{"id":"https://openalex.org/W2912936409","doi":"https://doi.org/10.1007/s10529-019-02645-x","title":"Pinpointing the l-phenylalanine binding sites of TyrR using biosensors and computer-aided simulation","display_name":"Pinpointing the l-phenylalanine binding sites of TyrR using biosensors and computer-aided simulation","publication_year":2019,"publication_date":"2019-01-24","ids":{"openalex":"https://openalex.org/W2912936409","doi":"https://doi.org/10.1007/s10529-019-02645-x","mag":"2912936409","pmid":"https://pubmed.ncbi.nlm.nih.gov/30680497"},"language":"en","primary_location":{"id":"doi:10.1007/s10529-019-02645-x","doi":"https://doi.org/10.1007/s10529-019-02645-x","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s10529-019-02645-x","pdf_url":null,"source":{"id":"https://openalex.org/S113993733","display_name":"Biotechnology Letters","issn_l":"0141-5492","issn":["0141-5492","1573-6776"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Letters","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5039766784","display_name":"Danyang Bai","orcid":"https://orcid.org/0009-0003-6585-3688","observed_orcids":["https://orcid.org/0009-0003-6585-3688"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210139473","display_name":"Tianjin Institute of Industrial Biotechnology","ror":"https://ror.org/042pyga86","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]},{"id":"https://openalex.org/I88372448","display_name":"Dalian Polytechnic University","ror":"https://ror.org/00c7x4a95","country_code":"CN","type":"education","lineage":["https://openalex.org/I88372448"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Danyang Bai","raw_affiliation_strings":["School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China","Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China","institution_ids":["https://openalex.org/I88372448"]},{"raw_affiliation_string":"Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050158840","display_name":"Dongqin Ding","orcid":"https://orcid.org/0000-0002-5775-1943","observed_orcids":["https://orcid.org/0000-0002-5775-1943"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210139473","display_name":"Tianjin Institute of Industrial Biotechnology","ror":"https://ror.org/042pyga86","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]},{"id":"https://openalex.org/I4210165038","display_name":"University of Chinese Academy of Sciences","ror":"https://ror.org/05qbk4x57","country_code":"CN","type":"education","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210165038"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Dongqin Ding","raw_affiliation_strings":["Key Laboratories of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China","Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China","University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratories of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China","institution_ids":["https://openalex.org/I19820366"]},{"raw_affiliation_string":"Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]},{"raw_affiliation_string":"University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China","institution_ids":["https://openalex.org/I4210165038"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100454984","display_name":"Jinlong Li","orcid":"https://orcid.org/0000-0002-8348-2476","observed_orcids":["https://orcid.org/0000-0002-8348-2476"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210139473","display_name":"Tianjin Institute of Industrial Biotechnology","ror":"https://ror.org/042pyga86","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jinlong Li","raw_affiliation_strings":["Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100299980","display_name":"Lina Cong","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I88372448","display_name":"Dalian Polytechnic University","ror":"https://ror.org/00c7x4a95","country_code":"CN","type":"education","lineage":["https://openalex.org/I88372448"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Lina Cong","raw_affiliation_strings":["School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China. linacong@163.com","School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China. linacong@163.com","institution_ids":["https://openalex.org/I88372448"]},{"raw_affiliation_string":"School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, People's Republic of China","institution_ids":["https://openalex.org/I88372448"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100379732","display_name":"Dawei Zhang","orcid":"https://orcid.org/0000-0001-5555-6151","observed_orcids":["https://orcid.org/0000-0001-5555-6151","https://orcid.org/0000-0002-5857-394X"]},"institutions":[{"id":"https://openalex.org/I19820366","display_name":"Chinese Academy of Sciences","ror":"https://ror.org/034t30j35","country_code":"CN","type":"government","lineage":["https://openalex.org/I19820366"]},{"id":"https://openalex.org/I4210139473","display_name":"Tianjin Institute of Industrial Biotechnology","ror":"https://ror.org/042pyga86","country_code":"CN","type":"nonprofit","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]},{"id":"https://openalex.org/I4210165038","display_name":"University of Chinese Academy of Sciences","ror":"https://ror.org/05qbk4x57","country_code":"CN","type":"education","lineage":["https://openalex.org/I19820366","https://openalex.org/I4210165038"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Dawei Zhang","raw_affiliation_strings":["Key Laboratories of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China. zhang_dw@tib.cas.cn","Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China. zhang_dw@tib.cas.cn","University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China. zhang_dw@tib.cas.cn","Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Key Laboratories of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China. zhang_dw@tib.cas.cn","institution_ids":["https://openalex.org/I19820366"]},{"raw_affiliation_string":"Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China. zhang_dw@tib.cas.cn","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]},{"raw_affiliation_string":"University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China. zhang_dw@tib.cas.cn","institution_ids":["https://openalex.org/I4210165038"]},{"raw_affiliation_string":"Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China","institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210139473"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5100299980","https://openalex.org/A5100379732"],"corresponding_institution_ids":["https://openalex.org/I19820366","https://openalex.org/I4210139473","https://openalex.org/I4210165038","https://openalex.org/I88372448"],"apc_list":{"value":3140,"currency":"USD","value_usd":3140},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.00759683,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"41","issue":"3","first_page":"401","last_page":"408"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.8036999702453613,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.8036999702453613,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.1525000035762787,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.015300000086426735,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tyrr","display_name":"TyrR","score":0.8759999871253967},{"id":"https://openalex.org/keywords/ligand-binding","display_name":"ligand binding","score":0.5130000114440918},{"id":"https://openalex.org/keywords/transcription-factors","display_name":"transcription factors","score":0.675000011920929},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8159999847412109},{"id":"https://openalex.org/keywords/aromatic-amino-acid-biosynthesis","display_name":"aromatic amino acid biosynthesis","score":0.8299999833106995},{"id":"https://openalex.org/keywords/biosensors","display_name":"biosensors","score":0.5389999747276306},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"site-directed mutagenesis","score":0.843999981880188},{"id":"https://openalex.org/keywords/molecular-simulation","display_name":"molecular simulation","score":0.5419999957084656},{"id":"https://openalex.org/keywords/transcriptional-activation","display_name":"transcriptional activation","score":0.6600000262260437}],"concepts":[{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.5838819742202759},{"id":"https://openalex.org/C2777431362","wikidata":"https://www.wikidata.org/wiki/Q170545","display_name":"Phenylalanine","level":3,"score":0.5789141058921814},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5575559735298157},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5449811220169067},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5034977793693542},{"id":"https://openalex.org/C53105671","wikidata":"https://www.wikidata.org/wiki/Q14777816","display_name":"Rational design","level":2,"score":0.4617841839790344},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4531335234642029},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.4435359239578247},{"id":"https://openalex.org/C2777992278","wikidata":"https://www.wikidata.org/wiki/Q705605","display_name":"Aromatic amino acids","level":3,"score":0.43189865350723267},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.36448150873184204},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3442223370075226},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.33819687366485596},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2493819296360016},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.08529520034790039}],"mesh":[{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004252","descriptor_name":"DNA Mutational Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D010649","descriptor_name":"Phenylalanine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012097","descriptor_name":"Repressor Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012097","descriptor_name":"Repressor Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012097","descriptor_name":"Repressor Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015374","descriptor_name":"Biosensing Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D029968","descriptor_name":"Escherichia coli Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D056004","descriptor_name":"Molecular Dynamics Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/s10529-019-02645-x","doi":"https://doi.org/10.1007/s10529-019-02645-x","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s10529-019-02645-x","pdf_url":null,"source":{"id":"https://openalex.org/S113993733","display_name":"Biotechnology Letters","issn_l":"0141-5492","issn":["0141-5492","1573-6776"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Letters","raw_type":"journal-article"},{"id":"pmid:30680497","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30680497","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology letters","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1359892573","display_name":null,"funder_award_id":"16JCYBJC23500","funder_id":"https://openalex.org/F3057939197","funder_display_name":"Comanche Nation"},{"id":"https://openalex.org/G1432327317","display_name":null,"funder_award_id":"2018YFD0901001","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"},{"id":"https://openalex.org/G2514793472","display_name":null,"funder_award_id":"17JCJQJC45300","funder_id":"https://openalex.org/F4320336823","funder_display_name":"Science Fund for Distinguished Young Scholars of Tianjin"}],"funders":[{"id":"https://openalex.org/F3057939197","display_name":"Comanche Nation","ror":"https://ror.org/039rx3k87"},{"id":"https://openalex.org/F4320335777","display_name":"National Key Research and Development Program of China","ror":null},{"id":"https://openalex.org/F4320336823","display_name":"Science Fund for Distinguished Young Scholars of Tianjin","ror":null}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W1494450975","https://openalex.org/W1546783991","https://openalex.org/W1882082207","https://openalex.org/W2032840501","https://openalex.org/W2064943639","https://openalex.org/W2129629886","https://openalex.org/W2135018673","https://openalex.org/W2140538197","https://openalex.org/W2141957503","https://openalex.org/W2287529952","https://openalex.org/W2323778292","https://openalex.org/W2558343263"],"related_works":["https://openalex.org/W2094598322","https://openalex.org/W2186262857","https://openalex.org/W2129629886","https://openalex.org/W2135018673","https://openalex.org/W1803778303","https://openalex.org/W2117891907","https://openalex.org/W1546687907","https://openalex.org/W2038935524","https://openalex.org/W1580698658","https://openalex.org/W2094502799"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2019-02-21T00:00:00"}