{"id":"https://openalex.org/W2914808253","doi":"https://doi.org/10.1101/537589","title":"A Moving Target: The Megaplasmid pMPPla107 Sensitizes Cells to an Inhibitory Agent Conserved Across Pseudomonas spp","display_name":"A Moving Target: The Megaplasmid pMPPla107 Sensitizes Cells to an Inhibitory Agent Conserved Across Pseudomonas spp","publication_year":2019,"publication_date":"2019-01-31","ids":{"openalex":"https://openalex.org/W2914808253","doi":"https://doi.org/10.1101/537589","mag":"2914808253"},"language":"en","primary_location":{"id":"doi:10.1101/537589","doi":"https://doi.org/10.1101/537589","is_oa":true,"landing_page_url":"https://doi.org/10.1101/537589","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2019/01/31/537589.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2019/01/31/537589.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058730002","display_name":"Brian A. Smith","orcid":"https://orcid.org/0000-0002-8458-0764","observed_orcids":["https://orcid.org/0000-0002-8458-0764"]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brian A. Smith","raw_affiliation_strings":["School of Plant Sciences, University of Arizona, Tucson, AZ, USA"],"raw_orcid":"https://orcid.org/0000-0002-8458-0764","affiliations":[{"raw_affiliation_string":"School of Plant Sciences, University of Arizona, Tucson, AZ, USA","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048464705","display_name":"Yelena Feinstein","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yelena Feinstein","raw_affiliation_strings":["Analytical and Biological Mass Spectrometry Core, University of Arizona, Tucson, AZ, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Analytical and Biological Mass Spectrometry Core, University of Arizona, Tucson, AZ, USA","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087184284","display_name":"Meara Clark","orcid":"https://orcid.org/0000-0001-5020-4675","observed_orcids":["https://orcid.org/0000-0001-5020-4675"]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Meara Clark","raw_affiliation_strings":["School of Plant Sciences, University of Arizona, Tucson, AZ, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Plant Sciences, University of Arizona, Tucson, AZ, USA","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067145615","display_name":"David A. Baltrus","orcid":"https://orcid.org/0000-0002-5166-9551","observed_orcids":["https://orcid.org/0000-0002-5166-9551"]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"David A. Baltrus","raw_affiliation_strings":["School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ, USA","School of Plant Sciences, University of Arizona, Tucson, AZ, USA"],"raw_orcid":"https://orcid.org/0000-0002-5166-9551","affiliations":[{"raw_affiliation_string":"School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ, USA","institution_ids":["https://openalex.org/I138006243"]},{"raw_affiliation_string":"School of Plant Sciences, University of Arizona, Tucson, AZ, USA","institution_ids":["https://openalex.org/I138006243"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5067145615"],"corresponding_institution_ids":["https://openalex.org/I138006243"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.49410000443458557,"subfield":{"id":"https://openalex.org/subfields/1313","display_name":"Molecular Medicine"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.49410000443458557,"subfield":{"id":"https://openalex.org/subfields/1313","display_name":"Molecular Medicine"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.42750000953674316,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10593","display_name":"Bacterial biofilms and quorum sensing","score":0.026399999856948853,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pseudomonas-syringae","display_name":"Pseudomonas syringae","score":0.8960000276565552},{"id":"https://openalex.org/keywords/plasmid-acquisition","display_name":"plasmid acquisition","score":0.9779999852180481},{"id":"https://openalex.org/keywords/collateral-sensitivity","display_name":"collateral sensitivity","score":0.9789999723434448},{"id":"https://openalex.org/keywords/horizontal-gene-transfer","display_name":"horizontal gene transfer","score":0.9229999780654907},{"id":"https://openalex.org/keywords/antibiotic-resistance-genes","display_name":"antibiotic resistance genes","score":0.7599999904632568}],"concepts":[{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.8488998413085938},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7310311794281006},{"id":"https://openalex.org/C92938381","wikidata":"https://www.wikidata.org/wiki/Q83185","display_name":"Horizontal gene transfer","level":4,"score":0.7247982025146484},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5854918956756592},{"id":"https://openalex.org/C132533263","wikidata":"https://www.wikidata.org/wiki/Q2347362","display_name":"Mobile genetic elements","level":4,"score":0.5644480586051941},{"id":"https://openalex.org/C2781463711","wikidata":"https://www.wikidata.org/wiki/Q131143","display_name":"Pseudomonas","level":3,"score":0.5519053339958191},{"id":"https://openalex.org/C94665300","wikidata":"https://www.wikidata.org/wiki/Q63391344","display_name":"Antibiotic resistance","level":3,"score":0.5254320502281189},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.502474308013916},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4741216003894806},{"id":"https://openalex.org/C4937899","wikidata":"https://www.wikidata.org/wiki/Q68541106","display_name":"Antimicrobial","level":2,"score":0.4504127502441406},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.41593849658966064},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.41017451882362366},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.36845895648002625},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.23005816340446472}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1101/537589","doi":"https://doi.org/10.1101/537589","is_oa":true,"landing_page_url":"https://doi.org/10.1101/537589","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2019/01/31/537589.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.1101/537589","doi":"https://doi.org/10.1101/537589","is_oa":true,"landing_page_url":"https://doi.org/10.1101/537589","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2019/01/31/537589.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.4000000059604645}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2914808253.pdf","grobid_xml":"https://content.openalex.org/works/W2914808253.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W1057466098","https://openalex.org/W1493400288","https://openalex.org/W1544835748","https://openalex.org/W1548925052","https://openalex.org/W1858510480","https://openalex.org/W1943112463","https://openalex.org/W1968305507","https://openalex.org/W1974014862","https://openalex.org/W1978439945","https://openalex.org/W1983455597","https://openalex.org/W1997221625","https://openalex.org/W2015483321","https://openalex.org/W2040539560","https://openalex.org/W2041949408","https://openalex.org/W2061878956","https://openalex.org/W2066021029","https://openalex.org/W2066396905","https://openalex.org/W2075287054","https://openalex.org/W2081418090","https://openalex.org/W2088180120","https://openalex.org/W2090280039","https://openalex.org/W2095723260","https://openalex.org/W2097446809","https://openalex.org/W2098010659","https://openalex.org/W2103037419","https://openalex.org/W2109038233","https://openalex.org/W2121438595","https://openalex.org/W2129536471","https://openalex.org/W2131869707","https://openalex.org/W2133425351","https://openalex.org/W2162255944","https://openalex.org/W2192820489","https://openalex.org/W2568345024","https://openalex.org/W2752829059","https://openalex.org/W2753422859","https://openalex.org/W2785551232","https://openalex.org/W2951839115","https://openalex.org/W2952772847"],"related_works":["https://openalex.org/W3215107043","https://openalex.org/W7222135401","https://openalex.org/W3125731338","https://openalex.org/W4254903595","https://openalex.org/W4390704263","https://openalex.org/W7160550636","https://openalex.org/W2783273533","https://openalex.org/W2032024429","https://openalex.org/W3084579536","https://openalex.org/W1976528444"],"abstract_inverted_index":{"ABSTRACT":[0],"The":[1],"widespread":[2,63],"use":[3],"of":[4,21,27,32,48,77,102,106,141],"antibiotics":[5],"across":[6,52],"clinical":[7],"and":[8,16,68,114],"agricultural":[9],"settings":[10],"results":[11,111],"in":[12,138],"strong":[13],"selection":[14],"pressures":[15],"contributes":[17],"to":[18,61,70,85,118,122,125,134,143],"the":[19,25,30,45,78,100,107,139],"fixation":[20],"antibiotic":[22,49],"resistance":[23,50,69],"genes,":[24],"presence":[26],"which":[28],"lowers":[29],"efficacy":[31],"proven":[33],"treatments":[34],"for":[35],"infection.":[36],"Furthermore,":[37],"plasmids":[38],"are":[39],"often":[40],"key":[41],"vectors":[42],"that":[43,75],"facilitate":[44],"rapid":[46],"dispersal":[47],"genes":[51],"bacterial":[53],"strains":[54,84],"via":[55],"horizontal":[56,135],"gene":[57,136],"transfer.":[58],"In":[59],"contrast":[60],"previous":[62],"correlations":[64],"between":[65],"plasmid":[66,126],"acquisition":[67,76,101,127],"antimicrobial":[71],"compounds,":[72],"we":[73,98],"demonstrate":[74],"P.":[79],"syringae":[80],"megaplasmid":[81],"pMPPla107":[82,103],"sensitizes":[83],"a":[86],"conserved,":[87],"bacteriostatic":[88],"small":[89],"molecule":[90],"produced":[91],"by":[92],"many":[93],"Pseudomonas":[94],"spp":[95],".":[96],"Moreover,":[97],"find":[99],"reduces":[104],"production":[105],"inhibitory":[108],"agent.":[109],"Our":[110],"provide":[112],"insights":[113],"suggest":[115],"new":[116],"directions":[117],"investigate":[119],"collateral":[120],"sensitivity":[121,142],"antimicrobials":[123],"due":[124],"as":[128,130],"well":[129],"highlight":[131],"costs":[132],"associated":[133],"transfer":[137],"form":[140],"antagonistic":[144],"microbial":[145],"interactions.":[146]},"counts_by_year":[{"year":2021,"cited_by_count":1}],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2019-02-21T00:00:00"}