{"id":"https://openalex.org/W2895816064","doi":"https://doi.org/10.1186/s12934-018-1009-5","title":"An improved secretion signal enhances the secretion of model proteins from Pichia pastoris","display_name":"An improved secretion signal enhances the secretion of model proteins from Pichia pastoris","publication_year":2018,"publication_date":"2018-10-12","ids":{"openalex":"https://openalex.org/W2895816064","doi":"https://doi.org/10.1186/s12934-018-1009-5","mag":"2895816064","pmid":"https://pubmed.ncbi.nlm.nih.gov/30314480","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6182871"},"language":"en","primary_location":{"id":"doi:10.1186/s12934-018-1009-5","doi":"https://doi.org/10.1186/s12934-018-1009-5","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12934-018-1009-5","pdf_url":"https://microbialcellfactories.biomedcentral.com/track/pdf/10.1186/s12934-018-1009-5","source":{"id":"https://openalex.org/S171368329","display_name":"Microbial Cell Factories","issn_l":"1475-2859","issn":["1475-2859"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbial Cell Factories","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://microbialcellfactories.biomedcentral.com/track/pdf/10.1186/s12934-018-1009-5","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5034754389","display_name":"Juan J. Barrero","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I123044942","display_name":"Universitat Aut\u00f2noma de Barcelona","ror":"https://ror.org/052g8jq94","country_code":"ES","type":"education","lineage":["https://openalex.org/I123044942"]},{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["ES","US"],"is_corresponding":false,"raw_author_name":"Juan J. Barrero","raw_affiliation_strings":["Department of Chemical, Biological and Environmental Engineering, Escola d'Enginyeria, Universitat Aut\u00f2noma de Barcelona, Bellaterra (Cerdanyola del Vall\u00e8s), 08193, Catalonia, Spain","Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL, 60637, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical, Biological and Environmental Engineering, Escola d'Enginyeria, Universitat Aut\u00f2noma de Barcelona, Bellaterra (Cerdanyola del Vall\u00e8s), 08193, Catalonia, Spain","institution_ids":["https://openalex.org/I123044942"]},{"raw_affiliation_string":"Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL, 60637, USA","institution_ids":["https://openalex.org/I40347166"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004589042","display_name":"Jason C. Casler","orcid":"https://orcid.org/0000-0001-9742-9978","observed_orcids":["https://orcid.org/0000-0001-9742-9978"]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jason C. Casler","raw_affiliation_strings":["Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL, 60637, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL, 60637, USA","institution_ids":["https://openalex.org/I40347166"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103276889","display_name":"Francisco Valero","orcid":"https://orcid.org/0000-0003-0429-9620","observed_orcids":["https://orcid.org/0000-0003-0429-9620","https://orcid.org/0000-0002-3721-0170","https://orcid.org/0000-0002-2095-4974"]},"institutions":[{"id":"https://openalex.org/I123044942","display_name":"Universitat Aut\u00f2noma de Barcelona","ror":"https://ror.org/052g8jq94","country_code":"ES","type":"education","lineage":["https://openalex.org/I123044942"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Francisco Valero","raw_affiliation_strings":["Department of Chemical, Biological and Environmental Engineering, Escola d'Enginyeria, Universitat Aut\u00f2noma de Barcelona, Bellaterra (Cerdanyola del Vall\u00e8s), 08193, Catalonia, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical, Biological and Environmental Engineering, Escola d'Enginyeria, Universitat Aut\u00f2noma de Barcelona, Bellaterra (Cerdanyola del Vall\u00e8s), 08193, Catalonia, Spain","institution_ids":["https://openalex.org/I123044942"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037504477","display_name":"Pau Ferrer","orcid":"https://orcid.org/0000-0002-5287-4127","observed_orcids":["https://orcid.org/0000-0002-5287-4127"]},"institutions":[{"id":"https://openalex.org/I123044942","display_name":"Universitat Aut\u00f2noma de Barcelona","ror":"https://ror.org/052g8jq94","country_code":"ES","type":"education","lineage":["https://openalex.org/I123044942"]},{"id":"https://openalex.org/I4210112527","display_name":"Luxembourg Institute of Science and Technology","ror":"https://ror.org/01t178j62","country_code":"LU","type":"nonprofit","lineage":["https://openalex.org/I4210112527"]}],"countries":["ES","LU"],"is_corresponding":false,"raw_author_name":"Pau Ferrer","raw_affiliation_strings":["Department of Chemical, Biological and Environmental Engineering, Escola d'Enginyeria, Universitat Aut\u00f2noma de Barcelona, Bellaterra (Cerdanyola del Vall\u00e8s), 08193, Catalonia, Spain","Luxembourg Institute of Science and Technology (LIST), Belvaux, Luxembourg"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical, Biological and Environmental Engineering, Escola d'Enginyeria, Universitat Aut\u00f2noma de Barcelona, Bellaterra (Cerdanyola del Vall\u00e8s), 08193, Catalonia, Spain","institution_ids":["https://openalex.org/I123044942"]},{"raw_affiliation_string":"Luxembourg Institute of Science and Technology (LIST), Belvaux, Luxembourg","institution_ids":["https://openalex.org/I4210112527"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5006514294","display_name":"Benjamin S. Glick","orcid":"https://orcid.org/0000-0002-7921-1374","observed_orcids":["https://orcid.org/0000-0002-7921-1374"]},"institutions":[{"id":"https://openalex.org/I40347166","display_name":"University of Chicago","ror":"https://ror.org/024mw5h28","country_code":"US","type":"education","lineage":["https://openalex.org/I40347166"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Benjamin S. Glick","raw_affiliation_strings":["Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL, 60637, USA. bsglick@uchicago.edu","Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL, 60637, USA"],"raw_orcid":"https://orcid.org/0000-0002-7921-1374","affiliations":[{"raw_affiliation_string":"Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL, 60637, USA. bsglick@uchicago.edu","institution_ids":["https://openalex.org/I40347166"]},{"raw_affiliation_string":"Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL, 60637, USA","institution_ids":["https://openalex.org/I40347166"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5006514294"],"corresponding_institution_ids":["https://openalex.org/I40347166"],"apc_list":{"value":3590,"currency":"USD","value_usd":3590},"apc_paid":{"value":3590,"currency":"USD","value_usd":3590},"fwci":4.1765,"has_fulltext":true,"cited_by_count":157,"citation_normalized_percentile":{"value":0.95794844,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"17","issue":"1","first_page":"161","last_page":"161"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12668","display_name":"Yeasts and Rust Fungi Studies","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-secretion","display_name":"protein secretion","score":0.7200000286102295},{"id":"https://openalex.org/keywords/secretion-signal","display_name":"secretion signal","score":0.7549999952316284},{"id":"https://openalex.org/keywords/komagataella-phaffii","display_name":"Pichia pastoris","score":0.953000009059906},{"id":"https://openalex.org/keywords/cotranslational-translocation","display_name":"cotranslational translocation","score":0.875},{"id":"https://openalex.org/keywords/endoplasmic-reticulum","display_name":"endoplasmic reticulum","score":0.8569999933242798},{"id":"https://openalex.org/keywords/protein-aggregation","display_name":"protein aggregation","score":0.671999990940094},{"id":"https://openalex.org/keywords/oligomeric-proteins","display_name":"oligomeric proteins","score":0.8059999942779541}],"concepts":[{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.8954538702964783},{"id":"https://openalex.org/C2781372317","wikidata":"https://www.wikidata.org/wiki/Q148697","display_name":"Pichia pastoris","level":4,"score":0.8927268981933594},{"id":"https://openalex.org/C156399914","wikidata":"https://www.wikidata.org/wiki/Q7444454","display_name":"Secretory protein","level":3,"score":0.641746461391449},{"id":"https://openalex.org/C10858879","wikidata":"https://www.wikidata.org/wiki/Q899790","display_name":"Signal peptide","level":4,"score":0.5463273525238037},{"id":"https://openalex.org/C2779703480","wikidata":"https://www.wikidata.org/wiki/Q125153","display_name":"Pichia","level":5,"score":0.5264932513237},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.38555020093917847},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3833993673324585},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.35840219259262085},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2924879491329193},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.23071253299713135},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.061996519565582275}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010843","descriptor_name":"Pichia","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D021382","descriptor_name":"Protein Sorting Signals","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":7,"locations":[{"id":"doi:10.1186/s12934-018-1009-5","doi":"https://doi.org/10.1186/s12934-018-1009-5","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12934-018-1009-5","pdf_url":"https://microbialcellfactories.biomedcentral.com/track/pdf/10.1186/s12934-018-1009-5","source":{"id":"https://openalex.org/S171368329","display_name":"Microbial Cell Factories","issn_l":"1475-2859","issn":["1475-2859"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbial Cell Factories","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:5136972","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC6182871","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:6182871","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6182871","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microb Cell Fact","raw_type":"Text"},{"id":"pmid:30314480","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30314480","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbial cell factories","raw_type":"Journal Article"},{"id":"pmh:oai:ddd.uab.cat:200901","doi":null,"is_oa":true,"landing_page_url":"https://ddd.uab.cat/record/200901","pdf_url":"https://ddd.uab.cat/record/200901","source":{"id":"https://openalex.org/S4306400380","display_name":"Dip\u00f2sit Digital de Documents de la UAB (Universitat Aut\u00f2noma de Barcelona)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I123044942","host_organization_name":"Universitat Aut\u00f2noma de Barcelona","host_organization_lineage":["https://openalex.org/I123044942"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"},{"id":"pmh:oai:dnet:uabarcelona_::99f687752ded382a83087bab8f7307eb","doi":null,"is_oa":true,"landing_page_url":"http://ddd.uab.cat/record/200901","pdf_url":"http://ddd.uab.cat/record/200901","source":{"id":"https://openalex.org/S4306402641","display_name":"LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Cient\u00edficas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4383465926","host_organization_name":"LA Referencia","host_organization_lineage":["https://openalex.org/I4383465926"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:doaj.org/article:13de45b2a14640ebb9f2d1fa7f2926bb","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/13de45b2a14640ebb9f2d1fa7f2926bb","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Microbial Cell Factories, Vol 17, Iss 1, Pp 1-13 (2018)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1186/s12934-018-1009-5","doi":"https://doi.org/10.1186/s12934-018-1009-5","is_oa":true,"landing_page_url":"https://doi.org/10.1186/s12934-018-1009-5","pdf_url":"https://microbialcellfactories.biomedcentral.com/track/pdf/10.1186/s12934-018-1009-5","source":{"id":"https://openalex.org/S171368329","display_name":"Microbial Cell Factories","issn_l":"1475-2859","issn":["1475-2859"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbial Cell Factories","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1656657801","display_name":"Dissecting the functions of yeast COPI","funder_award_id":"R01GM104010","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G6527744703","display_name":null,"funder_award_id":"CTQ2016-74959-R","funder_id":"https://openalex.org/F4320326262","funder_display_name":"Ministerio de Asuntos Econ\u00f3micos y Transformaci\u00f3n Digital, Gobierno de Espa\u00f1a"}],"funders":[{"id":"https://openalex.org/F4320321505","display_name":"Generalitat de Catalunya","ror":"https://ror.org/01bg62x04"},{"id":"https://openalex.org/F4320323428","display_name":"Universitat de Barcelona","ror":"https://ror.org/021018s57"},{"id":"https://openalex.org/F4320326262","display_name":"Ministerio de Asuntos Econ\u00f3micos y Transformaci\u00f3n Digital, Gobierno de Espa\u00f1a","ror":"https://ror.org/03sv46s19"},{"id":"https://openalex.org/F4320326826","display_name":"Universitat Aut\u00f2noma de Barcelona","ror":"https://ror.org/052g8jq94"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2895816064.pdf","grobid_xml":"https://content.openalex.org/works/W2895816064.grobid-xml"},"referenced_works_count":51,"referenced_works":["https://openalex.org/W66561613","https://openalex.org/W139305927","https://openalex.org/W829478918","https://openalex.org/W1966350336","https://openalex.org/W1966793537","https://openalex.org/W1969175564","https://openalex.org/W1987515987","https://openalex.org/W1989851713","https://openalex.org/W1990820170","https://openalex.org/W2008801986","https://openalex.org/W2010759673","https://openalex.org/W2011498028","https://openalex.org/W2019618958","https://openalex.org/W2026070522","https://openalex.org/W2037185463","https://openalex.org/W2039472102","https://openalex.org/W2041674095","https://openalex.org/W2046437093","https://openalex.org/W2047684202","https://openalex.org/W2050953035","https://openalex.org/W2051403769","https://openalex.org/W2064577218","https://openalex.org/W2066837313","https://openalex.org/W2069940872","https://openalex.org/W2077057721","https://openalex.org/W2092233698","https://openalex.org/W2093057097","https://openalex.org/W2094338137","https://openalex.org/W2095668363","https://openalex.org/W2112244637","https://openalex.org/W2112736223","https://openalex.org/W2115853386","https://openalex.org/W2118278390","https://openalex.org/W2133425057","https://openalex.org/W2134641279","https://openalex.org/W2136133782","https://openalex.org/W2145352278","https://openalex.org/W2147993636","https://openalex.org/W2152726884","https://openalex.org/W2167041195","https://openalex.org/W2169597956","https://openalex.org/W2170171321","https://openalex.org/W2234388732","https://openalex.org/W2265713480","https://openalex.org/W2274846837","https://openalex.org/W2336459044","https://openalex.org/W2418361432","https://openalex.org/W2512361232","https://openalex.org/W2544326521","https://openalex.org/W2604836961","https://openalex.org/W2797755099"],"related_works":["https://openalex.org/W3092520069","https://openalex.org/W4394839881","https://openalex.org/W7039578722","https://openalex.org/W2014728602","https://openalex.org/W2950446191","https://openalex.org/W2596653857","https://openalex.org/W2536241994","https://openalex.org/W7064679050","https://openalex.org/W7018868504","https://openalex.org/W2350109756"],"abstract_inverted_index":{"BACKGROUND:":[0],"Proteins":[1],"can":[2,67,90,263],"be":[3,73,257],"secreted":[4],"from":[5,49,97,249],"a":[6,83,113,146],"host":[7],"organism":[8],"with":[9,157,211,221,230],"the":[10,37,44,60,70,86,98,106,119,130,134,149,158,162,172,181,188,192,208,212,222,231,244,266],"aid":[11],"of":[12,29,47,105,118,180,187,201,246],"N-terminal":[13,45],"secretion":[14,41,54,108,115,170,197,200,215,229,233,238,245],"signals.":[15],"The":[16,194,236],"budding":[17],"yeast":[18],"Pichia":[19],"pastoris":[20],"(Komagataella":[21],"sp.)":[22],"is":[23,43],"widely":[24],"employed":[25],"to":[26,204,207,256],"secrete":[27],"proteins":[28,65,248,261],"academic":[30],"and":[31,76,139,268],"industrial":[32],"interest.":[33],"For":[34],"this":[35,53],"yeast,":[36],"most":[38,258],"commonly":[39],"used":[40],"signal":[42,55,116,123,164,174,198,239],"portion":[46],"pre-pro-\u03b1-factor":[48,107],"Saccharomyces":[50],"cerevisiae.":[51],"However,":[52],"promotes":[56,126],"posttranslational":[57],"translocation":[58,128],"into":[59,129],"endoplasmic":[61],"reticulum":[62],"(ER),":[63],"so":[64],"that":[66,262],"fold":[68,264],"in":[69,191,265],"cytosol":[71,267],"may":[72],"inefficiently":[74],"translocated":[75],"thus":[77],"poorly":[78],"secreted.":[79],"In":[80],"addition,":[81],"if":[82],"protein":[84,148,153],"self-associates,":[85],"\u03b1-factor":[87,135,159,173,182,214],"pro":[88,136,160,183],"region":[89,184],"potentially":[91],"cause":[92],"aggregation,":[93],"thereby":[94],"hampering":[95],"export":[96],"ER.":[99,193],"This":[100],"study":[101],"addresses":[102],"both":[103],"limitations":[104],"signal.":[109,216,234],"RESULTS:":[110],"We":[111],"engineered":[112],"hybrid":[114],"consisting":[117],"S.":[120],"cerevisiae":[121],"Ost1":[122,163],"sequence,":[124],"which":[125,225],"cotranslational":[127],"ER,":[131],"followed":[132],"by":[133],"region.":[137],"Secretion":[138],"intracellular":[140],"localization":[141],"were":[142,219],"assessed":[143],"using":[144],"as":[145],"model":[147],"tetrameric":[150],"red":[151],"fluorescent":[152],"E2-Crimson.":[154],"When":[155],"paired":[156],"region,":[161],"sequence":[165],"yielded":[166],"much":[167],"more":[168],"efficient":[169],"than":[171],"sequence.":[175],"Moreover,":[176],"an":[177],"allelic":[178],"variant":[179],"reduced":[185],"aggregation":[186],"E2-Crimson":[189,202],"construct":[190],"resulting":[195],"improved":[196,232,237],"enhanced":[199,228],"up":[203],"20-fold":[205],"compared":[206],"levels":[209],"obtained":[210,220],"original":[213],"Similar":[217],"findings":[218],"lipase":[223],"BTL2,":[224],"exhibited":[226],"10-fold":[227],"CONCLUSIONS:":[235],"confers":[240],"dramatic":[241],"benefits":[242,253],"for":[243,260,269],"certain":[247],"P.":[250],"pastoris.":[251],"These":[252],"are":[254],"likely":[255],"evident":[259],"oligomeric":[270],"proteins.":[271]},"counts_by_year":[{"year":2026,"cited_by_count":25},{"year":2025,"cited_by_count":25},{"year":2024,"cited_by_count":19},{"year":2023,"cited_by_count":18},{"year":2022,"cited_by_count":22},{"year":2021,"cited_by_count":29},{"year":2020,"cited_by_count":9},{"year":2019,"cited_by_count":9}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2018-10-26T00:00:00"}
