{"id":"https://openalex.org/W2915058713","doi":"https://doi.org/10.3791/59143","title":"Extraction of Extracellular Vesicles from Whole Tissue","display_name":"Extraction of Extracellular Vesicles from Whole Tissue","publication_year":2019,"publication_date":"2019-02-07","ids":{"openalex":"https://openalex.org/W2915058713","doi":"https://doi.org/10.3791/59143","mag":"2915058713","pmid":"https://pubmed.ncbi.nlm.nih.gov/30799860","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/7098067"},"language":"en","primary_location":{"id":"doi:10.3791/59143","doi":"https://doi.org/10.3791/59143","is_oa":false,"landing_page_url":"https://doi.org/10.3791/59143","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7098067","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063965540","display_name":"Stephanie N. Hurwitz","orcid":"https://orcid.org/0000-0002-6975-9168","observed_orcids":["https://orcid.org/0000-0002-6975-9168"]},"institutions":[{"id":"https://openalex.org/I103163165","display_name":"Florida State University","ror":"https://ror.org/05g3dte14","country_code":"US","type":"education","lineage":["https://openalex.org/I103163165"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephanie  N. Hurwitz","raw_affiliation_strings":["Department of Biomedical Sciences, Florida State University College of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences, Florida State University College of Medicine","institution_ids":["https://openalex.org/I103163165"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086078445","display_name":"James Olcese","orcid":"https://orcid.org/0000-0002-9980-330X","observed_orcids":["https://orcid.org/0000-0002-9980-330X"]},"institutions":[{"id":"https://openalex.org/I103163165","display_name":"Florida State University","ror":"https://ror.org/05g3dte14","country_code":"US","type":"education","lineage":["https://openalex.org/I103163165"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James  M. Olcese","raw_affiliation_strings":["Department of Biomedical Sciences, Florida State University College of Medicine; james.olcese@med.fsu.edu","Department of Biomedical Sciences, Florida State University College of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences, Florida State University College of Medicine; james.olcese@med.fsu.edu","institution_ids":["https://openalex.org/I103163165"]},{"raw_affiliation_string":"Department of Biomedical Sciences, Florida State University College of Medicine","institution_ids":["https://openalex.org/I103163165"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088547350","display_name":"David G. Meckes","orcid":"https://orcid.org/0000-0002-0836-2108","observed_orcids":["https://orcid.org/0000-0002-0836-2108"]},"institutions":[{"id":"https://openalex.org/I103163165","display_name":"Florida State University","ror":"https://ror.org/05g3dte14","country_code":"US","type":"education","lineage":["https://openalex.org/I103163165"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David G. Meckes Jr.","raw_affiliation_strings":["Department of Biomedical Sciences, Florida State University College of Medicine; david.meckes@med.fsu.edu","Department of Biomedical Sciences, Florida State University College of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Sciences, Florida State University College of Medicine; david.meckes@med.fsu.edu","institution_ids":["https://openalex.org/I103163165"]},{"raw_affiliation_string":"Department of Biomedical Sciences, Florida State University College of Medicine","institution_ids":["https://openalex.org/I103163165"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I103163165"],"apc_list":null,"apc_paid":null,"fwci":1.7845,"has_fulltext":false,"cited_by_count":60,"citation_normalized_percentile":{"value":0.85190693,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":96,"max":99},"biblio":{"volume":null,"issue":"144","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12793","display_name":"Caveolin-1 and cellular processes","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/extracellular-vesicles","display_name":"extracellular vesicles","score":1.0},{"id":"https://openalex.org/keywords/small-vesicles","display_name":"small vesicles","score":0.6430000066757202},{"id":"https://openalex.org/keywords/brain-tissue","display_name":"brain tissue","score":0.796999990940094},{"id":"https://openalex.org/keywords/tumor-tissue","display_name":"tumor tissue","score":0.9900000095367432},{"id":"https://openalex.org/keywords/vesicle-isolation","display_name":"vesicle isolation","score":0.6050000190734863},{"id":"https://openalex.org/keywords/vesicle-characterization","display_name":"vesicle characterization","score":0.7649999856948853},{"id":"https://openalex.org/keywords/biomarkers","display_name":"biomarkers","score":0.4390000104904175},{"id":"https://openalex.org/keywords/neurodegeneration","display_name":"neurodegeneration","score":0.7960000038146973},{"id":"https://openalex.org/keywords/cancer","display_name":"cancer","score":0.8880000114440918}],"concepts":[{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.7388198375701904},{"id":"https://openalex.org/C2992929900","wikidata":"https://www.wikidata.org/wiki/Q21097485","display_name":"Extracellular vesicles","level":2,"score":0.7322983741760254},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5424507260322571},{"id":"https://openalex.org/C2781197716","wikidata":"https://www.wikidata.org/wiki/Q864574","display_name":"Biomarker","level":2,"score":0.5123051404953003},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.47346487641334534},{"id":"https://openalex.org/C26291073","wikidata":"https://www.wikidata.org/wiki/Q310484","display_name":"Ex vivo","level":3,"score":0.46857428550720215},{"id":"https://openalex.org/C2908689518","wikidata":"https://www.wikidata.org/wiki/Q21097485","display_name":"Extracellular vesicle","level":5,"score":0.4649544954299927},{"id":"https://openalex.org/C2776925932","wikidata":"https://www.wikidata.org/wiki/Q1755122","display_name":"Neurodegeneration","level":3,"score":0.43793898820877075},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.41001462936401367},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4022047817707062},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.2721903920173645},{"id":"https://openalex.org/C20518536","wikidata":"https://www.wikidata.org/wiki/Q6840469","display_name":"Microvesicles","level":4,"score":0.24904319643974304},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19190895557403564},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.18043869733810425},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.179460346698761},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.1398569941520691},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.11863213777542114},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.07335877418518066},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000067128","descriptor_name":"Extracellular Vesicles","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001921","descriptor_name":"Brain","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008854","descriptor_name":"Microscopy, Electron","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.3791/59143","doi":"https://doi.org/10.3791/59143","is_oa":false,"landing_page_url":"https://doi.org/10.3791/59143","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"doi:10.3791/59143-v","doi":"https://doi.org/10.3791/59143-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/59143-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmid:30799860","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30799860","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:7098067","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7098067","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Vis Exp","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:7098067","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7098067","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Vis Exp","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8371999859809875}],"study_designs":[],"awards":[{"id":"https://openalex.org/G374053539","display_name":"Modulation of Host Cell Exosome Content and Function by EBV LMP1","funder_award_id":"R01CA204621","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320309170","display_name":"Florida State University","ror":"https://ror.org/05g3dte14"},{"id":"https://openalex.org/F4320316829","display_name":"Florida Agricultural and Mechanical University","ror":"https://ror.org/00c4wc133"},{"id":"https://openalex.org/F4320332167","display_name":"Directorate for Biological Sciences","ror":"https://ror.org/001xhss06"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W1535490554","https://openalex.org/W1566386955","https://openalex.org/W1601681275","https://openalex.org/W1762620449","https://openalex.org/W1950118853","https://openalex.org/W1974954833","https://openalex.org/W1981820123","https://openalex.org/W1991390602","https://openalex.org/W2011537287","https://openalex.org/W2030701238","https://openalex.org/W2069437848","https://openalex.org/W2149612630","https://openalex.org/W2149806687","https://openalex.org/W2150904674","https://openalex.org/W2155366992","https://openalex.org/W2159401023","https://openalex.org/W2169977305","https://openalex.org/W2255949496","https://openalex.org/W2259481044","https://openalex.org/W2338671470","https://openalex.org/W2399281560","https://openalex.org/W2418315808","https://openalex.org/W2466274209","https://openalex.org/W2527247503","https://openalex.org/W2555091598","https://openalex.org/W2569150814","https://openalex.org/W2739380147","https://openalex.org/W2748470793","https://openalex.org/W2782295218","https://openalex.org/W2790701366","https://openalex.org/W2793326518","https://openalex.org/W2805630246","https://openalex.org/W2807776100","https://openalex.org/W4230882199"],"related_works":["https://openalex.org/W4307973158","https://openalex.org/W4412801346","https://openalex.org/W4386019768","https://openalex.org/W2125451833","https://openalex.org/W2560660074","https://openalex.org/W3124334218","https://openalex.org/W3165569042","https://openalex.org/W4361268660","https://openalex.org/W4292196699","https://openalex.org/W4407901213"],"abstract_inverted_index":{"Circulating":[0],"and":[1,21,49,69,74,79,119,139],"interstitial":[2],"small":[3,107],"membrane-bound":[4],"extracellular":[5],"vesicles":[6,44,81],"(EVs)":[7],"represent":[8],"promising":[9],"targets":[10],"for":[11,87,105,122,132],"the":[12,28,41,57,62,127],"development":[13],"of":[14,30,34,43,59,64,89,109,129,142,150],"novel":[15],"diagnostic":[16],"or":[17,114],"prognostic":[18],"biomarker":[19],"assays,":[20],"likely":[22],"serve":[23],"as":[24,144,146],"important":[25],"players":[26],"in":[27,52,61,83],"progression":[29],"a":[31,102],"vast":[32],"spectrum":[33],"diseases.":[35],"Current":[36],"research":[37],"is":[38],"focused":[39],"on":[40],"characterization":[42,141],"secreted":[45],"from":[46,91,111],"multiple":[47,133],"cell":[48],"tissue":[50,93],"types":[51],"order":[53],"to":[54,77],"better":[55],"understand":[56],"role":[58],"EVs":[60,90,108],"pathogenesis":[63],"conditions":[65],"including":[66,117,136],"neurodegeneration,":[67],"inflammation,":[68],"cancer.":[70],"However,":[71],"globally":[72],"consistent":[73],"reproducible":[75],"techniques":[76],"isolate":[78],"purify":[80],"remain":[82],"progress.":[84],"Moreover,":[85],"methods":[86],"extraction":[88],"solid":[92],"ex":[94],"vivo":[95],"are":[96],"scarcely":[97],"described.":[98],"Here,":[99],"we":[100],"provide":[101],"detailed":[103],"protocol":[104],"extracting":[106],"interest":[110],"whole":[112],"fresh":[113],"frozen":[115],"tissues,":[116],"brain":[118],"tumor":[120],"specimens,":[121],"further":[123],"characterization.":[124],"We":[125],"demonstrate":[126],"adaptability":[128],"this":[130],"method":[131],"downstream":[134],"analyses,":[135],"electron":[137],"microscopy":[138],"immunophenotypic":[140],"vesicles,":[143],"well":[145],"quantitative":[147],"mass":[148],"spectrometry":[149],"EV":[151],"proteins.":[152]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":13},{"year":2024,"cited_by_count":8},{"year":2023,"cited_by_count":10},{"year":2022,"cited_by_count":12},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2019-02-21T00:00:00"}
