{"id":"https://openalex.org/W2936720448","doi":null,"title":"Developing Gene Therapy Methods to Inhibit a BAG Family Co-chaperone Protein in Rat Substantia Nigra","display_name":"Developing Gene Therapy Methods to Inhibit a BAG Family Co-chaperone Protein in Rat Substantia Nigra","publication_year":2016,"publication_date":"2016-11-01","ids":{"openalex":"https://openalex.org/W2936720448","mag":"2936720448"},"language":"en","primary_location":{"id":"pmh:oai:utoronto.scholaris.ca:1807/93021","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1807/93021","pdf_url":"https://utoronto.scholaris.ca/bitstreams/479e46d9-f41b-4139-b873-6b4ec142d24d/download","source":{"id":"https://openalex.org/S4306400261","display_name":"TSpace (University of Toronto)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I185261750","host_organization_name":"University of Toronto","host_organization_lineage":["https://openalex.org/I185261750"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://utoronto.scholaris.ca/bitstreams/479e46d9-f41b-4139-b873-6b4ec142d24d/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5089729880","display_name":"Stanley Li","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Stanley Li","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5089729880"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.6679999828338623,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.6679999828338623,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10085","display_name":"Parkinson's Disease Mechanisms and Treatments","score":0.26510000228881836,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.02539999969303608,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/parkinson-s-disease","display_name":"Parkinson's disease","score":0.9010000228881836},{"id":"https://openalex.org/keywords/bag5","display_name":"BAG5","score":0.9929999709129333},{"id":"https://openalex.org/keywords/gene-therapy","display_name":"gene therapy","score":0.6669999957084656},{"id":"https://openalex.org/keywords/substantia-nigra","display_name":"substantia nigra","score":0.8920000195503235},{"id":"https://openalex.org/keywords/dopaminergic-neurons","display_name":"dopaminergic neurons","score":0.796999990940094},{"id":"https://openalex.org/keywords/shrna-knockdown","display_name":"shRNA knockdown","score":0.7799999713897705},{"id":"https://openalex.org/keywords/lentivirus","display_name":"lentivirus","score":0.6320000290870667},{"id":"https://openalex.org/keywords/adeno-associated-virus","display_name":"adeno-associated virus","score":0.75},{"id":"https://openalex.org/keywords/hsp70","display_name":"HSP70","score":0.8009999990463257},{"id":"https://openalex.org/keywords/parkin","display_name":"Parkin","score":0.9869999885559082},{"id":"https://openalex.org/keywords/neuroprotection","display_name":"neuroprotection","score":0.7379999756813049},{"id":"https://openalex.org/keywords/rat-model","display_name":"rat model","score":0.4830000102519989}],"concepts":[{"id":"https://openalex.org/C2780938664","wikidata":"https://www.wikidata.org/wiki/Q753278","display_name":"Substantia nigra","level":4,"score":0.9433658123016357},{"id":"https://openalex.org/C2775962898","wikidata":"https://www.wikidata.org/wiki/Q5073152","display_name":"Chaperone (clinical)","level":2,"score":0.48840588331222534},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.37269383668899536},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3545965552330017},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3538467288017273},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.3496435582637787},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.3330569267272949},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32944226264953613},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.32316499948501587},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.1719147264957428},{"id":"https://openalex.org/C513476851","wikidata":"https://www.wikidata.org/wiki/Q170304","display_name":"Dopamine","level":2,"score":0.09537702798843384},{"id":"https://openalex.org/C137183658","wikidata":"https://www.wikidata.org/wiki/Q461076","display_name":"Dopaminergic","level":3,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:utoronto.scholaris.ca:1807/93021","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1807/93021","pdf_url":"https://utoronto.scholaris.ca/bitstreams/479e46d9-f41b-4139-b873-6b4ec142d24d/download","source":{"id":"https://openalex.org/S4306400261","display_name":"TSpace (University of Toronto)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I185261750","host_organization_name":"University of Toronto","host_organization_lineage":["https://openalex.org/I185261750"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:2936720448","doi":null,"is_oa":false,"landing_page_url":"https://tspace.library.utoronto.ca/handle/1807/93021","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:utoronto.scholaris.ca:1807/93021","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1807/93021","pdf_url":"https://utoronto.scholaris.ca/bitstreams/479e46d9-f41b-4139-b873-6b4ec142d24d/download","source":{"id":"https://openalex.org/S4306400261","display_name":"TSpace (University of Toronto)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I185261750","host_organization_name":"University of Toronto","host_organization_lineage":["https://openalex.org/I185261750"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9763000011444092,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2936720448.pdf","grobid_xml":"https://content.openalex.org/works/W2936720448.grobid-xml"},"referenced_works_count":123,"referenced_works":["https://openalex.org/W70698837","https://openalex.org/W91327126","https://openalex.org/W593814261","https://openalex.org/W609346837","https://openalex.org/W655763762","https://openalex.org/W876571388","https://openalex.org/W906047497","https://openalex.org/W1486227004","https://openalex.org/W1494864007","https://openalex.org/W1586539673","https://openalex.org/W1606037334","https://openalex.org/W1626583747","https://openalex.org/W1655307540","https://openalex.org/W1767392319","https://openalex.org/W1918258073","https://openalex.org/W1964221220","https://openalex.org/W1967204443","https://openalex.org/W1967562856","https://openalex.org/W1974175558","https://openalex.org/W1976587845","https://openalex.org/W1976740826","https://openalex.org/W1977723295","https://openalex.org/W1982378942","https://openalex.org/W1982737877","https://openalex.org/W1987114379","https://openalex.org/W1987897718","https://openalex.org/W1991231925","https://openalex.org/W1993988048","https://openalex.org/W1998191613","https://openalex.org/W1999563094","https://openalex.org/W2006957728","https://openalex.org/W2007452808","https://openalex.org/W2012551702","https://openalex.org/W2014850190","https://openalex.org/W2019511637","https://openalex.org/W2020469175","https://openalex.org/W2022223015","https://openalex.org/W2024341039","https://openalex.org/W2024820040","https://openalex.org/W2025625712","https://openalex.org/W2027954222","https://openalex.org/W2029206837","https://openalex.org/W2030472096","https://openalex.org/W2030810287","https://openalex.org/W2032817175","https://openalex.org/W2034395013","https://openalex.org/W2037156284","https://openalex.org/W2042018401","https://openalex.org/W2042208155","https://openalex.org/W2042983080","https://openalex.org/W2044866051","https://openalex.org/W2045132404","https://openalex.org/W2045435533","https://openalex.org/W2047680643","https://openalex.org/W2048702149","https://openalex.org/W2049628420","https://openalex.org/W2051342143","https://openalex.org/W2052039425","https://openalex.org/W2052054514","https://openalex.org/W2052885679","https://openalex.org/W2058146246","https://openalex.org/W2058931009","https://openalex.org/W2059033731","https://openalex.org/W2061846521","https://openalex.org/W2065422132","https://openalex.org/W2066796446","https://openalex.org/W2071360006","https://openalex.org/W2075194152","https://openalex.org/W2077594155","https://openalex.org/W2081710098","https://openalex.org/W2084481001","https://openalex.org/W2085185328","https://openalex.org/W2086117912","https://openalex.org/W2087864583","https://openalex.org/W2089700848","https://openalex.org/W2091264505","https://openalex.org/W2091586455","https://openalex.org/W2092857499","https://openalex.org/W2093248461","https://openalex.org/W2093599867","https://openalex.org/W2093714761","https://openalex.org/W2097033526","https://openalex.org/W2099540110","https://openalex.org/W2105323510","https://openalex.org/W2106350754","https://openalex.org/W2106396251","https://openalex.org/W2113709638","https://openalex.org/W2122179784","https://openalex.org/W2127930801","https://openalex.org/W2128471322","https://openalex.org/W2128551987","https://openalex.org/W2130886644","https://openalex.org/W2135332827","https://openalex.org/W2135907949","https://openalex.org/W2140558141","https://openalex.org/W2142616950","https://openalex.org/W2142776810","https://openalex.org/W2143604504","https://openalex.org/W2154979583","https://openalex.org/W2156319424","https://openalex.org/W2157155969","https://openalex.org/W2166264384","https://openalex.org/W2166802172","https://openalex.org/W2168420463","https://openalex.org/W2168862665","https://openalex.org/W2195975231","https://openalex.org/W2200499641","https://openalex.org/W2210820223","https://openalex.org/W2227964848","https://openalex.org/W2257456801","https://openalex.org/W2263590114","https://openalex.org/W2281531600","https://openalex.org/W2299625896","https://openalex.org/W2318353205","https://openalex.org/W2340783927","https://openalex.org/W2345399459","https://openalex.org/W2402577214","https://openalex.org/W2404360023","https://openalex.org/W2414165069","https://openalex.org/W2414643134","https://openalex.org/W2416742466","https://openalex.org/W4211123396","https://openalex.org/W4241557582"],"related_works":["https://openalex.org/W2060458790","https://openalex.org/W2297170115","https://openalex.org/W1991231925","https://openalex.org/W2475432729","https://openalex.org/W2044866051","https://openalex.org/W2093949061","https://openalex.org/W2122688529","https://openalex.org/W7203603070","https://openalex.org/W2909713798","https://openalex.org/W4226251907"],"abstract_inverted_index":{"Parkinson\u00e2":[0],"s":[1],"disease":[2],"(PD)":[3],"is":[4,21],"a":[5,22,64,76],"progressive":[6],"neurodegenerative":[7],"disorder":[8],"affecting":[9],"dopaminergic":[10,26,35],"neurons":[11,131],"in":[12,29,46,58,96,116,146],"the":[13,73,138],"substantia":[14],"nigra":[15],"(SN).":[16],"Bcl2-associated":[17],"athanogene":[18],"5":[19],"(BAG5)":[20],"co-chaperone":[23],"that":[24,79,91],"promotes":[25],"cell":[27,98],"death":[28],"animal":[30,47],"models":[31,48,148],"of":[32,49,75,143,149],"PD":[33,59],"and":[34,41,51,100,106,129],"degeneration.":[36],"BAG5":[37,62,84,95,127,144],"also":[38],"inhibits":[39],"Hsp70":[40],"parkin,":[42],"which":[43],"are":[44],"protective":[45],"PD,":[50],"interacts":[52],"with":[53],"several":[54],"other":[55],"proteins":[56],"implicated":[57],"pathogenesis.":[60],"Thus,":[61],"represents":[63],"promising":[65],"therapeutic":[66],"target":[67],"for":[68,140],"PD.":[69,150],"This":[70,135],"thesis":[71],"describes":[72],"development":[74],"viral":[77],"vector":[78],"can":[80],"deliver":[81],"shRNA":[82],"against":[83],"to":[85,119],"rat":[86,122,147],"SN.":[87],"We":[88],"identified":[89],"shRNAs":[90],"efficiently":[92],"knocked":[93],"down":[94],"two":[97],"lines":[99],"packaged":[101],"them":[102],"into":[103],"lentivirus":[104],"(LV)":[105],"adeno-associated":[107],"virus":[108],"(AAV)":[109],"1/2":[110],"vectors.":[111],"AAV-shBAG5":[112,124],"demonstrated":[113],"superior":[114],"expression":[115],"SN":[117],"compared":[118],"LV.":[120],"In":[121],"SN,":[123],"produced":[125],"significant":[126],"knockdown":[128,145],"protected":[130],"from":[132],"axotomy":[133],"injury.":[134],"work":[136],"provides":[137],"foundation":[139],"future":[141],"studies":[142]},"counts_by_year":[],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2019-04-25T00:00:00"}