{"id":"https://openalex.org/W2947040147","doi":"https://doi.org/10.1186/s13068-019-1471-2","title":"Visualizing wax ester fermentation in single Euglena gracilis cells by Raman microspectroscopy and multivariate curve resolution analysis","display_name":"Visualizing wax ester fermentation in single Euglena gracilis cells by Raman microspectroscopy and multivariate curve resolution analysis","publication_year":2019,"publication_date":"2019-05-22","ids":{"openalex":"https://openalex.org/W2947040147","doi":"https://doi.org/10.1186/s13068-019-1471-2","mag":"2947040147","pmid":"https://pubmed.ncbi.nlm.nih.gov/31139258","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6529988"},"language":"en","primary_location":{"id":"doi:10.1186/s13068-019-1471-2","doi":"https://doi.org/10.1186/s13068-019-1471-2","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13068-019-1471-2","pdf_url":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-019-1471-2","source":{"id":"https://openalex.org/S137860580","display_name":"Biotechnology for Biofuels","issn_l":"1754-6834","issn":["1754-6834"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology for Biofuels","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-019-1471-2","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086146140","display_name":"Keita Iwasaki","orcid":"https://orcid.org/0000-0002-3908-2272","observed_orcids":["https://orcid.org/0000-0002-3908-2272","https://orcid.org/0009-0000-3628-9297"]},"institutions":[{"id":"https://openalex.org/I4588055","display_name":"Tottori University","ror":"https://ror.org/024yc3q36","country_code":"JP","type":"education","lineage":["https://openalex.org/I4588055"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Keita Iwasaki","raw_affiliation_strings":["1The United Graduate School of Agricultural Sciences, Tottori University, Tottori, 680-8550 Japan","The United Graduate School of Agricultural Sciences, Tottori University, Tottori, 680-8550, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1The United Graduate School of Agricultural Sciences, Tottori University, Tottori, 680-8550 Japan","institution_ids":["https://openalex.org/I4588055"]},{"raw_affiliation_string":"The United Graduate School of Agricultural Sciences, Tottori University, Tottori, 680-8550, Japan","institution_ids":["https://openalex.org/I4588055"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078630116","display_name":"Asuka Kaneko","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I169016828","display_name":"Shimane University","ror":"https://ror.org/01jaaym28","country_code":"JP","type":"education","lineage":["https://openalex.org/I169016828"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Asuka Kaneko","raw_affiliation_strings":["2Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504 Japan","Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504 Japan","institution_ids":["https://openalex.org/I169016828"]},{"raw_affiliation_string":"Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504, Japan","institution_ids":["https://openalex.org/I169016828"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100697389","display_name":"Yuji Tanaka","orcid":"https://orcid.org/0000-0002-2447-8389","observed_orcids":["https://orcid.org/0000-0002-2447-8389"]},"institutions":[{"id":"https://openalex.org/I169016828","display_name":"Shimane University","ror":"https://ror.org/01jaaym28","country_code":"JP","type":"education","lineage":["https://openalex.org/I169016828"]},{"id":"https://openalex.org/I4210086780","display_name":"Japan Science and Technology Agency","ror":"https://ror.org/00097mb19","country_code":"JP","type":"government","lineage":["https://openalex.org/I1319490839","https://openalex.org/I4210086780"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yuji Tanaka","raw_affiliation_strings":["2Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504 Japan","3Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi, 332-0012 Japan","Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504 Japan","institution_ids":["https://openalex.org/I169016828"]},{"raw_affiliation_string":"3Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi, 332-0012 Japan","institution_ids":["https://openalex.org/I4210086780"]},{"raw_affiliation_string":"Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504, Japan","institution_ids":["https://openalex.org/I169016828"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019308992","display_name":"Takahiro Ishikawa","orcid":"https://orcid.org/0000-0002-5834-2922","observed_orcids":["https://orcid.org/0000-0002-5834-2922"]},"institutions":[{"id":"https://openalex.org/I169016828","display_name":"Shimane University","ror":"https://ror.org/01jaaym28","country_code":"JP","type":"education","lineage":["https://openalex.org/I169016828"]},{"id":"https://openalex.org/I4210086780","display_name":"Japan Science and Technology Agency","ror":"https://ror.org/00097mb19","country_code":"JP","type":"government","lineage":["https://openalex.org/I1319490839","https://openalex.org/I4210086780"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takahiro Ishikawa","raw_affiliation_strings":["2Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504 Japan","3Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi, 332-0012 Japan","Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504 Japan","institution_ids":["https://openalex.org/I169016828"]},{"raw_affiliation_string":"3Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi, 332-0012 Japan","institution_ids":["https://openalex.org/I4210086780"]},{"raw_affiliation_string":"Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504, Japan","institution_ids":["https://openalex.org/I169016828"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072045133","display_name":"Hemanth Noothalapati","orcid":"https://orcid.org/0000-0002-8279-7716","observed_orcids":["https://orcid.org/0000-0002-8279-7716"]},"institutions":[{"id":"https://openalex.org/I169016828","display_name":"Shimane University","ror":"https://ror.org/01jaaym28","country_code":"JP","type":"education","lineage":["https://openalex.org/I169016828"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Hemanth Noothalapati","raw_affiliation_strings":["4Raman Project Center for Medical and Biological Applications, Shimane University, Matsue, 690-8504 Japan","Raman Project Center for Medical and Biological Applications, Shimane University, Matsue, 690-8504, Japan"],"raw_orcid":"https://orcid.org/0000-0002-8279-7716","affiliations":[{"raw_affiliation_string":"4Raman Project Center for Medical and Biological Applications, Shimane University, Matsue, 690-8504 Japan","institution_ids":["https://openalex.org/I169016828"]},{"raw_affiliation_string":"Raman Project Center for Medical and Biological Applications, Shimane University, Matsue, 690-8504, Japan","institution_ids":["https://openalex.org/I169016828"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101404265","display_name":"Tatsuyuki Yamamoto","orcid":"https://orcid.org/0000-0002-9156-6944","observed_orcids":["https://orcid.org/0000-0002-9156-6944"]},"institutions":[{"id":"https://openalex.org/I169016828","display_name":"Shimane University","ror":"https://ror.org/01jaaym28","country_code":"JP","type":"education","lineage":["https://openalex.org/I169016828"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Tatsuyuki Yamamoto","raw_affiliation_strings":["2Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504 Japan","4Raman Project Center for Medical and Biological Applications, Shimane University, Matsue, 690-8504 Japan","Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504 Japan","institution_ids":["https://openalex.org/I169016828"]},{"raw_affiliation_string":"4Raman Project Center for Medical and Biological Applications, Shimane University, Matsue, 690-8504 Japan","institution_ids":["https://openalex.org/I169016828"]},{"raw_affiliation_string":"Faculty of Life and Environmental Science, Shimane University, Matsue, 690-8504, Japan","institution_ids":["https://openalex.org/I169016828"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5072045133","https://openalex.org/A5101404265"],"corresponding_institution_ids":["https://openalex.org/I169016828"],"apc_list":{"value":2680,"currency":"USD","value_usd":2680},"apc_paid":{"value":2680,"currency":"USD","value_usd":2680},"fwci":0.8475,"has_fulltext":true,"cited_by_count":19,"citation_normalized_percentile":{"value":0.69362588,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":98},"biblio":{"volume":"12","issue":"1","first_page":"128","last_page":"128"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.95169997215271,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.95169997215271,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10476","display_name":"Algal biology and biofuel production","score":0.02710000053048134,"subfield":{"id":"https://openalex.org/subfields/2105","display_name":"Renewable Energy, Sustainability and the Environment"},"field":{"id":"https://openalex.org/fields/21","display_name":"Energy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10640","display_name":"Spectroscopy and Chemometric Analyses","score":0.010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/euglena-gracilis","display_name":"Euglena gracilis","score":0.9890000224113464},{"id":"https://openalex.org/keywords/micro-raman-spectroscopy","display_name":"micro-Raman spectroscopy","score":0.8539999723434448},{"id":"https://openalex.org/keywords/paramylon","display_name":"paramylon","score":0.8059999942779541},{"id":"https://openalex.org/keywords/microalgal-biofuels","display_name":"microalgal biofuels","score":0.875},{"id":"https://openalex.org/keywords/anaerobic-culture","display_name":"anaerobic culture","score":0.6639999747276306},{"id":"https://openalex.org/keywords/lipid-metabolism","display_name":"lipid metabolism","score":0.722000002861023}],"concepts":[{"id":"https://openalex.org/C2780472036","wikidata":"https://www.wikidata.org/wiki/Q309852","display_name":"Euglena gracilis","level":4,"score":0.9152461886405945},{"id":"https://openalex.org/C2780437362","wikidata":"https://www.wikidata.org/wiki/Q236001","display_name":"Euglena","level":4,"score":0.8224998712539673},{"id":"https://openalex.org/C2993415590","wikidata":"https://www.wikidata.org/wiki/Q862228","display_name":"Raman microspectroscopy","level":3,"score":0.5130159258842468},{"id":"https://openalex.org/C40003534","wikidata":"https://www.wikidata.org/wiki/Q862228","display_name":"Raman spectroscopy","level":2,"score":0.44649824500083923},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3566490411758423},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34511542320251465},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26123374700546265},{"id":"https://openalex.org/C69305403","wikidata":"https://www.wikidata.org/wiki/Q47263","display_name":"Chloroplast","level":3,"score":0.1570211946964264},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.12079799175262451},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.1154557466506958},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.1186/s13068-019-1471-2","doi":"https://doi.org/10.1186/s13068-019-1471-2","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13068-019-1471-2","pdf_url":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-019-1471-2","source":{"id":"https://openalex.org/S137860580","display_name":"Biotechnology for Biofuels","issn_l":"1754-6834","issn":["1754-6834"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology for Biofuels","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6529988","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6529988","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnol Biofuels","raw_type":"Text"},{"id":"pmid:31139258","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31139258","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology for biofuels","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:e61283960bfe4c2199255909b7472df8","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/e61283960bfe4c2199255909b7472df8","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biotechnology for Biofuels, Vol 12, Iss 1, Pp 1-10 (2019)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1186/s13068-019-1471-2","doi":"https://doi.org/10.1186/s13068-019-1471-2","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s13068-019-1471-2","pdf_url":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-019-1471-2","source":{"id":"https://openalex.org/S137860580","display_name":"Biotechnology for Biofuels","issn_l":"1754-6834","issn":["1754-6834"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology for Biofuels","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Affordable and clean energy","score":0.5690000057220459,"id":"https://metadata.un.org/sdg/7"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2521286739","display_name":null,"funder_award_id":"CREST","funder_id":"https://openalex.org/F4320334789","funder_display_name":"Japan Science and Technology Agency"},{"id":"https://openalex.org/G3685370120","display_name":"Development of a low invasive and non-bioptic diagnostic technique for eosinophil esophagitis by Raman spectroscopy","funder_award_id":"19H04486","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G3972057304","display_name":"The development of a Raman spectroscopic technique to diagnose Eosinophil Esophagitis  non-bioptically","funder_award_id":"15H04763","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G6718509927","display_name":null,"funder_award_id":"CREST","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320322543","display_name":"Shimane University","ror":"https://ror.org/01jaaym28"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"},{"id":"https://openalex.org/F4320334789","display_name":"Japan Science and Technology Agency","ror":"https://ror.org/00097mb19"},{"id":"https://openalex.org/F4320338075","display_name":"Core Research for Evolutional Science and Technology","ror":"https://ror.org/00097mb19"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2947040147.pdf","grobid_xml":"https://content.openalex.org/works/W2947040147.grobid-xml"},"referenced_works_count":30,"referenced_works":["https://openalex.org/W1480838520","https://openalex.org/W1507005829","https://openalex.org/W1584420066","https://openalex.org/W1592451644","https://openalex.org/W1975952320","https://openalex.org/W1991296357","https://openalex.org/W2015670561","https://openalex.org/W2030867470","https://openalex.org/W2035481137","https://openalex.org/W2045012993","https://openalex.org/W2046977601","https://openalex.org/W2060012783","https://openalex.org/W2076981712","https://openalex.org/W2084423617","https://openalex.org/W2122437201","https://openalex.org/W2143561090","https://openalex.org/W2147134978","https://openalex.org/W2156778513","https://openalex.org/W2199492564","https://openalex.org/W2412014446","https://openalex.org/W2476246726","https://openalex.org/W2570973228","https://openalex.org/W2586492191","https://openalex.org/W2607150488","https://openalex.org/W2731833551","https://openalex.org/W2743364727","https://openalex.org/W2764327990","https://openalex.org/W2797626384","https://openalex.org/W2807563609","https://openalex.org/W2911072825"],"related_works":["https://openalex.org/W2546971700","https://openalex.org/W2554119837","https://openalex.org/W2019214503","https://openalex.org/W2592293933","https://openalex.org/W1584420066","https://openalex.org/W2594240434","https://openalex.org/W2888565368","https://openalex.org/W2084836238","https://openalex.org/W4407585675","https://openalex.org/W4401447801"],"abstract_inverted_index":{"Global":[0],"demand":[1],"for":[2,33,59,230],"energy":[3],"is":[4,38,92,157,257,266,274,296,303],"on":[5,309],"the":[6,194,231,307,329],"rise":[7],"at":[8],"a":[9,28,97,120,173,209],"time":[10,233],"when":[11],"limited":[12,159],"natural":[13],"resources":[14],"are":[15,24],"fast":[16],"depleting.":[17],"To":[18],"address":[19],"this":[20,292],"issue,":[21],"microalgal":[22],"biofuels":[23],"being":[25],"recommended":[26],"as":[27,57,106,167],"renewable":[29],"and":[30,70,86,108,148,189,268,302],"eco-friendly":[31],"substitute":[32],"fossil":[34],"fuels.":[35],"Euglena":[36,116,133,200,235,248],"gracilis":[37,117],"one":[39],"such":[40,105],"candidate":[41,212],"that":[42,55,262,269,320],"has":[43],"received":[44],"special":[45],"interest":[46],"due":[47],"to":[48,51,66,78,101,127,146,163,172,184,204,214,276,299,305,317,323],"their":[49,150,190],"ability":[50,162],"synthesize":[52],"wax":[53,111,210,250],"esters":[54,198],"serve":[56],"precursors":[58],"production":[60,326],"of":[61,75,193,223,227],"drop-in":[62,216],"jet":[63,217],"fuel.":[64,218],"However,":[65,154],"realize":[67],"economic":[68],"viability":[69],"achieve":[71],"industrial-scale":[72],"production,":[73],"development":[74],"novel":[76],"methods":[77],"characterize":[79],"algal":[80],"cells,":[81],"evaluate":[82],"its":[83,161],"culture":[84],"conditions,":[85],"construct":[87],"appropriate":[88],"genetically":[89],"modified":[90],"strains":[91],"necessary.":[93],"Here,":[94],"we":[95,141,177,220,285],"report":[96],"Raman":[98,125,143,174,254,281],"microspectroscopy-based":[99],"method":[100],"visualize":[102],"important":[103],"metabolites":[104],"paramylon":[107],"ester":[109,112,211,251],"during":[110,249],"fermentation":[113,252],"in":[114,119,132,160,199,234,243,247,291,312,328],"single":[115],"cells":[118],"label-free":[121],"manner.":[122],"We":[123,241],"measured":[124,288],"spectra":[126],"obtain":[128,164,185],"intracellular":[129],"biomolecular":[130],"information":[131,166,188,246,279],"under":[134],"anaerobic":[135],"condition.":[136],"First,":[137],"by":[138,253],"univariate":[139,155,264],"approach,":[140],"identified":[142,203],"markers":[144],"corresponding":[145],"paramylon/esters":[147],"constructed":[149],"time-lapse":[151],"chemical":[152],"images.":[153],"analysis":[156,183,273],"severely":[158],"detailed":[165],"several":[168],"molecules":[169],"can":[170],"contribute":[171],"band.":[175],"Therefore,":[176],"further":[178],"employed":[179],"multivariate":[180,239,270],"curve":[181,271],"resolution":[182,272],"chain":[186],"length-specific":[187],"abundance":[191],"images":[192],"produced":[195],"esters.":[196],"Accumulated":[197],"were":[201],"particularly":[202],"be":[205],"myristyl":[206,228],"myristate":[207,229],"(C28),":[208],"suitable":[213],"prepare":[215],"Interestingly,":[219],"found":[221],"accumulation":[222],"two":[224],"different":[225],"forms":[226],"first":[232],"through":[236],"our":[237,260,294],"exploratory":[238],"analysis.":[240],"succeeded":[242],"visualizing":[244],"molecular-specific":[245],"microspectroscopy.":[255],"It":[256],"obvious":[258],"from":[259,280],"results":[261],"simple":[263],"approach":[265,295],"insufficient":[267],"crucial":[275],"extract":[277],"hidden":[278],"spectra.":[282],"Even":[283],"though":[284],"have":[286],"not":[287],"any":[289],"mutants":[290],"study,":[293],"directly":[297],"applicable":[298],"other":[300],"systems":[301],"expected":[304],"deepen":[306],"knowledge":[308],"lipid":[310],"metabolism":[311],"microalgae,":[313],"which":[314],"eventually":[315],"leads":[316],"new":[318],"strategies":[319],"will":[321],"help":[322],"enhance":[324],"biofuel":[325],"efficiency":[327],"future.":[330]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":4},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":2}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2019-06-07T00:00:00"}