{"id":"https://openalex.org/W2917622374","doi":"https://doi.org/10.1007/s11627-019-09963-9","title":"Improved plant regeneration in callus cultures of Sorghum bicolor (L.) Moench","display_name":"Improved plant regeneration in callus cultures of Sorghum bicolor (L.) Moench","publication_year":2019,"publication_date":"2019-02-26","ids":{"openalex":"https://openalex.org/W2917622374","doi":"https://doi.org/10.1007/s11627-019-09963-9","mag":"2917622374"},"language":"en","primary_location":{"id":"doi:10.1007/s11627-019-09963-9","doi":"https://doi.org/10.1007/s11627-019-09963-9","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s11627-019-09963-9","pdf_url":"https://link.springer.com/content/pdf/10.1007/s11627-019-09963-9.pdf","source":{"id":"https://openalex.org/S200668369","display_name":"In Vitro Cellular & Developmental Biology - Plant","issn_l":"1054-5476","issn":["1054-5476","1475-2689"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","norway-1","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"In Vitro Cellular & Developmental Biology - Plant","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://link.springer.com/content/pdf/10.1007/s11627-019-09963-9.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5030146114","display_name":"Mariola Dreger","orcid":"https://orcid.org/0000-0001-7594-2615","observed_orcids":["https://orcid.org/0000-0001-7594-2615"]},"institutions":[{"id":"https://openalex.org/I4210101068","display_name":"Institute of Natural Fibres and Medicinal Plants","ror":"https://ror.org/019v0z461","country_code":"PL","type":"facility","lineage":["https://openalex.org/I4210101068"]}],"countries":["PL"],"is_corresponding":true,"raw_author_name":"Mariola Dreger","raw_affiliation_strings":["Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630, Poznan, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630, Poznan, Poland","institution_ids":["https://openalex.org/I4210101068"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014656722","display_name":"Rafa\u0142 M\u00f3l","orcid":"https://orcid.org/0000-0002-2748-2690","observed_orcids":["https://orcid.org/0000-0002-2748-2690"]},"institutions":[{"id":"https://openalex.org/I59411706","display_name":"Adam Mickiewicz University in Pozna\u0144","ror":"https://ror.org/04g6bbq64","country_code":"PL","type":"education","lineage":["https://openalex.org/I59411706"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Rafa\u0142 M\u00f3l","raw_affiliation_strings":["Department of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznan, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznan, Poland","institution_ids":["https://openalex.org/I59411706"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036450872","display_name":"Aleksandra Deja","orcid":"https://orcid.org/0000-0001-5290-6128","observed_orcids":["https://orcid.org/0000-0001-5290-6128"]},"institutions":[{"id":"https://openalex.org/I4210101068","display_name":"Institute of Natural Fibres and Medicinal Plants","ror":"https://ror.org/019v0z461","country_code":"PL","type":"facility","lineage":["https://openalex.org/I4210101068"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Aleksandra Deja","raw_affiliation_strings":["Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630, Poznan, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630, Poznan, Poland","institution_ids":["https://openalex.org/I4210101068"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059743765","display_name":"Ewa Raj","orcid":"https://orcid.org/0000-0001-7888-6698","observed_orcids":["https://orcid.org/0000-0001-7888-6698"]},"institutions":[{"id":"https://openalex.org/I59411706","display_name":"Adam Mickiewicz University in Pozna\u0144","ror":"https://ror.org/04g6bbq64","country_code":"PL","type":"education","lineage":["https://openalex.org/I59411706"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Ewa Raj","raw_affiliation_strings":["Department of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznan, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614, Poznan, Poland","institution_ids":["https://openalex.org/I59411706"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066613799","display_name":"G Mankowska","orcid":"https://orcid.org/0000-0002-3725-0156","observed_orcids":["https://orcid.org/0000-0002-3725-0156"]},"institutions":[{"id":"https://openalex.org/I4210101068","display_name":"Institute of Natural Fibres and Medicinal Plants","ror":"https://ror.org/019v0z461","country_code":"PL","type":"facility","lineage":["https://openalex.org/I4210101068"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Gra\u017cyna Ma\u0144kowska","raw_affiliation_strings":["Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630, Poznan, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630, Poznan, Poland","institution_ids":["https://openalex.org/I4210101068"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5006413388","display_name":"Karolina Wielgus","orcid":"https://orcid.org/0000-0003-0629-2991","observed_orcids":["https://orcid.org/0000-0003-0629-2991"]},"institutions":[{"id":"https://openalex.org/I4210101068","display_name":"Institute of Natural Fibres and Medicinal Plants","ror":"https://ror.org/019v0z461","country_code":"PL","type":"facility","lineage":["https://openalex.org/I4210101068"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Karolina Wielgus","raw_affiliation_strings":["Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630, Poznan, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Institute of Natural Fibres and Medicinal Plants, Wojska Polskiego 71b, 60-630, Poznan, Poland","institution_ids":["https://openalex.org/I4210101068"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5030146114"],"corresponding_institution_ids":["https://openalex.org/I4210101068"],"apc_list":{"value":3140,"currency":"USD","value_usd":3140},"apc_paid":{"value":3140,"currency":"USD","value_usd":3140},"fwci":3.6468,"has_fulltext":true,"cited_by_count":31,"citation_normalized_percentile":{"value":0.92277786,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"55","issue":"2","first_page":"190","last_page":"198"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12834","display_name":"Flowering Plant Growth and Cultivation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11247","display_name":"Plant nutrient uptake and metabolism","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sorghum","display_name":"sorghum","score":1.0},{"id":"https://openalex.org/keywords/plant-regeneration","display_name":"plant regeneration","score":0.8659999966621399},{"id":"https://openalex.org/keywords/callus-induction","display_name":"callus induction","score":0.9350000023841858},{"id":"https://openalex.org/keywords/shoot-regeneration","display_name":"shoot regeneration","score":0.9039999842643738},{"id":"https://openalex.org/keywords/honey-supplementation","display_name":"honey supplementation","score":0.7440000176429749},{"id":"https://openalex.org/keywords/sucrose","display_name":"sucrose","score":0.9039999842643738},{"id":"https://openalex.org/keywords/benzyladenine","display_name":"benzyladenine","score":0.8550000190734863},{"id":"https://openalex.org/keywords/explant-browning","display_name":"explant browning","score":0.8169999718666077},{"id":"https://openalex.org/keywords/shoot-tip-explants","display_name":"shoot tip explants","score":0.6100000143051147},{"id":"https://openalex.org/keywords/genetic-transformation","display_name":"genetic transformation","score":0.41999998688697815}],"concepts":[{"id":"https://openalex.org/C9549007","wikidata":"https://www.wikidata.org/wiki/Q1072909","display_name":"Callus","level":2,"score":0.8976848125457764},{"id":"https://openalex.org/C2778597767","wikidata":"https://www.wikidata.org/wiki/Q4027534","display_name":"Sucrose","level":2,"score":0.872136652469635},{"id":"https://openalex.org/C157890192","wikidata":"https://www.wikidata.org/wiki/Q2561978","display_name":"Explant culture","level":3,"score":0.714483916759491},{"id":"https://openalex.org/C21410773","wikidata":"https://www.wikidata.org/wiki/Q220869","display_name":"Shoot","level":2,"score":0.6393541693687439},{"id":"https://openalex.org/C2776970464","wikidata":"https://www.wikidata.org/wiki/Q122043","display_name":"Fructose","level":2,"score":0.6282599568367004},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6019957661628723},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.5732251405715942},{"id":"https://openalex.org/C200806820","wikidata":"https://www.wikidata.org/wiki/Q1798348","display_name":"Murashige and Skoog medium","level":4,"score":0.5214564204216003},{"id":"https://openalex.org/C2780822416","wikidata":"https://www.wikidata.org/wiki/Q1934105","display_name":"Micropropagation","level":4,"score":0.5194477438926697},{"id":"https://openalex.org/C90703135","wikidata":"https://www.wikidata.org/wiki/Q3287622","display_name":"Tissue culture","level":3,"score":0.5130995512008667},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.46504533290863037},{"id":"https://openalex.org/C53007507","wikidata":"https://www.wikidata.org/wiki/Q2760809","display_name":"Browning","level":2,"score":0.4492753744125366},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.24048203229904175},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.15491920709609985},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.08846881985664368}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1007/s11627-019-09963-9","doi":"https://doi.org/10.1007/s11627-019-09963-9","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s11627-019-09963-9","pdf_url":"https://link.springer.com/content/pdf/10.1007/s11627-019-09963-9.pdf","source":{"id":"https://openalex.org/S200668369","display_name":"In Vitro Cellular & Developmental Biology - Plant","issn_l":"1054-5476","issn":["1054-5476","1475-2689"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","norway-1","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"In Vitro Cellular & Developmental Biology - Plant","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1007/s11627-019-09963-9","doi":"https://doi.org/10.1007/s11627-019-09963-9","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s11627-019-09963-9","pdf_url":"https://link.springer.com/content/pdf/10.1007/s11627-019-09963-9.pdf","source":{"id":"https://openalex.org/S200668369","display_name":"In Vitro Cellular & Developmental Biology - Plant","issn_l":"1054-5476","issn":["1054-5476","1475-2689"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","norway-1","poland-40","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"In Vitro Cellular & Developmental Biology - Plant","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.9208999872207642,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320335039","display_name":"Narodowe Centrum Bada\u0144 i Rozwoju","ror":"https://ror.org/05pwfyy15"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2917622374.pdf","grobid_xml":"https://content.openalex.org/works/W2917622374.grobid-xml"},"referenced_works_count":34,"referenced_works":["https://openalex.org/W45934650","https://openalex.org/W1580908855","https://openalex.org/W1738005461","https://openalex.org/W1751115141","https://openalex.org/W1886033354","https://openalex.org/W1909257895","https://openalex.org/W1966670759","https://openalex.org/W1970226220","https://openalex.org/W1985194662","https://openalex.org/W1999013274","https://openalex.org/W2002758643","https://openalex.org/W2003652867","https://openalex.org/W2009471013","https://openalex.org/W2011124715","https://openalex.org/W2024556021","https://openalex.org/W2027061835","https://openalex.org/W2035378526","https://openalex.org/W2045509458","https://openalex.org/W2052871538","https://openalex.org/W2067005926","https://openalex.org/W2069749573","https://openalex.org/W2083886439","https://openalex.org/W2084763131","https://openalex.org/W2113082843","https://openalex.org/W2135572188","https://openalex.org/W2139601143","https://openalex.org/W2155890698","https://openalex.org/W2287373674","https://openalex.org/W2313171757","https://openalex.org/W2322207077","https://openalex.org/W2341376453","https://openalex.org/W2508111331","https://openalex.org/W3015556495","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W7219949847","https://openalex.org/W2169753634","https://openalex.org/W2514505204","https://openalex.org/W2136226520","https://openalex.org/W1864892499","https://openalex.org/W4210861202","https://openalex.org/W2314511236","https://openalex.org/W2206725913","https://openalex.org/W2045170079","https://openalex.org/W2035378526"],"abstract_inverted_index":{"Sorghum":[0],"bicolor":[1,44],"is":[2,16],"a":[3,119,160,202],"recalcitrant":[4],"species":[5],"for":[6,89,98,137,205,257],"tissue":[7,24,30],"culture":[8,170],"regeneration":[9,103,242,259],"and":[10,23,48,56,81,94,97,116,145,199,212,233,251],"genetic":[11],"transformation.":[12],"Browning":[13],"of":[14,18,37,131,175,190,193,220,230,263],"explants":[15],"one":[17],"the":[19,34,65,90,107,141,153,165,179,194,221,225,236,240,247],"factors":[20],"limiting":[21],"organ":[22],"cultures.":[25],"To":[26],"overcome":[27],"this,":[28],"callus":[29,70,85,213,261],"was":[31,87,104,135,207],"initiated":[32],"from":[33],"shoot":[35,258],"tips":[36],"in":[38,58,186,224,235,260],"vitro":[39],"germinating":[40],"seeds":[41],"(":[42],"S.":[43],"cv.":[45],"R\u00f3na":[46],"1),":[47],"then":[49],"cultured":[50],"on":[51,72,106,140],"modified":[52],"MS":[53,108],"media":[54,74],"(Murashige":[55],"Skoog":[57],"Physiol":[59],"Plant":[60],"15:473\u2013497,":[61],"1962":[62],").":[63],"In":[64,164],"first":[66,226],"experiment,":[67,167],"we":[68,168],"tested":[69,105],"induction":[71,86,180,237],"several":[73],"supplemented":[75,113],"with":[76,92,110,114,125,143,159,196],"casein":[77],"hydrolysate,":[78],"polyvinylpyrrolidone,":[79],"honey,":[80],"sucrose.":[82,191],"The":[83,128,210],"best":[84],"recorded":[88],"medium":[91,100,109,142,195,238],"honey":[93,115,176,182,206,234,252],"sucrose":[95,117,126,144,232],"(80.0%)":[96],"control":[99,248],"(79.8%).":[101],"Shoot":[102],"6-benzylaminopurine":[111],"(BAP)":[112],"at":[118,201],"1:1":[120],"ratio":[121],"(by":[122],"weight)":[123],"or":[124,184,188],"only.":[127],"highest":[129],"percentage":[130],"calluses":[132],"regenerating":[133],"shoots":[134],"noted":[136],"those":[138,219],"induced":[139],"honey\u2014approx.":[146],"four":[147],"times":[148],"higher":[149],"when":[150],"compared":[151],"to":[152,172,178,218,244,254],"control.":[154],"Rooted":[155],"plantlets":[156],"were":[157,216],"acclimatized":[158],"92%":[161],"survival":[162,214],"rate.":[163],"second":[166],"analyzed":[169],"responses":[171],"various":[173],"ways":[174],"application":[177],"media:":[181],"(autoclaved":[183],"filtered)":[185],"presence":[187,229],"absence":[189],"Supplementation":[192],"fructose,":[197],"glucose,":[198],"maltose":[200],"proportion":[203],"typical":[204],"also":[208],"investigated.":[209],"explant":[211],"rates":[215],"similar":[217],"honey\u2013sucrose":[222],"combination":[223],"experiment.":[227],"Only":[228],"both":[231],"improved":[239],"total":[241],"rate":[243],"37.9%":[245],"over":[246],"(18.8%).":[249],"Sucrose":[250],"appear":[253],"act":[255],"synergistically":[256],"cultures":[262],"sorghum.":[264]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":8},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":6}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2019-03-02T00:00:00"}