{"id":"https://openalex.org/W29053392","doi":null,"title":"Isolation of C-reactive protein by affinity chromatography.","display_name":"Isolation of C-reactive protein by affinity chromatography.","publication_year":1977,"publication_date":"1977-10-01","ids":{"openalex":"https://openalex.org/W29053392","mag":"29053392","pmid":"https://pubmed.ncbi.nlm.nih.gov/23915","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1541158"},"language":"en","primary_location":{"id":"pmh:oai:pubmedcentral.nih.gov:1541158","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1541158","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clin Exp Immunol","raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1541158","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079554246","display_name":"Mark B. Pepys","orcid":"https://orcid.org/0000-0003-2614-3248","observed_orcids":["https://orcid.org/0000-0003-2614-3248"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M B Pepys","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"A C Dash","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A C Dash","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":null,"display_name":"M J Ashley","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M J Ashley","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.2714,"has_fulltext":false,"cited_by_count":77,"citation_normalized_percentile":{"value":0.86515539,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"30","issue":"1","first_page":"32","last_page":"7"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T14245","display_name":"Biomarkers in Disease Mechanisms","score":0.5083000063896179,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T14245","display_name":"Biomarkers in Disease Mechanisms","score":0.5083000063896179,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.4814000129699707,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/c-reactive-protein","display_name":"C-reactive protein","score":0.9990000128746033},{"id":"https://openalex.org/keywords/affinity-chromatography","display_name":"affinity chromatography","score":0.8650000095367432},{"id":"https://openalex.org/keywords/pneumococcal-c-polysaccharide","display_name":"pneumococcal C-polysaccharide","score":0.9110000133514404},{"id":"https://openalex.org/keywords/sepharose-4b","display_name":"Sepharose 4B","score":0.7260000109672546},{"id":"https://openalex.org/keywords/rocket-immunoelectrophoresis","display_name":"rocket immunoelectrophoresis","score":0.7620000243186951},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.5820000171661377}],"concepts":[{"id":"https://openalex.org/C2778476535","wikidata":"https://www.wikidata.org/wiki/Q390697","display_name":"Agarose","level":2,"score":0.8240867853164673},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7698898315429688},{"id":"https://openalex.org/C2777529624","wikidata":"https://www.wikidata.org/wiki/Q416475","display_name":"Glutaraldehyde","level":2,"score":0.7567513585090637},{"id":"https://openalex.org/C2779881004","wikidata":"https://www.wikidata.org/wiki/Q7451876","display_name":"Sepharose","level":3,"score":0.7556054592132568},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7386269569396973},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.7217631340026855},{"id":"https://openalex.org/C2777093645","wikidata":"https://www.wikidata.org/wiki/Q420258","display_name":"Cyanogen bromide","level":4,"score":0.6755568385124207},{"id":"https://openalex.org/C2778862431","wikidata":"https://www.wikidata.org/wiki/Q416002","display_name":"Polyacrylamide","level":2,"score":0.6186326146125793},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.577409029006958},{"id":"https://openalex.org/C2778660310","wikidata":"https://www.wikidata.org/wiki/Q177998","display_name":"Agar","level":3,"score":0.5484541058540344},{"id":"https://openalex.org/C73826308","wikidata":"https://www.wikidata.org/wiki/Q684907","display_name":"Elution","level":2,"score":0.5474876761436462},{"id":"https://openalex.org/C180577832","wikidata":"https://www.wikidata.org/wiki/Q127920","display_name":"Covalent bond","level":2,"score":0.46745190024375916},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3009527921676636},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.13932055234909058},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.1233745813369751},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.09457296133041382},{"id":"https://openalex.org/C188027245","wikidata":"https://www.wikidata.org/wiki/Q750446","display_name":"Polymer chemistry","level":1,"score":0.09309864044189453},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.08911445736885071},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000178","descriptor_name":"Acrylamides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000362","descriptor_name":"Agar","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002097","descriptor_name":"C-Reactive Protein","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002846","descriptor_name":"Chromatography, Affinity","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D005976","descriptor_name":"Glutaral","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007106","descriptor_name":"Immune Sera","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011135","descriptor_name":"Polysaccharides, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012685","descriptor_name":"Sepharose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013296","descriptor_name":"Streptococcus pneumoniae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"pmh:oai:pubmedcentral.nih.gov:1541158","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1541158","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clin Exp Immunol","raw_type":"Text"},{"id":"pmid:23915","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23915","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clinical and experimental immunology","raw_type":"Comparative Study"},{"id":"pmh:oai:eprints.ucl.ac.uk.OAI2:1552174","doi":null,"is_oa":false,"landing_page_url":"http://discovery.ucl.ac.uk/1552174/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400024","display_name":"UCL Discovery (University College London)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I45129253","host_organization_name":"University College London","host_organization_lineage":["https://openalex.org/I45129253"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Clin Exp Immunol , 30 (1) pp. 32-37. (1977)","raw_type":"Article"},{"id":"mag:29053392","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23915","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:1541158","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1541158","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Clin Exp Immunol","raw_type":"Text"},"sustainable_development_goals":[{"score":0.7246000170707703,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":8,"referenced_works":["https://openalex.org/W1562621556","https://openalex.org/W1604933883","https://openalex.org/W1977575870","https://openalex.org/W1990986783","https://openalex.org/W2001868765","https://openalex.org/W2064162862","https://openalex.org/W2342257955","https://openalex.org/W2403455550"],"related_works":["https://openalex.org/W2040849321","https://openalex.org/W1977575870","https://openalex.org/W2157851908","https://openalex.org/W2017577616","https://openalex.org/W2002611036","https://openalex.org/W2082081254","https://openalex.org/W2022108426","https://openalex.org/W2349483189","https://openalex.org/W30473567","https://openalex.org/W2033282842"],"abstract_inverted_index":{"Different":[0],"affinity":[1],"chromatography":[2],"methods":[3],"were":[4,122],"compared":[5],"for":[6,198,248],"the":[7,17,119,145,156,211,230,237,258],"isolation":[8],"of":[9,19,80,83,87,92,105,144,159,188,202,219,229,239,246,261],"C-reactive":[10],"protein":[11],"(CRP)":[12],"from":[13,124,221],"pathological":[14],"fluids.":[15],"In":[16],"presence":[18,238],"calcium":[20,240],"ions":[21],"CRP":[22,79,95,117,146,203,220],"became":[23],"bound":[24],"to":[25,31,47,64,127,137,166,175,213],"agar":[26,88],"(Ganrot":[27],"&":[28],"Kindmark,":[29],"1969),":[30],"cyanogen":[32],"bromide-activated":[33],"agarose":[34,234],"beads":[35,50,55,177,194,235],"(Sepharose":[36],"4B)":[37],"coupled":[38,59,136],"with":[39,60,74,182],"pneumococcal":[40,249],"C":[41,61,112,216,250],"substance":[42,62,113,126,251],"(Osmand":[43],"et":[44],"al.,":[45],"1975),":[46],"glutaraldehyde-activated":[48,138],"polyacrylamide":[49,66,176,193],"(Bio-Gel":[51,67],"P300)":[52,68],"or":[53,76],"agarose-acrylamide":[54],"(Ultrogel":[56],"AcA":[57],"34)":[58],"and":[63,118,170],"`sulphated'":[65,192],"beads.":[69,139],"After":[70],"thorough":[71],"washing,":[72],"elution":[73],"citrate":[75],"EDTA":[77],"yielded":[78],"varying":[81],"degrees":[82],"purity.":[84],"The":[85,140,186],"use":[86,187],"provided":[89,114],"high":[90],"yields":[91,121],"heavily":[93],"contaminated":[94,204],"which":[96,128,162,178],"was":[97,129,135,151,224,252],"substantially":[98],"purified":[99],"by":[100,206,227],"passage":[101],"through":[102],"a":[103,152,196],"column":[104],"insolubilized":[106],"anti-normal":[107],"human":[108],"serum":[109,154],"antibodies.":[110],"Insolubilized":[111],"much":[115],"purer":[116],"best":[120],"obtained":[123],"Sepharose\u2013CNBr\u2013C":[125],"attached":[130],"considerably":[131],"more":[132],"ligand":[133],"than":[134],"only":[141,205],"significant":[142],"contaminant":[143],"isolated":[147],"in":[148,236,257],"this":[149],"way":[150],"normal":[153],"protein,":[155],"P":[157,207,222,231],"component":[158,208,223,232],"amyloid":[160],"(Clt),":[161,209],"showed":[163],"calcium-dependent":[164],"binding":[165],"plain":[167],"unsubstituted":[168],"Sepharose":[169],"Ultrogel,":[171],"as":[172,174],"well":[173],"had":[179],"been":[180],"treated":[181],"concentrated":[183],"sulphuric":[184],"acid.":[185],"these":[189],"easily":[190],"prepared":[191],"provides":[195],"method":[197],"isolating":[199],"small":[200],"quantities":[201],"without":[210],"need":[212],"first":[214],"prepare":[215],"substance.":[217],"Separation":[218],"readily":[225],"achieved":[226],"absorption":[228],"onto":[233],"ions.":[241],"An":[242],"`electroimmunodiffusion',":[243],"`rocket'":[244],"type":[245],"assay":[247],"developed,":[253],"using":[254],"CRP-rich":[255],"fluid":[256],"gel":[259],"instead":[260],"antiserum.":[262]},"counts_by_year":[{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}