{"id":"https://openalex.org/W2947525246","doi":null,"title":"Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology.","display_name":"Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology.","publication_year":2019,"publication_date":"2019-04-01","ids":{"openalex":"https://openalex.org/W2947525246","mag":"2947525246","pmid":"https://pubmed.ncbi.nlm.nih.gov/31057718","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6490408"},"language":"en","primary_location":{"id":"pmh:oai:europepmc.org:5484262","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6490408","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6490408","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5067891082","display_name":"Hamze Zare","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114982161","display_name":"Isfahan University of Medical Sciences","ror":"https://ror.org/04waqzz56","country_code":"IR","type":"education","lineage":["https://openalex.org/I114982161"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Hamze Zare","raw_affiliation_strings":["Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran","institution_ids":["https://openalex.org/I114982161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078237657","display_name":"M. Hamid","orcid":"https://orcid.org/0000-0002-4834-4502","observed_orcids":["https://orcid.org/0000-0002-4834-4502"]},"institutions":[{"id":"https://openalex.org/I114982161","display_name":"Isfahan University of Medical Sciences","ror":"https://ror.org/04waqzz56","country_code":"IR","type":"education","lineage":["https://openalex.org/I114982161"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Hamid Mir Mohammad Sadeghi","raw_affiliation_strings":["Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran","institution_ids":["https://openalex.org/I114982161"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5038132020","display_name":"Vajihe Akbari","orcid":"https://orcid.org/0000-0003-0754-1486","observed_orcids":["https://orcid.org/0000-0003-0754-1486"]},"institutions":[{"id":"https://openalex.org/I114982161","display_name":"Isfahan University of Medical Sciences","ror":"https://ror.org/04waqzz56","country_code":"IR","type":"education","lineage":["https://openalex.org/I114982161"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Vajihe Akbari","raw_affiliation_strings":["Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmaceutical Biotechnology and Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran","institution_ids":["https://openalex.org/I114982161"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I114982161"],"apc_list":null,"apc_paid":null,"fwci":0.4365,"has_fulltext":false,"cited_by_count":17,"citation_normalized_percentile":{"value":0.52818865,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"11","issue":"2","first_page":"162","last_page":"168"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.3953000009059906,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.3953000009059906,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.17910000681877136,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.08720000088214874,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/reteplase","display_name":"reteplase","score":0.9259999990463257},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.9409999847412109},{"id":"https://openalex.org/keywords/escherichia-coli-fermentation","display_name":"Escherichia coli fermentation","score":0.9309999942779541},{"id":"https://openalex.org/keywords/fermentation-optimization","display_name":"fermentation optimization","score":0.8769999742507935},{"id":"https://openalex.org/keywords/response-surface-methodology","display_name":"response surface methodology","score":0.8410000205039978},{"id":"https://openalex.org/keywords/temperature","display_name":"temperature","score":0.5830000042915344},{"id":"https://openalex.org/keywords/ph","display_name":"pH","score":0.7459999918937683},{"id":"https://openalex.org/keywords/shaking-speed","display_name":"shaking speed","score":0.8050000071525574},{"id":"https://openalex.org/keywords/oxygen-supply","display_name":"oxygen supply","score":0.9319999814033508},{"id":"https://openalex.org/keywords/plasmid-instability","display_name":"plasmid instability","score":0.6930000185966492},{"id":"https://openalex.org/keywords/acetate-accumulation","display_name":"acetate accumulation","score":0.8230000138282776},{"id":"https://openalex.org/keywords/oxidative-damage","display_name":"oxidative damage","score":0.652999997138977}],"concepts":[{"id":"https://openalex.org/C150077022","wikidata":"https://www.wikidata.org/wiki/Q3136137","display_name":"Response surface methodology","level":2,"score":0.8148883581161499},{"id":"https://openalex.org/C2780790693","wikidata":"https://www.wikidata.org/wiki/Q3933703","display_name":"Reteplase","level":4,"score":0.7303751111030579},{"id":"https://openalex.org/C168170006","wikidata":"https://www.wikidata.org/wiki/Q864699","display_name":"Bioreactor","level":2,"score":0.7079569101333618},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.6855178475379944},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6574686169624329},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.4796767234802246},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.44024530053138733},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.405615895986557},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.30414944887161255},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.2932155132293701},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.2029733955860138},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08879551291465759},{"id":"https://openalex.org/C15744967","wikidata":"https://www.wikidata.org/wiki/Q9418","display_name":"Psychology","level":0,"score":0.0},{"id":"https://openalex.org/C2779581417","wikidata":"https://www.wikidata.org/wiki/Q1931577","display_name":"Thrombolysis","level":3,"score":0.0},{"id":"https://openalex.org/C500558357","wikidata":"https://www.wikidata.org/wiki/Q12152","display_name":"Myocardial infarction","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C118552586","wikidata":"https://www.wikidata.org/wiki/Q7867","display_name":"Psychiatry","level":1,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"pmh:oai:europepmc.org:5484262","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6490408","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:31057718","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31057718","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Avicenna journal of medical biotechnology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6490408","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC6490408/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Avicenna J Med Biotechnol","raw_type":"Text"},{"id":"mag:2947525246","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31057718","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:europepmc.org:5484262","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6490408","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W926438480","https://openalex.org/W1561253500","https://openalex.org/W1581148528","https://openalex.org/W1969505761","https://openalex.org/W1969932115","https://openalex.org/W1975084573","https://openalex.org/W1979570013","https://openalex.org/W1984119456","https://openalex.org/W1984613385","https://openalex.org/W1987485281","https://openalex.org/W1996822335","https://openalex.org/W1999315378","https://openalex.org/W2005261732","https://openalex.org/W2022418787","https://openalex.org/W2024244058","https://openalex.org/W2033509028","https://openalex.org/W2061429358","https://openalex.org/W2066947166","https://openalex.org/W2073501728","https://openalex.org/W2086429221","https://openalex.org/W2088376206","https://openalex.org/W2093624850","https://openalex.org/W2099179129","https://openalex.org/W2105979475","https://openalex.org/W2110039658","https://openalex.org/W2125648541","https://openalex.org/W2129162353","https://openalex.org/W2131025983","https://openalex.org/W2135213731","https://openalex.org/W2143948627","https://openalex.org/W2165056157","https://openalex.org/W2171421273","https://openalex.org/W2349553011","https://openalex.org/W2625588494","https://openalex.org/W2769232385","https://openalex.org/W2789745026"],"related_works":["https://openalex.org/W7095696772","https://openalex.org/W2093592006","https://openalex.org/W2193144939","https://openalex.org/W1422827474","https://openalex.org/W2098798735","https://openalex.org/W3166627785","https://openalex.org/W2369882885","https://openalex.org/W3157342571","https://openalex.org/W2394158788","https://openalex.org/W2806716583"],"abstract_inverted_index":{"BACKGROUND:":[0],"Expression":[1],"of":[2,18,37,97,101],"heterologous":[3],"proteins":[4],"at":[5,77],"large":[6,78],"scale":[7,79],"is":[8,27],"often":[9],"a":[10],"challenging":[11],"job":[12],"due":[13],"to":[14,29,94],"plasmid":[15],"instability,":[16],"accumulation":[17],"acetate":[19],"and":[20,35,45,47,53,71,80],"oxidative":[21],"damage":[22],"in":[23,74],"bioreactors.":[24],"Therefore,":[25],"it":[26],"necessary":[28],"optimize":[30],"parameters":[31],"influencing":[32],"cell":[33],"growth":[34,52],"expression":[36,76],"recombinant":[38],"protein.":[39],"METHODS:":[40],".,":[41,68],"temperature,":[42],"shaking":[43],"speed":[44],"pH)":[46],"their":[48],"interactions":[49],"with":[50],"cellular":[51],"protein":[54,75],"production.":[55],"The":[56,86],"obtained":[57],"data":[58],"were":[59],"analyzed":[60],"by":[61],"Design":[62],"Expert":[63],"software.":[64],"RESULTS:":[65],".":[66],"CONCLUSION:":[67],"pH,":[69],"temperature":[70],"oxygen":[72],"supply)":[73],"confirm":[81],"the":[82],"need":[83],"for":[84,99],"optimization.":[85],"proposed":[87],"strategy":[88],"here":[89],"can":[90],"also":[91],"be":[92],"applied":[93],"experimental":[95],"set-up":[96],"optimization":[98],"fermentation":[100],"other":[102],"proteins.":[103]},"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2019-06-07T00:00:00"}
