{"id":"https://openalex.org/W2920388272","doi":"https://doi.org/10.1101/pdb.prot100933","title":"Measurement of Radioactivity in Nucleic Acids","display_name":"Measurement of Radioactivity in Nucleic Acids","publication_year":2019,"publication_date":"2019-03-01","ids":{"openalex":"https://openalex.org/W2920388272","doi":"https://doi.org/10.1101/pdb.prot100933","mag":"2920388272","pmid":"https://pubmed.ncbi.nlm.nih.gov/30824626"},"language":"en","primary_location":{"id":"doi:10.1101/pdb.prot100933","doi":"https://doi.org/10.1101/pdb.prot100933","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot100933","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060605798","display_name":"Michael R. Green","orcid":"https://orcid.org/0000-0003-3017-3298","observed_orcids":["https://orcid.org/0000-0003-3017-3298","https://orcid.org/0000-0001-6309-9472"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Michael R. Green","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5112152140","display_name":"Joseph Sambrook","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Joseph Sambrook","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"2019","issue":"3","first_page":"pdb.prot100933","last_page":"pdb.prot100933"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.6021000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.6021000146865845,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13414","display_name":"Radioactive Decay and Measurement Techniques","score":0.1906999945640564,"subfield":{"id":"https://openalex.org/subfields/3108","display_name":"Radiation"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.1597999930381775,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/radioactivity-measurement","display_name":"radioactivity measurement","score":0.8980000019073486},{"id":"https://openalex.org/keywords/nucleic-acids","display_name":"nucleic acids","score":0.8460000157356262},{"id":"https://openalex.org/keywords/radioactive-tracers","display_name":"radioactive tracers","score":0.6949999928474426},{"id":"https://openalex.org/keywords/dna-synthesis","display_name":"DNA synthesis","score":0.8270000219345093},{"id":"https://openalex.org/keywords/rna-synthesis","display_name":"RNA synthesis","score":1.0},{"id":"https://openalex.org/keywords/trichloroacetic-acid-precipitation","display_name":"trichloroacetic acid precipitation","score":0.890999972820282},{"id":"https://openalex.org/keywords/reaction-efficiency","display_name":"reaction efficiency","score":0.5580000281333923}],"concepts":[{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.8775012493133545},{"id":"https://openalex.org/C2777332483","wikidata":"https://www.wikidata.org/wiki/Q410116","display_name":"Trichloroacetic acid","level":2,"score":0.8061550855636597},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5735934972763062},{"id":"https://openalex.org/C177322064","wikidata":"https://www.wikidata.org/wiki/Q750955","display_name":"Radiochemistry","level":1,"score":0.538276731967926},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5213543176651001},{"id":"https://openalex.org/C107054158","wikidata":"https://www.wikidata.org/wiki/Q25257","display_name":"Precipitation","level":2,"score":0.5023257732391357},{"id":"https://openalex.org/C164304813","wikidata":"https://www.wikidata.org/wiki/Q25276","display_name":"Isotope","level":2,"score":0.49124354124069214},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.44122999906539917},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.28253844380378723},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.09349003434181213},{"id":"https://openalex.org/C185544564","wikidata":"https://www.wikidata.org/wiki/Q81197","display_name":"Nuclear physics","level":1,"score":0.07751163840293884},{"id":"https://openalex.org/C153294291","wikidata":"https://www.wikidata.org/wiki/Q25261","display_name":"Meteorology","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011232","descriptor_name":"Chemical Precipitation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011868","descriptor_name":"Radioisotopes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D014238","descriptor_name":"Trichloroacetic Acid","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1101/pdb.prot100933","doi":"https://doi.org/10.1101/pdb.prot100933","is_oa":false,"landing_page_url":"https://doi.org/10.1101/pdb.prot100933","pdf_url":null,"source":{"id":"https://openalex.org/S119953358","display_name":"Cold Spring Harbor Protocols","issn_l":"1559-6095","issn":["1559-6095","1940-3402"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315909","host_organization_name":"Cold Spring Harbor Laboratory Press","host_organization_lineage":["https://openalex.org/P4310315909"],"host_organization_lineage_names":["Cold Spring Harbor Laboratory Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor Protocols","raw_type":"journal-article"},{"id":"pmid:30824626","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30824626","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cold Spring Harbor protocols","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2412080297","https://openalex.org/W2012797345","https://openalex.org/W2085711230","https://openalex.org/W2474379374","https://openalex.org/W2418815730","https://openalex.org/W2994308497","https://openalex.org/W2097743280","https://openalex.org/W2094286281","https://openalex.org/W2412218684","https://openalex.org/W2025742247"],"abstract_inverted_index":{"Radioactive":[0],"isotopes":[1],"are":[2],"used":[3,13],"as":[4],"tracers":[5],"to":[6,14,29,46],"monitor":[7],"the":[8,21,32,35,40,54],"progress":[9],"of":[10,23,34,53],"many":[11],"reactions":[12],"synthesize":[15],"DNA":[16],"and":[17],"RNA.":[18],"To":[19],"calculate":[20],"efficiency":[22],"such":[24],"reactions,":[25],"it":[26],"is":[27,50],"necessary":[28],"measure":[30],"accurately":[31],"proportion":[33],"radioactive":[36],"precursor":[37],"incorporated":[38],"into":[39],"desired":[41],"product.":[42],"An":[43],"effective":[44],"method":[45],"achieve":[47],"this":[48],"goal":[49],"differential":[51],"precipitation":[52],"nucleic":[55],"acid":[56,60],"products":[57],"with":[58],"trichloroacetic":[59],"(TCA).":[61]},"counts_by_year":[{"year":2019,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2019-03-11T00:00:00"}