{"id":"https://openalex.org/W2942874686","doi":"https://doi.org/10.1016/j.vaccine.2019.04.054","title":"Semi-perfusion cultures of suspension MDCK cells enable high cell concentrations and efficient influenza A virus production","display_name":"Semi-perfusion cultures of suspension MDCK cells enable high cell concentrations and efficient influenza A virus production","publication_year":2019,"publication_date":"2019-04-29","ids":{"openalex":"https://openalex.org/W2942874686","doi":"https://doi.org/10.1016/j.vaccine.2019.04.054","mag":"2942874686","pmid":"https://pubmed.ncbi.nlm.nih.gov/31047676"},"language":"en","primary_location":{"id":"doi:10.1016/j.vaccine.2019.04.054","doi":"https://doi.org/10.1016/j.vaccine.2019.04.054","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.vaccine.2019.04.054","pdf_url":"https://www.sciencedirect.com/science/article/pii/S0264410X19305286/pdf","source":{"id":"https://openalex.org/S27359860","display_name":"Vaccine","issn_l":"0264-410X","issn":["0264-410X","1873-2518"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Vaccine","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://www.sciencedirect.com/science/article/pii/S0264410X19305286/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042655293","display_name":"Thomas Bissinger","orcid":"https://orcid.org/0000-0002-6901-6161","observed_orcids":["https://orcid.org/0000-0002-6901-6161","https://orcid.org/0000-0002-0775-3860"]},"institutions":[{"id":"https://openalex.org/I4210127568","display_name":"Max Planck Institute for Dynamics of Complex Technical Systems","ror":"https://ror.org/030h7k016","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210127568"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Thomas Bissinger","raw_affiliation_strings":["Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany. Electronic address: bissinger@mpi-magdeburg.mpg.de","Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany. Electronic address: bissinger@mpi-magdeburg.mpg.de","institution_ids":["https://openalex.org/I4210127568"]},{"raw_affiliation_string":"Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany","institution_ids":["https://openalex.org/I4210127568"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113910886","display_name":"Johannes Fritsch","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210127568","display_name":"Max Planck Institute for Dynamics of Complex Technical Systems","ror":"https://ror.org/030h7k016","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210127568"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Johannes Fritsch","raw_affiliation_strings":["Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany","institution_ids":["https://openalex.org/I4210127568"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5097787117","display_name":"Adrian Mihut","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210127568","display_name":"Max Planck Institute for Dynamics of Complex Technical Systems","ror":"https://ror.org/030h7k016","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210127568"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Adrian Mihut","raw_affiliation_strings":["Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany","institution_ids":["https://openalex.org/I4210127568"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053737217","display_name":"Yixiao Wu","orcid":"https://orcid.org/0000-0002-3616-3026","observed_orcids":["https://orcid.org/0000-0002-3616-3026"]},"institutions":[{"id":"https://openalex.org/I143593769","display_name":"East China University of Science and Technology","ror":"https://ror.org/01vyrm377","country_code":"CN","type":"education","lineage":["https://openalex.org/I143593769"]},{"id":"https://openalex.org/I4391768089","display_name":"State Key Laboratory of Bioreactor Engineering","ror":"https://ror.org/02hqczw19","country_code":"CN","type":"facility","lineage":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yixiao Wu","raw_affiliation_strings":["State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, China","institution_ids":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064571951","display_name":"Xuping Liu","orcid":"https://orcid.org/0000-0001-5032-1185","observed_orcids":["https://orcid.org/0000-0001-5032-1185"]},"institutions":[{"id":"https://openalex.org/I143593769","display_name":"East China University of Science and Technology","ror":"https://ror.org/01vyrm377","country_code":"CN","type":"education","lineage":["https://openalex.org/I143593769"]},{"id":"https://openalex.org/I4391768089","display_name":"State Key Laboratory of Bioreactor Engineering","ror":"https://ror.org/02hqczw19","country_code":"CN","type":"facility","lineage":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xuping Liu","raw_affiliation_strings":["State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, China","institution_ids":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084150472","display_name":"Yvonne Genzel","orcid":"https://orcid.org/0000-0002-2652-5943","observed_orcids":["https://orcid.org/0000-0002-2652-5943"]},"institutions":[{"id":"https://openalex.org/I4210127568","display_name":"Max Planck Institute for Dynamics of Complex Technical Systems","ror":"https://ror.org/030h7k016","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210127568"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Yvonne Genzel","raw_affiliation_strings":["Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany"],"raw_orcid":"https://orcid.org/0000-0002-2652-5943","affiliations":[{"raw_affiliation_string":"Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany","institution_ids":["https://openalex.org/I4210127568"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033997863","display_name":"Wen\u2010Song Tan","orcid":"https://orcid.org/0000-0001-9857-4798","observed_orcids":["https://orcid.org/0000-0001-9857-4798"]},"institutions":[{"id":"https://openalex.org/I143593769","display_name":"East China University of Science and Technology","ror":"https://ror.org/01vyrm377","country_code":"CN","type":"education","lineage":["https://openalex.org/I143593769"]},{"id":"https://openalex.org/I4391768089","display_name":"State Key Laboratory of Bioreactor Engineering","ror":"https://ror.org/02hqczw19","country_code":"CN","type":"facility","lineage":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wen-Song Tan","raw_affiliation_strings":["State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, China","institution_ids":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5082695733","display_name":"Udo Reichl","orcid":"https://orcid.org/0000-0001-6538-1332","observed_orcids":["https://orcid.org/0000-0001-6538-1332"]},"institutions":[{"id":"https://openalex.org/I4210127568","display_name":"Max Planck Institute for Dynamics of Complex Technical Systems","ror":"https://ror.org/030h7k016","country_code":"DE","type":"facility","lineage":["https://openalex.org/I149899117","https://openalex.org/I4210127568"]},{"id":"https://openalex.org/I95793202","display_name":"Otto-von-Guericke-Universit\u00e4t Magdeburg","ror":"https://ror.org/00ggpsq73","country_code":"DE","type":"education","lineage":["https://openalex.org/I95793202"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Udo Reichl","raw_affiliation_strings":["Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany; Chair of Bioprocess Engineering, Otto-von-Guericke University Magdeburg, Universitaetsplatz 2, 39106 Magdeburg, Germany","Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany","Chair of Bioprocess Engineering, Otto-von-Guericke University Magdeburg, Universitaetsplatz 2, 39106 Magdeburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany; Chair of Bioprocess Engineering, Otto-von-Guericke University Magdeburg, Universitaetsplatz 2, 39106 Magdeburg, Germany","institution_ids":["https://openalex.org/I4210127568","https://openalex.org/I95793202"]},{"raw_affiliation_string":"Bioprocess Engineering Group, Max Planck Institute for Dynamics of Complex Technical Systems, Sandtorstrasse 1, 39106 Magdeburg, Germany","institution_ids":["https://openalex.org/I4210127568"]},{"raw_affiliation_string":"Chair of Bioprocess Engineering, Otto-von-Guericke University Magdeburg, Universitaetsplatz 2, 39106 Magdeburg, Germany","institution_ids":["https://openalex.org/I95793202"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5042655293"],"corresponding_institution_ids":["https://openalex.org/I4210127568"],"apc_list":{"value":2450,"currency":"USD","value_usd":2450},"apc_paid":{"value":2450,"currency":"USD","value_usd":2450},"fwci":1.6115,"has_fulltext":true,"cited_by_count":46,"citation_normalized_percentile":{"value":0.83323738,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"37","issue":"47","first_page":"7003","last_page":"7010"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9531000256538391,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9531000256538391,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10167","display_name":"Influenza Virus Research Studies","score":0.046300001442432404,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11135","display_name":"Virology and Viral Diseases","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/influenza-a-virus","display_name":"influenza A virus","score":0.8309999704360962},{"id":"https://openalex.org/keywords/mdck-cells","display_name":"MDCK cells","score":0.7699999809265137},{"id":"https://openalex.org/keywords/cell-suspension-culture","display_name":"cell suspension culture","score":0.9020000100135803},{"id":"https://openalex.org/keywords/high-cell-density","display_name":"high cell density","score":0.7630000114440918},{"id":"https://openalex.org/keywords/inactivated-influenza-vaccine","display_name":"inactivated influenza vaccine","score":0.8240000009536743},{"id":"https://openalex.org/keywords/cell-culture-bioreactors","display_name":"cell culture bioreactors","score":0.47200000286102295},{"id":"https://openalex.org/keywords/process-intensification","display_name":"process intensification","score":0.7139999866485596}],"concepts":[{"id":"https://openalex.org/C129470801","wikidata":"https://www.wikidata.org/wiki/Q5370234","display_name":"Embryonated","level":3,"score":0.9103114604949951},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.790006160736084},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.7465105652809143},{"id":"https://openalex.org/C32611913","wikidata":"https://www.wikidata.org/wiki/Q2166189","display_name":"Titer","level":3,"score":0.7098937034606934},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.678340494632721},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5539723634719849},{"id":"https://openalex.org/C2777546802","wikidata":"https://www.wikidata.org/wiki/Q834390","display_name":"Influenza A virus","level":3,"score":0.5057899951934814},{"id":"https://openalex.org/C156725737","wikidata":"https://www.wikidata.org/wiki/Q7933598","display_name":"Viral culture","level":3,"score":0.46617698669433594},{"id":"https://openalex.org/C2780689484","wikidata":"https://www.wikidata.org/wiki/Q383260","display_name":"Influenza vaccine","level":3,"score":0.44910287857055664},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.40088382363319397},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004285","descriptor_name":"Dogs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007252","descriptor_name":"Influenza Vaccines","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D009976","descriptor_name":"Orthomyxoviridae Infections","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D009980","descriptor_name":"Influenza A virus","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":true},{"descriptor_ui":"D009980","descriptor_name":"Influenza A virus","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D014771","descriptor_name":"Virion","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D014776","descriptor_name":"Virus Cultivation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D014779","descriptor_name":"Virus Replication","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D019149","descriptor_name":"Bioreactors","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D061249","descriptor_name":"Batch Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D061985","descriptor_name":"Madin Darby Canine Kidney Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.vaccine.2019.04.054","doi":"https://doi.org/10.1016/j.vaccine.2019.04.054","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.vaccine.2019.04.054","pdf_url":"https://www.sciencedirect.com/science/article/pii/S0264410X19305286/pdf","source":{"id":"https://openalex.org/S27359860","display_name":"Vaccine","issn_l":"0264-410X","issn":["0264-410X","1873-2518"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Vaccine","raw_type":"journal-article"},{"id":"pmid:31047676","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/31047676","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Vaccine","raw_type":"Journal Article"},{"id":"pmh:oai:pure.mpg.de:item_3149974","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/21.11116/0000-0004-64C9-9","pdf_url":null,"source":{"id":"https://openalex.org/S4306400654","display_name":"MPG.PuRe (Max Planck Society)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I149899117","host_organization_name":"Max Planck Society","host_organization_lineage":["https://openalex.org/I149899117"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Vaccine","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1016/j.vaccine.2019.04.054","doi":"https://doi.org/10.1016/j.vaccine.2019.04.054","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.vaccine.2019.04.054","pdf_url":"https://www.sciencedirect.com/science/article/pii/S0264410X19305286/pdf","source":{"id":"https://openalex.org/S27359860","display_name":"Vaccine","issn_l":"0264-410X","issn":["0264-410X","1873-2518"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Vaccine","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8371999859809875}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2942874686.pdf","grobid_xml":"https://content.openalex.org/works/W2942874686.grobid-xml"},"referenced_works_count":49,"referenced_works":["https://openalex.org/W589059346","https://openalex.org/W781343893","https://openalex.org/W1595205689","https://openalex.org/W1965833109","https://openalex.org/W1968801913","https://openalex.org/W1970156677","https://openalex.org/W1972107263","https://openalex.org/W1978659446","https://openalex.org/W1987958868","https://openalex.org/W1989069389","https://openalex.org/W1992753756","https://openalex.org/W2006401683","https://openalex.org/W2009118939","https://openalex.org/W2018040189","https://openalex.org/W2026124052","https://openalex.org/W2031693543","https://openalex.org/W2039944386","https://openalex.org/W2040842681","https://openalex.org/W2043996181","https://openalex.org/W2048030544","https://openalex.org/W2048726798","https://openalex.org/W2052443261","https://openalex.org/W2053189633","https://openalex.org/W2054209117","https://openalex.org/W2055665521","https://openalex.org/W2061022369","https://openalex.org/W2078514070","https://openalex.org/W2080712703","https://openalex.org/W2084976117","https://openalex.org/W2091991657","https://openalex.org/W2094663611","https://openalex.org/W2100316471","https://openalex.org/W2106476921","https://openalex.org/W2125761044","https://openalex.org/W2134015847","https://openalex.org/W2143686508","https://openalex.org/W2154786862","https://openalex.org/W2188048318","https://openalex.org/W2235891297","https://openalex.org/W2725836970","https://openalex.org/W2739140450","https://openalex.org/W2793819443","https://openalex.org/W2800956912","https://openalex.org/W2886174163","https://openalex.org/W2889891640","https://openalex.org/W4211071649","https://openalex.org/W4233108527","https://openalex.org/W4292550103","https://openalex.org/W4322699774"],"related_works":["https://openalex.org/W3125188899","https://openalex.org/W4409729310","https://openalex.org/W4407843450","https://openalex.org/W3134316664","https://openalex.org/W800437016","https://openalex.org/W801914584","https://openalex.org/W2936246550","https://openalex.org/W1972945328","https://openalex.org/W3162448264","https://openalex.org/W1972107263"],"abstract_inverted_index":{"Control":[0],"and":[1,15,18,122,148,171],"prevention":[2],"of":[3,13,92,144,151],"rapid":[4],"influenza":[5,25,29,166],"spread":[6],"among":[7],"humans":[8],"depend":[9],"on":[10,35,40],"the":[11,176],"availability":[12],"efficient":[14],"safe":[16],"seasonal":[17],"pandemic":[19],"vaccines,":[20],"made":[21],"primarily":[22],"from":[23],"inactivated":[24],"virus":[26,30,46,110,127,142,167],"particles.":[27],"Current":[28],"production":[31],"processes":[32],"rely":[33],"heavily":[34],"embryonated":[36],"chicken":[37],"eggs":[38],"or":[39],"cell":[41,55,68,79,86,98,164],"culture":[42,56],"as":[43,84,175],"substrate":[44],"for":[45,120,125,163],"propagation.":[47],"Today\u2019s":[48],"efforts":[49],"towards":[50],"process":[51],"intensification":[52],"in":[53,66,105,113,179],"animal":[54],"could":[57],"innovate":[58],"viral":[59,180],"vaccine":[60,181],"manufacturing":[61],"using":[62],"high-yield":[63],"suspension":[64,87],"cells":[65],"high":[67],"density":[69],"perfusion":[70,173],"processes.":[71],"In":[72,131],"this":[73],"work,":[74],"we":[75],"present":[76],"a":[77,89],"MDCK":[78],"line":[80],"adapted":[81],"to":[82,136],"grow":[83],"single":[85],"with":[88],"doubling":[90],"time":[91],"less":[93],"than":[94],"20":[95],"h,":[96],"achieving":[97],"concentrations":[99,134],"over":[100],"1":[101],"\u00d7":[102,138],"107":[103,139],"cells/mL":[104],"batch":[106,114],"mode.":[107],"Influenza":[108],"A":[109],"titer":[111,143,150],"obtained":[112],"infections":[115],"were":[116,156],"3.6":[117],"log10(HAU/100":[118,146],"\u00b5L)":[119,147],"total-":[121],"109":[123],"virions/mL":[124,154],"infectious":[126,149],"particles":[128],"(TCID50),":[129],"respectively.":[130],"semi-perfusion":[132],"mode":[133],"up":[135],"6":[137],"cells/mL,":[140],"accumulated":[141],"4.5":[145],"almost":[152],"1010":[153],"(TCID50)":[155],"possible.":[157],"This":[158],"exceeds":[159],"results":[160],"reported":[161],"previously":[162],"culture-based":[165],"propagation":[168],"by":[169],"far":[170],"suggests":[172],"cultures":[174],"preferred":[177],"method":[178],"manufacturing.":[182]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":9},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":10},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2019-05-09T00:00:00"}
