{"id":"https://openalex.org/W29241963","doi":null,"title":"The role of the intermediate filament (nanofilament) protein nestin in neural progenitor cell and astrocyte differentiation","display_name":"The role of the intermediate filament (nanofilament) protein nestin in neural progenitor cell and astrocyte differentiation","publication_year":2014,"publication_date":"2014-05-23","ids":{"openalex":"https://openalex.org/W29241963","mag":"29241963"},"language":"en","primary_location":{"id":"pmh:oai:gupea.ub.gu.se:2077/35462","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2077/35462","pdf_url":"http://hdl.handle.net/2077/35462","source":{"id":"https://openalex.org/S4306401168","display_name":"Gothenburg University Publications Electronic Archive (Gothenburg University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I881427289","host_organization_name":"University of Gothenburg","host_organization_lineage":["https://openalex.org/I881427289"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Doctoral thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/2077/35462","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038124339","display_name":"Isabell Lebkuechner","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I881427289","display_name":"University of Gothenburg","ror":"https://ror.org/01tm6cn81","country_code":"SE","type":"education","lineage":["https://openalex.org/I881427289"]}],"countries":["SE"],"is_corresponding":true,"raw_author_name":"Isabell Lebkuechner","raw_affiliation_strings":["University of Gothenburg"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Gothenburg","institution_ids":["https://openalex.org/I881427289"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5038124339"],"corresponding_institution_ids":["https://openalex.org/I881427289"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11266","display_name":"Neuroinflammation and Neurodegeneration Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nestin","display_name":"nestin","score":1.0},{"id":"https://openalex.org/keywords/intermediate-filaments","display_name":"intermediate filaments","score":0.9340000152587891},{"id":"https://openalex.org/keywords/neural-stem-progenitor-cells","display_name":"neural stem/progenitor cells","score":0.9319999814033508},{"id":"https://openalex.org/keywords/astrocyte-differentiation","display_name":"astrocyte differentiation","score":0.7710000276565552},{"id":"https://openalex.org/keywords/reactive-astrocytes","display_name":"reactive astrocytes","score":0.8019999861717224},{"id":"https://openalex.org/keywords/astrocyte-heterogeneity","display_name":"astrocyte heterogeneity","score":0.8399999737739563},{"id":"https://openalex.org/keywords/notch-signaling","display_name":"Notch signaling","score":0.9779999852180481},{"id":"https://openalex.org/keywords/jagged1","display_name":"Jagged1","score":0.953000009059906},{"id":"https://openalex.org/keywords/hb-egf","display_name":"HB-EGF","score":0.8740000128746033},{"id":"https://openalex.org/keywords/glial-fibrillary-acidic-protein","display_name":"glial fibrillary acidic protein","score":0.7379999756813049},{"id":"https://openalex.org/keywords/vimentin","display_name":"vimentin","score":1.0},{"id":"https://openalex.org/keywords/hippocampal-neurogenesis","display_name":"hippocampal neurogenesis","score":0.8370000123977661},{"id":"https://openalex.org/keywords/astrocyte-subpopulations","display_name":"astrocyte subpopulations","score":0.8119999766349792}],"concepts":[{"id":"https://openalex.org/C2777738006","wikidata":"https://www.wikidata.org/wiki/Q14906812","display_name":"Nestin","level":4,"score":0.9153424501419067},{"id":"https://openalex.org/C4746552","wikidata":"https://www.wikidata.org/wiki/Q1456827","display_name":"Neurogenesis","level":2,"score":0.7855225205421448},{"id":"https://openalex.org/C2777542381","wikidata":"https://www.wikidata.org/wiki/Q502961","display_name":"Astrocyte","level":3,"score":0.6906075477600098},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6274707317352295},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6181399822235107},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.6126435399055481},{"id":"https://openalex.org/C78383274","wikidata":"https://www.wikidata.org/wiki/Q421987","display_name":"Intermediate filament","level":4,"score":0.6014886498451233},{"id":"https://openalex.org/C161879069","wikidata":"https://www.wikidata.org/wiki/Q904082","display_name":"Notch signaling pathway","level":3,"score":0.6012549996376038},{"id":"https://openalex.org/C136834591","wikidata":"https://www.wikidata.org/wiki/Q2944097","display_name":"Neural stem cell","level":3,"score":0.5793120861053467},{"id":"https://openalex.org/C2780495277","wikidata":"https://www.wikidata.org/wiki/Q905442","display_name":"Glial fibrillary acidic protein","level":3,"score":0.5757930278778076},{"id":"https://openalex.org/C48261664","wikidata":"https://www.wikidata.org/wiki/Q14908218","display_name":"Gliogenesis","level":4,"score":0.5096244812011719},{"id":"https://openalex.org/C147447768","wikidata":"https://www.wikidata.org/wiki/Q411624","display_name":"Vimentin","level":3,"score":0.45858997106552124},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.34708982706069946},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.27931949496269226},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.22498497366905212},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1951422393321991},{"id":"https://openalex.org/C529278444","wikidata":"https://www.wikidata.org/wiki/Q47273","display_name":"Central nervous system","level":2,"score":0.14978867769241333},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.14916777610778809},{"id":"https://openalex.org/C142669718","wikidata":"https://www.wikidata.org/wiki/Q154626","display_name":"Cytoskeleton","level":3,"score":0.09471362829208374},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.0829923152923584},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.06016811728477478}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:gupea.ub.gu.se:2077/35462","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2077/35462","pdf_url":"http://hdl.handle.net/2077/35462","source":{"id":"https://openalex.org/S4306401168","display_name":"Gothenburg University Publications Electronic Archive (Gothenburg University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I881427289","host_organization_name":"University of Gothenburg","host_organization_lineage":["https://openalex.org/I881427289"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Doctoral thesis"},{"id":"mag:29241963","doi":null,"is_oa":false,"landing_page_url":"https://gupea.ub.gu.se/bitstream/2077/35462/2/gupea_2077_35462_2.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:gupea.ub.gu.se:2077/35462","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2077/35462","pdf_url":"http://hdl.handle.net/2077/35462","source":{"id":"https://openalex.org/S4306401168","display_name":"Gothenburg University Publications Electronic Archive (Gothenburg University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I881427289","host_organization_name":"University of Gothenburg","host_organization_lineage":["https://openalex.org/I881427289"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Doctoral thesis"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8371999859809875,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322168","display_name":"Hj\u00e4rnfonden","ror":"https://ror.org/014btj409"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W29241963.pdf","grobid_xml":"https://content.openalex.org/works/W29241963.grobid-xml"},"referenced_works_count":208,"referenced_works":["https://openalex.org/W56999605","https://openalex.org/W109891194","https://openalex.org/W141378801","https://openalex.org/W1484717592","https://openalex.org/W1487222554","https://openalex.org/W1504164986","https://openalex.org/W1513166852","https://openalex.org/W1540655326","https://openalex.org/W1548862642","https://openalex.org/W1549972124","https://openalex.org/W1551925364","https://openalex.org/W1561692645","https://openalex.org/W1581458838","https://openalex.org/W1768187423","https://openalex.org/W1840213669","https://openalex.org/W1859730138","https://openalex.org/W1942886943","https://openalex.org/W1963679906","https://openalex.org/W1964017356","https://openalex.org/W1964493811","https://openalex.org/W1965676841","https://openalex.org/W1965976131","https://openalex.org/W1966345556","https://openalex.org/W1967238041","https://openalex.org/W1967762149","https://openalex.org/W1967896094","https://openalex.org/W1968235367","https://openalex.org/W1969305530","https://openalex.org/W1969854375","https://openalex.org/W1971571526","https://openalex.org/W1972167202","https://openalex.org/W1974372416","https://openalex.org/W1974379562","https://openalex.org/W1974689368","https://openalex.org/W1974932537","https://openalex.org/W1977619993","https://openalex.org/W1978813800","https://openalex.org/W1980499453","https://openalex.org/W1983197885","https://openalex.org/W1987145582","https://openalex.org/W1988044590","https://openalex.org/W1988866780","https://openalex.org/W1988976106","https://openalex.org/W1989732890","https://openalex.org/W1990161015","https://openalex.org/W1991701465","https://openalex.org/W1992521533","https://openalex.org/W1992715617","https://openalex.org/W1993408609","https://openalex.org/W1994622161","https://openalex.org/W1995242599","https://openalex.org/W1996241541","https://openalex.org/W1996340200","https://openalex.org/W1997223338","https://openalex.org/W2000488860","https://openalex.org/W2000770398","https://openalex.org/W2001306254","https://openalex.org/W2003410399","https://openalex.org/W2003798909","https://openalex.org/W2004251958","https://openalex.org/W2005048186","https://openalex.org/W2006289803","https://openalex.org/W2007114154","https://openalex.org/W2008183439","https://openalex.org/W2011299866","https://openalex.org/W2011331227","https://openalex.org/W2013485257","https://openalex.org/W2014490965","https://openalex.org/W2015445474","https://openalex.org/W2015541518","https://openalex.org/W2017200573","https://openalex.org/W2017946350","https://openalex.org/W2019610319","https://openalex.org/W2020138925","https://openalex.org/W2020521634","https://openalex.org/W2020885687","https://openalex.org/W2021551531","https://openalex.org/W2022345246","https://openalex.org/W2022487066","https://openalex.org/W2022600603","https://openalex.org/W2026147918","https://openalex.org/W2028948697","https://openalex.org/W2030508161","https://openalex.org/W2030591425","https://openalex.org/W2031611126","https://openalex.org/W2033794046","https://openalex.org/W2039326270","https://openalex.org/W2041033879","https://openalex.org/W2041104744","https://openalex.org/W2043828229","https://openalex.org/W2044110964","https://openalex.org/W2046006021","https://openalex.org/W2046756882","https://openalex.org/W2047585900","https://openalex.org/W2047755130","https://openalex.org/W2048064609","https://openalex.org/W2048734728","https://openalex.org/W2049789112","https://openalex.org/W2050382124","https://openalex.org/W2051021067","https://openalex.org/W2052087486","https://openalex.org/W2054467590","https://openalex.org/W2055129010","https://openalex.org/W2055169742","https://openalex.org/W2055867169","https://openalex.org/W2055891615","https://openalex.org/W2057269156","https://openalex.org/W2057608998","https://openalex.org/W2058693333","https://openalex.org/W2059143929","https://openalex.org/W2059201000","https://openalex.org/W2063498603","https://openalex.org/W2063580615","https://openalex.org/W2063692131","https://openalex.org/W2065569210","https://openalex.org/W2065776374","https://openalex.org/W2066104198","https://openalex.org/W2068400560","https://openalex.org/W2071696504","https://openalex.org/W2071802665","https://openalex.org/W2072320390","https://openalex.org/W2073345047","https://openalex.org/W2073390876","https://openalex.org/W2074467596","https://openalex.org/W2074661552","https://openalex.org/W2074717269","https://openalex.org/W2075119539","https://openalex.org/W2076432328","https://openalex.org/W2077070290","https://openalex.org/W2079156156","https://openalex.org/W2079356804","https://openalex.org/W2080361651","https://openalex.org/W2081399863","https://openalex.org/W2081895884","https://openalex.org/W2082329019","https://openalex.org/W2083663126","https://openalex.org/W2083904945","https://openalex.org/W2084566588","https://openalex.org/W2084629412","https://openalex.org/W2084652820","https://openalex.org/W2085424855","https://openalex.org/W2087700804","https://openalex.org/W2088906043","https://openalex.org/W2089216837","https://openalex.org/W2092967807","https://openalex.org/W2095812114","https://openalex.org/W2096327192","https://openalex.org/W2097712502","https://openalex.org/W2098622411","https://openalex.org/W2100448566","https://openalex.org/W2101233757","https://openalex.org/W2102035192","https://openalex.org/W2102089332","https://openalex.org/W2104230351","https://openalex.org/W2105733113","https://openalex.org/W2107561896","https://openalex.org/W2108244474","https://openalex.org/W2112982595","https://openalex.org/W2116447221","https://openalex.org/W2117256328","https://openalex.org/W2118661818","https://openalex.org/W2119321274","https://openalex.org/W2120767341","https://openalex.org/W2121076016","https://openalex.org/W2122223946","https://openalex.org/W2122412289","https://openalex.org/W2123048941","https://openalex.org/W2123426485","https://openalex.org/W2123609069","https://openalex.org/W2124021558","https://openalex.org/W2124658196","https://openalex.org/W2124940469","https://openalex.org/W2125236750","https://openalex.org/W2127338217","https://openalex.org/W2128229230","https://openalex.org/W2129567809","https://openalex.org/W2132023472","https://openalex.org/W2132504992","https://openalex.org/W2133943800","https://openalex.org/W2135046848","https://openalex.org/W2139481908","https://openalex.org/W2139576028","https://openalex.org/W2139646621","https://openalex.org/W2140732424","https://openalex.org/W2140998457","https://openalex.org/W2141838148","https://openalex.org/W2142401354","https://openalex.org/W2143000970","https://openalex.org/W2145791605","https://openalex.org/W2146856737","https://openalex.org/W2147241696","https://openalex.org/W2147270524","https://openalex.org/W2150861078","https://openalex.org/W2151643037","https://openalex.org/W2152460583","https://openalex.org/W2153591334","https://openalex.org/W2154072118","https://openalex.org/W2155661160","https://openalex.org/W2160779535","https://openalex.org/W2161289376","https://openalex.org/W2163556487","https://openalex.org/W2164142213","https://openalex.org/W2165224884","https://openalex.org/W2166851454","https://openalex.org/W2169357003","https://openalex.org/W2171339171","https://openalex.org/W2172266256","https://openalex.org/W2417121085"],"related_works":["https://openalex.org/W1481374374","https://openalex.org/W2907398689","https://openalex.org/W2979988787","https://openalex.org/W2132504992","https://openalex.org/W2063208633","https://openalex.org/W2153591334","https://openalex.org/W2060025069","https://openalex.org/W1987145582","https://openalex.org/W2066262464","https://openalex.org/W2119624278"],"abstract_inverted_index":{"Nestin,":[0],"a":[1,12,50,108,115,129,175,261],"class":[2],"VI":[3],"intermediate":[4,38],"filament":[5,39],"(nanofilament)":[6],"protein,":[7],"is":[8,27,49,178],"commonly":[9],"used":[10,182,302],"as":[11,136,138,236],"marker":[13],"for":[14,72],"neural":[15],"stem/progenitor":[16],"cells":[17,126],"(NSPCs),":[18],"but":[19,209],"its":[20],"role":[21],"in":[22,30,59,86,102,114,118,134,241,288,293],"neurogenesis":[23,152],"remains":[24],"elusive.":[25],"Nestin":[26],"also":[28,99],"expressed":[29],"immature":[31],"and":[32,47,127,150,220,227,247,270,278,298],"reactive":[33,54],"astrocytes.":[34],"The":[35],"up-regulation":[36],"of":[37,53,62,77,94,121,131,148,157,167,171,192,215,238,272,284,296],"proteins":[40],"glial":[41],"fibrillary":[42],"acidic":[43],"protein":[44],"(GFAP),":[45],"vimentin":[46],"nestin":[48,73,104,226,285],"characteristic":[51,147],"feature":[52],"astrocytes,":[55],"accompanied":[56],"by":[57,153],"alterations":[58],"the":[60,164,190,205,281],"expression":[61,271],"many":[63],"other":[64],"genes.":[65],"We":[66,98,181],"found":[67],"that":[68,101,112,204,225,255],"transgenic":[69],"mice":[70],"deficient":[71,105],"have":[74],"increased":[75,90],"number":[76],"newly":[78,95,244],"born":[79,96],"hippocampal":[80],"neurons":[81],"6":[82],"weeks":[83],"after":[84],"BrdU":[85],"vivo":[87],"labeling,":[88],"suggesting":[89],"generation":[91],"and/or":[92],"survival":[93],"neurons.":[97],"showed":[100,224,254],"vitro":[103],"astrocytes":[106,143,193,216,297],"provide":[107],"more":[109,262],"pro-neurogenic":[110],"environment":[111],"results":[113,202,292],"2-fold":[116],"increase":[117],"neuronal":[119,155],"differentiation":[120,156,269],"NSPCs.\\nAstrocytes":[122],"are":[123],"highly":[124,179],"heterogeneous":[125],"fulfill":[128],"variety":[130],"important":[132],"functions":[133],"healthy":[135],"well":[137],"diseased":[139],"brain.":[140],"In":[141],"addition,":[142],"can":[144],"exhibit":[145],"features":[146],"NSPCs":[149,158],"modulate":[151],"inhibiting":[154],"through":[159],"Jagged1-mediated":[160],"Notch":[161,198,206,211,273],"signaling.":[162],"Given":[163],"vast":[165],"array":[166],"astrocyte":[168,172,239,258],"functions,":[169],"classification":[170],"subpopulations":[173,240],"on":[174,218],"molecular":[176],"level":[177],"desirable.":[180],"single":[183],"cell":[184,250,289],"quantitative":[185],"real-time":[186],"PCR":[187],"to":[188,196,280],"investigate":[189],"heterogeneity":[191],"with":[194,303],"respect":[195],"their":[197],"signaling":[199],"competence.":[200],"Our":[201],"show":[203],"signal":[207,212,274],"sending":[208],"not":[210],"receiving":[213],"competence":[214],"depends":[217],"GFAP":[219],"vimentin.":[221],"Further,":[222],"we":[223,253],"heparin":[228],"binding":[229],"EGF-like":[230],"growth":[231],"factor":[232],"(HB-EGF)":[233],"may":[234],"serve":[235],"classifiers":[237],"vitro.\\nUtilizing":[242],"our":[243],"developed":[245],"Bioactive3D":[246],"standard":[248],"2D":[249],"culture":[251,290],"systems,":[252],"HB-EGF":[256,266,287],"alters":[257],"morphology":[259],"towards":[260],"radial":[263],"glia-like":[264],"phenotype.":[265],"affects":[267],"proliferation,":[268],"pathway":[275],"related":[276],"genes":[277],"leads":[279],"up":[282],"regulation":[283],"expression.":[286],"media":[291],"partial":[294],"de-differentiation":[295],"therefore":[299],"should":[300],"be":[301],"caution.":[304]},"counts_by_year":[],"updated_date":"2026-09-26T08:19:03.415552","created_date":"2016-06-24T00:00:00"}