{"id":"https://openalex.org/W2948034354","doi":"https://doi.org/10.1101/pdb.prot095224","title":"Screening Colonies by Polymerase Chain Reaction (PCR)","display_name":"Screening Colonies by Polymerase Chain Reaction (PCR)","publication_year":2019,"publication_date":"2019-06-01","ids":{"openalex":"https://openalex.org/W2948034354","doi":"https://doi.org/10.1101/pdb.prot095224","mag":"2948034354","pmid":"https://pubmed.ncbi.nlm.nih.gov/31160384"},"language":"en","primary_location":{"id":"doi:10.1101/pdb.prot095224","doi":"https://doi.org/10.1101/pdb.prot095224","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot095224","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},"volume":null,"type":"preprint","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060605798","display_name":"Michael R. Green","orcid":"https://orcid.org/0000-0003-3017-3298","observed_orcids":["https://orcid.org/0000-0003-3017-3298","https://orcid.org/0000-0001-6309-9472"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Michael R. Green","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5112152140","display_name":"Joseph Sambrook","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joseph Sambrook","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"2019","issue":"6","first_page":"pdb.prot095224","last_page":"pdb.prot095224"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9848999977111816,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9848999977111816,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.010300000198185444,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0032999999821186066,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/colony-screening","display_name":"colony screening","score":0.8610000014305115},{"id":"https://openalex.org/keywords/pcr-based-screening","display_name":"PCR-based screening","score":0.6019999980926514},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8510000109672546},{"id":"https://openalex.org/keywords/recombinant-plasmid","display_name":"recombinant plasmid","score":0.9760000109672546},{"id":"https://openalex.org/keywords/primer-design","display_name":"primer design","score":0.5569999814033508},{"id":"https://openalex.org/keywords/cloning","display_name":"cloning","score":0.32899999618530273}],"concepts":[{"id":"https://openalex.org/C2780098074","wikidata":"https://www.wikidata.org/wiki/Q6037362","display_name":"Insert (composites)","level":2,"score":0.8260436654090881},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.801117479801178},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.7806323766708374},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.6682756543159485},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6059393286705017},{"id":"https://openalex.org/C20682886","wikidata":"https://www.wikidata.org/wiki/Q475229","display_name":"Inverse polymerase chain reaction","level":5,"score":0.5638088583946228},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5510169863700867},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5147569179534912},{"id":"https://openalex.org/C200658500","wikidata":"https://www.wikidata.org/wiki/Q4112082","display_name":"In silico PCR","level":5,"score":0.5094078183174133},{"id":"https://openalex.org/C52001828","wikidata":"https://www.wikidata.org/wiki/Q2902493","display_name":"Primer dimer","level":5,"score":0.4974086582660675},{"id":"https://openalex.org/C186008079","wikidata":"https://www.wikidata.org/wiki/Q7226557","display_name":"Polymerase chain reaction optimization","level":5,"score":0.4807312488555908},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4722927212715149},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.45676612854003906},{"id":"https://openalex.org/C112456955","wikidata":"https://www.wikidata.org/wiki/Q4118048","display_name":"Hot start PCR","level":5,"score":0.4348203241825104},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.42864862084388733},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3522581458091736},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34767574071884155},{"id":"https://openalex.org/C157431215","wikidata":"https://www.wikidata.org/wiki/Q856465","display_name":"Nested polymerase chain reaction","level":4,"score":0.22506016492843628},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.158917635679245},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.13558301329612732},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.051838189363479614},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015169","descriptor_name":"Colony Count, Microbial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1101/pdb.prot095224","doi":"https://doi.org/10.1101/pdb.prot095224","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot095224","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},{"id":"pmid:31160384","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31160384","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor protocols","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":2,"referenced_works":["https://openalex.org/W2080460613","https://openalex.org/W2800731892"],"related_works":["https://openalex.org/W2381940894","https://openalex.org/W2037690049","https://openalex.org/W2374560572","https://openalex.org/W2416393887","https://openalex.org/W2540784042","https://openalex.org/W4244898637","https://openalex.org/W2416816954","https://openalex.org/W117222535","https://openalex.org/W2248580222","https://openalex.org/W2379970767"],"abstract_inverted_index":{"Polymerase":[0],"chain":[1],"reaction":[2,43],"(PCR),":[3],"rather":[4],"than":[5],"the":[6,36,41,51,54,66,72,78],"more":[7],"labor-intensive":[8],"minipreps,":[9],"is":[10],"often":[11],"used":[12,39,46,62],"to":[13,65,71],"screen":[14],"colonies":[15,28],"of":[16,24,53],"Escherichia":[17],"coli":[18],"for":[19,29,47],"recombinant":[20],"plasmids":[21],"containing":[22],"sequences":[23,68],"interest.":[25],"When":[26],"screening":[27],"a":[30,57],"DNA":[31],"fragment":[32],"amplified":[33],"by":[34],"PCR,":[35],"same":[37],"primers":[38],"in":[40],"amplification":[42],"can":[44,60],"be":[45,61],"screening.":[48],"To":[49],"discover":[50],"orientation":[52],"cloned":[55],"fragment,":[56],"third":[58],"primer":[59],"that":[63],"binds":[64],"plasmid":[67],"immediately":[69],"adjacent":[70],"insertion":[73],"site":[74],"and":[75],"points":[76],"toward":[77],"insert.":[79]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":2},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2019-06-14T00:00:00"}
