{"id":"https://openalex.org/W2943932287","doi":"https://doi.org/10.7554/elife.46135","title":"Generation of endogenous pH-sensitive EGF receptor and its application in high-throughput screening for proteins involved in clathrin-mediated endocytosis","display_name":"Generation of endogenous pH-sensitive EGF receptor and its application in high-throughput screening for proteins involved in clathrin-mediated endocytosis","publication_year":2019,"publication_date":"2019-05-08","ids":{"openalex":"https://openalex.org/W2943932287","doi":"https://doi.org/10.7554/elife.46135","mag":"2943932287","pmid":"https://pubmed.ncbi.nlm.nih.gov/31066673","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6533059"},"language":"en","primary_location":{"id":"doi:10.7554/elife.46135","doi":"https://doi.org/10.7554/elife.46135","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.46135","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"eLife","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.7554/elife.46135","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5076006747","display_name":"Mads Breum Larsen","orcid":"https://orcid.org/0000-0001-5361-0898","observed_orcids":["https://orcid.org/0000-0001-5361-0898"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mads Breum Larsen","raw_affiliation_strings":["Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, United States","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003668173","display_name":"Mireia P\u00e9rez-Verdaguer","orcid":"https://orcid.org/0000-0003-0875-8062","observed_orcids":["https://orcid.org/0000-0003-0875-8062"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mireia Perez Verdaguer","raw_affiliation_strings":["Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, United States","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046544591","display_name":"Brigitte F. Schmidt","orcid":"https://orcid.org/0000-0002-2892-493X","observed_orcids":["https://orcid.org/0000-0002-2892-493X"]},"institutions":[{"id":"https://openalex.org/I74973139","display_name":"Carnegie Mellon University","ror":"https://ror.org/05x2bcf33","country_code":"US","type":"education","lineage":["https://openalex.org/I74973139"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brigitte F Schmidt","raw_affiliation_strings":["Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, United States","institution_ids":["https://openalex.org/I74973139"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028325980","display_name":"Marcel P. Bruchez","orcid":"https://orcid.org/0000-0002-7370-4848","observed_orcids":["https://orcid.org/0000-0002-7370-4848"]},"institutions":[{"id":"https://openalex.org/I4210092523","display_name":"Sharp Edge Labs (United States)","ror":"https://ror.org/00m92qj63","country_code":"US","type":"company","lineage":["https://openalex.org/I4210092523"]},{"id":"https://openalex.org/I74973139","display_name":"Carnegie Mellon University","ror":"https://ror.org/05x2bcf33","country_code":"US","type":"education","lineage":["https://openalex.org/I74973139"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marcel P Bruchez","raw_affiliation_strings":["Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, United States","Department of Chemistry, Carnegie Mellon University, Pittsburgh, United States","Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, United States","Sharp Edge Laboratories, Pittsburgh, United States"],"raw_orcid":"https://orcid.org/0000-0002-7370-4848","affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, United States","institution_ids":["https://openalex.org/I74973139"]},{"raw_affiliation_string":"Department of Chemistry, Carnegie Mellon University, Pittsburgh, United States","institution_ids":["https://openalex.org/I74973139"]},{"raw_affiliation_string":"Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, United States","institution_ids":["https://openalex.org/I74973139"]},{"raw_affiliation_string":"Sharp Edge Laboratories, Pittsburgh, United States","institution_ids":["https://openalex.org/I4210092523"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088924932","display_name":"Simon C. Watkins","orcid":"https://orcid.org/0000-0003-4092-1552","observed_orcids":["https://orcid.org/0000-0003-4092-1552","https://orcid.org/0000-0003-0825-0795"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Simon C Watkins","raw_affiliation_strings":["Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, United States"],"raw_orcid":"https://orcid.org/0000-0003-4092-1552","affiliations":[{"raw_affiliation_string":"Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, United States","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5073726679","display_name":"Alexander D. Sorkin","orcid":"https://orcid.org/0000-0002-4446-1920","observed_orcids":["https://orcid.org/0000-0002-4446-1920"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Alexander Sorkin","raw_affiliation_strings":["Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, United States"],"raw_orcid":"https://orcid.org/0000-0002-4446-1920","affiliations":[{"raw_affiliation_string":"Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, United States","institution_ids":["https://openalex.org/I170201317"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5073726679","https://openalex.org/A5088924932"],"corresponding_institution_ids":["https://openalex.org/I170201317"],"apc_list":{"value":3000,"currency":"USD","value_usd":2500},"apc_paid":{"value":3000,"currency":"USD","value_usd":2500},"fwci":0.794,"has_fulltext":false,"cited_by_count":9,"citation_normalized_percentile":{"value":0.65786299,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"8","issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.001500000013038516,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/egf-receptor","display_name":"EGF receptor","score":0.9089999794960022},{"id":"https://openalex.org/keywords/clathrin-mediated-endocytosis","display_name":"clathrin-mediated endocytosis","score":0.9169999957084656},{"id":"https://openalex.org/keywords/fluorogen-activating-protein","display_name":"fluorogen-activating protein","score":0.9800000190734863},{"id":"https://openalex.org/keywords/ratiometric-fluorescence","display_name":"ratiometric fluorescence","score":0.6600000262260437},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.7260000109672546},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.8190000057220459},{"id":"https://openalex.org/keywords/receptor-internalization","display_name":"receptor internalization","score":0.5410000085830688}],"concepts":[{"id":"https://openalex.org/C28005876","wikidata":"https://www.wikidata.org/wiki/Q189814","display_name":"Endocytosis","level":3,"score":0.8905824422836304},{"id":"https://openalex.org/C117943116","wikidata":"https://www.wikidata.org/wiki/Q1135604","display_name":"Receptor-mediated endocytosis","level":4,"score":0.6287972331047058},{"id":"https://openalex.org/C102747710","wikidata":"https://www.wikidata.org/wiki/Q824237","display_name":"Endosome","level":3,"score":0.6211861371994019},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5899127125740051},{"id":"https://openalex.org/C63162447","wikidata":"https://www.wikidata.org/wiki/Q21100804","display_name":"Clathrin","level":4,"score":0.4897770881652832},{"id":"https://openalex.org/C2779438470","wikidata":"https://www.wikidata.org/wiki/Q424401","display_name":"Epidermal growth factor receptor","level":3,"score":0.4689438045024872},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.446618914604187},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41610345244407654},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.40212520956993103},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.36339226365089417},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.30628830194473267},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.26344430446624756}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002966","descriptor_name":"Clathrin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004705","descriptor_name":"Endocytosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013057","descriptor_name":"Spectrum Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066246","descriptor_name":"ErbB Receptors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D066246","descriptor_name":"ErbB Receptors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.7554/elife.46135","doi":"https://doi.org/10.7554/elife.46135","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.46135","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"eLife","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6533059","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6533059","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"eLife","raw_type":"Text"},{"id":"pmid:31066673","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31066673","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"eLife","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:ff22768ff9c84a719bbb4e8178a8f07b","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/ff22768ff9c84a719bbb4e8178a8f07b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"eLife, Vol 8 (2019)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.7554/elife.46135","doi":"https://doi.org/10.7554/elife.46135","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.46135","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"eLife","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2600100738","display_name":"Signaling by the EGF Receptor from Endosomes","funder_award_id":"R01GM124186","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G3258829441","display_name":"Targeted fluorescent indicators for endothelial physiology: Ca(II), ROS, NO","funder_award_id":"R01EB017268","funder_id":"https://openalex.org/F4320337363","funder_display_name":"National Institute of Biomedical Imaging and Bioengineering"},{"id":"https://openalex.org/G8131959350","display_name":"Fluorogen Activating Peptide-Based FRET to Quantify FceRI Activation Mechanisms","funder_award_id":"R01GM114075","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G8268723693","display_name":"Pathogenesis of Cancer - Role of EGF Receptor Endocytosis","funder_award_id":"R01CA089151","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337363","display_name":"National Institute of Biomedical Imaging and Bioengineering","ror":"https://ror.org/00372qc85"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":59,"referenced_works":["https://openalex.org/W73139964","https://openalex.org/W1565471250","https://openalex.org/W1585913386","https://openalex.org/W1896889801","https://openalex.org/W1966311581","https://openalex.org/W1967626450","https://openalex.org/W1972960760","https://openalex.org/W1973491865","https://openalex.org/W1988193314","https://openalex.org/W1991001144","https://openalex.org/W1993355802","https://openalex.org/W1999514574","https://openalex.org/W1999769461","https://openalex.org/W2017216400","https://openalex.org/W2017923651","https://openalex.org/W2018492687","https://openalex.org/W2019772485","https://openalex.org/W2028415754","https://openalex.org/W2029287429","https://openalex.org/W2032444338","https://openalex.org/W2037383809","https://openalex.org/W2044075747","https://openalex.org/W2061951348","https://openalex.org/W2064315321","https://openalex.org/W2065221771","https://openalex.org/W2068807411","https://openalex.org/W2069981183","https://openalex.org/W2072523592","https://openalex.org/W2086515708","https://openalex.org/W2094009861","https://openalex.org/W2096483233","https://openalex.org/W2100272194","https://openalex.org/W2102580914","https://openalex.org/W2111625999","https://openalex.org/W2112308680","https://openalex.org/W2118618143","https://openalex.org/W2125251546","https://openalex.org/W2128574297","https://openalex.org/W2133809169","https://openalex.org/W2143891754","https://openalex.org/W2145460819","https://openalex.org/W2149766306","https://openalex.org/W2170019668","https://openalex.org/W2231430019","https://openalex.org/W2317871805","https://openalex.org/W2570114214","https://openalex.org/W2576331818","https://openalex.org/W2584952838","https://openalex.org/W2590493137","https://openalex.org/W2612421949","https://openalex.org/W2627939525","https://openalex.org/W2747121213","https://openalex.org/W2755763754","https://openalex.org/W2766624035","https://openalex.org/W2767430428","https://openalex.org/W2769423727","https://openalex.org/W2787464998","https://openalex.org/W2890315538","https://openalex.org/W2903644795"],"related_works":["https://openalex.org/W2994830059","https://openalex.org/W2032504743","https://openalex.org/W1993355802","https://openalex.org/W2050228149","https://openalex.org/W3164714602","https://openalex.org/W4224436820","https://openalex.org/W2904616827","https://openalex.org/W2062069749","https://openalex.org/W2138641998","https://openalex.org/W2123235908"],"abstract_inverted_index":{"Previously":[0],"we":[1],"used":[2],"gene-editing":[3,37],"to":[4,38,57,81,109,126],"label":[5],"endogenous":[6,138],"EGF":[7,120],"receptor":[8],"(EGFR)":[9],"with":[10,64,97,118],"GFP":[11],"and":[12,22,68],"demonstrate":[13],"that":[14],"picomolar":[15],"concentrations":[16],"of":[17,24,51,76,86,105,113,137,145],"EGFR":[18,25,47],"ligand":[19],"drive":[20],"signaling":[21],"endocytosis":[23,116],"in":[26,28,45,89,142],"tumors":[27],"vivo":[29],"(Pinilla-Macua":[30],"et":[31],"al.,":[32],"2017).":[33],"We":[34],"now":[35],"use":[36],"insert":[39],"a":[40,60,69,106,128],"fluorogen":[41],"activating":[42],"protein":[43],"(FAP)":[44],"the":[46,52,84,98,111,135],"extracellular":[48],"domain.":[49],"Binding":[50],"tandem":[53],"dye":[54],"pair":[55],"MG-Bis-SA":[56],"FAP-EGFR":[58,87,95,115],"provides":[59],"ratiometric":[61],"pH-sensitive":[62,139],"model":[63],"dual":[65],"fluorescence":[66,77],"excitation":[67,74],"single":[70],"far-red":[71],"emission.":[72],"The":[73,122],"ratio":[75],"intensities":[78],"was":[79,124],"demonstrated":[80],"faithfully":[82],"report":[83],"fraction":[85],"located":[88],"acidic":[90],"endosomal/lysosomal":[91],"compartments.":[92],"Coupling":[93],"native":[94],"expression":[96],"high":[99],"method":[100],"sensitivity":[101],"has":[102],"allowed":[103],"development":[104],"high-throughput":[107,143],"assay":[108,123],"measure":[110],"rates":[112],"clathrin-mediated":[114],"stimulated":[117],"physiological":[119],"concentrations.":[121],"utilized":[125],"screen":[127],"phosphatase":[129],"siRNA":[130],"library.":[131],"These":[132],"studies":[133],"highlight":[134],"utility":[136],"FAP-receptor":[140],"chimeras":[141],"analysis":[144],"endocytosis.":[146]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2019-05-16T00:00:00"}