{"id":"https://openalex.org/W2915479218","doi":"https://doi.org/10.21597/jist.446068","title":"Knockdown of TIGAR Induces Apoptosis and Autophagy with Modulates NF-\u03baB and HO-1 Expression in A549 Lung Cancer Cells","display_name":"Knockdown of TIGAR Induces Apoptosis and Autophagy with Modulates NF-\u03baB and HO-1 Expression in A549 Lung Cancer Cells","publication_year":2019,"publication_date":"2019-02-18","ids":{"openalex":"https://openalex.org/W2915479218","doi":"https://doi.org/10.21597/jist.446068","mag":"2915479218"},"language":"en","primary_location":{"id":"doi:10.21597/jist.446068","doi":"https://doi.org/10.21597/jist.446068","is_oa":true,"landing_page_url":"https://doi.org/10.21597/jist.446068","pdf_url":"https://dergipark.org.tr/en/download/article-file/651579","source":{"id":"https://openalex.org/S4387288959","display_name":"I\u011fd\u0131r \u00dcniversitesi Fen Bilimleri Enstit\u00fcs\u00fc Dergisi","issn_l":"2536-4618","issn":["2536-4618"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["tr-dizin"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"I\u011fd\u0131r \u00dcniversitesi Fen Bilimleri Enstit\u00fcs\u00fc Dergisi","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://dergipark.org.tr/en/download/article-file/651579","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5066397483","display_name":"Can Ali A\u011fca","orcid":"https://orcid.org/0000-0002-0244-3767","observed_orcids":["https://orcid.org/0000-0002-0244-3767","https://orcid.org/0000-0003-1969-1182"]},"institutions":[{"id":"https://openalex.org/I147699246","display_name":"Bing\u00f6l University","ror":"https://ror.org/03hx84x94","country_code":"TR","type":"education","lineage":["https://openalex.org/I147699246"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Can Ali A\u011fca","raw_affiliation_strings":["B\u0130NG\u00d6L \u00dcN\u0130VERS\u0130TES\u0130"],"raw_orcid":"https://orcid.org/0000-0002-0244-3767","affiliations":[{"raw_affiliation_string":"B\u0130NG\u00d6L \u00dcN\u0130VERS\u0130TES\u0130","institution_ids":["https://openalex.org/I147699246"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5017328096","display_name":"Osama Hamid Shareef","orcid":"https://orcid.org/0000-0001-6859-4317","observed_orcids":["https://orcid.org/0000-0001-6859-4317"]},"institutions":[{"id":"https://openalex.org/I147699246","display_name":"Bing\u00f6l University","ror":"https://ror.org/03hx84x94","country_code":"TR","type":"education","lineage":["https://openalex.org/I147699246"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Osama Hamid Shareef","raw_affiliation_strings":["B\u0130NG\u00d6L \u00dcN\u0130VERS\u0130TES\u0130"],"raw_orcid":"https://orcid.org/0000-0001-6859-4317","affiliations":[{"raw_affiliation_string":"B\u0130NG\u00d6L \u00dcN\u0130VERS\u0130TES\u0130","institution_ids":["https://openalex.org/I147699246"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I147699246"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00975482,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"9","issue":"1","first_page":"310","last_page":"320"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10631","display_name":"Cancer, Hypoxia, and Metabolism","score":0.57669997215271,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10631","display_name":"Cancer, Hypoxia, and Metabolism","score":0.57669997215271,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.13850000500679016,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10587","display_name":"Autophagy in Disease and Therapy","score":0.1071000024676323,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tigar","display_name":"TIGAR","score":0.9990000128746033},{"id":"https://openalex.org/keywords/lung-cancer","display_name":"lung cancer","score":0.9079999923706055},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.8510000109672546},{"id":"https://openalex.org/keywords/autophagy","display_name":"autophagy","score":1.0},{"id":"https://openalex.org/keywords/nf-kappab","display_name":"NF-\u03baB","score":0.9610000252723694},{"id":"https://openalex.org/keywords/heme-oxygenase-1","display_name":"heme oxygenase-1","score":1.0},{"id":"https://openalex.org/keywords/reactive-oxygen-species","display_name":"reactive oxygen species","score":0.9990000128746033},{"id":"https://openalex.org/keywords/nadph","display_name":"NADPH","score":0.9950000047683716},{"id":"https://openalex.org/keywords/glutathione","display_name":"glutathione","score":0.6919999718666077},{"id":"https://openalex.org/keywords/fructose-2-6-bisphosphate","display_name":"fructose-2,6-bisphosphate","score":0.9340000152587891},{"id":"https://openalex.org/keywords/glucose-metabolism","display_name":"glucose metabolism","score":0.8370000123977661},{"id":"https://openalex.org/keywords/a549-cells","display_name":"A549 cells","score":0.9290000200271606},{"id":"https://openalex.org/keywords/sirna-knockdown","display_name":"siRNA knockdown","score":0.7940000295639038},{"id":"https://openalex.org/keywords/cell-viability","display_name":"cell viability","score":0.5960000157356262},{"id":"https://openalex.org/keywords/colony-formation","display_name":"colony formation","score":0.8190000057220459}],"concepts":[{"id":"https://openalex.org/C173396325","wikidata":"https://www.wikidata.org/wiki/Q1501275","display_name":"Gene knockdown","level":3,"score":0.8488131761550903},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.6002295613288879},{"id":"https://openalex.org/C81729549","wikidata":"https://www.wikidata.org/wiki/Q4649475","display_name":"A549 cell","level":3,"score":0.5851793885231018},{"id":"https://openalex.org/C203522944","wikidata":"https://www.wikidata.org/wiki/Q288322","display_name":"Autophagy","level":3,"score":0.5404151082038879},{"id":"https://openalex.org/C48349386","wikidata":"https://www.wikidata.org/wiki/Q424361","display_name":"Reactive oxygen species","level":2,"score":0.5185416340827942},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.4672076106071472},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4670841693878174},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.46156826615333557},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4211491048336029},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3848028779029846},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18378812074661255}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.21597/jist.446068","doi":"https://doi.org/10.21597/jist.446068","is_oa":true,"landing_page_url":"https://doi.org/10.21597/jist.446068","pdf_url":"https://dergipark.org.tr/en/download/article-file/651579","source":{"id":"https://openalex.org/S4387288959","display_name":"I\u011fd\u0131r \u00dcniversitesi Fen Bilimleri Enstit\u00fcs\u00fc Dergisi","issn_l":"2536-4618","issn":["2536-4618"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["tr-dizin"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"I\u011fd\u0131r \u00dcniversitesi Fen Bilimleri Enstit\u00fcs\u00fc Dergisi","raw_type":"journal-article"},{"id":"pmh:oai:dergipark.gov.tr:article/446068","doi":null,"is_oa":false,"landing_page_url":"http://dergipark.gov.tr/jist/issue/43413/446068","pdf_url":null,"source":{"id":"https://openalex.org/S4387288959","display_name":"I\u011fd\u0131r \u00dcniversitesi Fen Bilimleri Enstit\u00fcs\u00fc Dergisi","issn_l":"2536-4618","issn":["2536-4618"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["tr-dizin"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"\\n Volume: 9, Issue: 1\\n 310-320\\n \\n","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:dergipark.org.tr:article/446068","doi":null,"is_oa":false,"landing_page_url":"https://dergipark.org.tr/tr/pub/jist/issue/43413/446068","pdf_url":null,"source":{"id":"https://openalex.org/S4387288959","display_name":"I\u011fd\u0131r \u00dcniversitesi Fen Bilimleri Enstit\u00fcs\u00fc Dergisi","issn_l":"2536-4618","issn":["2536-4618"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["tr-dizin"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.21597/jist.446068","doi":"https://doi.org/10.21597/jist.446068","is_oa":true,"landing_page_url":"https://doi.org/10.21597/jist.446068","pdf_url":"https://dergipark.org.tr/en/download/article-file/651579","source":{"id":"https://openalex.org/S4387288959","display_name":"I\u011fd\u0131r \u00dcniversitesi Fen Bilimleri Enstit\u00fcs\u00fc Dergisi","issn_l":"2536-4618","issn":["2536-4618"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["tr-dizin"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"I\u011fd\u0131r \u00dcniversitesi Fen Bilimleri Enstit\u00fcs\u00fc Dergisi","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.979200005531311,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8952949849","display_name":"Akci\u011fer Kanseri Tedavisinde TIGAR Proteinin Rol\u00fcn\u00fcn De\u015fifre Edilmesi","funder_award_id":"215S192","funder_id":"https://openalex.org/F4320322626","funder_display_name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ft\u0131rma Kurumu"}],"funders":[{"id":"https://openalex.org/F4320322626","display_name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ft\u0131rma Kurumu","ror":"https://ror.org/04w9kkr77"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2915479218.pdf","grobid_xml":"https://content.openalex.org/works/W2915479218.grobid-xml"},"referenced_works_count":35,"referenced_works":["https://openalex.org/W1595404534","https://openalex.org/W1967291007","https://openalex.org/W1977918575","https://openalex.org/W1977930150","https://openalex.org/W1982174124","https://openalex.org/W1984543978","https://openalex.org/W1986391901","https://openalex.org/W2011608072","https://openalex.org/W2013747211","https://openalex.org/W2016468663","https://openalex.org/W2026117909","https://openalex.org/W2039220817","https://openalex.org/W2046595267","https://openalex.org/W2064039900","https://openalex.org/W2066371867","https://openalex.org/W2070897231","https://openalex.org/W2072549244","https://openalex.org/W2073819646","https://openalex.org/W2082221119","https://openalex.org/W2084085176","https://openalex.org/W2086667809","https://openalex.org/W2104471136","https://openalex.org/W2107277218","https://openalex.org/W2116240218","https://openalex.org/W2117840705","https://openalex.org/W2117960515","https://openalex.org/W2119239003","https://openalex.org/W2160295920","https://openalex.org/W2163409417","https://openalex.org/W2163981813","https://openalex.org/W2200781451","https://openalex.org/W2555410456","https://openalex.org/W2605255264","https://openalex.org/W2607465958","https://openalex.org/W2614646550"],"related_works":["https://openalex.org/W2070897231","https://openalex.org/W2046595267","https://openalex.org/W2013747211","https://openalex.org/W2803534504","https://openalex.org/W4211152727","https://openalex.org/W1016565172","https://openalex.org/W4408784659","https://openalex.org/W4391581756","https://openalex.org/W3125120921","https://openalex.org/W4220703491"],"abstract_inverted_index":{"The":[0,35],"tp53-induced":[1],"glycolysis":[2],"and":[3,18,44,79,86,98,115,123,135,150,159,184,188,191],"apoptosis":[4,43,134,183],"regulator":[5],"(TIGAR)":[6],"protein":[7,97],"controls":[8],"fructose-2,":[9],"6-":[10],"bisphosphate":[11],"(Fru-2,":[12],"6-P2)":[13],"levels":[14,26,158,162],"during":[15],"glucose":[16],"metabolism":[17],"helps":[19],"maintain":[20],"nicotinamide":[21],"adenine":[22],"dinucleotide":[23],"phosphate":[24],"(NADPH)":[25],"to":[27,40,95,181],"recycle":[28],"glutathione":[29],"(GSH),":[30],"a":[31,196],"key":[32],"intracellular":[33],"antioxidant.":[34],"present":[36],"study":[37,166],"was":[38],"designed":[39],"investigate":[41],"the":[42,57,63,171,200],"autophagy":[45,185],"mechanisms":[46],"via":[47],"reactive":[48],"oxygen":[49],"species":[50],"(ROS)":[51],"that":[52,117,130],"underlie":[53],"TIGAR":[54,104,120,131,175,194],"knockdown":[55,105,132,176],"in":[56,106,177],"A549":[58,68,107],"cell":[59,74,108],"line.":[60],"To":[61],"detect":[62],"influence":[64],"of":[65,119,141,145,170,173,202],"siRNA-TIGAR":[66],"on":[67],"lung":[69,178,203],"cancer":[70,179],"cells,":[71],"we":[72],"performed":[73],"viabilty,":[75],"colony":[76,125],"formation,":[77],"ROS,":[78],"NADPH":[80,161],"assays.":[81],"In":[82],"addition,":[83],"Western":[84],"blotting":[85],"real-time":[87],"polymerase":[88],"chain":[89],"reaction":[90],"(PCR)":[91],"assays":[92],"were":[93,113,163],"used":[94],"measure":[96],"mRNA":[99],"expression":[100,190],"levels,":[101],"respectively.":[102],"After":[103],"lines,":[109],"various":[110],"assay":[111],"parameters":[112],"analyzed":[114],"showed":[116],"down-regulation":[118],"inhibited":[121],"viability":[122],"decreased":[124,160],"formation.":[126],"We":[127],"also":[128],"demonstrated":[129],"induced":[133],"autophagy,":[136],"followed":[137],"by":[138],"an":[139],"induction":[140],"nuclear":[142],"factor":[143],"kappa-light-chain-enhancer":[144],"activated":[146],"B":[147],"cells":[148,180],"(NF-\u03baB)":[149],"heme":[151],"oxygenase-1":[152],"(HO-1)":[153],"expression.":[154],"Furthermore,":[155],"increased":[156],"ROS":[157],"observed.":[164],"This":[165],"supports":[167],"our":[168],"understanding":[169],"possibility":[172],"employing":[174],"enhance":[182],"with":[186],"NF-\u03baB":[187],"HO-1":[189],"then":[192],"suggest":[193],"as":[195],"potential":[197],"target":[198],"for":[199],"treatment":[201],"cancer.":[204]},"counts_by_year":[],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2019-03-02T00:00:00"}