{"id":"https://openalex.org/W2918704602","doi":"https://doi.org/10.1161/res.123.suppl_1.461","title":"Abstract 461: Prospective Isolation of Heart Field Specific Cardiomyocytes from Differentiating Human Embryonic Stem Cells","display_name":"Abstract 461: Prospective Isolation of Heart Field Specific Cardiomyocytes from Differentiating Human Embryonic Stem Cells","publication_year":2018,"publication_date":"2018-08-03","ids":{"openalex":"https://openalex.org/W2918704602","doi":"https://doi.org/10.1161/res.123.suppl_1.461","mag":"2918704602"},"language":"en","primary_location":{"id":"doi:10.1161/res.123.suppl_1.461","doi":"https://doi.org/10.1161/res.123.suppl_1.461","is_oa":false,"landing_page_url":"https://doi.org/10.1161/res.123.suppl_1.461","pdf_url":null,"source":{"id":"https://openalex.org/S10652568","display_name":"Circulation Research","issn_l":"0009-7330","issn":["0009-7330","1524-4571"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Circulation Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021519262","display_name":"James Douglas Engel","orcid":"https://orcid.org/0000-0002-7512-8127","observed_orcids":["https://orcid.org/0000-0002-7512-8127"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James L Engel","raw_affiliation_strings":["Univ of California, Los Angeles, Los Angeles, CA","James L Engel"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ of California, Los Angeles, Los Angeles, CA","institution_ids":["https://openalex.org/I161318765"]},{"raw_affiliation_string":"James L Engel","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077273906","display_name":"Arash Pezhouman","orcid":"https://orcid.org/0000-0001-9106-7136","observed_orcids":["https://orcid.org/0000-0001-9106-7136"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Arash Pezhouman","raw_affiliation_strings":["Univ of California, Los Angeles, Los Angeles, CA","Arash Pezhouman"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ of California, Los Angeles, Los Angeles, CA","institution_ids":["https://openalex.org/I161318765"]},{"raw_affiliation_string":"Arash Pezhouman","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103115061","display_name":"Ngoc B. Nguyen","orcid":"https://orcid.org/0000-0002-4602-6586","observed_orcids":["https://orcid.org/0000-0002-4602-6586"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ngoc B Nguyen","raw_affiliation_strings":["Univ of California, Los Angeles, Los Angeles, CA","Ngoc B Nguyen"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ of California, Los Angeles, Los Angeles, CA","institution_ids":["https://openalex.org/I161318765"]},{"raw_affiliation_string":"Ngoc B Nguyen","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Peng Zhao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peng Zhao","raw_affiliation_strings":["Univ of California, Los Angeles, Los Angeles, CA","Peng Zhao"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ of California, Los Angeles, Los Angeles, CA","institution_ids":["https://openalex.org/I161318765"]},{"raw_affiliation_string":"Peng Zhao","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034513615","display_name":"Suhail Khoja","orcid":"https://orcid.org/0000-0002-7065-9156","observed_orcids":["https://orcid.org/0000-0002-7065-9156"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Suhail Khoja","raw_affiliation_strings":["Univ of California, Los Angeles, Los Angeles, CA","Suhail Khoja"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ of California, Los Angeles, Los Angeles, CA","institution_ids":["https://openalex.org/I161318765"]},{"raw_affiliation_string":"Suhail Khoja","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024903700","display_name":"Rhys J.P. Skelton","orcid":"https://orcid.org/0000-0002-9477-6971","observed_orcids":["https://orcid.org/0000-0002-9477-6971"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Rhys J Skelton","raw_affiliation_strings":["Murdoch Children\u2019s Rsch Institute, The Royal Children\u2019s Hosp, Parkville, Australia","Rhys J Skelton"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Murdoch Children\u2019s Rsch Institute, The Royal Children\u2019s Hosp, Parkville, Australia","institution_ids":[]},{"raw_affiliation_string":"Rhys J Skelton","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102968085","display_name":"Debashis Sahoo","orcid":"https://orcid.org/0000-0003-2329-8228","observed_orcids":["https://orcid.org/0000-0003-2329-8228","https://orcid.org/0000-0001-5494-4099"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Debashis Sahoo","raw_affiliation_strings":["Univ of California, San Diego, La Jolla, CA","Debashis Sahoo"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ of California, San Diego, La Jolla, CA","institution_ids":["https://openalex.org/I36258959"]},{"raw_affiliation_string":"Debashis Sahoo","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074383185","display_name":"David A. Elliott","orcid":"https://orcid.org/0000-0003-1052-7407","observed_orcids":["https://orcid.org/0000-0003-1052-7407"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"David A Elliott","raw_affiliation_strings":["Murdoch Children\u2019s Rsch Institute, The Royal Children\u2019s Hosp, Parkville, Australia","David A Elliott"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Murdoch Children\u2019s Rsch Institute, The Royal Children\u2019s Hosp, Parkville, Australia","institution_ids":[]},{"raw_affiliation_string":"David A Elliott","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019735541","display_name":"Reza Ardehali","orcid":"https://orcid.org/0000-0003-1318-4016","observed_orcids":["https://orcid.org/0000-0003-1318-4016"]},"institutions":[{"id":"https://openalex.org/I161318765","display_name":"University of California, Los Angeles","ror":"https://ror.org/046rm7j60","country_code":"US","type":"education","lineage":["https://openalex.org/I161318765"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Reza Ardehali","raw_affiliation_strings":["Univ of California, Los Angeles, Los Angeles, CA","Reza Ardehali"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ of California, Los Angeles, Los Angeles, CA","institution_ids":["https://openalex.org/I161318765"]},{"raw_affiliation_string":"Reza Ardehali","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"123","issue":"Suppl_1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.8276000022888184,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.8276000022888184,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11677","display_name":"Congenital heart defects research","score":0.16380000114440918,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.004100000020116568,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/human-embryonic-stem-cell-differentiation","display_name":"human embryonic stem cell differentiation","score":0.8980000019073486},{"id":"https://openalex.org/keywords/tbx5","display_name":"TBX5","score":0.7120000123977661},{"id":"https://openalex.org/keywords/nkx2-5","display_name":"Nkx2.5","score":0.9580000042915344},{"id":"https://openalex.org/keywords/cardiomyocyte-isolation","display_name":"cardiomyocyte isolation","score":0.8050000071525574},{"id":"https://openalex.org/keywords/cardiac-progenitor-cells","display_name":"cardiac progenitor cells","score":0.6570000052452087},{"id":"https://openalex.org/keywords/myocardial-infarction","display_name":"myocardial infarction","score":0.8050000071525574},{"id":"https://openalex.org/keywords/cell-therapy","display_name":"cell therapy","score":0.8159999847412109},{"id":"https://openalex.org/keywords/left-ventricle","display_name":"left ventricle","score":0.6510000228881836},{"id":"https://openalex.org/keywords/heart-development","display_name":"heart development","score":0.30300000309944153}],"concepts":[{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.7588392496109009},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.6239357590675354},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5886409282684326},{"id":"https://openalex.org/C2778921608","wikidata":"https://www.wikidata.org/wiki/Q2002035","display_name":"Ventricle","level":2,"score":0.5556879043579102},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5183630585670471},{"id":"https://openalex.org/C62425271","wikidata":"https://www.wikidata.org/wiki/Q14819288","display_name":"Heart development","level":4,"score":0.5173582434654236},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.4962356686592102},{"id":"https://openalex.org/C2777837822","wikidata":"https://www.wikidata.org/wiki/Q14819288","display_name":"Embryonic heart","level":4,"score":0.4517327547073364},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.4268082082271576},{"id":"https://openalex.org/C141035611","wikidata":"https://www.wikidata.org/wiki/Q5532934","display_name":"Genetically modified mouse","level":4,"score":0.4259328246116638},{"id":"https://openalex.org/C2911091166","wikidata":"https://www.wikidata.org/wiki/Q106419912","display_name":"Transplantation","level":2,"score":0.4196995198726654},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3522544503211975},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3157687783241272},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09270572662353516},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.05797263979911804}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1161/res.123.suppl_1.461","doi":"https://doi.org/10.1161/res.123.suppl_1.461","is_oa":false,"landing_page_url":"https://doi.org/10.1161/res.123.suppl_1.461","pdf_url":null,"source":{"id":"https://openalex.org/S10652568","display_name":"Circulation Research","issn_l":"0009-7330","issn":["0009-7330","1524-4571"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Circulation Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9763000011444092}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2981287486","https://openalex.org/W4285198455","https://openalex.org/W4200112962","https://openalex.org/W2018945376","https://openalex.org/W2966375684","https://openalex.org/W3136578409","https://openalex.org/W2148918418","https://openalex.org/W1998839221","https://openalex.org/W7099434099","https://openalex.org/W2171942713"],"abstract_inverted_index":{"Cardiovascular":[0],"disease":[1],"(CVD)":[2],"is":[3,38,54,66],"the":[4,47,51,91,104,167,191,234,270,286,297,310],"leading":[5],"cause":[6],"of":[7,50,93,100,140,158,171,197,204,215,241,272,299,312],"death":[8,41],"worldwide.":[9],"Human":[10],"embryonic":[11],"stem":[12],"cell":[13],"(hESC)":[14],"derived":[15,56,217],"cardiovascular":[16],"progenitor":[17],"cells":[18,80,102,135,153,176,200,221,225],"(CPCs)":[19],"or":[20],"cardiomyocytes":[21],"(CMs)":[22],"represent":[23,136],"a":[24,34,98,122,248],"promising":[25],"candidate":[26],"for":[27,319],"cell-based":[28,320],"therapies":[29,321],"to":[30,46,106,130,147,179,185,212,268,322],"treat":[31,323],"CVD.":[32,324],"Following":[33],"myocardial":[35],"infarction":[36],"there":[37],"extensive":[39],"CM":[40],"and":[42,71,113,120,150,162,181,243,262,274,307],"damage":[43],"primarily":[44],"localized":[45],"left":[48,169],"ventricle":[49,170],"heart,":[52],"which":[53],"predominantly":[55,182],"from":[57,278,317],"first":[58],"heart":[59,76,108,229,313],"field":[60,77,109,230,314],"(FHF)":[61],"CPCs":[62,142,165],"during":[63],"development.":[64],"This":[65],"important":[67],"in":[68,154],"future":[69],"developmental":[70],"regenerative":[72],"therapy":[73],"studies":[74,264,303],"since":[75],"specific":[78,110,124,276,315],"cardiac":[79,101,111,199,224],"will":[81],"be":[82],"desired.":[83],"Current":[84],"hESC":[85,127,251],"differentiation":[86],"protocols":[87],"as":[88,90],"well":[89],"use":[92,298],"cardiac-specific":[94],"reporter":[95,128,252],"lines,":[96],"yield":[97],"mixture":[99],"without":[103,187,296],"ability":[105],"isolate":[107,293],"progenitors":[112],"their":[114],"associated":[115],"progeny.":[116],"Here":[117],"we":[118,246,265,282,291],"generated":[119,247],"characterized":[121],"FHF":[123,141,242,273,294],"TBX5-":[125],"TdTomato/W":[126,255],"line":[129,253],"show":[131,283],"that":[132,143,174,207,226,284],"TBX5":[133,160,163,209,219,254],"+":[134,161],"an":[137,213],"enriched":[138],"population":[139],"can":[144,292],"give":[145],"rise":[146,184],"CMs,":[148,218],"endothelial":[149],"smooth":[151],"muscle":[152],"vitro":[155],".":[156,258],"Transplantation":[157],"hESC-derived":[159,198],"-":[164,220],"into":[166,190],"injured":[168],"mice":[172],"showed":[173],"these":[175],"were":[177,266],"able":[178,267],"survive":[180],"gave":[183],"CMs":[186,232,245,277,295,316],"functional":[188],"integration":[189],"host":[192],"myocardium.":[193],"Furthermore,":[194],"RNA-sequencing":[195],"analysis":[196],"at":[201,233],"different":[202],"stages":[203],"development":[205],"suggested":[206],"while":[208],"expression":[210],"leads":[211],"enrichment":[214],"FHF-":[216],"also":[222],"generate":[223],"resemble":[227],"second":[228],"(SHF)":[231],"transcriptional":[235],"level.":[236],"To":[237],"enable":[238],"prospective":[239],"isolation":[240,271],"SHF":[244,275],"double":[249],"transgenic":[250],"/NKX2-5":[256],"eGFP/W":[257],"Using":[259],"immunostaining,":[260],"qPCR":[261],"electrophysiological":[263],"confirm":[269],"differentiating":[279],"hESCs.":[280],"Finally,":[281],"using":[285],"novel":[287],"surface":[288],"marker,":[289],"CORIN,":[290],"genetic":[300],"reporters.":[301],"Our":[302],"provide":[304],"valuable":[305],"information":[306],"resources":[308],"on":[309],"production":[311],"hESCs":[318]},"counts_by_year":[],"updated_date":"2026-09-24T07:57:37.273135","created_date":"2019-03-11T00:00:00"}