{"id":"https://openalex.org/W2918372158","doi":"https://doi.org/10.1117/12.2509198","title":"In vivo photoacoustic multi-contrast imaging and detection of protein interactions using a small near-infrared photochromic protein","display_name":"In vivo photoacoustic multi-contrast imaging and detection of protein interactions using a small near-infrared photochromic protein","publication_year":2019,"publication_date":"2019-02-27","ids":{"openalex":"https://openalex.org/W2918372158","doi":"https://doi.org/10.1117/12.2509198","mag":"2918372158"},"language":"en","primary_location":{"id":"doi:10.1117/12.2509198","doi":"https://doi.org/10.1117/12.2509198","is_oa":true,"landing_page_url":"https://doi.org/10.1117/12.2509198","pdf_url":"https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10878/1087818/In-vivo-photoacoustic-multi-contrast-imaging-and-detection-of-protein/10.1117/12.2509198.pdf","source":{"id":"https://openalex.org/S4306522477","display_name":"Photons Plus Ultrasound: Imaging and Sensing","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"conference"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Photons Plus Ultrasound: Imaging and Sensing 2019","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10878/1087818/In-vivo-photoacoustic-multi-contrast-imaging-and-detection-of-protein/10.1117/12.2509198.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100440234","display_name":"Lei S. Li","orcid":"https://orcid.org/0000-0001-6164-2646","observed_orcids":["https://orcid.org/0000-0001-6164-2646","https://orcid.org/0000-0001-5304-8380"]},"institutions":[{"id":"https://openalex.org/I122411786","display_name":"California Institute of Technology","ror":"https://ror.org/05dxps055","country_code":"US","type":"education","lineage":["https://openalex.org/I122411786"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lei Li","raw_affiliation_strings":["Caltech (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Caltech (United States)","institution_ids":["https://openalex.org/I122411786"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011375945","display_name":"Anton A. Shemetov","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I129975664","display_name":"Albert Einstein College of Medicine","ror":"https://ror.org/05cf8a891","country_code":"US","type":"education","lineage":["https://openalex.org/I129975664","https://openalex.org/I4210112371"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anton Shemetov","raw_affiliation_strings":["Albert Einstein College of Medicine (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Albert Einstein College of Medicine (United States)","institution_ids":["https://openalex.org/I129975664"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100424725","display_name":"Peng Cheng Hu","orcid":"https://orcid.org/0000-0002-2933-1239","observed_orcids":["https://orcid.org/0000-0002-2933-1239","https://orcid.org/0000-0001-8481-0841"]},"institutions":[{"id":"https://openalex.org/I204465549","display_name":"Washington University in St. Louis","ror":"https://ror.org/01yc7t268","country_code":"US","type":"education","lineage":["https://openalex.org/I204465549"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peng Hu","raw_affiliation_strings":["Washington Univ. in St. Louis (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Washington Univ. in St. Louis (United States)","institution_ids":["https://openalex.org/I204465549"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081184707","display_name":"Daria M. Shcherbakova","orcid":"https://orcid.org/0000-0003-3384-6363","observed_orcids":["https://orcid.org/0000-0003-3384-6363"]},"institutions":[{"id":"https://openalex.org/I129975664","display_name":"Albert Einstein College of Medicine","ror":"https://ror.org/05cf8a891","country_code":"US","type":"education","lineage":["https://openalex.org/I129975664","https://openalex.org/I4210112371"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daria Shcherbakova","raw_affiliation_strings":["Albert Einstein College of Medicine (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Albert Einstein College of Medicine (United States)","institution_ids":["https://openalex.org/I129975664"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049552018","display_name":"Junhui Shi","orcid":"https://orcid.org/0000-0002-5741-2781","observed_orcids":["https://orcid.org/0000-0002-5741-2781"]},"institutions":[{"id":"https://openalex.org/I122411786","display_name":"California Institute of Technology","ror":"https://ror.org/05dxps055","country_code":"US","type":"education","lineage":["https://openalex.org/I122411786"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Junhui Shi","raw_affiliation_strings":["Caltech (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Caltech (United States)","institution_ids":["https://openalex.org/I122411786"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005956622","display_name":"Junjie Yao","orcid":"https://orcid.org/0000-0002-2381-706X","observed_orcids":["https://orcid.org/0000-0002-2381-706X","https://orcid.org/0000-0002-7278-9325"]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Junjie Yao","raw_affiliation_strings":["Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA;","institution_ids":["https://openalex.org/I170897317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033328311","display_name":"Vladislav V. Verkhusha","orcid":"https://orcid.org/0000-0002-2083-8121","observed_orcids":["https://orcid.org/0000-0002-2083-8121"]},"institutions":[{"id":"https://openalex.org/I129975664","display_name":"Albert Einstein College of Medicine","ror":"https://ror.org/05cf8a891","country_code":"US","type":"education","lineage":["https://openalex.org/I129975664","https://openalex.org/I4210112371"]},{"id":"https://openalex.org/I133731052","display_name":"University of Helsinki","ror":"https://ror.org/040af2s02","country_code":"FI","type":"education","lineage":["https://openalex.org/I133731052"]}],"countries":["FI","US"],"is_corresponding":false,"raw_author_name":"Vladislav Verkhusha","raw_affiliation_strings":["Albert Einstein College of Medicine (United States)","Univ. of Helsinki (Finland)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Albert Einstein College of Medicine (United States)","institution_ids":["https://openalex.org/I129975664"]},{"raw_affiliation_string":"Univ. of Helsinki (Finland)","institution_ids":["https://openalex.org/I133731052"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5008233636","display_name":"Lihong V. Wang","orcid":"https://orcid.org/0000-0001-9783-4383","observed_orcids":["https://orcid.org/0000-0001-9783-4383"]},"institutions":[{"id":"https://openalex.org/I122411786","display_name":"California Institute of Technology","ror":"https://ror.org/05dxps055","country_code":"US","type":"education","lineage":["https://openalex.org/I122411786"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lihong V. Wang","raw_affiliation_strings":["Caltech (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Caltech (United States)","institution_ids":["https://openalex.org/I122411786"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.5573,"has_fulltext":true,"cited_by_count":3,"citation_normalized_percentile":{"value":0.78833704,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":94},"biblio":{"volume":"23","issue":null,"first_page":"42","last_page":"42"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12015","display_name":"Photoacoustic and Ultrasonic Imaging","score":0.9692000150680542,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T12015","display_name":"Photoacoustic and Ultrasonic Imaging","score":0.9692000150680542,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.0052999998442828655,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.004900000058114529,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/photoacoustic-computed-tomography","display_name":"photoacoustic computed tomography","score":0.9810000061988831},{"id":"https://openalex.org/keywords/photochromic-proteins","display_name":"photochromic proteins","score":0.7519999742507935},{"id":"https://openalex.org/keywords/near-infrared-probe","display_name":"near-infrared probe","score":0.7200000286102295},{"id":"https://openalex.org/keywords/protein-protein-interaction-detection","display_name":"protein-protein interaction detection","score":0.7749999761581421},{"id":"https://openalex.org/keywords/differential-imaging","display_name":"differential imaging","score":0.4339999854564667},{"id":"https://openalex.org/keywords/deep-tissue-imaging","display_name":"deep tissue imaging","score":0.7099999785423279},{"id":"https://openalex.org/keywords/multi-contrast-imaging","display_name":"multi-contrast imaging","score":0.7009999752044678},{"id":"https://openalex.org/keywords/tumor-imaging","display_name":"tumor imaging","score":0.5009999871253967},{"id":"https://openalex.org/keywords/liver-imaging","display_name":"liver imaging","score":0.5490000247955322},{"id":"https://openalex.org/keywords/photoswitching","display_name":"photoswitching","score":0.6899999976158142}],"concepts":[{"id":"https://openalex.org/C2779073994","wikidata":"https://www.wikidata.org/wiki/Q1751686","display_name":"Pact","level":2,"score":0.8214674592018127},{"id":"https://openalex.org/C81180622","wikidata":"https://www.wikidata.org/wiki/Q899324","display_name":"Photochromism","level":2,"score":0.6849348545074463},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.5098502039909363},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5049124360084534},{"id":"https://openalex.org/C29331040","wikidata":"https://www.wikidata.org/wiki/Q7239554","display_name":"Preclinical imaging","level":3,"score":0.49923062324523926},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4889885187149048},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.4659854769706726},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.358465313911438},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.3006347119808197},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.2859548330307007},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.11097052693367004},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.10645556449890137},{"id":"https://openalex.org/C166957645","wikidata":"https://www.wikidata.org/wiki/Q23498","display_name":"Archaeology","level":1,"score":0.0},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.0},{"id":"https://openalex.org/C95457728","wikidata":"https://www.wikidata.org/wiki/Q309","display_name":"History","level":0,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1117/12.2509198","doi":"https://doi.org/10.1117/12.2509198","is_oa":true,"landing_page_url":"https://doi.org/10.1117/12.2509198","pdf_url":"https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10878/1087818/In-vivo-photoacoustic-multi-contrast-imaging-and-detection-of-protein/10.1117/12.2509198.pdf","source":{"id":"https://openalex.org/S4306522477","display_name":"Photons Plus Ultrasound: Imaging and Sensing","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"conference"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Photons Plus Ultrasound: Imaging and Sensing 2019","raw_type":"proceedings-article"},{"id":"pmh:oai:authors.library.caltech.edu:94561","doi":null,"is_oa":false,"landing_page_url":"https://resolver.caltech.edu/CaltechAUTHORS:20190408-105837786","pdf_url":null,"source":{"id":"https://openalex.org/S4306402161","display_name":"CaltechAUTHORS (California Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I122411786","host_organization_name":"California Institute of Technology","host_organization_lineage":["https://openalex.org/I122411786"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Book Section"}],"best_oa_location":{"id":"doi:10.1117/12.2509198","doi":"https://doi.org/10.1117/12.2509198","is_oa":true,"landing_page_url":"https://doi.org/10.1117/12.2509198","pdf_url":"https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10878/1087818/In-vivo-photoacoustic-multi-contrast-imaging-and-detection-of-protein/10.1117/12.2509198.pdf","source":{"id":"https://openalex.org/S4306522477","display_name":"Photons Plus Ultrasound: Imaging and Sensing","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"conference"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Photons Plus Ultrasound: Imaging and Sensing 2019","raw_type":"proceedings-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2918372158.pdf","grobid_xml":"https://content.openalex.org/works/W2918372158.grobid-xml"},"referenced_works_count":32,"referenced_works":["https://openalex.org/W1686788414","https://openalex.org/W1977871291","https://openalex.org/W1992009035","https://openalex.org/W1997112554","https://openalex.org/W2019189459","https://openalex.org/W2027166852","https://openalex.org/W2036407165","https://openalex.org/W2041353970","https://openalex.org/W2054473817","https://openalex.org/W2086341539","https://openalex.org/W2087750317","https://openalex.org/W2097055018","https://openalex.org/W2119959154","https://openalex.org/W2195567999","https://openalex.org/W2306738577","https://openalex.org/W2478901006","https://openalex.org/W2557701162","https://openalex.org/W2565400716","https://openalex.org/W2593327432","https://openalex.org/W2593823532","https://openalex.org/W2602439357","https://openalex.org/W2606668319","https://openalex.org/W2613485993","https://openalex.org/W2620286813","https://openalex.org/W2669026528","https://openalex.org/W2738524212","https://openalex.org/W2789273102","https://openalex.org/W2801495538","https://openalex.org/W2806174965","https://openalex.org/W2808146670","https://openalex.org/W2894855009","https://openalex.org/W4231576933"],"related_works":["https://openalex.org/W2864616502","https://openalex.org/W2888094576","https://openalex.org/W4395091003","https://openalex.org/W2969175604","https://openalex.org/W2195567999","https://openalex.org/W2306738577","https://openalex.org/W7221690201","https://openalex.org/W4404110248","https://openalex.org/W2314809395","https://openalex.org/W4213434386"],"abstract_inverted_index":{"Photoacoustic":[0],"(PA)":[1],"computed":[2],"tomography":[3],"(PACT)":[4],"is":[5,29,73],"a":[6,60,67,87,147,182,187],"non-invasive":[7,228],"imaging":[8,34],"technique":[9],"offering":[10],"optical":[11,25],"contrast,":[12],"high":[13],"resolution,":[14],"and":[15,49,55,92,125,205,211,222],"deep":[16,172],"penetration":[17],"in":[18,82,123,126,146,151,171,201,214],"biological":[19],"tissues.":[20,173],"PACT,":[21,113],"highly":[22],"sensitive":[23],"to":[24,75],"absorption":[26],"by":[27],"molecules,":[28],"inherently":[30],"suited":[31],"for":[32,134,227],"molecular":[33],"using":[35],"optically":[36],"absorbing":[37],"probes.":[38],"Genetically":[39],"encoded":[40],"probes":[41,109],"with":[42,59,110,153,220],"photochromic":[43,69,108,132],"behavior":[44],"dramatically":[45],"increase":[46],"detection":[47,197],"sensitivity":[48,213],"specificity":[50],"of":[51,178,190,198,218],"PACT":[52,102],"through":[53],"photoswitching":[54,94],"differential":[56,83],"imaging.":[57,232],"Starting":[58],"DrBphP":[61],"bacterial":[62],"phytochrome,":[63],"we":[64,118,165],"have":[65,97,119],"engineered":[66],"near-infrared":[68,131],"probe,":[70],"DrBphP-PCM,":[71,191],"which":[72,104],"superior":[74],"the":[76,106,141,154,161],"full-length":[77],"RpBphP1":[78],"phytochrome":[79],"previously":[80],"used":[81],"PACT.":[84,135],"DrBphP-PCM":[85,121,157,179,221],"has":[86],"smaller":[88],"size,":[89],"better":[90],"folding,":[91],"higher":[93],"contrast.":[95],"We":[96,136],"also":[98],"developed":[99],"an":[100,129],"advanced":[101,130],"technique,":[103],"combines":[105],"reversibly-switchable":[107],"single-impulse":[111],"panoramic":[112],"termed":[114,192],"RS-SIP-PACT.":[115],"Using":[116],"RS-SIP-PACT,":[117],"characterized":[120],"both":[122],"vitro":[124],"vivo":[127],"as":[128],"probe":[133],"introduce":[137],"two":[138],"phytochromes":[139],"into":[140],"same":[142],"mammalian":[143],"cells,":[144],"resulting":[145],"distinctive":[148],"decay":[149,163],"characteristic":[150],"comparison":[152],"cells":[155],"expressing":[156],"only.":[158],"By":[159],"discriminating":[160],"different":[162],"characteristics,":[164],"successfully":[166],"separate":[167],"multiple":[168],"cell":[169],"types":[170],"The":[174,216],"simple":[175],"structural":[176],"organization":[177],"allows":[180],"engineering":[181],"bimolecular":[183],"PA":[184,196],"complementation":[185],"reporter,":[186],"split":[188],"version":[189],"DrSplit.":[193],"DrSplit":[194,223],"enables":[195],"protein-protein":[199],"interactions":[200],"deepseated":[202],"mouse":[203],"tumors":[204],"livers,":[206],"achieving":[207],"125-\u03bcm":[208],"spatial":[209],"resolution":[210],"530-cell":[212],"vivo.":[215],"combination":[217],"RS-SIP-PACT":[219],"holds":[224],"great":[225],"potential":[226],"multi-contrast":[229],"deep-tissue":[230],"functional":[231]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2019-03-11T00:00:00"}