{"id":"https://openalex.org/W2913299251","doi":"https://doi.org/10.1016/j.pep.2019.01.012","title":"Expression of recombinant tachyplesin I in Pichia pastoris","display_name":"Expression of recombinant tachyplesin I in Pichia pastoris","publication_year":2019,"publication_date":"2019-02-01","ids":{"openalex":"https://openalex.org/W2913299251","doi":"https://doi.org/10.1016/j.pep.2019.01.012","mag":"2913299251","pmid":"https://pubmed.ncbi.nlm.nih.gov/30711625"},"language":"en","primary_location":{"id":"doi:10.1016/j.pep.2019.01.012","doi":"https://doi.org/10.1016/j.pep.2019.01.012","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.pep.2019.01.012","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101743895","display_name":"Hanmei Li","orcid":"https://orcid.org/0000-0003-3648-6041","observed_orcids":["https://orcid.org/0000-0003-3648-6041"]},"institutions":[{"id":"https://openalex.org/I182722699","display_name":"Shenzhen Polytechnic University","ror":"https://ror.org/00d2w9g53","country_code":"CN","type":"education","lineage":["https://openalex.org/I182722699"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Hanmei Li","raw_affiliation_strings":["Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China","institution_ids":["https://openalex.org/I182722699"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100683784","display_name":"Zeeshan Ali","orcid":"https://orcid.org/0000-0001-8567-1177","observed_orcids":["https://orcid.org/0000-0001-8567-1177"]},"institutions":[{"id":"https://openalex.org/I182722699","display_name":"Shenzhen Polytechnic University","ror":"https://ror.org/00d2w9g53","country_code":"CN","type":"education","lineage":["https://openalex.org/I182722699"]},{"id":"https://openalex.org/I76569877","display_name":"Southeast University","ror":"https://ror.org/04ct4d772","country_code":"CN","type":"education","lineage":["https://openalex.org/I76569877"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zeeshan Ali","raw_affiliation_strings":["Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China; School of Medicine, Southeast University, Nanjing, 210009, China","School of Medicine, Southeast University, Nanjing, 210009, China","Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China; School of Medicine, Southeast University, Nanjing, 210009, China","institution_ids":["https://openalex.org/I182722699","https://openalex.org/I76569877"]},{"raw_affiliation_string":"School of Medicine, Southeast University, Nanjing, 210009, China","institution_ids":["https://openalex.org/I76569877"]},{"raw_affiliation_string":"Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China","institution_ids":["https://openalex.org/I182722699"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100393815","display_name":"Xiaolong Liu","orcid":"https://orcid.org/0000-0002-3096-4981","observed_orcids":["https://orcid.org/0000-0002-3096-4981","https://orcid.org/0009-0003-2827-2155","https://orcid.org/0009-0001-2180-3158","https://orcid.org/0009-0006-8941-1085"]},"institutions":[{"id":"https://openalex.org/I182722699","display_name":"Shenzhen Polytechnic University","ror":"https://ror.org/00d2w9g53","country_code":"CN","type":"education","lineage":["https://openalex.org/I182722699"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiaolong Liu","raw_affiliation_strings":["Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China","institution_ids":["https://openalex.org/I182722699"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100817777","display_name":"Li Jiang","orcid":"https://orcid.org/0000-0001-8504-7497","observed_orcids":["https://orcid.org/0000-0001-8504-7497"]},"institutions":[{"id":"https://openalex.org/I76569877","display_name":"Southeast University","ror":"https://ror.org/04ct4d772","country_code":"CN","type":"education","lineage":["https://openalex.org/I76569877"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Li Jiang","raw_affiliation_strings":["School of Medicine, Southeast University, Nanjing, 210009, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Medicine, Southeast University, Nanjing, 210009, China","institution_ids":["https://openalex.org/I76569877"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026527454","display_name":"Yongjun Tang","orcid":"https://orcid.org/0000-0002-7526-1112","observed_orcids":["https://orcid.org/0000-0002-7526-1112"]},"institutions":[{"id":"https://openalex.org/I182722699","display_name":"Shenzhen Polytechnic University","ror":"https://ror.org/00d2w9g53","country_code":"CN","type":"education","lineage":["https://openalex.org/I182722699"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Yongjun Tang","raw_affiliation_strings":["Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China. Electronic address: tangyongjun@szpt.edu.cn","Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China. Electronic address: tangyongjun@szpt.edu.cn","institution_ids":["https://openalex.org/I182722699"]},{"raw_affiliation_string":"Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China","institution_ids":["https://openalex.org/I182722699"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5102086608","display_name":"Jianguo Dai","orcid":"https://orcid.org/0000-0001-9904-7914","observed_orcids":["https://orcid.org/0000-0001-9904-7914"]},"institutions":[{"id":"https://openalex.org/I182722699","display_name":"Shenzhen Polytechnic University","ror":"https://ror.org/00d2w9g53","country_code":"CN","type":"education","lineage":["https://openalex.org/I182722699"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Jianguo Dai","raw_affiliation_strings":["Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China. Electronic address: jgdai@szpt.edu.cn","Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China. Electronic address: jgdai@szpt.edu.cn","institution_ids":["https://openalex.org/I182722699"]},{"raw_affiliation_string":"Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen, 518055, China","institution_ids":["https://openalex.org/I182722699"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5026527454","https://openalex.org/A5102086608"],"corresponding_institution_ids":["https://openalex.org/I182722699"],"apc_list":{"value":3300,"currency":"USD","value_usd":3300},"apc_paid":null,"fwci":0.8204,"has_fulltext":false,"cited_by_count":14,"citation_normalized_percentile":{"value":0.66517205,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"157","issue":null,"first_page":"50","last_page":"56"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11103","display_name":"Antimicrobial Peptides and Activities","score":0.8281000256538391,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11103","display_name":"Antimicrobial Peptides and Activities","score":0.8281000256538391,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.14069999754428864,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.006200000178068876,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tachyplesin-i","display_name":"tachyplesin I","score":0.9990000128746033},{"id":"https://openalex.org/keywords/antimicrobial-peptides","display_name":"antimicrobial peptides","score":0.8820000290870667},{"id":"https://openalex.org/keywords/komagataella-phaffii","display_name":"Pichia pastoris","score":0.9409999847412109},{"id":"https://openalex.org/keywords/recombinant-peptide-expression","display_name":"recombinant peptide expression","score":0.8820000290870667},{"id":"https://openalex.org/keywords/bactericidal-activity","display_name":"bactericidal activity","score":0.7739999890327454},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8600000143051147},{"id":"https://openalex.org/keywords/staphylococcus-aureus","display_name":"Staphylococcus aureus","score":0.9129999876022339},{"id":"https://openalex.org/keywords/pseudomonas-aeruginosa","display_name":"Pseudomonas aeruginosa","score":0.9430000185966492},{"id":"https://openalex.org/keywords/bacillus-subtilis","display_name":"Bacillus subtilis","score":0.953000009059906},{"id":"https://openalex.org/keywords/antibiotic-resistance","display_name":"antibiotic resistance","score":0.44200000166893005}],"concepts":[{"id":"https://openalex.org/C2781372317","wikidata":"https://www.wikidata.org/wiki/Q148697","display_name":"Pichia pastoris","level":4,"score":0.9771163463592529},{"id":"https://openalex.org/C33161422","wikidata":"https://www.wikidata.org/wiki/Q5421712","display_name":"Expression vector","level":4,"score":0.5875031352043152},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5734686851501465},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5661740899085999},{"id":"https://openalex.org/C2779703480","wikidata":"https://www.wikidata.org/wiki/Q125153","display_name":"Pichia","level":5,"score":0.5122422575950623},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4983797073364258},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4658876955509186},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.44971996545791626},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.4417193531990051},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3999595046043396},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33574700355529785}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004268","descriptor_name":"DNA-Binding Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004268","descriptor_name":"DNA-Binding Proteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006737","descriptor_name":"Horseshoe Crabs","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010456","descriptor_name":"Peptides, Cyclic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010456","descriptor_name":"Peptides, Cyclic","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011550","descriptor_name":"Pseudomonas aeruginosa","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D013211","descriptor_name":"Staphylococcus aureus","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D023181","descriptor_name":"Antimicrobial Cationic Peptides","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D023181","descriptor_name":"Antimicrobial Cationic Peptides","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D060829","descriptor_name":"Arthropod Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D060829","descriptor_name":"Arthropod Proteins","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.pep.2019.01.012","doi":"https://doi.org/10.1016/j.pep.2019.01.012","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.pep.2019.01.012","pdf_url":null,"source":{"id":"https://openalex.org/S9184782","display_name":"Protein Expression and Purification","issn_l":"1046-5928","issn":["1046-5928","1096-0279"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein Expression and Purification","raw_type":"journal-article"},{"id":"pmid:30711625","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30711625","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Protein expression and purification","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W79371963","https://openalex.org/W1483201238","https://openalex.org/W1849320643","https://openalex.org/W1953843785","https://openalex.org/W1970621669","https://openalex.org/W1979338300","https://openalex.org/W1991656269","https://openalex.org/W1991689677","https://openalex.org/W1994921326","https://openalex.org/W2019083571","https://openalex.org/W2023272764","https://openalex.org/W2026491949","https://openalex.org/W2027020919","https://openalex.org/W2031630638","https://openalex.org/W2038984318","https://openalex.org/W2041998654","https://openalex.org/W2045221958","https://openalex.org/W2046437093","https://openalex.org/W2049346716","https://openalex.org/W2056304926","https://openalex.org/W2058565700","https://openalex.org/W2058830577","https://openalex.org/W2066041816","https://openalex.org/W2080250706","https://openalex.org/W2081635117","https://openalex.org/W2124238798","https://openalex.org/W2124941037","https://openalex.org/W2131192673","https://openalex.org/W2136723682","https://openalex.org/W2767263894","https://openalex.org/W2772002858","https://openalex.org/W4214630015","https://openalex.org/W4255507877"],"related_works":["https://openalex.org/W2074209782","https://openalex.org/W2350845865","https://openalex.org/W2369293780","https://openalex.org/W1999739791","https://openalex.org/W965327363","https://openalex.org/W2376345639","https://openalex.org/W3024353989","https://openalex.org/W2336719217","https://openalex.org/W2388382871","https://openalex.org/W2473124202"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2019-02-21T00:00:00"}