{"id":"https://openalex.org/W2946476374","doi":"https://doi.org/10.1002/dvg.23305","title":"A Chrnb3\u2010Cre BAC transgenic mouse line for manipulation of gene expression in retinal ganglion cells","display_name":"A Chrnb3\u2010Cre BAC transgenic mouse line for manipulation of gene expression in retinal ganglion cells","publication_year":2019,"publication_date":"2019-05-14","ids":{"openalex":"https://openalex.org/W2946476374","doi":"https://doi.org/10.1002/dvg.23305","mag":"2946476374","pmid":"https://pubmed.ncbi.nlm.nih.gov/31087513","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6744305"},"language":"en","primary_location":{"id":"doi:10.1002/dvg.23305","doi":"https://doi.org/10.1002/dvg.23305","is_oa":false,"landing_page_url":"https://doi.org/10.1002/dvg.23305","pdf_url":null,"source":{"id":"https://openalex.org/S124648948","display_name":"genesis","issn_l":"1526-954X","issn":["1526-954X","1526-968X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"genesis","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6744305","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042644597","display_name":"Lucy E. Drayson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1336742384","display_name":"Children's National","ror":"https://ror.org/03wa2q724","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1336742384"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lucy E. Drayson","raw_affiliation_strings":["Center for Neuroscience Research, Children's National Medical Center, Washington, DC"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Neuroscience Research, Children's National Medical Center, Washington, DC","institution_ids":["https://openalex.org/I1336742384"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5015596099","display_name":"Jason W. Triplett","orcid":"https://orcid.org/0000-0002-8064-3617","observed_orcids":["https://orcid.org/0000-0002-8064-3617"]},"institutions":[{"id":"https://openalex.org/I1336742384","display_name":"Children's National","ror":"https://ror.org/03wa2q724","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1336742384"]},{"id":"https://openalex.org/I193531525","display_name":"George Washington University","ror":"https://ror.org/00y4zzh67","country_code":"US","type":"education","lineage":["https://openalex.org/I193531525"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jason W. Triplett","raw_affiliation_strings":["Center for Neuroscience Research, Children's National Medical Center, Washington, DC","Depts. of Pediatrics and Pharmacology & Physiology The George Washington University School of Medicine and Health Sciences Washington DC"],"raw_orcid":"https://orcid.org/0000-0002-8064-3617","affiliations":[{"raw_affiliation_string":"Center for Neuroscience Research, Children's National Medical Center, Washington, DC","institution_ids":["https://openalex.org/I1336742384"]},{"raw_affiliation_string":"Depts. of Pediatrics and Pharmacology & Physiology The George Washington University School of Medicine and Health Sciences Washington DC","institution_ids":["https://openalex.org/I193531525"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5015596099"],"corresponding_institution_ids":["https://openalex.org/I1336742384","https://openalex.org/I193531525"],"apc_list":{"value":3300,"currency":"USD","value_usd":3300},"apc_paid":null,"fwci":0.0827,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.37716116,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"57","issue":"9","first_page":"e23305","last_page":"e23305"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.5443999767303467,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.5443999767303467,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12061","display_name":"Axon Guidance and Neuronal Signaling","score":0.43380001187324524,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.004699999932199717,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/retinal-ganglion-cells","display_name":"retinal ganglion cells","score":0.9380000233650208},{"id":"https://openalex.org/keywords/cre-recombinase","display_name":"Cre recombinase","score":0.6240000128746033},{"id":"https://openalex.org/keywords/cell-type-specific-gene-expression","display_name":"cell type-specific gene expression","score":0.4569999873638153}],"concepts":[{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.690168023109436},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6148642897605896},{"id":"https://openalex.org/C141035611","wikidata":"https://www.wikidata.org/wiki/Q5532934","display_name":"Genetically modified mouse","level":4,"score":0.572113573551178},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5525635480880737},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5062121748924255},{"id":"https://openalex.org/C2781334511","wikidata":"https://www.wikidata.org/wiki/Q108158","display_name":"Ganglion","level":2,"score":0.4944725036621094},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.4904308021068573},{"id":"https://openalex.org/C75323523","wikidata":"https://www.wikidata.org/wiki/Q5558489","display_name":"Giant retinal ganglion cells","level":4,"score":0.4596220552921295},{"id":"https://openalex.org/C2780827179","wikidata":"https://www.wikidata.org/wiki/Q422001","display_name":"Retinal","level":2,"score":0.4558878540992737},{"id":"https://openalex.org/C2777093970","wikidata":"https://www.wikidata.org/wiki/Q169342","display_name":"Retina","level":2,"score":0.4164056181907654},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4041720926761627},{"id":"https://openalex.org/C2777624874","wikidata":"https://www.wikidata.org/wiki/Q927337","display_name":"Retinal ganglion cell","level":3,"score":0.3252488374710083},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2903178632259369},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.22625994682312012},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.12601998448371887},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.06503164768218994}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008822","descriptor_name":"Mice, Transgenic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011978","descriptor_name":"Receptors, Nicotinic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012165","descriptor_name":"Retinal Ganglion Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D022202","descriptor_name":"Chromosomes, Artificial, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1002/dvg.23305","doi":"https://doi.org/10.1002/dvg.23305","is_oa":false,"landing_page_url":"https://doi.org/10.1002/dvg.23305","pdf_url":null,"source":{"id":"https://openalex.org/S124648948","display_name":"genesis","issn_l":"1526-954X","issn":["1526-954X","1526-968X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"genesis","raw_type":"journal-article"},{"id":"pmid:31087513","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31087513","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genesis (New York, N.Y. : 2000)","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6744305","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6744305","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Genesis","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:6744305","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6744305","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Genesis","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3928358225","display_name":"District of Columbia Intellectual and Developmental Disabilities Research Center","funder_award_id":"U54HD090257","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"},{"id":"https://openalex.org/G9000896942","display_name":"Mechanisms of Synaptic Specificity in Visual Circuits","funder_award_id":"R01EY025627","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337350","display_name":"National Eye Institute","ror":"https://ror.org/03wkg3b53"},{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W1768274470","https://openalex.org/W1974944966","https://openalex.org/W1977658700","https://openalex.org/W1993164716","https://openalex.org/W2026358414","https://openalex.org/W2079346791","https://openalex.org/W2087898838","https://openalex.org/W2104135721","https://openalex.org/W2108934548","https://openalex.org/W2134321528","https://openalex.org/W2149208048","https://openalex.org/W2162487965","https://openalex.org/W2516546718","https://openalex.org/W2770690804","https://openalex.org/W2810788577"],"related_works":["https://openalex.org/W15225339","https://openalex.org/W2589204293","https://openalex.org/W3095693204","https://openalex.org/W2074147019","https://openalex.org/W1964709109","https://openalex.org/W2050606582","https://openalex.org/W2026358414","https://openalex.org/W2030648800","https://openalex.org/W4293104994","https://openalex.org/W3199517228"],"abstract_inverted_index":{"The":[0],"mechanisms":[1],"by":[2,97],"which":[3],"retinal":[4],"ganglion":[5],"cells":[6],"(RGCs)":[7],"make":[8],"specific":[9,39],"connections":[10],"during":[11,56],"development":[12],"is":[13,113,146],"an":[14],"intense":[15],"area":[16],"of":[17,30,49,53,80,85,99,137,177,186],"research":[18],"and":[19,120,143,167],"have":[20,64],"served":[21],"as":[22],"a":[23,91,134],"model":[24],"for":[25,38],"understanding":[26,48],"the":[27,50,86,100,118,124,141,183],"general":[28],"principles":[29],"circuit":[31,190],"wiring.":[32],"As":[33],"such,":[34],"genetic":[35,173],"tools":[36],"allowing":[37],"recombination":[40],"in":[41,71,82,117,123],"RGCs":[42,116,138,158],"are":[43],"critical":[44],"to":[45,67,115,180,188],"further":[46],"our":[47],"cell-specific":[51],"roles":[52],"different":[54],"genes":[55,187],"these":[57],"processes.":[58],"However,":[59],"many":[60],"RGC-specific":[61,184],"Cre":[62,92,111,144],"lines":[63],"drawbacks,":[65],"due":[66],"their":[68],"broad":[69,83,178],"expression":[70,81,112],"other":[72],"cell":[73],"types":[74],"and/or":[75],"retinorecipient":[76,128,161],"regions":[77],"or":[78],"lack":[79],"swaths":[84],"retina.":[87],"Here,":[88],"we":[89,154],"characterize":[90],"BAC":[93],"transgenic":[94],"line":[95],"driven":[96],"elements":[98],"cholinergic":[101],"receptor":[102],"nicotinic":[103],"beta":[104],"3":[105],"subunit":[106],"(Chrnb3).":[107],"We":[108],"show":[109],"that":[110,156,163],"restricted":[114],"retina":[119,142],"sparsely":[121],"expressed":[122],"brain,":[125],"importantly":[126],"excluding":[127],"regions.":[129],"Furthermore,":[130],"Chrnb3-Cre":[131],"mice":[132],"label":[133],"wide":[135],"variety":[136],"distributed":[139],"throughout":[140],"activity":[145],"detected":[147],"embryonically,":[148],"shortly":[149],"following":[150],"RGC":[151],"differentiation.":[152],"Finally,":[153],"find":[155],"Chrnb3-Cre-labeled":[157],"innervate":[159],"multiple":[160],"areas":[162],"serve":[164],"both":[165],"image-forming":[166],"nonimage":[168],"forming":[169],"functions.":[170],"Thus,":[171],"this":[172],"tool":[174],"will":[175],"be":[176],"use":[179],"investigators":[181],"studying":[182],"contributions":[185],"visual":[189],"development.":[191]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2021,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2019-05-29T00:00:00"}