{"id":"https://openalex.org/W2912908528","doi":"https://doi.org/10.1371/journal.pone.0211382","title":"Quantitative analysis of F-actin alterations in adherent human mesenchymal stem cells: Influence of slow-freezing and vitrification-based cryopreservation","display_name":"Quantitative analysis of F-actin alterations in adherent human mesenchymal stem cells: Influence of slow-freezing and vitrification-based cryopreservation","publication_year":2019,"publication_date":"2019-01-25","ids":{"openalex":"https://openalex.org/W2912908528","doi":"https://doi.org/10.1371/journal.pone.0211382","mag":"2912908528","pmid":"https://pubmed.ncbi.nlm.nih.gov/30682146","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6347223"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0211382","doi":"https://doi.org/10.1371/journal.pone.0211382","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0211382","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0211382&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","datacite","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0211382&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5022390237","display_name":"Yannik M\u00fcllers","orcid":"https://orcid.org/0000-0002-0475-3734","observed_orcids":["https://orcid.org/0000-0002-0475-3734"]},"institutions":[{"id":"https://openalex.org/I4210152467","display_name":"Fraunhofer Institute for Biomedical Engineering","ror":"https://ror.org/05tpsgh61","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4210152467","https://openalex.org/I4923324"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Yannik M\u00fcllers","raw_affiliation_strings":["Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany","institution_ids":["https://openalex.org/I4210152467"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008783880","display_name":"Ina Meiser","orcid":"https://orcid.org/0000-0003-1992-4580","observed_orcids":["https://orcid.org/0000-0003-1992-4580"]},"institutions":[{"id":"https://openalex.org/I4210152467","display_name":"Fraunhofer Institute for Biomedical Engineering","ror":"https://ror.org/05tpsgh61","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4210152467","https://openalex.org/I4923324"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ina Meiser","raw_affiliation_strings":["Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany","institution_ids":["https://openalex.org/I4210152467"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078121011","display_name":"Frank Stracke","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210152467","display_name":"Fraunhofer Institute for Biomedical Engineering","ror":"https://ror.org/05tpsgh61","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4210152467","https://openalex.org/I4923324"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Frank Stracke","raw_affiliation_strings":["Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany","institution_ids":["https://openalex.org/I4210152467"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020865150","display_name":"Iris Riemann","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210152467","display_name":"Fraunhofer Institute for Biomedical Engineering","ror":"https://ror.org/05tpsgh61","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4210152467","https://openalex.org/I4923324"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Iris Riemann","raw_affiliation_strings":["Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany","institution_ids":["https://openalex.org/I4210152467"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049333683","display_name":"Franziska Lautenschl\u00e4ger","orcid":"https://orcid.org/0000-0001-8263-3386","observed_orcids":["https://orcid.org/0000-0001-8263-3386"]},"institutions":[{"id":"https://openalex.org/I4210091344","display_name":"Leibniz-Institute for New Materials","ror":"https://ror.org/00g656d67","country_code":"DE","type":"facility","lineage":["https://openalex.org/I315704651","https://openalex.org/I4210091344","https://openalex.org/I91712215"]},{"id":"https://openalex.org/I91712215","display_name":"Saarland University","ror":"https://ror.org/01jdpyv68","country_code":"DE","type":"education","lineage":["https://openalex.org/I91712215"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Franziska Lautenschl\u00e4ger","raw_affiliation_strings":["Chair for Experimental Physics, Saarland University, Saarbr\u00fccken, Germany","Division of Cytoskeletal Fibers, Leibniz Institute for New Materials, Campus D2 2, Saarbr\u00fccken, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Chair for Experimental Physics, Saarland University, Saarbr\u00fccken, Germany","institution_ids":["https://openalex.org/I91712215"]},{"raw_affiliation_string":"Division of Cytoskeletal Fibers, Leibniz Institute for New Materials, Campus D2 2, Saarbr\u00fccken, Germany","institution_ids":["https://openalex.org/I4210091344"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012279683","display_name":"Julia Christiane Neubauer","orcid":"https://orcid.org/0000-0001-6876-1453","observed_orcids":["https://orcid.org/0000-0001-6876-1453"]},"institutions":[{"id":"https://openalex.org/I4210152467","display_name":"Fraunhofer Institute for Biomedical Engineering","ror":"https://ror.org/05tpsgh61","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4210152467","https://openalex.org/I4923324"]},{"id":"https://openalex.org/I4923324","display_name":"Fraunhofer-Gesellschaft","ror":"https://ror.org/05hkkdn48","country_code":"DE","type":"government","lineage":["https://openalex.org/I4923324"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Julia C. Neubauer","raw_affiliation_strings":["Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany","Fraunhofer Project Centre for Stem Cell Process Engineering, Neunerplatz 2, W\u00fcrzburg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany","institution_ids":["https://openalex.org/I4210152467"]},{"raw_affiliation_string":"Fraunhofer Project Centre for Stem Cell Process Engineering, Neunerplatz 2, W\u00fcrzburg, Germany","institution_ids":["https://openalex.org/I4923324"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5027494826","display_name":"Heiko Zimmermann","orcid":"https://orcid.org/0000-0001-8595-1304","observed_orcids":["https://orcid.org/0000-0001-8595-1304"]},"institutions":[{"id":"https://openalex.org/I2802166871","display_name":"Universidad Cat\u00f3lica del Norte","ror":"https://ror.org/02akpm128","country_code":"CL","type":"education","lineage":["https://openalex.org/I2802166871"]},{"id":"https://openalex.org/I4210152467","display_name":"Fraunhofer Institute for Biomedical Engineering","ror":"https://ror.org/05tpsgh61","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4210152467","https://openalex.org/I4923324"]},{"id":"https://openalex.org/I91712215","display_name":"Saarland University","ror":"https://ror.org/01jdpyv68","country_code":"DE","type":"education","lineage":["https://openalex.org/I91712215"]}],"countries":["CL","DE"],"is_corresponding":true,"raw_author_name":"Heiko Zimmermann","raw_affiliation_strings":["Chair for Molecular and Cellular Biotechnology, Saarland University, Saarbruecken, Germany","Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany","Faculty of Marine Science, Universidad Cat\u00f3lica del Norte, Coquimbo, Chile"],"raw_orcid":"https://orcid.org/0000-0001-8595-1304","affiliations":[{"raw_affiliation_string":"Chair for Molecular and Cellular Biotechnology, Saarland University, Saarbruecken, Germany","institution_ids":["https://openalex.org/I91712215"]},{"raw_affiliation_string":"Department of Cryo- and Stem Cell Technology, Fraunhofer Institute for Biomedical Engineering (IBMT), Joseph-von-Fraunhofer-Weg 1, Sulzbach, Germany","institution_ids":["https://openalex.org/I4210152467"]},{"raw_affiliation_string":"Faculty of Marine Science, Universidad Cat\u00f3lica del Norte, Coquimbo, Chile","institution_ids":["https://openalex.org/I2802166871"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5027494826"],"corresponding_institution_ids":["https://openalex.org/I2802166871","https://openalex.org/I4210152467","https://openalex.org/I91712215"],"apc_list":{"value":1595,"currency":"USD","value_usd":1595},"apc_paid":{"value":1595,"currency":"USD","value_usd":1595},"fwci":0.5454,"has_fulltext":true,"cited_by_count":19,"citation_normalized_percentile":{"value":0.54138492,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":95,"max":97},"biblio":{"volume":"14","issue":"1","first_page":"e0211382","last_page":"e0211382"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.5845000147819519,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.5845000147819519,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.16089999675750732,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.1265999972820282,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/actin-filaments","display_name":"F-actin","score":0.8420000076293945},{"id":"https://openalex.org/keywords/actin-cytoskeleton","display_name":"actin cytoskeleton","score":0.9940000176429749},{"id":"https://openalex.org/keywords/mesenchymal-stem-cells","display_name":"mesenchymal stem cells","score":0.9679999947547913},{"id":"https://openalex.org/keywords/cryopreservation","display_name":"cryopreservation","score":0.9599999785423279},{"id":"https://openalex.org/keywords/slow-freezing","display_name":"slow freezing","score":0.722000002861023},{"id":"https://openalex.org/keywords/vitrification","display_name":"vitrification","score":0.9959999918937683},{"id":"https://openalex.org/keywords/cryoinjury","display_name":"cryoinjury","score":0.6510000228881836},{"id":"https://openalex.org/keywords/filament-length","display_name":"filament length","score":0.703000009059906},{"id":"https://openalex.org/keywords/post-thaw-recovery","display_name":"post-thaw recovery","score":0.7820000052452087},{"id":"https://openalex.org/keywords/cryo-imaging","display_name":"cryo-imaging","score":0.49799999594688416},{"id":"https://openalex.org/keywords/two-photon-microscopy","display_name":"two-photon microscopy","score":0.7070000171661377},{"id":"https://openalex.org/keywords/freezing-rate","display_name":"freezing rate","score":0.6309999823570251}],"concepts":[{"id":"https://openalex.org/C179933525","wikidata":"https://www.wikidata.org/wiki/Q1144664","display_name":"Cryopreservation","level":3,"score":0.8432196378707886},{"id":"https://openalex.org/C142669718","wikidata":"https://www.wikidata.org/wiki/Q154626","display_name":"Cytoskeleton","level":3,"score":0.7856725454330444},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6801788210868835},{"id":"https://openalex.org/C125705527","wikidata":"https://www.wikidata.org/wiki/Q185269","display_name":"Actin","level":2,"score":0.634059727191925},{"id":"https://openalex.org/C14228908","wikidata":"https://www.wikidata.org/wiki/Q2920483","display_name":"Protein filament","level":2,"score":0.6030139923095703},{"id":"https://openalex.org/C2993400109","wikidata":"https://www.wikidata.org/wiki/Q329638","display_name":"Actin cytoskeleton","level":4,"score":0.5454264283180237},{"id":"https://openalex.org/C198826908","wikidata":"https://www.wikidata.org/wiki/Q1922379","display_name":"Mesenchymal stem cell","level":2,"score":0.4807862639427185},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.43904802203178406},{"id":"https://openalex.org/C2778022349","wikidata":"https://www.wikidata.org/wiki/Q1072553","display_name":"Vitrification","level":2,"score":0.4387356638908386},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.3247929513454437},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.3013303279876709},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.12216949462890625},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.11960121989250183},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.1015397310256958},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.05262589454650879}],"mesh":[{"descriptor_ui":"D000199","descriptor_name":"Actins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D000199","descriptor_name":"Actins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D005615","descriptor_name":"Freezing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008841","descriptor_name":"Actin Cytoskeleton","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015925","descriptor_name":"Cryopreservation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D036641","descriptor_name":"Microscopy, Fluorescence, Multiphoton","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059630","descriptor_name":"Mesenchymal Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D059630","descriptor_name":"Mesenchymal Stem Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":10,"locations":[{"id":"doi:10.1371/journal.pone.0211382","doi":"https://doi.org/10.1371/journal.pone.0211382","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0211382","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0211382&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:5339529","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC6347223","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmid:30682146","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30682146","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6347223","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC6347223/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:0b94bccfc69d433c913074b228f56936","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/0b94bccfc69d433c913074b228f56936","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 14, Iss 1, p e0211382 (2019)","raw_type":"article"},{"id":"pmh:oai:figshare.com:article/7634303","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/dataset/Quantitative_analysis_of_F-actin_alterations_in_adherent_human_mesenchymal_stem_cells_Influence_of_slow-freezing_and_vitrification-based_cryopreservation/7634303","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"},{"id":"pmh:oai:fraunhofer.de:N-549467","doi":null,"is_oa":true,"landing_page_url":"http://publica.fraunhofer.de/documents/N-549467.html","pdf_url":null,"source":{"id":"https://openalex.org/S4306400801","display_name":"Publikationsdatenbank der Fraunhofer-Gesellschaft (Fraunhofer-Gesellschaft)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4923324","host_organization_name":"Fraunhofer-Gesellschaft","host_organization_lineage":["https://openalex.org/I4923324"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Fraunhofer IBMT","raw_type":"Journal Article"},{"id":"pmh:oai:oa.tib.eu:123456789/4834","doi":null,"is_oa":true,"landing_page_url":"https://oa.tib.eu/renate/handle/123456789/4834","pdf_url":null,"source":{"id":"https://openalex.org/S7407052981","display_name":"RENATE","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2802635041","host_organization_name":"Technische Informationsbibliothek (TIB)","host_organization_lineage":["https://openalex.org/I2802635041"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS One","raw_type":"doc-type:Article"},{"id":"pmh:oai:publica.fraunhofer.de:publica/258265","doi":null,"is_oa":false,"landing_page_url":"https://publica.fraunhofer.de/handle/publica/258265","pdf_url":null,"source":{"id":"https://openalex.org/S4306400318","display_name":"Fraunhofer-Publica (Fraunhofer-Gesellschaft)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4923324","host_organization_name":"Fraunhofer-Gesellschaft","host_organization_lineage":["https://openalex.org/I4923324"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"journal article"},{"id":"doi:10.34657/105","doi":"https://doi.org/10.34657/105","is_oa":true,"landing_page_url":"https://doi.org/10.34657/105","pdf_url":null,"source":{"id":"https://openalex.org/S7407052981","display_name":"RENATE","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2802635041","host_organization_name":"Technische Informationsbibliothek (TIB)","host_organization_lineage":["https://openalex.org/I2802635041"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0211382","doi":"https://doi.org/10.1371/journal.pone.0211382","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0211382","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0211382&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.5683000087738037}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2912908528.pdf","grobid_xml":"https://content.openalex.org/works/W2912908528.grobid-xml"},"referenced_works_count":63,"referenced_works":["https://openalex.org/W1518531052","https://openalex.org/W1549796567","https://openalex.org/W1969173609","https://openalex.org/W1971156171","https://openalex.org/W1973271878","https://openalex.org/W1984428412","https://openalex.org/W1984769051","https://openalex.org/W1986411839","https://openalex.org/W1997913897","https://openalex.org/W2000113497","https://openalex.org/W2001299523","https://openalex.org/W2005431883","https://openalex.org/W2010244610","https://openalex.org/W2013902799","https://openalex.org/W2019922483","https://openalex.org/W2031046488","https://openalex.org/W2033641981","https://openalex.org/W2037372181","https://openalex.org/W2037616201","https://openalex.org/W2040169304","https://openalex.org/W2042113375","https://openalex.org/W2044895919","https://openalex.org/W2047351398","https://openalex.org/W2049509539","https://openalex.org/W2050860694","https://openalex.org/W2055922450","https://openalex.org/W2058267099","https://openalex.org/W2059442046","https://openalex.org/W2063251232","https://openalex.org/W2063790968","https://openalex.org/W2067335193","https://openalex.org/W2069030820","https://openalex.org/W2069574579","https://openalex.org/W2071282863","https://openalex.org/W2073057280","https://openalex.org/W2086474578","https://openalex.org/W2090898691","https://openalex.org/W2092720718","https://openalex.org/W2093081064","https://openalex.org/W2101245520","https://openalex.org/W2101361529","https://openalex.org/W2103128269","https://openalex.org/W2107059134","https://openalex.org/W2108970999","https://openalex.org/W2120629404","https://openalex.org/W2121438091","https://openalex.org/W2128649233","https://openalex.org/W2131074202","https://openalex.org/W2134208433","https://openalex.org/W2136761389","https://openalex.org/W2152460518","https://openalex.org/W2158870181","https://openalex.org/W2163893998","https://openalex.org/W2165673443","https://openalex.org/W2173701088","https://openalex.org/W2398957227","https://openalex.org/W2403471225","https://openalex.org/W2432891822","https://openalex.org/W2472969048","https://openalex.org/W2598022505","https://openalex.org/W3020890390","https://openalex.org/W4240278375","https://openalex.org/W4241047453"],"related_works":["https://openalex.org/W2122515221","https://openalex.org/W7097636858","https://openalex.org/W2073057280","https://openalex.org/W2063790968","https://openalex.org/W4402345430","https://openalex.org/W2004437766","https://openalex.org/W2101473213","https://openalex.org/W4378907424","https://openalex.org/W2037372181","https://openalex.org/W2123480459"],"abstract_inverted_index":{"Cryopreservation":[0],"is":[1,30,36,133,244,256],"an":[2,240],"essential":[3],"tool":[4],"to":[5,54,60],"meet":[6],"the":[7,24,27,41,79,87,113,120,140,175,184,193,217,235,266],"increasing":[8],"demand":[9],"for":[10,72],"stem":[11,66],"cells":[12,107,156,220,238,264],"in":[13,62,105,152,183,226,246,249],"medical":[14],"applications.":[15,74],"To":[16],"ensure":[17],"maintenance":[18],"of":[19,26,43,91,102,115,122,139,159,163,174,189,211,219,233,237,262],"cell":[20],"function":[21],"upon":[22],"thawing,":[23,199],"preservation":[25],"actin":[28,80,141,150,160,222,242],"cytoskeleton":[29,81,142,243],"crucial,":[31],"but":[32],"so":[33],"far":[34],"there":[35,179],"little":[37],"quantitative":[38],"data":[39],"on":[40,45,82],"influence":[42],"cryopreservation":[44,57,111,195],"cytoskeletal":[46,200],"structures.":[47],"For":[48],"this":[49],"reason,":[50],"our":[51,116],"study":[52],"aims":[53],"quantitatively":[55],"describe":[56],"induced":[58],"alterations":[59,101,151,170],"F-actin":[61,94,164,190],"adherent":[63],"human":[64],"mesenchymal":[65],"cells,":[67],"as":[68],"a":[69,209,259],"basic":[70],"model":[71],"biomedical":[73],"Here":[75],"we":[76],"have":[77],"characterised":[78],"single-cell":[83],"level":[84],"by":[85,135],"calculating":[86],"circular":[88],"standard":[89],"deviation":[90],"filament":[92,98,167],"orientation,":[93],"content,":[95],"and":[96,109,124,129,154,166,186],"average":[97],"length.":[99],"Cryo-induced":[100],"these":[103],"parameters":[104],"identical":[106],"pre":[108],"post":[110],"provide":[112],"basis":[114],"investigation.":[117],"Differences":[118],"between":[119],"impact":[121],"slow-freezing":[123],"vitrification":[125],"are":[126,180],"qualitatively":[127],"analyzed":[128],"highlighted.":[130],"Our":[131],"analysis":[132],"supported":[134],"live":[136],"cryo":[137],"imaging":[138],"via":[143],"two":[144],"photon":[145],"microscopy.":[146],"We":[147],"found":[148],"similar":[149],"slow-frozen":[153],"vitrified":[155,247],"including":[157],"buckling":[158],"filaments,":[161],"reduction":[162],"content":[165],"shortening.":[168],"These":[169],"indicate":[171],"limited":[172],"functionality":[173],"respective":[176],"cells.":[177,229,252],"However,":[178],"substantial":[181],"differences":[182],"frequency":[185],"time":[187],"dependence":[188],"disruptions":[191,223],"among":[192],"applied":[194],"strategies;":[196],"immediately":[197],"after":[198,205],"structures":[201],"show":[202],"least":[203],"disruption":[204],"slow":[206,227,250],"freezing":[207],"at":[208,254],"rate":[210],"1\u00b0C/min.":[212],"As":[213],"post-thaw":[214],"recovery":[215,234],"progresses,":[216],"ratio":[218,261],"with":[221,239,258],"increases,":[224],"particularly":[225],"frozen":[228,251],"After":[230],"120":[231],"min":[232],"proportion":[236],"intact":[241],"higher":[245],"than":[248],"Freezing":[253],"10\u00b0C/min":[255],"associated":[257],"high":[260],"impaired":[263],"throughout":[265],"post-thawing":[267],"culture.":[268]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2019-02-21T00:00:00"}
