{"id":"https://openalex.org/W2945507494","doi":"https://doi.org/10.3791/59294","title":"Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes","display_name":"Hybrid Clear/Blue Native Electrophoresis for the Separation and Analysis of Mitochondrial Respiratory Chain Supercomplexes","publication_year":2019,"publication_date":"2019-05-19","ids":{"openalex":"https://openalex.org/W2945507494","doi":"https://doi.org/10.3791/59294","mag":"2945507494","pmid":"https://pubmed.ncbi.nlm.nih.gov/31157764"},"language":"en","primary_location":{"id":"doi:10.3791/59294","doi":"https://doi.org/10.3791/59294","is_oa":true,"landing_page_url":"https://doi.org/10.3791/59294","pdf_url":"https://www.jove.com/pdf/59294/hybrid-clearblue-native-electrophoresis-for-separation-analysis","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.jove.com/pdf/59294/hybrid-clearblue-native-electrophoresis-for-separation-analysis","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5036713348","display_name":"Alexanne Cuillerier","orcid":"https://orcid.org/0000-0001-8505-3758","observed_orcids":["https://orcid.org/0000-0001-8505-3758"]},"institutions":[{"id":"https://openalex.org/I153718931","display_name":"University of Ottawa","ror":"https://ror.org/03c4mmv16","country_code":"CA","type":"education","lineage":["https://openalex.org/I153718931"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Alexanne Cuillerier","raw_affiliation_strings":["Interdisciplinary School of Health Science, Faculty of Health Sciences and Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interdisciplinary School of Health Science, Faculty of Health Sciences and Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa","institution_ids":["https://openalex.org/I153718931"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003591293","display_name":"Yan Burelle","orcid":"https://orcid.org/0000-0001-9379-146X","observed_orcids":["https://orcid.org/0000-0001-9379-146X"]},"institutions":[{"id":"https://openalex.org/I153718931","display_name":"University of Ottawa","ror":"https://ror.org/03c4mmv16","country_code":"CA","type":"education","lineage":["https://openalex.org/I153718931"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Yan Burelle","raw_affiliation_strings":["Interdisciplinary School of Health Science, Faculty of Health Sciences and Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa; yburell2@uottawa.ca","Interdisciplinary School of Health Science, Faculty of Health Sciences and Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interdisciplinary School of Health Science, Faculty of Health Sciences and Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa; yburell2@uottawa.ca","institution_ids":["https://openalex.org/I153718931"]},{"raw_affiliation_string":"Interdisciplinary School of Health Science, Faculty of Health Sciences and Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa","institution_ids":["https://openalex.org/I153718931"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I153718931"],"apc_list":null,"apc_paid":null,"fwci":0.291,"has_fulltext":true,"cited_by_count":8,"citation_normalized_percentile":{"value":0.46083114,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":null,"issue":"147","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.9345999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10301","display_name":"Mitochondrial Function and Pathology","score":0.9345999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.032099999487400055,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12763","display_name":"ATP Synthase and ATPases Research","score":0.006899999920278788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/blue-native-page","display_name":"blue native PAGE","score":0.6570000052452087},{"id":"https://openalex.org/keywords/coomassie-brilliant-blue-staining","display_name":"Coomassie blue staining","score":0.8870000243186951},{"id":"https://openalex.org/keywords/digitonin-extraction","display_name":"digitonin extraction","score":0.8970000147819519},{"id":"https://openalex.org/keywords/oxidative-phosphorylation","display_name":"oxidative phosphorylation","score":0.8700000047683716},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.781000018119812},{"id":"https://openalex.org/keywords/mitochondrial-proteomics","display_name":"mitochondrial proteomics","score":0.597000002861023}],"concepts":[{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.5974665880203247},{"id":"https://openalex.org/C28859421","wikidata":"https://www.wikidata.org/wiki/Q39572","display_name":"Mitochondrion","level":2,"score":0.5135407447814941},{"id":"https://openalex.org/C40250595","wikidata":"https://www.wikidata.org/wiki/Q185098","display_name":"Electrophoresis","level":2,"score":0.5114971399307251},{"id":"https://openalex.org/C57600042","wikidata":"https://www.wikidata.org/wiki/Q227564","display_name":"Oxidative phosphorylation","level":2,"score":0.4933001399040222},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.48923349380493164},{"id":"https://openalex.org/C2780761556","wikidata":"https://www.wikidata.org/wiki/Q418516","display_name":"Coomassie Brilliant Blue","level":3,"score":0.4747515022754669},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.46047985553741455},{"id":"https://openalex.org/C2778920010","wikidata":"https://www.wikidata.org/wiki/Q410968","display_name":"Digitonin","level":3,"score":0.4481087923049927},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4353148937225342},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4236214756965637},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42282718420028687},{"id":"https://openalex.org/C3020377403","wikidata":"https://www.wikidata.org/wiki/Q211016","display_name":"Respiratory chain","level":3,"score":0.4218995273113251},{"id":"https://openalex.org/C3020761558","wikidata":"https://www.wikidata.org/wiki/Q211016","display_name":"Mitochondrial respiratory chain","level":3,"score":0.41820722818374634},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3467344045639038},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.34320545196533203},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2215820550918579},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1781441867351532},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.08380380272865295},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.08375754952430725}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004072","descriptor_name":"Digitonin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004579","descriptor_name":"Electron Transport","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008930","descriptor_name":"Mitochondria, Liver","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010085","descriptor_name":"Oxidative Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D024101","descriptor_name":"Mitochondrial Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D045222","descriptor_name":"Electron Transport Chain Complex Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D063347","descriptor_name":"Native Polyacrylamide Gel Electrophoresis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.3791/59294","doi":"https://doi.org/10.3791/59294","is_oa":true,"landing_page_url":"https://doi.org/10.3791/59294","pdf_url":"https://www.jove.com/pdf/59294/hybrid-clearblue-native-electrophoresis-for-separation-analysis","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"doi:10.3791/59294-v","doi":"https://doi.org/10.3791/59294-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/59294-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmid:31157764","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31157764","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.3791/59294","doi":"https://doi.org/10.3791/59294","is_oa":true,"landing_page_url":"https://doi.org/10.3791/59294","pdf_url":"https://www.jove.com/pdf/59294/hybrid-clearblue-native-electrophoresis-for-separation-analysis","source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334506","display_name":"Canadian Institutes of Health Research","ror":"https://ror.org/01gavpb45"},{"id":"https://openalex.org/F4320334593","display_name":"Natural Sciences and Engineering Research Council of Canada","ror":"https://ror.org/01h531d29"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2945507494.pdf","grobid_xml":"https://content.openalex.org/works/W2945507494.grobid-xml"},"referenced_works_count":34,"referenced_works":["https://openalex.org/W114739423","https://openalex.org/W1414125020","https://openalex.org/W1541314725","https://openalex.org/W1595641366","https://openalex.org/W1965285497","https://openalex.org/W1984019957","https://openalex.org/W1986248905","https://openalex.org/W2000513681","https://openalex.org/W2021129026","https://openalex.org/W2031972450","https://openalex.org/W2037775127","https://openalex.org/W2041152525","https://openalex.org/W2052165629","https://openalex.org/W2062821451","https://openalex.org/W2068119233","https://openalex.org/W2086483420","https://openalex.org/W2090734611","https://openalex.org/W2094999489","https://openalex.org/W2115528784","https://openalex.org/W2116444561","https://openalex.org/W2131226021","https://openalex.org/W2145118242","https://openalex.org/W2153821641","https://openalex.org/W2258075909","https://openalex.org/W2305670323","https://openalex.org/W2467200423","https://openalex.org/W2556127230","https://openalex.org/W2558143992","https://openalex.org/W2604606314","https://openalex.org/W2617533299","https://openalex.org/W2620759218","https://openalex.org/W2790631558","https://openalex.org/W2805266700","https://openalex.org/W4211246989"],"related_works":["https://openalex.org/W2305670323","https://openalex.org/W3164782150","https://openalex.org/W3083018475","https://openalex.org/W4412802290","https://openalex.org/W4300547233","https://openalex.org/W2620759218","https://openalex.org/W4396640711","https://openalex.org/W7135781652","https://openalex.org/W4210718406","https://openalex.org/W2467272275"],"abstract_inverted_index":{"Complexes":[0],"of":[1,39,43,58,80,106,124,155,197],"the":[2,78,107,115,122,125,152],"oxidative":[3],"phosphorylation":[4],"machinery":[5],"form":[6],"supramolecular":[7],"protein":[8],"arrangements":[9],"named":[10],"supercomplexes":[11],"(SCs),":[12],"which":[13,65],"are":[14,60,111],"believed":[15],"to":[16,22,33,86,114,129,135,138,176],"confer":[17],"structural":[18],"and":[19,35,48,82,88,140,163,179],"functional":[20],"advantages":[21,79],"mitochondria.":[23],"SCs":[24,90,101,142],"have":[25],"been":[26],"identified":[27],"in":[28,46,62,91,181],"many":[29],"species,":[30],"from":[31],"yeast":[32],"mammal,":[34],"an":[36,55,73],"increasing":[37,56],"number":[38,57],"studies":[40],"report":[41],"disruption":[42],"their":[44],"organization":[45],"genetic":[47],"acquired":[49],"human":[50],"diseases.":[51],"As":[52],"a":[53,92],"result,":[54],"laboratories":[59],"interested":[61],"analyzing":[63],"SCs,":[64],"can":[66],"be":[67],"methodologically":[68],"challenging.":[69],"This":[70,132],"article":[71],"presents":[72],"optimized":[74],"protocol":[75,171],"that":[76],"combines":[77],"Blue-":[81],"Clear-Native":[83],"PAGE":[84],"methods":[85],"resolve":[87,141],"analyze":[89],"time-effective":[93],"manner.":[94],"With":[95,169],"this":[96,170],"hybrid":[97],"CN/BN-PAGE":[98],"method,":[99],"mitochondrial":[100],"extracted":[102],"with":[103,146,185],"optimal":[104],"amounts":[105],"mild":[108],"detergent":[109],"digitonin":[110],"exposed":[112],"briefly":[113],"anionic":[116],"dye":[117],"Coomassie":[118],"Blue":[119],"(CB)":[120],"at":[121],"beginning":[123],"electrophoresis,":[126,190],"without":[127],"exposure":[128,134],"other":[130],"detergents.":[131],"short":[133],"CB":[136,157],"allows":[137],"separate":[139],"as":[143,145],"effectively":[144],"traditional":[147],"BN-PAGE":[148],"methods,":[149],"while":[150],"avoiding":[151],"negative":[153],"impact":[154],"high":[156],"levels":[158],"on":[159],"in-gel":[160],"activity":[161,183],"assays,":[162],"labile":[164],"protein-protein":[165],"interactions":[166],"within":[167],"SCs.":[168,198],"it":[172],"is":[173],"thus":[174],"possible":[175],"combine":[177],"precise":[178],"rapid":[180],"gel":[182],"measurements":[184],"analytical":[186],"techniques":[187],"involving":[188],"2D":[189],"immuno-detection,":[191],"and/or":[192],"proteomics":[193],"for":[194],"advanced":[195],"analysis":[196]},"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2019-05-29T00:00:00"}