{"id":"https://openalex.org/W2913580792","doi":"https://doi.org/10.3791/58710","title":"Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays","display_name":"Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays","publication_year":2019,"publication_date":"2019-01-07","ids":{"openalex":"https://openalex.org/W2913580792","doi":"https://doi.org/10.3791/58710","mag":"2913580792","pmid":"https://pubmed.ncbi.nlm.nih.gov/30663717"},"language":"en","primary_location":{"id":"doi:10.3791/58710","doi":"https://doi.org/10.3791/58710","is_oa":false,"landing_page_url":"https://doi.org/10.3791/58710","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5047781859","display_name":"Anagha J. Deshpande","orcid":"https://orcid.org/0000-0002-5653-2837","observed_orcids":["https://orcid.org/0000-0002-5653-2837"]},"institutions":[{"id":"https://openalex.org/I188891513","display_name":"Sanford Burnham Prebys Medical Discovery Institute","ror":"https://ror.org/03m1g2s55","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I188891513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anagha Deshpande","raw_affiliation_strings":["Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute","Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute; adeshpande@SBPdiscovery.org"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute","institution_ids":["https://openalex.org/I188891513"]},{"raw_affiliation_string":"Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute; adeshpande@SBPdiscovery.org","institution_ids":["https://openalex.org/I188891513"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049921263","display_name":"Bo Rui Chen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I188891513","display_name":"Sanford Burnham Prebys Medical Discovery Institute","ror":"https://ror.org/03m1g2s55","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I188891513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bo Rui Chen","raw_affiliation_strings":["Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute","institution_ids":["https://openalex.org/I188891513"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100615572","display_name":"Luyi Zhao","orcid":"https://orcid.org/0009-0001-6520-4050","observed_orcids":["https://orcid.org/0009-0001-6520-4050"]},"institutions":[{"id":"https://openalex.org/I188891513","display_name":"Sanford Burnham Prebys Medical Discovery Institute","ror":"https://ror.org/03m1g2s55","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I188891513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Luyi Zhao","raw_affiliation_strings":["Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute","institution_ids":["https://openalex.org/I188891513"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091765050","display_name":"Kelsey Saddoris","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I188891513","display_name":"Sanford Burnham Prebys Medical Discovery Institute","ror":"https://ror.org/03m1g2s55","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I188891513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kelsey Saddoris","raw_affiliation_strings":["Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute","institution_ids":["https://openalex.org/I188891513"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065172116","display_name":"Mayuri Kerr","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I188891513","display_name":"Sanford Burnham Prebys Medical Discovery Institute","ror":"https://ror.org/03m1g2s55","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I188891513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mayuri Kerr","raw_affiliation_strings":["Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute","institution_ids":["https://openalex.org/I188891513"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031550596","display_name":"Nan Zhu","orcid":"https://orcid.org/0000-0001-8568-1295","observed_orcids":["https://orcid.org/0000-0001-8568-1295","https://orcid.org/0009-0008-8059-4019","https://orcid.org/0009-0009-3509-766X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nan Zhu","raw_affiliation_strings":["Blood Research Institute, Blood Center of Wisconsin"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Blood Research Institute, Blood Center of Wisconsin","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102747646","display_name":"Prashant Mali","orcid":"https://orcid.org/0000-0002-3383-1287","observed_orcids":["https://orcid.org/0000-0002-3383-1287"]},"institutions":[{"id":"https://openalex.org/I36258959","display_name":"University of California San Diego","ror":"https://ror.org/0168r3w48","country_code":"US","type":"education","lineage":["https://openalex.org/I36258959"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Prashant Mali","raw_affiliation_strings":["Department of Bioengineering, University of California, San Diego"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Bioengineering, University of California, San Diego","institution_ids":["https://openalex.org/I36258959"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009399465","display_name":"Aniruddha J. Deshpande","orcid":"https://orcid.org/0000-0002-5240-9356","observed_orcids":["https://orcid.org/0000-0002-5240-9356"]},"institutions":[{"id":"https://openalex.org/I188891513","display_name":"Sanford Burnham Prebys Medical Discovery Institute","ror":"https://ror.org/03m1g2s55","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I188891513"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aniruddha J. Deshpande","raw_affiliation_strings":["Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute","Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute; adeshpande@SBPdiscovery.org"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute","institution_ids":["https://openalex.org/I188891513"]},{"raw_affiliation_string":"Tumor Initiation and Maintenance Program, Sanford Burnham Prebys Medical Discovery Institute; adeshpande@SBPdiscovery.org","institution_ids":["https://openalex.org/I188891513"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.1744,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.50182657,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":null,"issue":"143","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":0.8701000213623047,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":0.8701000213623047,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.11710000038146973,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12820","display_name":"Chromatin Remodeling and Cancer","score":0.002400000113993883,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/acute-myeloid-leukemia","display_name":"acute myeloid leukemia","score":0.9940000176429749},{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.8429999947547913},{"id":"https://openalex.org/keywords/genetic-perturbation","display_name":"gene perturbation","score":0.7480000257492065},{"id":"https://openalex.org/keywords/high-throughput-flow-cytometry","display_name":"high-throughput flow cytometry","score":0.6579999923706055},{"id":"https://openalex.org/keywords/gene-knockout","display_name":"gene knockout","score":0.47699999809265137},{"id":"https://openalex.org/keywords/leukemia-cell-lines","display_name":"leukemia cell lines","score":0.593999981880188},{"id":"https://openalex.org/keywords/genetic-dependencies","display_name":"gene dependency","score":0.515999972820282}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.9156049489974976},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.7542767524719238},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6630287170410156},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.6516575813293457},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6004135012626648},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5935020446777344},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.519321084022522},{"id":"https://openalex.org/C69991583","wikidata":"https://www.wikidata.org/wiki/Q1423575","display_name":"Candidate gene","level":3,"score":0.46883028745651245},{"id":"https://openalex.org/C207177822","wikidata":"https://www.wikidata.org/wiki/Q5050812","display_name":"Genetic screen","level":4,"score":0.4503192603588104},{"id":"https://openalex.org/C77957584","wikidata":"https://www.wikidata.org/wiki/Q1133550","display_name":"Gene knockout","level":3,"score":0.44614240527153015},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.4452546238899231},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.44495266675949097}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018345","descriptor_name":"Mice, Knockout","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.3791/58710","doi":"https://doi.org/10.3791/58710","is_oa":false,"landing_page_url":"https://doi.org/10.3791/58710","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"doi:10.3791/58710-v","doi":"https://doi.org/10.3791/58710-v","is_oa":false,"landing_page_url":"https://doi.org/10.3791/58710-v","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmid:30663717","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30663717","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.504800021648407,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G9000864383","display_name":"Cancer Center Support Grant (CCSG)","funder_award_id":"P30CA030199","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320306536","display_name":"V Foundation for Cancer Research","ror":"https://ror.org/00kbbk236"},{"id":"https://openalex.org/F4320314070","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":16,"referenced_works":["https://openalex.org/W1635615463","https://openalex.org/W1934704664","https://openalex.org/W1968677867","https://openalex.org/W1975887213","https://openalex.org/W2003171404","https://openalex.org/W2050480410","https://openalex.org/W2059171174","https://openalex.org/W2069918359","https://openalex.org/W2089589904","https://openalex.org/W2091208410","https://openalex.org/W2182456467","https://openalex.org/W2228518708","https://openalex.org/W2592787461","https://openalex.org/W2952346338","https://openalex.org/W4241093998","https://openalex.org/W4299422128"],"related_works":["https://openalex.org/W2747116494","https://openalex.org/W2515809353","https://openalex.org/W2943843423","https://openalex.org/W2977177111","https://openalex.org/W3200090243","https://openalex.org/W2745470532","https://openalex.org/W4417009189","https://openalex.org/W4200513116","https://openalex.org/W4379283231","https://openalex.org/W7131672785"],"abstract_inverted_index":{"Gene":[0],"perturbation":[1],"studies":[2,25,36],"have":[3,26,87],"been":[4],"extensively":[5],"used":[6,113],"to":[7,92,114,168],"investigate":[8,169],"the":[9,75,107,121,136,141,170,181],"role":[10,62,171,179],"of":[11,23,29,78,103,129,135,143,147,172,185],"individual":[12],"genes":[13,58,148,173],"in":[14,63,69,84,120,149,180],"AML":[15,64,70,189],"pathogenesis.":[16],"For":[17],"achieving":[18],"complete":[19],"gene":[20,31],"disruption,":[21],"many":[22],"these":[24,35],"made":[27],"use":[28],"complex":[30],"knockout":[32,38],"models.":[33],"While":[34],"with":[37,163],"mice":[39],"offer":[40],"an":[41,97,177],"elegant":[42],"and":[43,52,117,127,187],"time-tested":[44],"system":[45,102],"for":[46,55,140],"investigating":[47],"genotype-to-phenotype":[48],"relationships,":[49],"a":[50,61,144,155],"rapid":[51,116],"scalable":[53],"method":[54,109],"assessing":[56],"candidate":[57,80],"that":[59,110,174],"play":[60,176],"cell":[65,123,190],"proliferation":[66,182],"or":[67,183],"survival":[68,184],"models":[71],"will":[72],"help":[73],"accelerate":[74],"parallel":[76],"interrogation":[77,142],"multiple":[79],"genes.":[81],"Recent":[82],"advances":[83],"genome-editing":[85],"technologies":[86],"dramatically":[88],"enhanced":[89],"our":[90],"ability":[91],"perform":[93],"genetic":[94],"perturbations":[95],"at":[96],"unprecedented":[98],"scale.":[99],"One":[100],"such":[101],"genome":[104],"editing":[105],"is":[106],"CRISPR-Cas9-based":[108],"can":[111],"be":[112],"make":[115],"efficacious":[118],"alterations":[119],"target":[122],"genome.":[124],"The":[125],"ease":[126],"scalability":[128],"CRISPR/Cas9-mediated":[130],"gene-deletion":[131],"makes":[132],"it":[133],"one":[134],"most":[137],"attractive":[138],"techniques":[139],"large":[145],"number":[146],"phenotypic":[150],"assays.":[151],"Here,":[152],"we":[153],"present":[154],"simple":[156],"assay":[157],"using":[158],"CRISPR/Cas9":[159],"mediated":[160],"gene-disruption":[161],"combined":[162],"high-throughput":[164],"flow-cytometry-based":[165],"competition":[166],"assays":[167],"may":[175],"important":[178],"human":[186],"murine":[188],"lines.":[191]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2019-02-21T00:00:00"}
