobacteria.":[58],"They":[59],"encode":[60],"visible":[61],"nonfluorescent":[62],"chromoproteins":[63],"easy":[66],"identification":[67],"colonies":[69],"bearing":[70],"integrated":[71],"and":[73],"permit":[74],"stringent":[75],"the":[78,86,96],"second":[79],"step":[80],"demonstrate":[85],"utility":[87],"these":[89],"by":[91],"using":[92],"them":[93],"investigate":[95],"effect":[97],"inactivation":[99],"Serratia":[101],"marcescens":[102],"peptidoglycan":[103],"amidohydrolases":[104],"\u03b2-lactam":[106],"antibiotic":[107],"resistance.":[108]},"counts_by_year":[{"year":2026,"cited_by_count":12},{"year":2025,"cited_by_count":16},{"year":2024,"cited_by_count":8},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":2}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2019-06-27T00:00:00"}
