{"id":"https://openalex.org/W291663","doi":null,"title":"Addressing Fatty Acids Toxicity and Production in Biocatalysts","display_name":"Addressing Fatty Acids Toxicity and Production in Biocatalysts","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W291663","mag":"291663"},"language":"en","primary_location":{"id":"pmh:oai:lib.dr.iastate.edu:etd-4557","doi":null,"is_oa":true,"landing_page_url":"https://lib.dr.iastate.edu/etd/13550","pdf_url":"https://lib.dr.iastate.edu/etd/13550","source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://lib.dr.iastate.edu/etd/13550","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101749956","display_name":"Ping Liu","orcid":"https://orcid.org/0000-0003-1091-7333","observed_orcids":["https://orcid.org/0000-0003-1091-7333"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ping Liu","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-1091-7333","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5101749956"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":5.507e-05,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10476","display_name":"Algal biology and biofuel production","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2105","display_name":"Renewable Energy, Sustainability and the Environment"},"field":{"id":"https://openalex.org/fields/21","display_name":"Energy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fatty-acid-toxicity","display_name":"fatty acid toxicity","score":0.9779999852180481},{"id":"https://openalex.org/keywords/octanoic-acid","display_name":"octanoic acid","score":0.9520000219345093},{"id":"https://openalex.org/keywords/hexanoic-acid","display_name":"hexanoic acid","score":0.9359999895095825},{"id":"https://openalex.org/keywords/decanoic-acid","display_name":"capric acid","score":0.9990000128746033},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9210000038146973},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":1.0},{"id":"https://openalex.org/keywords/membrane-integrity","display_name":"membrane integrity","score":0.9580000042915344},{"id":"https://openalex.org/keywords/membrane-leakage","display_name":"membrane leakage","score":0.9860000014305115},{"id":"https://openalex.org/keywords/oleic-acid","display_name":"oleic acid","score":0.8939999938011169},{"id":"https://openalex.org/keywords/acyl-acp-thioesterase","display_name":"acyl-ACP thioesterase","score":0.9070000052452087},{"id":"https://openalex.org/keywords/gene-expression-profiling","display_name":"gene expression profiling","score":0.6909999847412109},{"id":"https://openalex.org/keywords/fatty-acid-production","display_name":"fatty acid production","score":0.5830000042915344},{"id":"https://openalex.org/keywords/biocatalysts","display_name":"biocatalysts","score":0.5770000219345093}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5905476808547974},{"id":"https://openalex.org/C29730261","wikidata":"https://www.wikidata.org/wiki/Q274160","display_name":"Toxicity","level":2,"score":0.5899735689163208},{"id":"https://openalex.org/C2778348673","wikidata":"https://www.wikidata.org/wiki/Q739302","display_name":"Production (economics)","level":2,"score":0.5509131550788879},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.4804755747318268},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.38208088278770447},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.33298957347869873},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.23064175248146057},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.15433767437934875},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.11788228154182434},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.07575517892837524},{"id":"https://openalex.org/C139719470","wikidata":"https://www.wikidata.org/wiki/Q39680","display_name":"Macroeconomics","level":1,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"pmh:oai:lib.dr.iastate.edu:etd-4557","doi":null,"is_oa":true,"landing_page_url":"https://lib.dr.iastate.edu/etd/13550","pdf_url":"https://lib.dr.iastate.edu/etd/13550","source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.847.8823","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.847.8823","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://lib.dr.iastate.edu/cgi/viewcontent.cgi?article%3D4557%26context%3Detd","raw_type":"text"},{"id":"pmh:oai:dr.lib.iastate.edu:20.500.12876/27737","doi":null,"is_oa":false,"landing_page_url":"https://dr.lib.iastate.edu/handle/20.500.12876/27737","pdf_url":null,"source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"archive/lib.dr.iastate.edu/etd/13550/Liu_iastate_0097E_13919.pdf|||Fri Jan 14 19:55:21 UTC 2022","raw_type":"dissertation"},{"id":"mag:291663","doi":null,"is_oa":false,"landing_page_url":"https://lib.dr.iastate.edu/cgi/viewcontent.cgi?article=4557&context=etd","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:lib.dr.iastate.edu:etd-4557","doi":null,"is_oa":true,"landing_page_url":"https://lib.dr.iastate.edu/etd/13550","pdf_url":"https://lib.dr.iastate.edu/etd/13550","source":{"id":"https://openalex.org/S4377196104","display_name":"Iowa State University Digital Repository (Iowa State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I173911158","host_organization_name":"Iowa State University","host_organization_lineage":["https://openalex.org/I173911158"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Graduate Theses and Dissertations","raw_type":"text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6658813440","display_name":"NSF Engineering Reasearch Center for Biorenewable Chemicals (CBiRC)","funder_award_id":"0813570","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320311090","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W291663.pdf","grobid_xml":"https://content.openalex.org/works/W291663.grobid-xml"},"referenced_works_count":222,"referenced_works":["https://openalex.org/W9645576","https://openalex.org/W184956221","https://openalex.org/W201051929","https://openalex.org/W1498617603","https://openalex.org/W1502659707","https://openalex.org/W1557777214","https://openalex.org/W1568352657","https://openalex.org/W1574470162","https://openalex.org/W1578407488","https://openalex.org/W1583901171","https://openalex.org/W1793938508","https://openalex.org/W1884724259","https://openalex.org/W1893995452","https://openalex.org/W1896150578","https://openalex.org/W1906741485","https://openalex.org/W1909424843","https://openalex.org/W1913394811","https://openalex.org/W1963619734","https://openalex.org/W1964717752","https://openalex.org/W1964947381","https://openalex.org/W1965672596","https://openalex.org/W1968592277","https://openalex.org/W1970778844","https://openalex.org/W1975255125","https://openalex.org/W1975537901","https://openalex.org/W1978299684","https://openalex.org/W1978608255","https://openalex.org/W1981267913","https://openalex.org/W1981425843","https://openalex.org/W1982486868","https://openalex.org/W1982771254","https://openalex.org/W1983218387","https://openalex.org/W1986641657","https://openalex.org/W1987005877","https://openalex.org/W1987399778","https://openalex.org/W1987569430","https://openalex.org/W1987643155","https://openalex.org/W1988067659","https://openalex.org/W1988668487","https://openalex.org/W1988908391","https://openalex.org/W1990902952","https://openalex.org/W1991484020","https://openalex.org/W1991490949","https://openalex.org/W1992170087","https://openalex.org/W1993447713","https://openalex.org/W1993562651","https://openalex.org/W1995727065","https://openalex.org/W1998704567","https://openalex.org/W1999397866","https://openalex.org/W2000206290","https://openalex.org/W2000427711","https://openalex.org/W2001449103","https://openalex.org/W2002330593","https://openalex.org/W2004386433","https://openalex.org/W2006061929","https://openalex.org/W2006269956","https://openalex.org/W2007601631","https://openalex.org/W2010803653","https://openalex.org/W2011229731","https://openalex.org/W2011347701","https://openalex.org/W2012259041","https://openalex.org/W2015199121","https://openalex.org/W2015234711","https://openalex.org/W2016343967","https://openalex.org/W2018285405","https://openalex.org/W2022733066","https://openalex.org/W2025028725","https://openalex.org/W2028473199","https://openalex.org/W2028994316","https://openalex.org/W2029453308","https://openalex.org/W2029512487","https://openalex.org/W2030197113","https://openalex.org/W2030269409","https://openalex.org/W2030323381","https://openalex.org/W2031507134","https://openalex.org/W2032216153","https://openalex.org/W2033452660","https://openalex.org/W2034579299","https://openalex.org/W2034645234","https://openalex.org/W2035518327","https://openalex.org/W2037023848","https://openalex.org/W2037744624","https://openalex.org/W2038864049","https://openalex.org/W2039119535","https://openalex.org/W2039880822","https://openalex.org/W2039946383","https://openalex.org/W2041384715","https://openalex.org/W2042491408","https://openalex.org/W2042608260","https://openalex.org/W2043105926","https://openalex.org/W2046697069","https://openalex.org/W2047172734","https://openalex.org/W2049383638","https://openalex.org/W2052081977","https://openalex.org/W2053181423","https://openalex.org/W2055757801","https://openalex.org/W2056717173","https://openalex.org/W2057367867","https://openalex.org/W2058549099","https://openalex.org/W2059738917","https://openalex.org/W2060651626","https://openalex.org/W2062164932","https://openalex.org/W2062734626","https://openalex.org/W2064365613","https://openalex.org/W2065335146","https://openalex.org/W2066469406","https://openalex.org/W2067057812","https://openalex.org/W2067867846","https://openalex.org/W2068019059","https://openalex.org/W2069645361","https://openalex.org/W2071679560","https://openalex.org/W2071762846","https://openalex.org/W2072449699","https://openalex.org/W2073265884","https://openalex.org/W2074893062","https://openalex.org/W2075843142","https://openalex.org/W2076123428","https://openalex.org/W2076375071","https://openalex.org/W2076621797","https://openalex.org/W2077258441","https://openalex.org/W2078864740","https://openalex.org/W2079266069","https://openalex.org/W2082870303","https://openalex.org/W2083512919","https://openalex.org/W2084326894","https://openalex.org/W2084338181","https://openalex.org/W2084529529","https://openalex.org/W2085050171","https://openalex.org/W2086271348","https://openalex.org/W2087126790","https://openalex.org/W2089362778","https://openalex.org/W2091342962","https://openalex.org/W2092488799","https://openalex.org/W2093662846","https://openalex.org/W2094086290","https://openalex.org/W2094651652","https://openalex.org/W2094940364","https://openalex.org/W2095231955","https://openalex.org/W2095386536","https://openalex.org/W2095713308","https://openalex.org/W2096103439","https://openalex.org/W2097994838","https://openalex.org/W2098609634","https://openalex.org/W2099185000","https://openalex.org/W2099321243","https://openalex.org/W2100305481","https://openalex.org/W2102646498","https://openalex.org/W2103017472","https://openalex.org/W2103242178","https://openalex.org/W2103586941","https://openalex.org/W2104112565","https://openalex.org/W2105614583","https://openalex.org/W2106176351","https://openalex.org/W2106945333","https://openalex.org/W2107034410","https://openalex.org/W2107263719","https://openalex.org/W2110384600","https://openalex.org/W2110623616","https://openalex.org/W2110638089","https://openalex.org/W2111481396","https://openalex.org/W2112991502","https://openalex.org/W2113696712","https://openalex.org/W2115214015","https://openalex.org/W2115332881","https://openalex.org/W2116137883","https://openalex.org/W2118301395","https://openalex.org/W2119059442","https://openalex.org/W2119400977","https://openalex.org/W2120014922","https://openalex.org/W2121990877","https://openalex.org/W2122560605","https://openalex.org/W2123949428","https://openalex.org/W2123990415","https://openalex.org/W2126452174","https://openalex.org/W2128282008","https://openalex.org/W2128887055","https://openalex.org/W2129022886","https://openalex.org/W2129527328","https://openalex.org/W2130348414","https://openalex.org/W2134153749","https://openalex.org/W2134293661","https://openalex.org/W2134392026","https://openalex.org/W2136305830","https://openalex.org/W2136876669","https://openalex.org/W2137100180","https://openalex.org/W2142701738","https://openalex.org/W2143960836","https://openalex.org/W2144619571","https://openalex.org/W2144935468","https://openalex.org/W2146503498","https://openalex.org/W2147853672","https://openalex.org/W2149322403","https://openalex.org/W2150893888","https://openalex.org/W2151038079","https://openalex.org/W2151492773","https://openalex.org/W2151618686","https://openalex.org/W2152232041","https://openalex.org/W2152311269","https://openalex.org/W2154900915","https://openalex.org/W2155142785","https://openalex.org/W2155489855","https://openalex.org/W2155747892","https://openalex.org/W2156886382","https://openalex.org/W2157257359","https://openalex.org/W2159022315","https://openalex.org/W2160249997","https://openalex.org/W2160665141","https://openalex.org/W2164176763","https://openalex.org/W2165942233","https://openalex.org/W2166361214","https://openalex.org/W2166606642","https://openalex.org/W2168276180","https://openalex.org/W2168370822","https://openalex.org/W2169251996","https://openalex.org/W2170551349","https://openalex.org/W2170641342","https://openalex.org/W2170939026","https://openalex.org/W2171030481","https://openalex.org/W2171780940","https://openalex.org/W2171948563","https://openalex.org/W2505729989","https://openalex.org/W2511048663"],"related_works":["https://openalex.org/W1982771254","https://openalex.org/W2601537167","https://openalex.org/W2010803653","https://openalex.org/W7095524684","https://openalex.org/W2103586941","https://openalex.org/W7021297373","https://openalex.org/W79284081","https://openalex.org/W2257787929","https://openalex.org/W4392292165","https://openalex.org/W2003285821"],"abstract_inverted_index":{"Fatty":[0],"acids":[1,69,167,174],"recently":[2],"gain":[3],"more":[4,176],"attentions":[5],"due":[6],"to":[7,18,31,88,158,193,240],"its":[8],"potentials":[9],"as":[10,236],"fuel":[11],"and":[12,49,55,72,82,85,130,168,227,253,263],"chemicals.":[13],"However,":[14],"they":[15],"are":[16],"toxic":[17],"biocatalysts":[19],"like":[20],"many":[21],"other":[22],"biofuel":[23],"compounds.":[24],"Understanding":[25],"the":[26,33,40,242],"inhibitory":[27],"mechanism":[28],"can":[29],"help":[30],"improve":[32,169],"production":[34,200],"of":[35,42,67,101,165,179,201,215,221,250],"fatty":[36,45,68,166,205,216,222],"acids.\\nIn":[37],"current":[38],"study,":[39],"toxicity":[41,66,164],"short":[43],"chain":[44,83,171,203],"acids:":[46],"hexanoic,":[47,128],"octanoic,":[48,71,129],"decanoic":[50,73,131],"acid":[51,112,141,217,223],"on":[52,62,80],"Saccharomyces.":[53],"cerevisiae":[54,77,124],"Escherichia.coli":[56],"were":[57],"inverstigated":[58],"with":[59],"a":[60,195,237,248],"focus":[61],"octanoic":[63,140],"acid.":[64],"The":[65,207],"(hexanoic,":[70],"acid)":[74],"in":[75,148,183],"S.":[76,123],"is":[78],"dependent":[79],"doses":[81],"lengths,":[84],"also":[86,137,152],"leads":[87],"damaged":[89],"membrane":[90,102,135,160,264],"intergirity,":[91],"which":[92],"was":[93,151,191,234],"confirmed":[94],"by":[95,108,127],"increased":[96,144],"membreane":[97],"leakage.":[98],"This":[99],"induction":[100],"leakage":[103],"could":[104],"be":[105],"significantly":[106],"decreased":[107],"inctroducing":[109],"endogenous":[110],"oleic":[111],"into":[113],"cell":[114],"membrane.":[115],"Although":[116],"E.coli":[117,197],"cells":[118],"showed":[119],"different":[120],"response":[121],"from":[122,230],"when":[125],"stressed":[126],"acid,":[132],"similar":[133],"disrupted":[134],"intergirity":[136],"occurred":[138],"under":[139],"stress.":[142],"Futhermore,":[143],"saturated:unsaturated":[145],"lipid":[146,157],"ratio":[147],"E.":[149],"coli":[150],"reported.":[153],"Thus,":[154],"manipulating":[155],"memberane":[156],"restore":[159],"integrity":[161],"possibly":[162],"overcome":[163],"production.\\nMedium":[170],"length":[172,204],"carboxylic":[173],"drive":[175],"attention":[177],"because":[178],"their":[180],"broad":[181],"application":[182],"our":[184],"life.":[185],"In":[186],"this":[187],"thesis,":[188],"Transcriptomic":[189],"analysis":[190,246],"applied":[192],"construct":[194],"robust":[196],"strain":[198,211,239],"for":[199],"medium":[202],"acids.":[206],"first":[208],"generation":[209],"engineered":[210],"ML103":[212],"(pXZ18Z)":[213],"(deletion":[214],"\u03b2-oxidation":[218],"pathway,":[219],"over-expression":[220],"elongation":[224],"gene":[225],"(fabZ)":[226],"acyl-ACP":[228],"thioesterase":[229],"R.":[231],"communis":[232],")":[233],"used":[235],"host":[238],"study":[241],"possible":[243],"pathway.":[244],"Transcriptome":[245],"revealed":[247],"series":[249],"disturbed":[251],"genes":[252],"transcription":[254],"factors":[255],"including":[256],"stress":[257],"response,":[258],"biofilm,":[259],"transporters,":[260],"nitrogen":[261],"limitation":[262],"disruption.":[265]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
