{"id":"https://openalex.org/W2903662858","doi":"https://doi.org/10.1111/ijlh.12954","title":"Evaluation of next\u2010generation sequencing\u2010based clonality analysis of T\u2010cell receptor gamma gene rearrangements based on a new interpretation algorithm","display_name":"Evaluation of next\u2010generation sequencing\u2010based clonality analysis of T\u2010cell receptor gamma gene rearrangements based on a new interpretation algorithm","publication_year":2018,"publication_date":"2018-12-08","ids":{"openalex":"https://openalex.org/W2903662858","doi":"https://doi.org/10.1111/ijlh.12954","mag":"2903662858","pmid":"https://pubmed.ncbi.nlm.nih.gov/30537135"},"language":"en","primary_location":{"id":"doi:10.1111/ijlh.12954","doi":"https://doi.org/10.1111/ijlh.12954","is_oa":false,"landing_page_url":"https://doi.org/10.1111/ijlh.12954","pdf_url":null,"source":{"id":"https://openalex.org/S66208518","display_name":"International Journal of Laboratory Hematology","issn_l":"1751-5521","issn":["1751-5521","1751-553X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Laboratory Hematology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014064112","display_name":"Friedel Nollet","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210123377","display_name":"AZ Sint-Jan","ror":"https://ror.org/030h1vb90","country_code":"BE","type":"healthcare","lineage":["https://openalex.org/I4210123377"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Friedel Nollet","raw_affiliation_strings":["Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium","institution_ids":["https://openalex.org/I4210123377"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010424713","display_name":"Karlien Vanhouteghem","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210123377","display_name":"AZ Sint-Jan","ror":"https://ror.org/030h1vb90","country_code":"BE","type":"healthcare","lineage":["https://openalex.org/I4210123377"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Karlien Vanhouteghem","raw_affiliation_strings":["Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium","institution_ids":["https://openalex.org/I4210123377"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013663477","display_name":"Stefanie Vermeire","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210123377","display_name":"AZ Sint-Jan","ror":"https://ror.org/030h1vb90","country_code":"BE","type":"healthcare","lineage":["https://openalex.org/I4210123377"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Stefanie Vermeire","raw_affiliation_strings":["Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium","institution_ids":["https://openalex.org/I4210123377"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039804919","display_name":"Ellen Maelbrancke","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210123377","display_name":"AZ Sint-Jan","ror":"https://ror.org/030h1vb90","country_code":"BE","type":"healthcare","lineage":["https://openalex.org/I4210123377"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Ellen Maelbrancke","raw_affiliation_strings":["Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium","institution_ids":["https://openalex.org/I4210123377"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074718085","display_name":"Jan Emmerechts","orcid":"https://orcid.org/0000-0002-6788-1506","observed_orcids":["https://orcid.org/0000-0002-6788-1506"]},"institutions":[{"id":"https://openalex.org/I4210123377","display_name":"AZ Sint-Jan","ror":"https://ror.org/030h1vb90","country_code":"BE","type":"healthcare","lineage":["https://openalex.org/I4210123377"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Jan Emmerechts","raw_affiliation_strings":["Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium","institution_ids":["https://openalex.org/I4210123377"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103828650","display_name":"Helena Devos","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210123377","display_name":"AZ Sint-Jan","ror":"https://ror.org/030h1vb90","country_code":"BE","type":"healthcare","lineage":["https://openalex.org/I4210123377"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Helena Devos","raw_affiliation_strings":["Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium","institution_ids":["https://openalex.org/I4210123377"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5002283362","display_name":"Barbara Cauwelier","orcid":"https://orcid.org/0000-0002-2264-7302","observed_orcids":["https://orcid.org/0000-0002-2264-7302"]},"institutions":[{"id":"https://openalex.org/I4210123377","display_name":"AZ Sint-Jan","ror":"https://ror.org/030h1vb90","country_code":"BE","type":"healthcare","lineage":["https://openalex.org/I4210123377"]}],"countries":["BE"],"is_corresponding":true,"raw_author_name":"Barbara Cauwelier","raw_affiliation_strings":["Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium"],"raw_orcid":"https://orcid.org/0000-0002-2264-7302","affiliations":[{"raw_affiliation_string":"Department of Laboratory Medicine, AZ Sint-Jan Brugge-Oostende, Bruges, Belgium","institution_ids":["https://openalex.org/I4210123377"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5002283362"],"corresponding_institution_ids":["https://openalex.org/I4210123377"],"apc_list":{"value":3880,"currency":"USD","value_usd":3880},"apc_paid":null,"fwci":0.2341,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.62573321,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":97},"biblio":{"volume":"41","issue":"2","first_page":"242","last_page":"249"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10185","display_name":"Lymphoma Diagnosis and Treatment","score":0.6664999723434448,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10185","display_name":"Lymphoma Diagnosis and Treatment","score":0.6664999723434448,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10950","display_name":"Acute Lymphoblastic Leukemia research","score":0.12280000001192093,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.0892999991774559,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/t-cell-receptor-gamma-gene-rearrangement","display_name":"T-cell receptor gamma gene rearrangement","score":0.9769999980926514},{"id":"https://openalex.org/keywords/clonality-analysis","display_name":"clonality analysis","score":0.9039999842643738},{"id":"https://openalex.org/keywords/t-cell-malignancies","display_name":"T-cell malignancies","score":0.921999990940094},{"id":"https://openalex.org/keywords/next-generation-sequencing","display_name":"next-generation sequencing","score":0.8690000176429749},{"id":"https://openalex.org/keywords/interpretation-algorithm","display_name":"interpretation algorithm","score":0.8330000042915344},{"id":"https://openalex.org/keywords/formalin-fixed-paraffin-embedded-tissue","display_name":"formalin-fixed paraffin-embedded tissue","score":0.9570000171661377},{"id":"https://openalex.org/keywords/lymphoproliferative-disorders","display_name":"lymphoproliferative disorders","score":0.9300000071525574},{"id":"https://openalex.org/keywords/sensitivity-and-specificity","display_name":"sensitivity and specificity","score":0.9419999718666077}],"concepts":[{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.7663723826408386},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.5716026425361633},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.4719994068145752},{"id":"https://openalex.org/C2993206177","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon sequencing","level":4,"score":0.4586314260959625},{"id":"https://openalex.org/C9893847","wikidata":"https://www.wikidata.org/wiki/Q1425625","display_name":"Reproducibility","level":2,"score":0.45447972416877747},{"id":"https://openalex.org/C2992511266","wikidata":"https://www.wikidata.org/wiki/Q1093098","display_name":"Gene rearrangement","level":3,"score":0.4520355463027954},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.44954484701156616},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3739517331123352},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.35371747612953186},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3441493511199951},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.19001257419586182},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.16515135765075684},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.09636983275413513},{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015321","descriptor_name":"Gene Rearrangement","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016692","descriptor_name":"Receptors, Antigen, T-Cell, gamma-delta","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019337","descriptor_name":"Hematologic Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1111/ijlh.12954","doi":"https://doi.org/10.1111/ijlh.12954","is_oa":false,"landing_page_url":"https://doi.org/10.1111/ijlh.12954","pdf_url":null,"source":{"id":"https://openalex.org/S66208518","display_name":"International Journal of Laboratory Hematology","issn_l":"1751-5521","issn":["1751-5521","1751-553X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International Journal of Laboratory Hematology","raw_type":"journal-article"},{"id":"pmid:30537135","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30537135","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"International journal of laboratory hematology","raw_type":"Evaluation Study"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.8999999761581421}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":8,"referenced_works":["https://openalex.org/W1981548401","https://openalex.org/W2020094108","https://openalex.org/W2020663892","https://openalex.org/W2053067769","https://openalex.org/W2084781910","https://openalex.org/W2113750306","https://openalex.org/W2123249056","https://openalex.org/W2333323072"],"related_works":["https://openalex.org/W2886066651","https://openalex.org/W7100773940","https://openalex.org/W3155786824","https://openalex.org/W4408982388","https://openalex.org/W4400928262","https://openalex.org/W2149951197","https://openalex.org/W2987266523","https://openalex.org/W4406167187","https://openalex.org/W2790066999","https://openalex.org/W4414740771"],"abstract_inverted_index":{"INTRODUCTION:":[0],"T-cell":[1,17,148,189,203],"receptor":[2],"gene":[3],"(TRG)":[4],"rearrangement":[5,59],"profiling":[6],"is":[7,32,37],"an":[8,70,206],"essential":[9],"component":[10],"of":[11,16,43,50,57,113,142,158,183,188,202],"the":[12,55,114,180,184,200],"workup":[13],"at":[14],"diagnosis":[15,101,201],"malignancies.":[18],"TRG":[19,58,97,115,128,134,166,192],"amplification":[20],"by":[21,28,39,61,94,165,191],"polymerase":[22],"chain":[23],"reaction":[24],"(PCR)":[25],"and":[26,46,79,96,111,133],"analysis":[27,60,117,157],"capillary":[29],"electrophoresis":[30],"(PCR-CE)":[31],"mostly":[33],"widely":[34],"used":[35],"but":[36],"hampered":[38],"a":[40,89,123,195],"subjective":[41],"interpretation":[42,49,73,125],"its":[44],"results":[45,138],"possible":[47],"false-positive":[48],"clonality.":[51],"Several":[52],"studies":[53],"evaluated":[54],"advantage":[56],"Next":[62],"Generation":[63],"Sequencing":[64],"(TRG-NGS),":[65],"however":[66],"few":[67],"have":[68],"proposed":[69],"adequate":[71],"data":[72,130],"algorithm.":[74],"METHODS:":[75],"Eighty":[76],"five":[77],"fresh":[78,106,143],"36":[80],"formalin-fixed":[81],"paraffin":[82],"embedded":[83],"(FFPE)":[84],"diagnostic":[85],"samples":[86],"suspected":[87],"for":[88,104,127],"lymphoproliferative":[90],"disorder":[91],"were":[92,150],"analyzed":[93],"PCR-CE":[95,132,173],"NGS.":[98],"Final":[99],"clinical":[100],"was":[102,118,161],"available":[103],"all":[105],"samples.":[107,144,220],"Reproducibility,":[108],"analytical":[109],"specificity":[110],"sensitivity":[112],"NGS":[116,129,135,167,193],"evaluated.":[119],"RESULTS:":[120],"We":[121],"propose":[122],"new":[124],"algorithm":[126],"analysis.":[131],"showed":[136],"identical":[137],"in":[139,170,199,211],"66/85":[140],"(78%)":[141],"Sensitivities":[145],"to":[146,172,179,208,213],"detect":[147],"malignancies":[149],"comparable":[151],"(96%":[152],"versus":[153],"92%,":[154],"respectively).":[155],"The":[156],"FFPE":[159,219],"material":[160],"significantly":[162],"more":[163],"successful":[164],"(34/36":[168],"cases)":[169],"respect":[171],"(16/36":[174],"cases),":[175],"most":[176],"likely":[177],"due":[178],"small":[181],"size":[182],"amplicons.":[185],"CONCLUSION:":[186],"Assessment":[187],"clonality":[190],"has":[194],"significant":[196],"added":[197],"value":[198],"disorders":[204],"as":[205],"adjunct":[207],"PCR-CE,":[209],"particularly":[210],"difficult":[212],"interpret":[214],"cases":[215],"or":[216],"when":[217],"analyzing":[218]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2018-12-22T00:00:00"}