{"id":"https://openalex.org/W2933845386","doi":null,"title":"O-GlcNAc Regulates Erythroid Gene Transcription","display_name":"O-GlcNAc Regulates Erythroid Gene Transcription","publication_year":2017,"publication_date":"2017-08-31","ids":{"openalex":"https://openalex.org/W2933845386","mag":"2933845386"},"language":"en","primary_location":{"id":"pmh:oai:kuscholarworks.ku.edu:1808/25873","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/1808/25873","pdf_url":"http://hdl.handle.net/1808/25873","source":{"id":"https://openalex.org/S4377196285","display_name":"KU ScholarWorks (University of Kansas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I146416000","host_organization_name":"University of Kansas","host_organization_lineage":["https://openalex.org/I146416000"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/1808/25873","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100733120","display_name":"Zhen Zhang","orcid":"https://orcid.org/0000-0002-4864-6180","observed_orcids":["https://orcid.org/0000-0002-4864-6180"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Zhen Zhang","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-4864-6180","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5100733120"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11884","display_name":"Redox biology and oxidative stress","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/o-glcnac","display_name":"O-GlcNAc","score":0.9089999794960022},{"id":"https://openalex.org/keywords/o-glcnac-transferase","display_name":"O-GlcNAc transferase","score":0.8709999918937683},{"id":"https://openalex.org/keywords/o-glcnacase","display_name":"O-GlcNAcase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/thiamet-g","display_name":"Thiamet G","score":0.8119999766349792},{"id":"https://openalex.org/keywords/gata1","display_name":"GATA-1","score":0.9369999766349792},{"id":"https://openalex.org/keywords/fog-1","display_name":"FOG-1","score":0.8740000128746033},{"id":"https://openalex.org/keywords/erythropoiesis","display_name":"erythropoiesis","score":0.8659999966621399},{"id":"https://openalex.org/keywords/rna-polymerase-ii-occupancy","display_name":"RNA polymerase II occupancy","score":0.5189999938011169}],"concepts":[{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6349455118179321},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.5944845676422119},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4483085870742798},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.428437739610672},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.41890814900398254},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4186272919178009},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:kuscholarworks.ku.edu:1808/25873","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/1808/25873","pdf_url":"http://hdl.handle.net/1808/25873","source":{"id":"https://openalex.org/S4377196285","display_name":"KU ScholarWorks (University of Kansas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I146416000","host_organization_name":"University of Kansas","host_organization_lineage":["https://openalex.org/I146416000"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},{"id":"mag:2933845386","doi":null,"is_oa":false,"landing_page_url":"https://kuscholarworks.ku.edu/handle/1808/25873","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:kuscholarworks.ku.edu:1808/25873","doi":null,"is_oa":true,"landing_page_url":"https://hdl.handle.net/1808/25873","pdf_url":"http://hdl.handle.net/1808/25873","source":{"id":"https://openalex.org/S4377196285","display_name":"KU ScholarWorks (University of Kansas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I146416000","host_organization_name":"University of Kansas","host_organization_lineage":["https://openalex.org/I146416000"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.46810001134872437}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3592153889","display_name":"Nuclear Receptors in Liver Health and Disease","funder_award_id":"P20GM103549","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G4874150548","display_name":"Mechanisms of Liver Injury and Diseases","funder_award_id":"P30GM118247","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G5589523837","display_name":"Regulation of Globin Gene Switching by O-GlcNAc Post-Translational Modification","funder_award_id":"R01DK100595","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320310145","display_name":"Johns Hopkins University","ror":"https://ror.org/00za53h95"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2933845386.pdf","grobid_xml":"https://content.openalex.org/works/W2933845386.grobid-xml"},"referenced_works_count":114,"referenced_works":["https://openalex.org/W629466480","https://openalex.org/W1178570896","https://openalex.org/W1488598317","https://openalex.org/W1491060698","https://openalex.org/W1510239612","https://openalex.org/W1540964771","https://openalex.org/W1568866400","https://openalex.org/W1570460795","https://openalex.org/W1652316604","https://openalex.org/W1724131118","https://openalex.org/W1965962153","https://openalex.org/W1966629811","https://openalex.org/W1967556066","https://openalex.org/W1970056549","https://openalex.org/W1972576965","https://openalex.org/W1974515407","https://openalex.org/W1975518025","https://openalex.org/W1976523749","https://openalex.org/W1981460612","https://openalex.org/W1985739269","https://openalex.org/W1989476631","https://openalex.org/W1989561383","https://openalex.org/W1993052072","https://openalex.org/W1994396628","https://openalex.org/W1996310767","https://openalex.org/W1997853510","https://openalex.org/W1997968649","https://openalex.org/W1998906741","https://openalex.org/W1999478182","https://openalex.org/W2002888609","https://openalex.org/W2004123988","https://openalex.org/W2004373930","https://openalex.org/W2008035065","https://openalex.org/W2010623458","https://openalex.org/W2013020574","https://openalex.org/W2015357656","https://openalex.org/W2016015885","https://openalex.org/W2017548268","https://openalex.org/W2018639404","https://openalex.org/W2020990699","https://openalex.org/W2021814667","https://openalex.org/W2025129963","https://openalex.org/W2025589527","https://openalex.org/W2028224445","https://openalex.org/W2033357297","https://openalex.org/W2035689855","https://openalex.org/W2041744455","https://openalex.org/W2042233932","https://openalex.org/W2042922571","https://openalex.org/W2046345187","https://openalex.org/W2048666997","https://openalex.org/W2050575492","https://openalex.org/W2051948190","https://openalex.org/W2053677349","https://openalex.org/W2055568103","https://openalex.org/W2056173710","https://openalex.org/W2060744329","https://openalex.org/W2060799932","https://openalex.org/W2061999440","https://openalex.org/W2063859846","https://openalex.org/W2064347847","https://openalex.org/W2065955997","https://openalex.org/W2068787836","https://openalex.org/W2068972095","https://openalex.org/W2070033262","https://openalex.org/W2071520055","https://openalex.org/W2072135796","https://openalex.org/W2072307284","https://openalex.org/W2073229191","https://openalex.org/W2079499097","https://openalex.org/W2082889432","https://openalex.org/W2083682077","https://openalex.org/W2088231318","https://openalex.org/W2089723647","https://openalex.org/W2096040928","https://openalex.org/W2097670917","https://openalex.org/W2098632082","https://openalex.org/W2099766227","https://openalex.org/W2105214165","https://openalex.org/W2107279676","https://openalex.org/W2111373501","https://openalex.org/W2112965309","https://openalex.org/W2115862579","https://openalex.org/W2119499340","https://openalex.org/W2119816935","https://openalex.org/W2122617298","https://openalex.org/W2123106337","https://openalex.org/W2123347941","https://openalex.org/W2125695375","https://openalex.org/W2130410032","https://openalex.org/W2134581728","https://openalex.org/W2138867046","https://openalex.org/W2141340108","https://openalex.org/W2141373343","https://openalex.org/W2144792857","https://openalex.org/W2144814555","https://openalex.org/W2146527846","https://openalex.org/W2151396228","https://openalex.org/W2151625863","https://openalex.org/W2160508755","https://openalex.org/W2161894347","https://openalex.org/W2162198627","https://openalex.org/W2166305185","https://openalex.org/W2170127671","https://openalex.org/W2247766769","https://openalex.org/W2294840695","https://openalex.org/W2338308550","https://openalex.org/W2507874699","https://openalex.org/W2546967042","https://openalex.org/W2569978533","https://openalex.org/W2613539552","https://openalex.org/W2768474937","https://openalex.org/W6981637969","https://openalex.org/W6990656925"],"related_works":["https://openalex.org/W2028224445","https://openalex.org/W1495880270","https://openalex.org/W3097441864","https://openalex.org/W4405040916","https://openalex.org/W2905094310","https://openalex.org/W2940371136","https://openalex.org/W2982713380","https://openalex.org/W3095871033","https://openalex.org/W2884172031","https://openalex.org/W3174816906"],"abstract_inverted_index":{"O-GlcNAc":[0,12,24,36,61,76,110,134,232,430,479,523,613],"is":[1,13,63,83,249,271,288,427,449,513],"a":[2,51,450,492,500,534,540,590,635,639,650],"post-translational":[3],"modification":[4],"on":[5],"serine":[6,18],"or":[7,168,266,286],"threonine":[8,20],"residues":[9,21],"by":[10,22,102,238,319,458,515,625],"\u03b2-N-acetylglucosamine.":[11],"added":[14],"and":[15,19,29,48,80,117,140,181,234,297,337,361,388,399,431,434,440,539,550,564,608,610,646],"removed":[16],"from":[17,255],"the":[23,45,66,73,94,98,113,172,200,210,250,272,278,311,324,329,341,345,353,383,391,405,460,463,468,473,516,604,611],"processing":[25],"enzymes,":[26],"O-GlcNAc-transferase":[27],"(OGT)":[28],"O-GlcNAcase":[30],"(OGA),":[31],"respectively.":[32],"The":[33],"levels":[34,135,524],"of":[35,68,93,100,115,174,252,352,385,453,462,496,518,558,592,606,641],"can":[37],"rapidly":[38],"change":[39],"in":[40,44,109,137,171,231,365,412,530,537,542,637],"response":[41],"to":[42,50,65,90,131,229,304,356,578],"fluctuations":[43],"extracellular":[46],"environment":[47],"return":[49],"baseline":[52],"level":[53,147,614],"quickly":[54],"after":[55,216,527],"stimulus":[56],"removal.":[57],"This":[58],"process":[59,251],"termed":[60],"homeostasis":[62,111,233],"critical":[64],"regulation":[67],"many":[69],"cellular":[70],"functions.":[71],"However,":[72,277],"relationship":[74],"between":[75],"homeostasis,":[77],"OGT,":[78,386],"OGA,":[79,387],"gene":[81,284,313,331,455,483],"transcription":[82,101,114,146,183,190,211,226,484],"unknown.":[84],"In":[85,396,487],"this":[86,260,306,488,551],"dissertation,":[87],"I":[88,105],"seek":[89],"address":[91,305],"some":[92],"fundamental":[95],"mechanisms":[96],"into":[97],"control":[99],"O-GlcNAcylation.":[103,599],"First,":[104],"explored":[106],"how":[107,243,281],"changes":[108,230],"affect":[112],"OGT":[116,139,145,167,180,336,360,398,433,543],"OGA.":[118],"We":[119,308,333],"treated":[120,187,376,561],"several":[121],"human":[122,416],"cell":[123,296,494],"lines":[124],"with":[125,154,159,188,300,340,377,429,437,533,548,562],"Thiamet-G":[126,381,565],"(TMG,":[127,566],"an":[128,378,567],"OGA":[129,142,151,169,182,201,225,301,338,362,379,435,538,568,622],"inhibitor)":[130,569],"increase":[132,536],"overall":[133,522,612],"resulting":[136],"decreased":[138,526,620],"increased":[141,153,403,553],"protein":[143,160,170,544],"expression.":[144],"slightly":[148],"declined,":[149],"but":[150],"significantly":[152,358],"TMG":[155,178,218,303,626],"treatment.":[156,219],"Pretreating":[157],"cells":[158,185,369,560],"translation":[161],"inhibitor":[162,191,302,380],"cycloheximide":[163],"did":[164,177],"not":[165,289],"stabilize":[166,179],"presence":[173],"TMG;":[175],"nor":[176],"when":[184,408,510,621],"were":[186],"RNA":[189,194,205],"actinomycin":[192],"D.":[193],"Pol":[195,206],"II":[196,207],"chromatin":[197],"immunoprecipitation":[198],"at":[199,209,315,323,328,344,390,404,467,615,655],"promoter":[202,343,363,393,407],"showed":[203,424],"that":[204,224,335,425,447,506,571,603,632],"occupancy":[208,384,605],"start":[212],"site":[213,327,355,619],"was":[214,227,235,394,402,623],"lower":[215],"prolonged":[217],"Together,":[220],"these":[221],"data":[222,445,588,630],"suggest":[223,589,647],"sensitive":[228],"potentially":[236],"regulated":[237,597],"O-GlcNAc.":[239],"Next,":[240,600],"we":[241,293,423,476,490,601],"investigated":[242],"O-GlcNAcylation":[244,448,633,648],"regulates":[245,480],"A\u03b3-globin":[246,312,330,342,392,406],"transcription.":[247],"Erythropoiesis":[248],"generating":[253],"erythrocytes":[254],"erythroid":[256,508,644],"progenitor":[257],"cells.":[258],"During":[259],"process,":[261],"numerous":[262],"genes":[263,573,595,645],"are":[264,375,596],"up-regulated":[265],"down-regulated.":[267],"Transcription":[268],"factor":[269],"GATA-1":[270,282,481,502,511,528,546,584,593,607,642,653,657],"master":[273],"regulator":[274],"for":[275],"erythropoiesis.":[276,486,555,628],"mechanism":[279,452,651],"underling":[280],"regulate":[283],"activation":[285,529],"repression":[287,314],"well":[290],"understood.":[291],"Here,":[292],"utilized":[294,491],"different":[295],"animal":[298],"models":[299],"question.":[307],"first":[309],"studied":[310],"adult":[316],"stage":[317],"mediated":[318,457],"GATA-1/FOG-1/Mi2\u03b2":[320,464],"repressor":[321,348,465],"complex":[322,466],"-566":[325,346,469],"GATA":[326,347,354,470,617],"promoter.":[332,474],"demonstrated":[334,602],"interact":[339],"site;":[349],"however,":[350],"mutation":[351],"GAGA":[357],"reduced":[359],"interactions":[364],"\u03b2-YAC":[366,373,417],"bone":[367],"marrow":[368],"(BMCs).":[370],"When":[371],"WT":[372],"BMCs":[374],"(TMG),":[382],"Mi2\u03b2":[389,400,426],"increased.":[395],"addition,":[397],"recruitment":[401],"\u03b3-globin":[409,454],"becomes":[410],"repressed":[411],"post-conception":[413],"day":[414],"E18":[415],"transgenic":[418],"mouse":[419],"fetal":[420],"liver.":[421],"Furthermore,":[422],"modified":[428],"both":[432],"interacts":[436,547],"Mi2\u03b2,":[438],"GATA-1,":[439],"FOG-1.":[441],"Taken":[442],"together,":[443],"our":[444],"suggested":[446],"novel":[451],"regulation,":[456],"modulating":[459],"assembly":[461],"motif":[471],"within":[472],"Then,":[475],"asked":[477],"if":[478],"target":[482,585,594],"during":[485,554,627],"study,":[489],"well-established":[493],"model":[495],"erythropoiesis,":[497],"G1E-ER4":[498,531,559],"cells,":[499,532],"murine":[501],"null":[503],"erythroblast":[504],"line":[505],"undergoes":[507],"differentiation":[509],"activity":[512],"restored":[514],"addition":[517],"\u03b2-estradiol":[519],"(E2).":[520],"Interestingly,":[521],"dramatically":[525],"slight":[535],"decrease":[541],"levels.":[545],"OGT/OGA,":[549,609],"interaction":[552],"Transcriptome":[556],"analysis":[557],"E2":[563,579],"revealed":[570],"1,173":[572],"changed":[574],"expression":[575],"patterns":[576],"compared":[577],"treatment":[580],"only,":[581],"including":[582],"433":[583],"genes.":[586,659],"These":[587],"subset":[591,640],"through":[598],"Laptm5":[616],"binding":[618],"inhibited":[624],"Our":[629],"suggests":[631],"plays":[634],"role":[636],"regulating":[638,652],"targeted":[643,658],"as":[649],"function":[654],"specific":[656]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2019-04-11T00:00:00"}
